Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA27.9% 167.6 / 15.1 138intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (66/14);  new base A (31/0);  total (124/14)
Fisher's exact test for biased strand distribution p-value = 9.85e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

CGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCA  >  DH1/613620‑613920
                                                                                                                                                      |                                                                                                                                                      
      cccccccacaccccGGCTTACCGGTATTTTTCCCCTGCTTACCGGAAATACCCGCTGGGGCGTGTTTTCGGTTGTGCAGCCGCTGAAAGATCACTGACTCGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGt                                                                                                                                                  <  5:1375683/138‑1 (MQ=2)
                  gCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGGTTCGGCGGGTTTTTGCTTTTTGAGTGGGAGAAATATGAGAGCGTGTCGGCGACACGGTAGTAACAAGACAGCGCCTTAACGGTAAGTTTAAACTTTTGTACGttata                                                                                                                                       >  1:261809/1‑146 (MQ=2)
                    ggCTTATCGGTCAGTTTGACCTGGTTTACGTAAAAACCCGATTCGGCGGGTTTTAGATTTTGGAGGGCCAGAAATATAAACGACTGTCCACGACACGATACGCAAAAGAAAGCGGCGTATCGGTCAGGTTTACGTGAGGTACGTAATAAcc                                                                                                                                    >  2:122795/1‑151 (MQ=255)
                       ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCgt                                                                                                                                 >  3:739083/1‑150 (MQ=18)
                       ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATAAATCTCAACAACAAGATAGACAAAAAAAATGTGGTGAGCTGTCATTTTCACCTTTTTTACGTAAAAAcaaa                                                                                                                                  >  2:509805/1‑147 (MQ=2)
                       ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGAAGAATCACTGTCCACGACACTATACCCAAAAGAAAGCCGCTTATCCCTCAGTTTCACCTGTTTTACGTAAAAAACCgt                                                                                                                                 >  5:974063/1‑150 (MQ=11)
                        tATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTCTCCAAGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTTAACTGATTTACGTAATAACCCggt                                                                                                                                >  7:882929/1‑149 (MQ=11)
                         aTCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGCtt                                                                                                                               >  1:1056527/1‑151 (MQ=25)
                         aTCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTaaaaaaacgatc                                                                                                                               >  8:938042/1‑144 (MQ=255)
                          tCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGTTTTACGTAAAAAACCggtac                                                                                                                              >  3:1189297/1‑147 (MQ=255)
                          tCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCgatac                                                                                                                              >  5:144572/1‑147 (MQ=255)
                          tCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTGTACGTaaaaaaacgatac                                                                                                                              >  7:1191392/1‑143 (MQ=255)
                          tCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTACGGCGGGTTTTTGCTTTTGGAGGGGCAGAAATATAAATCACTGTCCACGACACTAAACCCAACAGCAAGCATCATATCGCTCAGTATAACCTGATTAACGTaaaaaacacgtg                                                                                                                               >  2:798827/1‑143 (MQ=11)
                           cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAGACAAAAGAAATCGGCTGATCTGTCAGTTTACCCTGTTTTAAGTAAAAACCAGCTTcg                                                                                                                             >  3:188269/1‑151 (MQ=11)
                           cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCCGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGGTTcg                                                                                                                             >  5:319389/1‑151 (MQ=255)
                            ggTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATTTACGTAAAAAACCga                                                                                                                                 >  4:225243/1‑145 (MQ=9)
                            ggTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTATCGGAAGAGCACGCTTctg                                                                                                                            >  5:291015/1‑149 (MQ=11)
                               cAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGCTTcggcgg                                                                                                                         >  5:480028/1‑151 (MQ=25)
                                aGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATTTACGTAAAAACCCGATTCGTCggg                                                                                                                        >  4:1277395/1‑151 (MQ=255)
                                aGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGTTCCGGCggg                                                                                                                        >  3:219646/1‑151 (MQ=25)
                                 gTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGGTTCGGCGGGt                                                                                                                       >  8:6556/1‑151 (MQ=255)
                                  tttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAAACCGATCCGGCGGGtt                                                                                                                      >  3:693056/1‑151 (MQ=255)
                                    tCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGCTCCGGCGGGtttt                                                                                                                    >  3:77966/1‑151 (MQ=255)
                                     cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAATAAACCGGTTCGGCGGGtttgt                                                                                                                   >  5:686424/1‑149 (MQ=255)
                                       ccTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGGGtttatc                                                                                                                  >  2:340697/1‑147 (MQ=255)
                                        cTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTGCGGCGGGtttgtgct                                                                                                                >  1:305880/1‑146 (MQ=255)
                                         tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATGTACGTAATAACCCGCTGCGGCGGGTTTATCCtt                                                                                                               >  6:294797/1‑151 (MQ=11)
                                         tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATCACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTAACGGTCAGTTTCACCTGATTTACGTAAAAACCCGATACGGCGGGTTTCAACtt                                                                                                               >  6:288343/1‑151 (MQ=11)
                                          ggTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAACCCGATACGGCGGGTTTTAAAttt                                                                                                              >  2:153825/1‑151 (MQ=11)
                                          ggTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATTCGGCGGGTTTAAACttt                                                                                                              >  8:526862/1‑151 (MQ=255)
                                            tttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGTAGCGGCGGGTTTGTCCttttt                                                                                                            >  7:1250270/1‑150 (MQ=11)
                                            tttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGATACCGCGGGTTTGTACttttt                                                                                                            >  8:570726/1‑150 (MQ=11)
                                             ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTGTGCTTTTgg                                                                                                           >  1:891973/1‑151 (MQ=9)
                                             ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGGTACGGCGGGTTTATACtttttg                                                                                                           >  6:1341435/1‑149 (MQ=11)
                                                  tAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATGCGGCGGGTTTGTGATTTTTGAgtgg                                                                                                      >  8:809239/1‑151 (MQ=11)
                                                    aaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGCAAGCGGCTTATCGGTCAGTTTAACCTGTTGTACGTAATAACCCGATGCGGCGGGTTTTTACTTTTGGAGGGGc                                                                                                     >  7:555877/1‑150 (MQ=11)
                                                         cgcctttttttggtttttggtttttGTGGGGATCAAATATGAATGACTGTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAg                                                                                               <  4:37398/140‑1 (MQ=11)
                                                            cTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCGCTTCGGCGGGTTTCTACTTTTTGAGTGGGAGATAgtct                                                                                            >  5:217631/1‑148 (MQ=11)
                                                             ttCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATGTACGTAAAAACCCGATACGGCGGGTTTAAAATTTTGGAGTGCGAGAAAGaac                                                                                            >  4:1119763/1‑148 (MQ=11)
                                                                ggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTGTACGTAAAAACCCGCTTCGGCGGGTTTGTACTTTTGGAGGGCGAGAAATaggaatg                                                                                        >  7:488844/1‑151 (MQ=11)
                                                                  cggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTTCGGCGGGTTTTTAATTTTTGAGTGCCATAAAGatcaatccc                                                                                      >  1:252499/1‑148 (MQ=11)
                                                                         ttGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGCCAGATAGATCACTCACTGTcccc                                                                               >  7:697117/1‑149 (MQ=11)
                                                                                 ggAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGGGTTTATAATTTTTGAGTGCCATAAATAAAAATCACTGTCCACGacacga                                                                        >  8:340227/1‑148 (MQ=11)
                                                                                          gAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTGTACTTTTGGAGGGGCAGAAAGATGATTGCCTGGCCGCGacacgagacgaaaa                                                                >  1:303688/1‑139 (MQ=11)
                                                                                               atgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAATATGAATGCCTGTCCACGACACTATCCGATAAAGAAAGc                                                         >  5:1188149/1‑151 (MQ=11)
                                                                                               atgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATCCCCAAAAGAAAGc                                                         >  7:1213032/1‑151 (MQ=25)
                                                                                                tgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAAGAAAGACTGTCCACGACACTAAACAAAAAAGACAGCg                                                        >  2:1038246/1‑151 (MQ=11)
                                                                                                      aCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATGCGGCGGGTTTAAAATATTAACAGCGAAGAAAAATGAATGACTGTCAACGCCTGTATACAAATAAGTAGTCGGCTTAt                                                  >  6:825784/1‑151 (MQ=2)
                                                                                                        tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAGTGACTGTCCCCGACACTATACCCAAAAGAAAGCGGCTGATCg                                                >  7:684412/1‑151 (MQ=255)
                                                                                                         gTCGACCACCCTTTACCCAAGTGACCGCTGCCTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCg                                                <  7:1126282/150‑1 (MQ=11)
