Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,583,939	0	G→T	100.0%	‑0.1	1	S88I (AGC→ATC)	insB1	IS1 transposase B
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (1/0):  ref base (0/0):  total (1/0)

TATTCGGTGAACGCACTATGGCGACGCTGGGGCGTCTTATGAGCCTGCTGTCACCCTTTGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGC  >  NC_000913/3583897‑3584106                                           |                                                                                                                                                                        tATTCGGTGAACGCACTATGGCGACGCTGGGGCGTCTTATGATCCTGCTGTCACCCTTTGACGTGGTGATCTGGATGACGGATGGCCAGCCGCTGTACGAATACCGCCTGAAGAGAAAGCAGCACGTAATGAGCAAGCGACATACGCAGCGTATTGAGCGGCATCACCTGAATCTTAGGCCGCACCTGACatccgcagagcacacgtctt  >  2:141710/1‑191 (MQ=1)                                           |                                                                                                                                                                        TATTCGGTGAACGCACTATGGCGACGCTGGGGCGTCTTATGAGCCTGCTGTCACCCTTTGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGAATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGC  >  NC_000913/3583897‑3584106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.