Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009132,289,9220C→T100.0% 0.1 2E54K (GAG→AAG) insH1IS5 transposase and trans‑activator
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/1):  ref base (0/1):  total (0/2)

TTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAG  >  NC_000913/2289906‑2290125
                |                                                                                                                                                                                                           
gtGGGGTAACACGGCGTGATGACTTCAACCATGGTGTGCCATGGCAGCATCTCCGCCTCGCGGGACAAGAGAATCGCTTATCGGGTGTGTGGGCGCTTACTGATGAATTCAATGTCGGCGAAGGTAACTTGATGAATTATGCTGAACGCTCTACTATGGCTCCAGATGACAAACACGCTCTCAGATCAGGGACTTGTTCGCACCTTCCAt            <  1:344331/209‑1 (MQ=255)
          aCGGCTCGATGACTTTCACCATGTTTTGCCATTGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAg  <  2:299117/210‑1 (MQ=25)
                |                                                                                                                                                                                                           
TTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAG  >  NC_000913/2289906‑2290125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.