Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,151	A→G	intergenic (‑18/+362)	metT ← / ← asnB	tRNA‑Met/asparagine synthetase B

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	697,151	0	A→G	100.0%	147.0	41	intergenic (‑18/+362)	metT/asnB	tRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand):  new base (22/19):  ref base (0/0):  total (22/19)

CAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAAT  >  NC_000913/696946‑697354                                                                                                                                                                                                              |                                                                                                                                                                                                            cAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCtt                                                                                                                                                                                                         >  2:421071/1‑210 (MQ=255)             gTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCg                                                                                                                                                                                             >  2:274920/1‑210 (MQ=255)                       cGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTa                                                                                                                                                                                   <  2:304264/210‑1 (MQ=255)                           aGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCg                                                                                                                                                                               <  2:262271/210‑1 (MQ=255)                             gCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGta                                                                                                                                                                             >  1:49467/1‑210 (MQ=255)                              cGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGtat                                                                                                                                                                            <  2:479684/210‑1 (MQ=255)                               gagaCTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGtata                                                                                                                                                                           >  2:144452/1‑210 (MQ=255)                                 gaCTTGAACTCGCCCACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATGTCACCACTCCCGCAAACAAAGTGGGTGGCTACGCAGCGAGCCGAACCTGTATCCCCATCATTATGACTGCTGTGCGATACCCAACTGAGCGCCGACGCGACCTGTTTTCTTGGGCTGCCTTATCGTCGTTGCgggacccgtcgtacgtttact                                                                                                                                                                         >  1:159748/1‑192 (MQ=255)                                   cTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAACAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGc                                                                                                                                                                       >  2:309437/1‑210 (MQ=255)                                     tGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCt                                                                                                                                                                     <  2:453780/210‑1 (MQ=255)                                      gAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCAGTATGCGTATAGAGCCtt                                                                                                                                                                    >  1:388074/1‑210 (MQ=255)                                                  cTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAAcc                                                                                                                                                        <  2:132687/210‑1 (MQ=255)                                                      ccgggcCAGAACCTAAATCTGGTGCGTATACCAATTTCGCCACTCCCGAAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCtt                                                                                                                                                    <  2:99188/206‑1 (MQ=255)                                                        gcgcAAGAACCTAAATCTGGTGCGTATACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCtttt                                                                                                                                                  <  2:442680/210‑1 (MQ=255)                                                             aGAACCTAAATCGGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAg                                                                                                                                             <  2:449943/210‑1 (MQ=255)                                                                         tGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCg                                                                                                                                 >  2:523301/1‑210 (MQ=255)                                                                                          tCGCCTCTCCCCCAAAAAAATTTGGTGGCTACGACGGGATTCGAACCTGGGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGtt                                                                                                                <  2:16732/210‑1 (MQ=255)                                                                                              cACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGTAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGt                                                                                                            >  1:349028/1‑210 (MQ=255)                                                                                              cACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGt                                                                                                            >  2:498532/1‑210 (MQ=255)                                                                                                   ccGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGa                                                                                                       <  2:433828/210‑1 (MQ=255)                                                                                                    cGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGAGCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTGTCGGCGTTGCGGGGGGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTGTGAAGGAAAATTGCACGAAAGTGACTGTTTGGTTAGGTTGCGaa                                                                                                      >  2:111258/1‑210 (MQ=255)                                                                                                      cAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTGTGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGGTGCCTTATCGGCGGTGCGGGGCGAATTATGCGTATATGGCGTGTCCGGGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGGGTGTTTGGTTAGTGTGCGGACa                                                                                                    >  1:314659/1‑210 (MQ=25)                                                                                                          aaaaGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGt                                                                                                >  2:105495/1‑210 (MQ=255)                                                                                                              gATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGgcg                                                                                            >  2:441540/1‑210 (MQ=255)                                                                                                                       tACGACGGGATTCGACACTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGGGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCg                                                                                   <  1:231775/210‑1 (MQ=255)                                                                                                                       tACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCg                                                                                   >  1:113499/1‑210 (MQ=255)                                                                                                                        acgacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGt                                                                                  <  1:421043/210‑1 (MQ=255)                                                                                                                          gaccGGATTCGAACCTGTGAACCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCa                                                                                <  2:248855/210‑1 (MQ=255)                                                                                                                                   cGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGtt                                                                       >  2:531536/1‑210 (MQ=255)                                                                                                                                              ccccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGtt                                                            >  2:93911/1‑210 (MQ=255)                                                                                                                                                    tatTATGAGTGCTGTGCACTAACCAACTGAGCTACGTAGCCGTCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTcc                                                      <  2:173033/209‑1 (MQ=255)                                                                                                                                                                 gtgCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGTTTAGGTTGCGAACATCGTGGCGCTATATTCTGCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCa                                         >  1:478861/1‑210 (MQ=255)                                                                                                                                                                        aaCCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCCTTCtttt                                  >  1:372327/1‑210 (MQ=255)                                                                                                                                                                            aaCTGGGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCa                              <  1:105491/210‑1 (MQ=255)                                                                                                                                                                              cTGAGCTACGTAGCCATCTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATa                            >  2:203460/1‑210 (MQ=255)                                                                                                                                                                                             aTCTTTTTTTTCGCGATGCCGTATCGGCGTTGCGGGGCGCATTATGCGTATAGCGCAGTGCAGCGTCAACCTCTGTTTTAAGGAAAATTTCTCGAATGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTCCAGCCATTCTTTTGTCATACAGGATGAAATTCgg             <  1:101529/210‑1 (MQ=255)                                                                                                                                                                                               tttttttgtCGCGAGGCCTTAGCGGCGTTGCGGGGCGGATTGTGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTTTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGaa           <  1:134650/209‑1 (MQ=255)                                                                                                                                                                                               cTTTTTTTTCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGaa           <  1:274907/210‑1 (MQ=255)                                                                                                                                                                                                   tttttCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTa       <  2:89804/210‑1 (MQ=255)                                                                                                                                                                                                     tttCGCGATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAAc     >  2:469335/1‑210 (MQ=255)                                                                                                                                                                                                        cgcgATGCCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACCGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAAt  >  1:337428/1‑210 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                            CAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTCCCACCCTACAGCCATTCTTTTGTCATACAGGATGAAATTCGGAATTTAACAAT  >  NC_000913/696946‑697354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.