BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	1,065,605	0	A	T	31.6%	171.7 / 76.8	117	intergenic (+40/‑23)	ECDH1_RS05245/ECDH1_RS05250	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base A (54/26); new base T (23/14); total (77/40)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065457‑1065754

       tCTGCTGTAATGTAAGGTTCCCGTTGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTaaa                                                                                                                                               <  3:908307/150‑1 (MQ=255)

        attcattaaagtaaGTTACCCGACCCATCCGTAGCTCAGCTCCATACAGTACTCCGCTAAGCAC‑CCACATCCCCTATCGACGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAaaat                                                                                                                                             <  8:295901/145‑1 (MQ=9)

                    atacctccctttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGAt                                                                                                                                 <  7:470083/140‑1 (MQ=255)

                     agttgcgccgtGCAGCCGTAGCTTAACTGGAGTTAATACTCGGCCACTACC‑GGTGGGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATg                                                                                                                                <  1:689976/141‑1 (MQ=17)

                      accgccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTTAATATCACATAAGAAGCTATATA‑‑AAA‑‑ATAAGCAGTAAAAAAAAATTccacaa                                                                                                                                 >  8:469886/10‑145 (MQ=2)

                        cgccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAATACTTAATATGGCATTAGTAGCGATATA‑‑AAT‑‑ATAAGCAGTaaaaaaaa                                                                                                                                          >  2:873890/8‑138 (MQ=14)

                            cGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAATACGAGATATGCATGAAGTATCGTAAGC‑‑AAG‑‑ATCTCCAGTAAATAAAATAACCCGATACagacg                                                                                                                          >  8:283118/1‑146 (MQ=2)

                                gCATCGGTAGCACAGCTGGATTGACTACTCGGCTACTATCGCGTG‑GGTCGGCGCTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAg                                                                                                                       <  7:648546/149‑1 (MQ=17)

                                 cATCCGTAGCTCAGCTGGAGAGAGTACTCGGCTACGACC‑GGAGCGGTCGGAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTc                                                                                                                    <  7:352338/151‑1 (MQ=255)

                                     cGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTAGATCTGGAGAAATGAGCGGTACC‑‑AAG‑‑AACACCAGGAAATAAAATTACCCAATACAtaagtaaaacatcc                                                                                                                >  3:1279277/1‑138 (MQ=0)

                                      gTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTAGATATTCCTAAATTAACGGAAAC‑‑AAT‑‑ATCATCAGTAATATAAATATCGCTATACATACGAAgaagatcat                                                                                                               >  4:1360726/1‑143 (MQ=9)

                                          gtCAGCTGGATTTTCTACTCGGCGACGAGCGCTAG‑GCTCGGAGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATa                                                                                                           <  1:386855/150‑1 (MQ=14)

                                           tCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACTAGATATGGCGGTATGAGCGGTTGG‑‑AAG‑‑ATCTTGAGTAATTTAAATTTCGCGCTTCGTACGTATCTCATCTGAAt                                                                                                            >  7:1062886/1‑149 (MQ=2)

                                                tGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTTATATGGCTTTATTAGCGGTAGC‑‑AAT‑‑ATCACCAGTAAAATAAATTTCCCGATGCATACGTAGCTCAGcgttatacagaac                                                                                                     >  5:160650/1‑139 (MQ=21)

                                                                       c‑cGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTtcc                                                                                                                                    >  1:833238/1‑97 (MQ=255)

                                                                             cGGTCGGCGCGTCGAATCCGCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGGCGGAGGtt                                                                         <  6:756291/151‑1 (MQ=255)

                                                                              ggTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTgccc                                                                                                                                   >  1:591989/1‑91 (MQ=255)

                                                                               gTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTa                                                                                                                                                 >  5:1256699/1‑77 (MQ=255)

                                                                                   gcgcgtctaaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGaa                                                                     <  1:17242/141‑1 (MQ=255)

