Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,212,790	G→T	P58Q (CCG→CAG)	yehT ←	putative response regulator in two‑component system withYehU

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,212,790	0	G→T	100.0%	103.3	31	P58Q (CCG→CAG)	yehT	putative response regulator in two‑component system withYehU
Reads supporting (aligned to +/- strand):  new base (17/13):  ref base (0/0):  total (18/13)

TTGCTGCGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGG  >  NC_000913/2212601‑2212979                                                                                                                                                                                              |                                                                                                                                                                                              ttgctGCGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAAc                                                                                                                                                                           <  1:538128/210‑1 (MQ=255)      gcgcTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGcac                                                                                                                                                                      <  2:416511/210‑1 (MQ=255)        gcTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGCTATCGATAAACAGCACAt                                                                                                                                                                    >  1:67493/1‑210 (MQ=255)              gACGCAATCGCGCCAGCGTTCTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTGTAATAGCGTATTCGTCAAACGCAGAGAAAAAAACAATATACGTGCGATGTTCCGAGTCCAGCATCCCCCCCATTCCCAGACAACTGATGCCCTCCATCGCGATATCTCGAACCACCCCATCCgcac                                                                                                                                                              >  1:417011/1‑207 (MQ=255)                     tCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGttt                                                                                                                                                       <  1:283936/210‑1 (MQ=255)                             gCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCCGCATCTGGATCTCGAGAAACAGCACATCCGGGCGCAGTTTATGAACcg                                                                                                                                               >  1:270554/1‑210 (MQ=255)                                ttttCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCgcgc                                                                                                                                            >  2:435306/1‑210 (MQ=255)                                    ctcCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTCTTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCACTGCATCTGGATATCGAGAAACAGCACATCCCGGCGCAGATTATGCACCGCCCCGac                                                                                                                                        >  1:445124/1‑209 (MQ=255)                                     tcCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCCAAGCTTATGCACCGCGCCGATc                                                                                                                                       >  1:415713/1‑210 (MQ=255)                                                   atcaATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGtt                                                                                                                         >  2:169046/1‑210 (MQ=255)                                                     caATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTg                                                                                                                       <  1:197908/210‑1 (MQ=255)                                                          ggCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAacac                                                                                                                  >  2:444993/1‑210 (MQ=255)                                                                   agATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACg                                                                                                         <  2:337066/210‑1 (MQ=255)                                                                        aTCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTc                                                                                                    >  2:529056/1‑210 (MQ=255)                                                                                     tcttcAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCACTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGc                                                                                       >  2:205678/1‑210 (MQ=255)                                                                                                 ttAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAAt                                                                           <  1:174626/210‑1 (MQ=255)                                                                                                    aTTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCCCTGCTCCTGCAAAAATaca                                                                        >  1:223771/1‑210 (MQ=255)                                                                                                          tATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAACAATACACGCAgg                                                                  >  1:2613/1‑210 (MQ=255)                                                                                                                 cAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCCccc                                                           >  1:147934/1‑210 (MQ=255)                                                                                                                             gaacagcagtataCGAGCGATTTTCCGGGTCAAGCATCCCCACCATTTCCAGACCAATGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGtt                                               <  1:86641/201‑1 (MQ=255)                                                                                                                               aaaaCAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTtca                                             <  1:52365/210‑1 (MQ=255)                                                                                                                                aaaCAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTtcat                                            <  2:67497/210‑1 (MQ=255)                                                                                                                                     ataaccgggccacattCCGGGTCTAGCGTCCCCACCATTTTCAGTCCACTGATGCACTGCCTCTTGATATCGGGAAACAAAACTTCCGGGCGCAGTGTATGGCCCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCATGTTCTCCCGTGCTAACGGTGCATCATCg                                       <  1:118628/196‑1 (MQ=255)                                                                                                                                            ggCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTa                                <  1:435283/210‑1 (MQ=255)                                                                                                                                              cGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAg                              >  1:40133/1‑210 (MQ=255)                                                                                                                                                   ttCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCCTCGACAATTAAGACttt                         >  1:133915/1‑210 (MQ=255)                                                                                                                                                      cGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAAt                      >  2:337842/1‑210 (MQ=255)                                                                                                                                                            aaGCATCCCCACCATTTCCAGACCACTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGcc                >  1:510482/1‑210 (MQ=255)                                                                                                                                                              gCATCCCCACCATTTCCAGACCGCTGATGCGCTGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTc              >  2:66797/1‑210 (MQ=255)                                                                                                                                                                 tCCCCACCAATTCCCGGCCACTGATGCGCTGCATCTGGATATCGGGAAACAGCACATCCGGGCTCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTc           <  1:437965/210‑1 (MQ=255)                                                                                                                                                                          tttCCCGACCACTGATGCGCTGCAGCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGGTCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATgg  <  1:34522/210‑1 (MQ=255)                                                                                                                                                                                              |                                                                                                                                                                                              TTGCTGCGCTCCTGACGCAATCGCGCCAGCGTTTTCTCCAGTCGCGCTTCATCAATTGGCTTCAGCAGATAATCAAAGGCATGTTCTTCAAAGGCTTTAATTGCGTATTCGTCAAACGCAGTGAGAAAAACAATATACGGGCGATGTTCCGGGTCAAGCATCCCCACCATTTCCAGACCACTGATGCGCGGCATCTGGATATCGAGAAACAGCACATCCGGGCGCAGTTTATGCACCGCGCCGATCCCTTCCACGGCGTTTGAACACTCTCCAACGATTTCAATATCGCTCTGCTCCTGCAAAAATACACGCAGGTTCTCCCGTGCTAACGGTTCATCATCGACAATTAAGACTTTAATCATGCCTCGTCCCTCCATGG  >  NC_000913/2212601‑2212979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.