Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,151	A→G	intergenic (‑18/+362)	metT ← / ← asnB	tRNA‑Met/asparagine synthetase B

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	697,151	0	A→G	100.0%	117.9	33	intergenic (‑18/+362)	metT/asnB	tRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand):  new base (15/17):  ref base (0/0):  total (16/17)

ACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGA  >  NC_000913/696948‑697333                                                                                                                                                                                                             |                                                                                                                                                                                       aCGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTAt                                                                                                                                                                                  >  2:220474/1‑210 (MQ=255)   gCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAATAGATGGTGGCTACGACGTGATTCGAACCTGTGACCCCTTCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑GTCCTTATCg                                                                                                                                                                                >  1:19072/1‑210 (MQ=255)   gCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCg                                                                                                                                                                                >  2:431031/1‑210 (MQ=255)                       ggAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATg                                                                                                                                                            >  2:450020/1‑210 (MQ=255)                                      aCTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCtttc                                                                                                                                             >  2:445343/1‑208 (MQ=255)                                       cTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCa                                                                                                                                            <  2:243291/210‑1 (MQ=255)                                       cTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCa                                                                                                                                            >  2:151597/1‑210 (MQ=255)                                                          cAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCaa                                                                                                                         >  2:368160/1‑210 (MQ=255)                                                           aGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAg                                                                                                                        <  2:378801/210‑1 (MQ=255)                                                           aGAACCTAAATCTGGTGCGTCGACCAATTTCGCCACTACCGAAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAg                                                                                                                        <  1:267588/210‑1 (MQ=255)                                                                     tCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCGCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGT‑TGCCTTATCGGCGTTGCCGGGCGCATTCTGCGTATTGAGCCTTGCAGCTTCACCCCCTTTTTCAAGGAAAAATggt                                                                                                              >  2:167982/1‑208 (MQ=37)                                                                               cTACCAAATCCGCCACTCCCGCACAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGAc                                                                                                    <  1:12681/210‑1 (MQ=255)                                                                                                cccGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCg                                                                                   <  1:443167/210‑1 (MQ=255)                                                                                                  cGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGaa                                                                                 >  1:452596/1‑210 (MQ=255)                                                                                                          aaGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTgg                                                                         <  2:40721/210‑1 (MQ=255)                                                                                                             aTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGgcgc                                                                      <  1:368183/210‑1 (MQ=255)                                                                                                                   gCTACGACGGGTTTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATAtt                                                                >  1:440353/1‑210 (MQ=255)                                                                                                                     tACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCg                                                              <  1:431058/210‑1 (MQ=255)                                                                                                                      acgacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGCTTTTTTTT‑TCGATTGCCTTAGTGGCGGTGTGCGAAGCATGATGCGTCTGGATCCTAACCGGGTGCATCACTTCTTGAAGGTACCGTGCTCGCAGGTGACTGTTTGGTTAGGTTGCGAACAGTGTGGGGCttcactcga                                                             >  2:40640/1‑202 (MQ=9)                                                                                                                               ttCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTg                                                    >  1:459480/1‑210 (MQ=255)                                                                                                                                 cGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGTGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTGGGTTAGGTTGCGGACAGCGTGGAGCTATATTCGTCAATTATTGtt                                                  >  1:167514/1‑210 (MQ=255)                                                                                                                                          gACCCCATCATTATGAGTGATGTGCTCTAACCACCTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTtgtg                                         <  2:25681/210‑1 (MQ=255)                                                                                                                                           aCCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTgtgt                                        <  1:283740/210‑1 (MQ=255)                                                                                                                                             cccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGttt                                      >  1:249998/1‑210 (MQ=255)                                                                                                                                                  catTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTcc                                 <  1:40640/210‑1 (MQ=255)                                                                                                                                                  catTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGATTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTcc                                 <  1:93057/210‑1 (MQ=255)                                                                                                                                                      aTGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCAcc                             <  1:450049/210‑1 (MQ=255)                                                                                                                                                       tGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCAccc                            <  1:386661/210‑1 (MQ=255)                                                                                                                                                                     tAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTAGATTCGTCAATTCTTGTTTCATTTGTGTTTTTTGCCACCCTACAGCCATTCttt              >  2:176452/1‑210 (MQ=255)                                                                                                                                                                       aCCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTg            <  1:445370/210‑1 (MQ=255)                                                                                                                                                                        ccAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCTCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGt           <  1:464656/210‑1 (MQ=255)                                                                                                                                                                                 cTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTTTCTTACAGGa  >  2:326627/1‑210 (MQ=255)                                                                                                                                                                                 cTACGTAGCCATCTTTTTTTTCGCGA‑TGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGa  <  1:347818/210‑1 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                       ACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGA‑TACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGA  >  NC_000913/696948‑697333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.