Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	657,541	G→T	intergenic (+40/+14)	cspE → / ← flc	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	657,541	0	G→T	100.0%	177.9	55	intergenic (+40/+14)	cspE/flc	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  new base (30/21):  ref base (2/2):  total (32/23)

GGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCA  >  NC_000913/657337‑657746                                                                                                                                                                                                             |                                                                                                                                                                                                               ggATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCCTAATCAGCGGTttttt                                                                                                                                                                                                           >  2:505911/1‑210 (MQ=255)      cGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTgg                                                                                                                                                                                                     >  1:423041/1‑210 (MQ=255)        ttttCATTACTCCGGAAGTCGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCTCGTAGAGTTCGAACTCACTAACGGTGCCAAATGCCCTTCTGCTGCAAACGTAATCGCTCTGGAAGATACATCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTc                                                                                                                                                                                                   <  1:277953/209‑1 (MQ=255)               tACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGACCCTTCTGCTGCAAACGTAAGCGCTCTGTAAGATACGTCAGCAAGAATTCAAACCCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAgtg                                                                                                                                                                                            >  2:581930/1‑210 (MQ=255)                 cTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGtgtg                                                                                                                                                                                          >  2:269645/1‑210 (MQ=255)                  tCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGc                                                                                                                                                                                         >  2:134422/1‑210 (MQ=255)                    cGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCgg                                                                                                                                                                                       >  2:619451/1‑210 (MQ=255)                         gACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAg                                                                                                                                                                                  >  2:255210/1‑210 (MQ=255)                           cGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATAACGAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGACAGCAAGCATTCAAAACCCGCCTAATCAGCGCTTTTTTC‑TTGGTCTTCAGTCGGCGGTTGAGGc                                                                                                                                                                                >  1:667930/1‑210 (MQ=255)                                 caAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGaaaa                                                                                                                                                                          >  1:138495/1‑210 (MQ=255)                                   gaGAAGTGTACGAACACGTCTTTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACa                                                                                                                                                                        >  2:608757/2‑210 (MQ=255)                                   aaGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTTCCAAAGGCCCTTCTGCTGCAAACGTAATCGATCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTATTGTGCGGTTGAGGCCTGAAACa                                                                                                                                                                        >  2:337198/1‑210 (MQ=255)                                         tgtTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGa                                                                                                                                                                  >  1:525103/1‑210 (MQ=255)                                            tCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATg                                                                                                                                                               <  1:451171/210‑1 (MQ=255)                                                         tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTTTTTATTTTTGGGTT‑AAGCCGGAAACAGCCAAGATGCCaagtcggttctg                                                                                                                                                 >  2:264495/1‑199 (MQ=255)                                                          gaaaTCCAGCCTAATGTTTTAAAACCACTGGCTGAAGGTCAGGGCGATAAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCGGCAAAAGTAACCCCCCTGTAGGTTAGTTCAGAAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg                                                                                                                                                 <  2:568500/208‑1 (MQ=255)                                                           cAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATgg                                                                                                                                                <  1:418401/210‑1 (MQ=255)                                                               ccAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaa                                                                                                                                            >  1:382983/1‑210 (MQ=255)                                                                           ttttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg                                                                                                                                >  2:342249/1‑210 (MQ=255)                                                                               aaaaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTg                                                                                                                            <  2:294887/210‑1 (MQ=255)                                                                                 aaCTATTGCTGAAGGTAAGCGCGTAGAGTTCGAATTCACTAACGGGGCCAAAGGACATTCTGCTGCAAACGTAATCGCTCTGTAATATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TGGGTCTTTAGTGTGCGGTTGAGGCCGAAATCAGCCAGAATGCCAGTGCGGTCATGGCATAAGACCCCAGAAGGTTGAc                                                                                                                          <  1:175505/210‑1 (MQ=255)                                                                                       tGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTTCGGTTGAGGCCGAAAAAAGCCAGAACTCCAGTGCGGTCATGGCCAAAGACTCCAGAAGATATACGAAAAc                                                                                                                    >  2:454485/1‑210 (MQ=255)                                                                                       tGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAAc                                                                                                                    >  1:82633/1‑210 (MQ=255)                                                                                                        tAGAGTTCGAAAGCACTAACGGTGCCCAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATAAGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGc                                                                                                   <  1:405669/210‑1 (MQ=255)                                                                                                            gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCaaa                                                                                               >  1:397715/1‑210 (MQ=255)                                                                                                              tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGCCGAAAACGTTCAGTAATGCCCAGCCAAAGc                                                                                             >  2:166653/1‑210 (MQ=255)                                                                                                                gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCgg                                                                                           >  1:222720/1‑210 (MQ=255)                                                                                                                          aCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTa                                                                                 >  1:650377/1‑210 (MQ=255)                                                                                                                                ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaaa                                                                           >  1:624122/1‑210 (MQ=255)                                                                                                                                  aaaGGCCCTTCTTCTGCAAACGTAATCTCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAac                                                                         <  2:525076/210‑1 (MQ=255)                                                                                                                                   aaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAaca                                                                        <  1:437278/210‑1 (MQ=255)                                                                                                                                   aaGGCCCTTCTGCTGCAAACGTAAGCGCTCTGTAAGATACGTCAGCAAGAATTCAACACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAaca                                                                        <  2:423023/210‑1 (MQ=255)                                                                                                                                       ctcTTCTGCTGCAAACTTAATCGCTCTTTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcaccac                                                                    <  1:166659/208‑1 (MQ=255)                                                                                                                                        ccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACt                                                                   <  2:506865/210‑1 (MQ=255)                                                                                                                                        ccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACt                                                                   >  1:225332/1‑210 (MQ=255)                                                                                                                                         cTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACtt                                                                  >  2:493298/1‑210 (MQ=255)                                                                                                                                            ctgctgCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTg                                                               <  1:269654/210‑1 (MQ=255)                                                                                                                                                 gcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGaa                                                          >  1:589672/1‑210 (MQ=255)                                                                                                                                                     acgttatagacctGTAAGACACGTCAGGAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATg                                                      <  2:175978/199‑1 (MQ=255)                                                                                                                                                         aaTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTga                                                  <  1:619488/210‑1 (MQ=255)                                                                                                                                                           tCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTgaga                                                >  2:20804/1‑210 (MQ=255)                                                                                                                                                                 tGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGgttg                                          <  1:608793/210‑1 (MQ=255)                                                                                                                                                                  gcaaGATACGTCAGAAAGACATCAAAACACGCGTAATCACGGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGgttgt                                         <  2:328551/208‑1 (MQ=255)                                                                                                                                                                   tAAGATACGTCAGCAAGAATTCAAAGCCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGgttgtt                                        <  1:505938/210‑1 (MQ=255)                                                                                                                                                                    aacaTACCTCAGCAAGACTTCACACCCCGCTTACTCAGCGCTTCTTGA‑TTGGTCTTTAGTGTGCGGTGTAGGCAGAAACCAGCCAGAATGCCAGTGCGGTCATGGCAAAAGCCCCCAGAACGTTGACGACAACGTCCAGTCATGCCCAGCCAAAGCGGCACTCTTGTAACAAACACACCACTTCTGCCGAAAATGTTAAGAAGGTTGTTa                                       <  1:102195/207‑1 (MQ=255)                                                                                                                                                                           gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTATTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCTCTCTTGTAAAAAACACAACACTTATGCCGAAATAGGTAAGAAGGTTGTTGGAcggac                                >  1:196611/1‑206 (MQ=255)                                                                                                                                                                              aGCAAGAATTCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCcaca                             >  2:94872/1‑210 (MQ=255)                                                                                                                                                                                  accaaaaaaaaACCGCTGATTAAGCGGGTTTTTTGTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAAAGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCTCAGCCAAAGCGTGCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACaaaa                          >  1:414701/8‑210 (MQ=255)                                                                                                                                                                                     aaaaaaaaACCGCTGATTAAGCGGGTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGATCAGTAATGCGCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGCAAATGTTGAGAAGGTTGTTAGACCTCCACAAAATCCg                      >  1:497029/5‑210 (MQ=255)                                                                                                                                                                                       tCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAACATCCggt                    >  1:399104/1‑210 (MQ=255)                                                                                                                                                                                       tCAAAACCCGCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCggt                    <  1:255219/210‑1 (MQ=255)                                                                                                                                                                                        cAAACCCGGTTTAATCAGC‑GGTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCggtgg                  <  1:264504/210‑1 (MQ=255)                                                                                                                                                                                                gCTTAATCAGCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTaa           >  2:236774/1‑210 (MQ=255)                                                                                                                                                                                                       cAGCGGGTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTc    <  2:497003/210‑1 (MQ=255)                                                                                                                                                                                                         gCGGTTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCca  <  1:20805/210‑1 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                               GGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTT‑TTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCA  >  NC_000913/657337‑657746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.