Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,548	C→G	N214K (AAC→AAG)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	756,548	0	C→G	100.0%	76.6	23	N214K (AAC→AAG)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  new base (10/11):  ref base (0/0):  total (10/13)

ACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGA  >  NC_000913/756348‑756751                                                                                                                                                                                                         |                                                                                                                                                                                                            acGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACAtt                                                                                                                                                                                                    <  2:253931/210‑1 (MQ=255)  cGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTa                                                                                                                                                                                                   >  2:60457/1‑210 (MQ=255)                                         tctcCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCg                                                                                                                                                            >  2:281004/1‑210 (MQ=255)                                                                tcttccgatctccAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAAtg                                                                                                                                     <  2:256328/199‑1 (MQ=255)                                                                    tGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGc                                                                                                                                 >  2:451547/1‑210 (MQ=255)                                                                           ccAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCAACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGTATATGGAAAtgagatcggaaga                                                                                                                          >  1:256319/1‑199 (MQ=255)                                                                                 tGGCGCGGTAGTGGGTTATACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTAGTTCAAAGCCCGCGCTACCGTCCTGGCGCCGGGCGGATCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACAGCGGCGACGGAGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGAc                                                                                                                    <  2:164008/210‑1 (MQ=255)                                                                                       ggtggtGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCAt                                                                                                              <  2:315923/210‑1 (MQ=255)                                                                                                ttGTACCGCAGGGTGCTGCGAAACCGGTGGAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTAGCGGAGCAGGGCGTATTTATCAGTCCACCACCAATGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGTATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGc                                                                                                     <  2:201685/210‑1 (MQ=255)                                                                                                ttGTACCCCACTGTGCATCGAAACCGGTGAAGTTGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGc                                                                                                     <  2:383565/210‑1 (MQ=255)                                                                                                    aCCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCggcc                                                                                                 >  1:106716/1‑208 (MQ=255)                                                                                                                 tCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACGGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTACCCGTGCCGGCGTACCGGTGCAGGATAGGGGAAGGTGGCCGGTCCACCCGACCGGCATTGCCGGGGCGGGCGGAATGGTCACCg                                                                                    >  1:492693/1‑210 (MQ=255)                                                                                                                      aCCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGt                                                                               >  1:504207/1‑210 (MQ=255)                                                                                                                                       tttCAACGCCCGCGGTACCGTGCGGGCGAGTGGCGGAGAGGGGCGTAGTTATCAGACCACCAGCCAGGCCCACATTAACAACGGCGAAGGTGGCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGCTGCCGTGGTGAAGGCgg                                                              <  1:156845/210‑1 (MQ=255)                                                                                                                                               cccGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGACGGCGGTTATctgc                                                      >  1:156885/1‑210 (MQ=255)                                                                                                                                                  gcgcTACCTTTCTGGCGACTGGCTGAGCAGGGCGTATTTATCAGTCCACCACCCAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGa                                                   <  2:492724/210‑1 (MQ=255)                                                                                                                                                                      ggCGGACCAGGGCGCATTCATCAGCCCCCCACCAAGGCCCACATTAACACCGGCGACGGTGTCTGCATGGCGATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGtttt                               <  1:430247/210‑1 (MQ=255)                                                                                                                                                                                 gCGTATTTCTCAGTCCACCACCAGTGCCCACATTAACACCGGCGACGGTGTCGGCTTGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTa                    <  1:299519/210‑1 (MQ=255)                                                                                                                                                                                      tttATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATgcgc               <  2:287498/210‑1 (MQ=255)                                                                                                                                                                                       ttATCAGTCCACCCCCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGcc              <  1:78256/210‑1 (MQ=255)                                                                                                                                                                                        tATCAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCg             >  1:527259/1‑210 (MQ=255)                                                                                                                                                                                           cAGTCCACCACCAAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAAc          >  2:322615/1‑210 (MQ=255)                                                                                                                                                                                                   caccaAGGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGa  <  1:165404/210‑1 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                            ACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGA  >  NC_000913/756348‑756751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.