                                                                                    ggtgtctaaTCCTCCGTTATTCAACATCTCTTACTATATATGGCTTTACTAGCTTTATA‑‑AAT‑‑ATCAGCAGTAAACTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcc                                                                  <  4:136572/144‑1 (MQ=14)

                                                                                        tCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATcagcag                                                                                                                                                   >  2:388349/1‑66 (MQ=39)

                                                                                        tCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTag                                                                                                                                                >  6:499389/1‑68 (MQ=38)

                                                                                         cGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTGTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGATCCGAGCGGTCGGAGGTTCGAATCCTCCCg                                                             >  6:1058309/1‑151 (MQ=255)

                                                                                          gAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTagaa                                                                                                                                              >  3:283434/1‑69 (MQ=14)

                                                                                           aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCggg                                                           >  8:842288/1‑150 (MQ=39)

                                                                                             tcctccCGGATGCACCATCTATTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGATCAGCTGGATAGAGTACTCGGATACGAACCGAGCGGTCGGAGGTTCGAATACTCCCGGATg                                                         >  2:892371/1‑151 (MQ=255)

                                                                                              cgagctctctacaacATCTCTTAGTTGATTTCTCTTTAGTAGGTCTATC‑‑AAT‑‑ATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGc                                                        <  1:292179/137‑1 (MQ=11)

                                                                                                   cGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat                                                   >  4:1097467/1‑151 (MQ=39)

                                                                                                   cGGATGCACCATCTCTTAATTGATATGGGTTTATTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCAGATGCATCCGTAGCTCAGATGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat                                                   >  2:592164/1‑151 (MQ=255)

                                                                                                      aTGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATAtt                                                >  3:514837/1‑151 (MQ=39)

                                                                                                        gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATca                                                                                                                                                       >  4:639478/1‑46 (MQ=35)

                                                                                                        gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTaga                                                                                                                                               <  4:52842/54‑3 (MQ=14)

                                                                                                        gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTaga                                                                                                                                               >  3:52804/1‑52 (MQ=14)

                                                                                                        gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                              >  6:785597/1‑151 (MQ=39)

                                                                                                        gCACCATCTCTTACTTGATATGGCTTTAGTAGAGGTATC‑‑AAT‑‑ATGAGCAGTAAATTAAATTTAACGATGCATACTTAGCTCAGCTGGATAGAGTAATCGGCTACGAGCCGAGCGGTCGGAGGTTCGAATCCTCCCGGCTggcgcagattcg                                              >  3:876601/1‑140 (MQ=14)

                                                                                                             aTCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGa                                          >  5:594315/1‑150 (MQ=40)

                                                                                                              tctcTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGaa                                         >  7:891776/1‑150 (MQ=255)

                                                                                                               ctctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAt                                       >  8:59899/1‑151 (MQ=255)

                                                                                                                  ttACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGt                                                                                                                                                  >  1:182572/1‑41 (MQ=35)

                                                                                                                  ttACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGTAAACtt                                    >  1:717798/1‑151 (MQ=255)

                                                                                                                   tACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAAACtt                                    >  2:376052/1‑150 (MQ=255)

                                                                                                                       tGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGc                                >  6:543420/1‑150 (MQ=255)

                                                                                                                        gATATGGCTTTACTAGCTGTATA‑‑AAT‑‑ATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCa                               <  7:988770/150‑1 (MQ=11)

                                                                                                                         atatGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAAt                             >  3:337561/1‑151 (MQ=255)

                                                                                                                         atacGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATGCAga                                >  2:196879/1‑149 (MQ=14)

                                                                                                                          tatGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTAATCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGCAATg                            >  2:1043845/1‑151 (MQ=255)

                                                                                                                             ggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTCCTAAATATTcctttatt                            >  3:649815/1‑143 (MQ=14)

                                                                                                                             ggCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGaa                          <  6:593860/150‑1 (MQ=255)

                                                                                                                             ggCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                           >  7:867649/1‑149 (MQ=255)

                                                                                                                               cTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTACTACGAAATATTCCTCAATGaac                         >  5:927649/1‑150 (MQ=14)

                                                                                                                               cTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGAACCTCAGCAGGATAGAGTACTCGGCTACCAACCGACCGCTCCGAGGTTCCAATCCTCCCCCATGCACCAAATTAAACCAAAACATCAGCAAAGAAgg                        >  4:901406/1‑150 (MQ=14)

                                                                                                                               cTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGATATATCAAAACATTACCGATGCATACGTATCTCTGCTTGAAATAGACATAGCCTTCTAACCGAGGTATAATAAGTTCGAATCGGCCCGGTTGCCCCATATTCTGGGAATTCTTGCGCAATGAAGGt                       >  3:37486/1‑151 (MQ=255)

                                                                                                                                ttttataggtttata‑‑AAT‑‑ATCTGCAGTAAACTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                       <  2:1060303/139‑1 (MQ=11)

                                                                                                                                tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAGATCtgccggaaggatgg                        >  8:666502/1‑138 (MQ=11)

                                                                                                                                tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGCAATGATgg                        >  5:847616/1‑151 (MQ=11)

                                                                                                                                tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGAAATCTTCAGCAATGAAgg                        >  4:1178474/1‑151 (MQ=11)

                                                                                                                                 ttagtgtggaaaTC‑‑AAT‑‑ATCTCGAGTAATCTAAAATTCGCTATCCATCCGTAGCTCAGCTTGATAGAGTACTCGGCTACGAACCGCGGGGTCTGAGCTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTa                      <  7:229948/139‑1 (MQ=14)

                                                                                                                                 ttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGAAATCTTCAGCAATGAAGGt                       >  6:723477/1‑151 (MQ=11)

                                                                                                                                 ttAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                     >  8:705530/1‑151 (MQ=255)

                                                                                                                                  tagtagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGa                                                                             >  7:876316/1‑94 (MQ=255)

                                                                                                                                  tagtagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                     >  6:1367965/1‑150 (MQ=255)

                                                                                                                                  tagcatcGGAATG‑‑AAT‑‑ATCTGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                     <  1:463630/144‑1 (MQ=14)

                                                                                                                                   agtagATGTATC‑‑TAT‑‑TTCAGTAGTATAATATCTTTCGATCTGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCg                   <  8:1294413/151‑1 (MQ=11)

                                                                                                                                    ataggtatata‑‑AAA‑‑ATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCg                   <  4:649570/143‑1 (MQ=11)

                                                                                                                                     tagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCACGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                  >  4:1338974/1‑150 (MQ=255)

                                                                                                                                      aggtctctC‑‑AAT‑‑ATCAGCAGTAAAATAATTGTCCCGATGCATCTTTAGCTCAGCTGGATATAGTACTCGGCTACGATGGGTTGGGGCGGTGGTTGTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                 <  8:35269/143‑1 (MQ=11)

                                                                                                                                      agCGTTATA‑‑AAT‑‑ATCTGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                 <  5:625604/150‑1 (MQ=255)

                                                                                                                                      agCGGTCTA‑‑AAT‑‑ATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                <  7:508710/151‑1 (MQ=255)

                                                                                                                                      agCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCGAAATATTCAGCTATGAAGGTAtgc                   >  6:735246/1‑148 (MQ=11)

                                                                                                                                      agCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCGATGCATGCGTAGCTCAGCTGAATAGAGTACTGTGGGACGAACCGATCTGTCCGAGGTTCGAATACTGCCGGATGCAAGATATTTTAATAAATATTCGGCAGTGAGGGtgtgga                  >  4:214924/1‑144 (MQ=9)

                                                                                                                                      agCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCGATGCATCCGTAACTCAGCTGAATAGCGTCCTGGGGGACGAACCGAGCGGTCCGAGGTTCGAATACTGGCGGATCCCACATATTAAAAGAAATCAGCGGCTATGAAGGGGTCGa                  >  4:221661/1‑149 (MQ=2)

