Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.34.6% 230.4 / 137.5 112intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (61/7);  new base . (34/2);  total (99/13)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-02

Only 100 of 117 total aligned reads displayed.

GTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936192‑936443
                                                                                                       |                                                                                                                                                      
gTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGGAGTGCGTGAATTCTGCACTATATAATTGGTTGCGGGGGGAGTTTTTGACCCGCCGCCATTCGGGttctt                                                                                                       >  5:619944/1‑148 (MQ=2)
                ttGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTAATCGATGTATTCTTCGATGTATTTTTCATTGCGCGGGAGGGATTAGCACCGACGCCCTTAGGGTTATTAGTCCGACGAGCTaa                                                                                        >  8:1205133/1‑149 (MQ=2)
                            ccTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCAGCTATGTTTAATTGGTTGCGGCGGCCGGTTTTGAACCGACGCCCTTCGGGTTATGATCCCGCCGAGAGACCAGGCTGCTcccc                                                                           >  7:81622/1‑149 (MQ=11)
                                              gacgaGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAGTCCGTGACTTCTGCTATGTTTATTTGGTTGCGCGGGGGGGATTTGACCCGCCCCGCTTGGGGTTATGATCCCGACGAGCTACAAGGCTGCTCCCCCGCGCGTcgggcgatg                                                           >  1:111190/1‑141 (MQ=2)
                                                       ccAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACt                                                >  3:432076/1‑151 (MQ=255)
                                                           gctgctCCACCCCGCGTCACCGTAATGCGTTACTTCATCAAATTTTAATTGGTTGCGGGGGCAGGATTTGACCCGACGACATTCGGGTTATGAGCCCGAAGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATTCGCACTATACTCCGc                                            >  4:281375/1‑151 (MQ=17)
                                                             tgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACCAGCTACCAGCCTGCTCCACACCGAGTCCGTGGATGCGCACTATACTCCGCTc                                          >  4:979352/1‑151 (MQ=255)
                                                              gctCCACCCCGCGTCACCGTAGTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCAGGATTTGAACCGACCACCTTCGGGTTATTATACCGACGAGCTACCAGGCTGCTCCCCACGTAGTCGGTTGATGAGCACTATACTCCGCt                                           >  4:1162486/1‑149 (MQ=17)
                                                               ctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgc                                        >  5:892979/1‑151 (MQ=255)
                                                                tCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGACGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCTGATCCACCCCGCGTCAGCGGATGCGCACAATTCTGCCCTcg                                         >  1:875796/1‑149 (MQ=11)
                                                                tCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGACCGACGCGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                       >  2:1285667/1‑151 (MQ=255)
                                                                 ccACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                      >  2:416946/1‑151 (MQ=255)
                                                                  cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGAAGAGCTACCAGGCTGCTCCACACCGCGTCAGTGGATGCGCACTATACTCCGCTCGCGtt                                     >  1:613916/1‑151 (MQ=255)
                                                                    ccccctctCACCGTAGTGCTTTACCTCCTGAAAATTTTATTGGTTGCGGGGGCAGGAGTTGAACCGACGACCTTCGGGTTATGAGCACGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTTGATGCTCACTATACTCCGCTGGCGtt                                     <  3:1341430/142‑1 (MQ=17)
                                                                    ccccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACAGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                                   >  6:389940/1‑151 (MQ=255)
                                                                     cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  6:1289240/1‑151 (MQ=255)
                                                                       cgcgTCCCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGACCCGCCGCCCTCCGGGTTAGGAGCCCGCCGCGCTCCCAGGCGGAGCCCCCCCGCGGCCGGGGATGCGCCCTATCCTCCGCTCGCGtttgc                                  >  1:517811/1‑147 (MQ=18)
                                                                       cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAATCGCAGTATACTACTCACGCGTTTTCAt                                >  1:17268/1‑151 (MQ=1)
                                                                       cgcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                                >  7:1257989/1‑151 (MQ=255)
                                                                        gcttCACGGTACTGCTTTTACTTCATAAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                               <  2:17201/148‑1 (MQ=14)
                                                                        gcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATTAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGGAATCCCAGTATACTccaaaagatatttaatt                               >  6:567751/1‑136 (MQ=14)
                                                                        gcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCCCt                                           >  1:49065/1‑139 (MQ=11)
                                                                          gTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAGGCGCACAATTCTACCCTAGAGTTTTCAttc                              >  6:363678/1‑148 (MQ=11)
                                                                          gTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  4:136888/1‑150 (MQ=255)
                                                                           tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAAGCGGACTATACAACGCACGAGTTttcattca                             >  4:1211902/1‑150 (MQ=11)
                                                                           tCACCGTACTGCTTTTACTTCATAAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACtat                                                   <  4:560530/128‑1 (MQ=11)
                                                                           tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  5:989183/1‑150 (MQ=255)
                                                                           tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  1:688640/1‑150 (MQ=255)
                                                                           tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  8:363850/1‑150 (MQ=255)
                                                                            cACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAAGCGAAATATACAACGATCGATATTTCATTCAAc                           >  6:1203914/1‑151 (MQ=11)