                                                                                                            cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAAGATTGACTTTCCACGACACAATAGCCAAAAGAAAGCGGCTTATCGGta                                             >  3:125556/1‑149 (MQ=11)
                                                                                                            cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGATACGGCGGGTTTATACTTTTGGAGGGGCATAAAGATGAATCACTGTCGACGACACTAACGCCAACTGAAAGCGGCTTATCGGTc                                             >  4:1223137/1‑150 (MQ=11)
                                                                                                               gACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGCCAGAAATAGGAAAGACTGTCCCCGACACGATACCAAAAAGAAACCGCCATATCGGTCAGtt                                         >  5:1046695/1‑151 (MQ=11)
                                                                                                                   cTATACCCAAAAGAAAGCGGCTTATCGGTCACTTTAACCTGTTTTACGTAAAAACCCGGTACGGCGGGTTTAAAATTTTTGAGGCGCAGAAATAAGAGTCACAGTCCGCGTCACAATACCGAACACGAAGAGCCTTATctggcacagtaac                                     >  5:927367/1‑139 (MQ=2)
                                                                                                                    tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTGTACTTTTGGAGGGGCAGATAGATGTAACACTGTCGACGACACGATCGACAAAAGCAAGCGGCAGATCGGTCAGtgtaac                                     >  6:1386840/1‑145 (MQ=11)
                                                                                                                    tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTGCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGTGTCCCTGGCGCCGACACTGTCCCCAAATGACAGCGGGATCTCGGTCAGtgttcgc                                    >  3:532752/1‑148 (MQ=11)
                                                                                                                    tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTTTACTTTTGGAGTGGCATAAAGATGAATCACTGTCCGCGACACTATAAACAAAAGAAAGCAGCTTATCGCTCAGTATTAc                                     >  8:1190710/1‑150 (MQ=11)
                                                                                                                      taCCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAAGAATGACTGTCCACGAGACAATACCAAAAAGAAAGCGACTTATCGCTCAGTATTACCtt                                  >  6:654655/1‑150 (MQ=11)
                                                                                                                       aCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGGTTCGGCGGGTTTGTACTTTTTGAGGGGCAGAAAGAGGATGCACAGGCCACGACACTAGACACAAATGCAACCGCCATATCGGTCAGTGTTaaattt                                 >  7:807648/1‑146 (MQ=2)
                                                                                                                          cAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGCCAGAAAGAAGATTGCCTTTCCACGACACTATAGCCAAAAGCAAGCGGCTTATCGGTCAGTGTTACCTGAGGt                              >  1:696689/1‑151 (MQ=11)
                                                                                                                          cAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTAATTTTGGAGTGGCAGAAAGAAGAAAGACTGTCCACGACACTATAAACAAAAGAAAGCAGCTTATCGCTCATTGTTACATGATGt                              >  8:1170182/1‑151 (MQ=11)
                                                                                                                           aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTACGGCGGGTTTGTACTTTTGGAGGGCCAGAAAGATGAAACACTGTCCGCGACACTAAACACAAATGAAAGCGGCTTATCGGACAGTATTACCTGATGt                              >  5:641544/1‑150 (MQ=14)
                                                                                                                            aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTGCTTTGTGAGTGGCAGAAAGAGGAGGGGCTGTCCGCGACACTAGCGCCAAAAGAAAGCGGAATATCGGTAAGTGTTAACTGAGGTa                             >  5:1062345/1‑150 (MQ=11)
                                                                                                                             aagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCGCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACg                           >  1:803543/1‑151 (MQ=255)
                                                                                                                              agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTGTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTGTTACCTGATGTACGt                          >  2:643179/1‑151 (MQ=255)
                                                                                                                              agaaagCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTACGGCGGGTTTTTAATTTTGGAGGGGCAGAAATAACAATCACTGTCCACGACACTATAAACAAAAGAAACCGGCTTATCGGTAAGTTTTaaaagatcaa                             >  6:358428/1‑139 (MQ=11)
                                                                                                                               gaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATCACTGTCCACGACACTATAACCAAAAGAAAGCGGCTTATCGGTAAGTTTTAAGTGATGTACGt                          >  5:1229728/1‑150 (MQ=255)
                                                                                                                               gaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTTTTACTTTTGGAGGGGCAGATAGATGAATGACTGTCCCCGACACTAGACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGt                          >  5:614795/1‑150 (MQ=255)
                                                                                                                                aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAACCGGCTTATCGGTCAGTTTTACCTGATGTACGta                         >  1:1082006/1‑150 (MQ=255)
                                                                                                                                 aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                       >  6:13037/1‑151 (MQ=40)
                                                                                                                                 aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAACCAAAAGAAAGCGGCTTATCGGTCATTTTTACATGATGTACGtaat                       >  2:1312442/1‑151 (MQ=255)
                                                                                                                                 aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTAATTTTGGAGGGCCAGAAAGATGAATGACTGTCGACGACACTAAACCCAAAAGAAAGCGGCTTATCGGTAAGTTTTAACTGATCTACGtaat                       >  8:977717/1‑151 (MQ=14)
                                                                                                                                 aagCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAATATGAATCACTTTCCACGACACTAAAAACAAAAGAAAGCGACTTATCGCTAAGTATTAAAAGATGTACGaaat                       >  6:1211104/1‑151 (MQ=11)
                                                                                                                                  agCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                       >  8:148313/1‑150 (MQ=255)
                                                                                                                                    cGGCTTATCGGTCATTTTCACCTTTTTTACTTAAAAATTTGTTTCGGTGTTTTTTTTTTTTTTGAGGTTCATAATTTTTTATTATTTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCgg                                               <  4:1304476/124‑1 (MQ=2)
                                                                                                                                    cGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGCTGCTTATCGGTCAGTTTTACCTGATGTACGtaataa                     >  4:1225072/1‑150 (MQ=255)
                                                                                                                                     ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGaa                                                                                                 <  1:1188250/73‑1 (MQ=38)
                                                                                                                                       catcccctctaGTTTCACCTGATTTCCTTAAAACCCCGCTTCGGCGTTTTTTTTATTTTGGCGGGCCATAAATATTAATCACTGTCCACGACACTATACCCAAAATAAAGCGGCTTATCGGTTAGTTTTACCTGATTTACGTAATAAACCg                 <  4:1117492/141‑1 (MQ=2)
                                                                                                                                       cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                 >  7:79176/1‑151 (MQ=255)
                                                                                                                                       cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAACg                 >  2:280683/1‑151 (MQ=255)
                                                                                                                                        ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt                >  8:607040/1‑151 (MQ=255)
                                                                                                                                         tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt               >  2:1120052/1‑151 (MQ=255)
                                                                                                                                         tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt               >  3:255206/1‑151 (MQ=255)
                                                                                                                                         atggggTCAGTTTCACCTGGTTTACGTAAAACCCCGGTTCGGCGGTTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt                <  3:225098/146‑1 (MQ=14)
                                                                                                                                          accgatCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTTCTTTTGGAGGGCCACAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTc              <  3:1169222/146‑1 (MQ=255)
                                                                                                                                          aTCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTATCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATGATGTACGTAATAAACCGATc              >  2:364888/1‑151 (MQ=255)
                                                                                                                                            cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg            >  6:146297/1‑151 (MQ=255)
                                                                                                                                            cGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTATGCTTTTGGAGGGGCAGAAATATTAATGACTGTCCACGACACTATACCCAAAAAAAAGCGGCTTATAGGTCAGTATTAACTGATATACGTAAAAAAACgatacg            >  2:950813/1‑146 (MQ=11)
                                                                                                                                             ggTCAGTTTCAGCTGTTTTACGTAAAAACCCGATACGGCGGGTTTATACTTTTGGAGGGGCAGAAAGATCAATGACTGTCCACGACACTATACCCAAAAGAAAGCGCCTTATCGGTCAGTTTTAACTCAAGTACGTAATAAAAAGTTAcgg           >  6:1251437/1‑151 (MQ=14)
                                                                                                                                             ggTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg           >  6:1157985/1‑151 (MQ=255)
                                                                                                                                               tCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg         >  8:431700/1‑151 (MQ=255)
                                                                                                                                               tCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCTGTCAGTTTTACCTGATGTACGTAAAAAACCGTTCcggcg         >  1:752466/1‑151 (MQ=255)
                                                                                                                                                 aGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg       >  3:1126024/1‑151 (MQ=255)
                                                                                                                                                 aGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg       >  3:531042/1‑151 (MQ=255)
                                                                                                                                                 aGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg       >  5:410004/1‑151 (MQ=255)
                                                                                                                                                   tttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt     >  6:51642/1‑151 (MQ=255)
                                                                                                                                                     tCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATCATGTACGTAATAAAACGTTCCGGCGGGTTTc   >  6:370342/1‑151 (MQ=255)
                                                                                                                                                      cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAATATTAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCa  <  2:752062/151‑1 (MQ=255)
                                                                                                                                                      cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc   >  8:1290941/1‑150 (MQ=255)
                                                                                                                                                      cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc   >  4:174256/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCA  >  DH1/613620‑613920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