Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,212,790	G→T	P58Q (CCG→CAG)	yehT ←	putative response regulator in two‑component system withYehU

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,212,790	0	G→T	100.0%	142.2	39	P58Q (CCG→CAG)	yehT	putative response regulator in two‑component system withYehU
Reads supporting (aligned to +/- strand):  new base (19/19):  ref base (0/1):  total (19/20)

GAAACATCCTGCTTGCTGCGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTC  >  NC_000913/2212589‑2212996                                                                                                                                                                                                          |                                                                                                                                                                                                               gAAACATCCTGCTTGCTGCGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTgg                                                                                                                                                                                                        <  1:136490/210‑1 (MQ=255)        ccTGCTTGCTGCGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCga                                                                                                                                                                                                 <  2:273207/210‑1 (MQ=255)           gcttgctGCGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGaa                                                                                                                                                                                              <  2:233211/210‑1 (MQ=255)           ccttgttgcgactctaaCGCAATCGCACAAGCCTTTCCTACAGTCGCGCTTCATCAATTGGCTTCATCAGATAATCGAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGaa                                                                                                                                                                                              <  2:595319/194‑1 (MQ=255)              tgctgcGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACa                                                                                                                                                                                           <  1:240635/210‑1 (MQ=255)              tgacgcgcTACTGACGCAATCGCGCCAGCTTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTAGTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACa                                                                                                                                                                                           <  2:148990/206‑1 (MQ=255)               gctgcGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAg                                                                                                                                                                                          >  2:311702/1‑210 (MQ=255)                      cgcTGGCGCGATTGCGTCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACAttc                                                                                                                                                                                   >  1:151293/3‑208 (MQ=255)                            cGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATATTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAACCAGCACTTCCGGgcgc                                                                                                                                                                             >  1:215551/1‑210 (MQ=255)                                aTCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGtt                                                                                                                                                                         >  1:140381/1‑210 (MQ=255)                                  cgcgCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTa                                                                                                                                                                       <  2:238438/210‑1 (MQ=255)                                       cAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCAc                                                                                                                                                                  <  1:651566/210‑1 (MQ=255)                                         gCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGCTTATGCACcg                                                                                                                                                                >  1:335204/1‑210 (MQ=255)                                                 tccggttgcgtgtcatcaATTGGCTTCAGCAGATAATAAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATc                                                                                                                                                        <  2:44690/198‑1 (MQ=255)                                                   cAGTCGCGCTTCATCAATTTGCTTCAGCAGTTAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGGTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATccc                                                                                                                                                      <  2:608759/210‑1 (MQ=255)                                                    aGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCt                                                                                                                                                     >  1:606604/1‑210 (MQ=255)                                                          gcTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACg                                                                                                                                               >  1:566048/1‑210 (MQ=255)                                                                  aaTTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGa                                                                                                                                       >  1:340157/1‑210 (MQ=255)                                                                  aaTTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGa                                                                                                                                       >  2:600833/1‑210 (MQ=255)                                                                        cTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACActc                                                                                                                                 <  2:350680/210‑1 (MQ=255)                                                                        cTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACActc                                                                                                                                 >  1:650323/1‑210 (MQ=255)                                                                          tCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGAACCGCGCCGATCCCTTCCACGGCTTTTGAACCctccc                                                                                                                               >  1:340856/1‑208 (MQ=255)                                                                                gATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACAACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGa                                                                                                                         >  1:453461/1‑210 (MQ=255)                                                                                               gTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGctct                                                                                                          >  1:177170/1‑210 (MQ=255)                                                                                                   ttcAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTc                                                                                                      >  2:564200/1‑210 (MQ=255)                                                                                                           cTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCaaaa                                                                                              >  2:582688/1‑210 (MQ=255)                                                                                                                             cAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTccc                                                                            >  2:128356/1‑210 (MQ=255)                                                                                                                               aaCGCAGTGTGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACCCTCCAACGATTTCAATATCGCTGTGTCCCTGCAAAAATACACGCAGGTTATCCTGt                                                                          >  1:63675/1‑210 (MQ=255)                                                                                                                                aCGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTg                                                                         <  2:606569/210‑1 (MQ=255)                                                                                                                                       gagaAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTCCTCCCGTGCTAACgg                                                                  >  1:362579/1‑210 (MQ=255)                                                                                                                                        agaAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTTCTAACGGt                                                                 >  2:658201/1‑210 (MQ=255)                                                                                                                                           aaaaCAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTtca                                                              <  1:194902/210‑1 (MQ=255)                                                                                                                                             aaCAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTtcatc                                                            <  1:420725/210‑1 (MQ=255)                                                                                                                                              acattatagggacggatacgcGGCTCAGGCATCCCCAGCATTACCATCCTAATGATGCGCGGCATCTGGATATCGAGACGCAGGCCATACGGGCGCAGTCTATGCTCCGCGCCGATCCCTTCAAAGGCGTTTGAACACTCTCCAGCGATTTCAAGATCGCTCTGCTCCTGCAAAAATACACGCAGTTGATCCCGTGCTAACGGTgcatca                                                           <  2:176855/190‑1 (MQ=255)                                                                                                                                                 atatACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCg                                                        <  2:177164/210‑1 (MQ=255)                                                                                                                                                        ggCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTa                                                 <  1:311716/210‑1 (MQ=255)                                                                                                                                                                   gggTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATc                                      <  1:503245/210‑1 (MQ=255)                                                                                                                                                                                                    aTGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTa     <  1:564229/210‑1 (MQ=255)                                                                                                                                                                                                      gcgcTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTAtt   <  2:225108/210‑1 (MQ=255)                                                                                                                                                                                                       cgctGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTc  <  2:58326/210‑1 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                               GAAACATCCTGCTTGCTGCGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGGTAGTCGTAACGTTATTC  >  NC_000913/2212589‑2212996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.