                                                                              ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGCGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGTACTATACACCGCTGGCGTTTTccttgcggcg                         >  8:1080697/1‑142 (MQ=11)
                                                                              ccGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                         >  4:587960/1‑151 (MQ=255)
                                                                              ccGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                         >  2:1360667/1‑151 (MQ=255)
                                                                               cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATTCGGACTATACTCCGATAGATTTTTCAt                                >  7:1236732/1‑143 (MQ=1)
                                                                               cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGCGTTTTCATggcgccgt                        >  5:433251/1‑144 (MQ=14)
                                                                               cGTACTGCTTTTACTTAATAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                                <  8:1236059/143‑1 (MQ=17)
                                                                               cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                        >  6:915438/1‑151 (MQ=255)
                                                                               cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                        >  7:630878/1‑151 (MQ=255)
                                                                                gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                       >  5:1344208/1‑151 (MQ=255)
                                                                                 tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  6:742809/1‑151 (MQ=255)
                                                                                 tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  1:1070271/1‑151 (MQ=255)
                                                                                 tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGCATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  5:1111601/1‑151 (MQ=255)
                                                                                  aCTGCTTTTAATAATAAAATTTTTATTGGTTGCGGTGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                     <  5:568273/151‑1 (MQ=21)
                                                                                   cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    >  6:1351297/1‑151 (MQ=255)
                                                                                   cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    >  8:403161/1‑151 (MQ=255)
                                                                                   acttcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATCGGGTTTTCATGCAACCgtttt                     >  5:1103557/7‑151 (MQ=17)
                                                                                    tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTAATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCCTCCGTGGATGCGCACTATACTCCGCTCGCCTTTTCATGCAACCtttttgt                   >  4:601768/1‑149 (MQ=255)
                                                                                    tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGAACTTCGGGTTATGAGCCCGACCAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGGTTTCATGCAACCttttttt                   >  8:662269/1‑151 (MQ=255)
                                                                                    ctgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAAGCGCACAATACAACACATGATTTTTCATGCAACCgttttt                    >  8:1068695/6‑151 (MQ=11)
                                                                                    ctgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAAGCGCACAATACTACGCTGGAGTTTTCATGCAACCtttttt                    >  4:104377/6‑151 (MQ=14)
                                                                                    ctgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGGAAGCCCAAAATACACCACAAGAGAATTCATTCAACCtttttt                    >  4:969638/6‑151 (MQ=14)
                                                                                    accgatcaaCTTCATAAAATTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    <  8:480237/143‑1 (MQ=18)
                                                                                     tgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAGTATACTCCGATCGAGTTTTCATTCAGCGttttttt                   >  5:936495/5‑151 (MQ=14)
                                                                                      gcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGACAATACTCCCATCGAGTTTTCATGCACCCgttttttt                  >  7:435896/4‑151 (MQ=14)
                                                                                      gcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCGCACTCTACTCCGCTAGCGTTTTCATGCAACCtttttttt                  >  5:144386/4‑151 (MQ=17)
                                                                                      gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                  >  3:45453/1‑151 (MQ=255)
                                                                                      gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                  >  8:1329139/1‑151 (MQ=255)
                                                                                      gCTTTACCTAATTAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACGTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   <  3:155854/150‑1 (MQ=255)
                                                                                       cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                  >  7:496599/1‑150 (MQ=255)
                                                                                        ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTCCCATCTGGTTTTCATTCCACCGTTTTTTTa                 >  5:965055/2‑150 (MQ=14)
                                                                                        ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTAGCGTTTTCATGCACCCTTTTTTTTAc                >  6:788570/2‑151 (MQ=255)
                                                                                        ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGAACTATACTCCCAACGCGTTTTCATGGAACCtgagtttta                 >  4:1252695/2‑142 (MQ=14)
                                                                                        ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCGCACTATACTCCGCAGGGGTTTTCTTGGACCCtgactttta                 >  7:1077989/2‑142 (MQ=11)
                                                                                         tttCCTTCTGCAAATTTGATTGGTTGGGGCGGCCGGATTTTAACCGAGGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACGCGGCGTGCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                  <  3:47132/148‑1 (MQ=18)
                                                                                         tatACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCCTCCGTGGATGCGCACTATACTCCGCTCGCCTTTTCATGCAACCTTTATTTTAc                >  6:1321636/3‑151 (MQ=255)
