Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,289,915	0	A→C	100.0%	0.6	1	F56C (TTT→TGT)	insH1	IS5 transposase and trans‑activator
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/1):  ref base (0/0):  total (0/1)

TTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCAT  >  NC_000913/2289906‑2290115          |                                                                                                                                                                                                         gtGGGGTAACACGGCGTGATGACTTCAACCATGGTGTGCCATGGCAGCATCTCCGCCTCGCGGGACAAGAGAATCGCTTATCGGGTGTGTGGGCGCTTACTGATGAATTCAATGTCGGCGAAGGTAACTTGATGAATTATGCTGAACGCTCTACTATGGCTCCAGATGACAAACACGCTCTCAGATCAGGGACTTGTTCGCACCTTCCAt  <  1:344331/209‑1 (MQ=255)          |                                                                                                                                                                                                         TTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCAT  >  NC_000913/2289906‑2290115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.