                                                                                                                                       gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTTTCCGAAATCTTCATAAATGATCGTAtaca                  >  1:147970/1‑147 (MQ=11)

                                                                                                                                       gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGATATCTTCATGAATGAAGGtttgga                  >  5:1256227/1‑145 (MQ=11)

                                                                                                                                       gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGCTCCGAGATATGCAgagaacagcgctcaaa                  >  1:422479/1‑135 (MQ=14)

                                                                                                                                          ttata‑‑AAT‑‑ATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAgg             <  6:695207/149‑1 (MQ=255)

                                                                                                                                           tatcaaaaT‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGaa                          <  3:618166/132‑1 (MQ=14)

                                                                                                                                           tATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAGCGAGCGGTCGGAGGTTCGAATCCTCCCGGAAGCACCATATTCTCCGACATCTTCAGCAATGAAGGTATCGATGAGGtg           >  3:647542/1‑150 (MQ=255)

                                                                                                                                           tATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATCCATCCGTAGCTCAGCTGGATAGAGTACTCGCCTACGAACCGACCGCTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagagct            >  7:5877/1‑148 (MQ=255)

                                                                                                                                            gtatcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagag              <  1:796956/141‑1 (MQ=11)

                                                                                                                                            gatgttaaaaatCTGCAGTAAAGTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagag              <  5:1336231/141‑1 (MQ=11)

                                                                                                                                            aTC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAg          <  7:59994/151‑1 (MQ=255)

                                                                                                                                              c‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg        >  2:545024/1‑151 (MQ=255)

                                                                                                                                                ataaaaatCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTa           <  3:952566/144‑1 (MQ=255)

                                                                                                                                                aaaT‑‑ATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg        <  2:904840/150‑1 (MQ=255)

                                                                                                                                                 aaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAGATCTTCAGGAATGAAGGGATGGAagagagagc         >  6:1335760/6‑146 (MQ=11)

                                                                                                                                                 aaaaaatCAGCAGTAAACTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGc         <  3:553803/146‑1 (MQ=255)

                                                                                                                                                 aaT‑‑ATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg       <  6:107806/151‑1 (MQ=255)

                                                                                                                                                 aaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGATGAGGTAGCgg       >  4:215840/1‑151 (MQ=255)

                                                                                                                                                 aaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg       >  3:119300/1‑151 (MQ=255)

                                                                                                                                                 aaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg       >  4:28133/1‑151 (MQ=255)

                                                                                                                                                  at‑‑atCAGCAGTAAAATAAATTTCCAGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGa      <  1:892046/151‑1 (MQ=255)

                                                                                                                                                   t‑‑atCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGa      <  2:290964/150‑1 (MQ=255)

                                                                                                                                                   t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                    <  5:1003891/88‑1 (MQ=255)

                                                                                                                                                   t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                    >  6:1002914/1‑88 (MQ=255)

                                                                                                                                                   t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCACCAATGAAGGTATCGAAGAGGTAGCGGa      >  7:33271/1‑150 (MQ=255)

                                                                                                                                                   aaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACGAAATATTCAGAAATGAACGTATAGataataagaacg       >  2:463657/4‑140 (MQ=1)

                                                                                                                                                    aaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAGATCTTCAGCAATGAAGGTATGGAagagctcgagg       >  5:1166363/3‑143 (MQ=11)

                                                                                                                                                     aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATCTTCATCAATGAAGGGATGGAAGATCTAGCGGa      >  3:861164/2‑150 (MQ=14)

                                                                                                                                                     aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATCTTCAGCAATGAAGGTATCGAAGATGTAGCGGa      >  1:291092/2‑150 (MQ=14)

                                                                                                                                                      atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAGCTTCAGCAATGAAGGTATCGAAGAGATAGCGGTAt    >  5:616894/1‑151 (MQ=255)

CAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065457‑1065754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`