                                                                                         taagatctttcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTa                 <  1:191225/140‑1 (MQ=18)
                                                                                          ttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCCATTATACTGCGCTAGATTTTTCATGCAACCTTTTTTTTAccc              >  3:522370/1‑151 (MQ=17)
                                                                                            ccgagcttaaaaTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              <  6:634582/142‑1 (MQ=255)
                                                                                            aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc             >  1:32100/1‑151 (MQ=255)
                                                                                            aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc             >  3:1061677/1‑151 (MQ=255)
                                                                                            aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc             >  7:1095615/1‑151 (MQ=255)
                                                                                            aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTACGATCGCGTTTTCATTCACCCttttttagatccg             >  7:480462/1‑143 (MQ=17)
                                                                                            aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTACCTGGATGCCGATTATACTGCGCTGGAGTTTTCATGCACCCtgaattatacccac            >  5:1358769/1‑138 (MQ=11)
                                                                                             cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            >  5:785677/1‑151 (MQ=255)
                                                                                             cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            >  7:153763/1‑151 (MQ=255)
                                                                                             cTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGAATATACAGCACTCTCTATTTAATGCACCCTTAAGTTTACCACg            >  3:698171/1‑150 (MQ=11)
                                                                                              ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg            >  4:824304/1‑150 (MQ=255)
                                                                                               tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  8:1101225/1‑151 (MQ=255)
                                                                                               tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  5:1138824/1‑151 (MQ=255)
                                                                                               tcatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGTATGCCGAGAATACTACGCAATGGTATTCATTCAACCtttttttttacaccatc         >  5:278674/1‑142 (MQ=11)
                                                                                                ccataaaTTTTGATTGGTTGCGGGGGCCGTATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc         <  1:1037630/147‑1 (MQ=255)
                                                                                                catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc         >  3:1176930/1‑151 (MQ=255)
                                                                                                catcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCTGTATACTGCGCTCGCGTTTTCATGCACCCTTTTTTTTACCCCGATc         >  5:193191/1‑150 (MQ=18)
                                                                                                 atcaAATTTTAATTGGTTGCGGGGGACGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTGTTTTAACCCCGATc         >  2:1290452/1‑150 (MQ=255)
                                                                                                 atcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc       >  5:75622/1‑151 (MQ=255)
                                                                                                 atcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGACCTACCAGGCTGCTCCACCCCGCGTCCGTGGAAGCGCACAATACACCGATCGCGTTTTCATGCAACCTTATTtata                 >  2:191433/1‑141 (MQ=17)
                                                                                                  tcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGACTATACTACGCTCGCGTTTTCCTGCACCCTTTGTTTTAaccagctcgg       >  5:635028/1‑143 (MQ=17)
                                                                                                   caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg      >  6:638660/1‑151 (MQ=255)
                                                                                                    aaaaTTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     <  3:104109/151‑1 (MQ=255)
                                                                                                    aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  7:64523/1‑151 (MQ=255)
                                                                                                    aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  6:592969/1‑151 (MQ=255)
                                                                                                    aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTACCCCGATCACgg     >  2:1020987/1‑150 (MQ=255)
                                                                                                    aaaTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGa    <  6:964158/151‑1 (MQ=255)
                                                                                                     aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGCCGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  5:143307/1‑150 (MQ=255)
                                                                                                     aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  3:357885/1‑150 (MQ=255)
                                                                                                      aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGCCGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt   >  6:1074244/1‑151 (MQ=255)
                                                                                                      aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt   >  3:506725/1‑151 (MQ=255)
                                                                                                       ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGCTCACGGAtt  >  4:1311109/1‑151 (MQ=255)
                                                                                                       ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt  >  3:1058992/1‑151 (MQ=255)
                                                                                                       ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt  >  5:828886/1‑151 (MQ=255)
                                                                                                       |                                                                                                                                                      
GTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936192‑936443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: