Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6140AG28.7% 171.7 / 80.1 122intergenic (+49/‑14)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (50/22);  new base G (23/6);  total (78/44)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 139 total aligned reads displayed.

CTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGC  >  DH1/1065465‑1065763
                                                                                                                                                          |                                                                                                                                                     
attcattaaagtaaGTTACCCGACCCATCCGTAGCTCAGCTCCATACAGTACTCCGCTAAGCAC‑CCACATCCCCTATCGACGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAaaat                                                                                                                                                      <  8:295901/145‑1 (MQ=9)
            atacctccctttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGAt                                                                                                                                          <  7:470083/140‑1 (MQ=255)
              accgccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTTAATATCACATAAGAAGCTATATA‑‑AAA‑‑ATAAGCAGTAAAAAAAAATTccacaa                                                                                                                                          >  8:469886/10‑145 (MQ=2)
                    cGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAATACGAGATATGCATGAAGTATCGTAAGC‑‑AAG‑‑ATCTCCAGTAAATAAAATAACCCGATACagacg                                                                                                                                   >  8:283118/1‑146 (MQ=2)
                        gatttcttAGGTCAGCTGTTGAGATTAATCGGCTACGATCGCTAG‑GGTCGTCGCGTCTAATCCTCCCTGATTCACCATCTCTTACTTGATATGGCTTTAGTAGCGTTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCt                                                                                                                              <  7:403235/144‑1 (MQ=9)
                        gCATCGGTAGCACAGCTGGATTGACTACTCGGCTACTATCGCGTG‑GGTCGGCGCTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAg                                                                                                                                <  7:648546/149‑1 (MQ=17)
                             cGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTAGATCTGGAGAAATGAGCGGTACC‑‑AAG‑‑AACACCAGGAAATAAAATTACCCAATACAtaagtaaaacatcc                                                                                                                         >  3:1279277/1‑138 (MQ=0)
                              gTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTAGATATTCCTAAATTAACGGAAAC‑‑AAT‑‑ATCATCAGTAATATAAATATCGCTATACATACGAAgaagatcat                                                                                                                        >  4:1360726/1‑143 (MQ=9)
                                  gtCAGCTGGATTTTCTACTCGGCGACGAGCGCTAG‑GCTCGGAGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATa                                                                                                                    <  1:386855/150‑1 (MQ=14)
                                   tCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACTAGATATGGCGGTATGAGCGGTTGG‑‑AAG‑‑ATCTTGAGTAATTTAAATTTCGCGCTTCGTACGTATCTCATCTGAAt                                                                                                                     >  7:1062886/1‑149 (MQ=2)
                                        tGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTTATATGGCTTTATTAGCGGTAGC‑‑AAT‑‑ATCACCAGTAAAATAAATTTCCCGATGCATACGTAGCTCAGcgttatacagaac                                                                                                              >  5:160650/1‑139 (MQ=21)
                                        tGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGAGATATTGAGGTATTATCGTTTAC‑‑AAT‑‑ATCTAGAGTAATATAAATGTCCCGATACATAAGTAACTAAGCTGGATAGAGTAc                                                                                                              >  6:6907/1‑151 (MQ=9)
                                        ggctttttctACTCGGCGACGATC‑GCGCCGCTCGGAGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTAc                                                                                                              <  5:411668/142‑1 (MQ=14)
                                                               c‑cGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTtcc                                                                                                                                             >  1:833238/1‑97 (MQ=255)
                                                                     cGGTCGGCGCGTCGAATCCGCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGGCGGAGGtt                                                                                  <  6:756291/151‑1 (MQ=255)
                                                                      ggTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTgccc                                                                                                                                            >  1:591989/1‑91 (MQ=255)
                                                                          ggcgagtaataTCCTCCCGTATTCACGATCTATTTTTTTATCTGGCTAGACTAGCGTTATA‑‑AAA‑‑ATCAGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                             <  1:350385/141‑1 (MQ=2)
                                                                           gcgcgtctaaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGaa                                                                              <  1:17242/141‑1 (MQ=255)
                                                                            ggtgtctaaTCCTCCGTTATTCAACATCTCTTACTATATATGGCTTTACTAGCTTTATA‑‑AAT‑‑ATCAGCAGTAAACTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcc                                                                           <  4:136572/144‑1 (MQ=14)
                                                                                  gAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTagaa                                                                                                                                                       >  3:283434/1‑69 (MQ=14)
                                                                                   aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCggg                                                                    >  8:842288/1‑150 (MQ=39)
                                                                                   aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAGC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCACGATGCATCCGTAGCTCAGGTGGATAGAGTACTCGGCTACGAACCGCGCGGTCGGAGGTTCGAATCCTCCCgg                                                                     >  1:70408/1‑150 (MQ=255)
                                                                                     tcctccCGGATGCACCATCTATTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGATCAGCTGGATAGAGTACTCGGATACGAACCGAGCGGTCGGAGGTTCGAATACTCCCGGATg                                                                  >  2:892371/1‑151 (MQ=255)
                                                                                      cgagctctctacaacATCTCTTAGTTGATTTCTCTTTAGTAGGTCTATC‑‑AAT‑‑ATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGc                                                                 <  1:292179/137‑1 (MQ=11)
                                                                                           cGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat                                                            >  4:1097467/1‑151 (MQ=39)
                                                                                           cGGATGCACCATCTCTTAATTGATATGGGTTTATTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCAGATGCATCCGTAGCTCAGATGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat                                                            >  2:592164/1‑151 (MQ=255)
                                                                                              aTGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATAtt                                                         >  3:514837/1‑151 (MQ=39)
                                                                                                gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                                       >  6:785597/1‑151 (MQ=39)
                                                                                                gCACCATCTCTTACTTGATATGGCTTTAGTAGAGGTATC‑‑AAT‑‑ATGAGCAGTAAATTAAATTTAACGATGCATACTTAGCTCAGCTGGATAGAGTAATCGGCTACGAGCCGAGCGGTCGGAGGTTCGAATCCTCCCGGCTggcgcagattcg                                                       >  3:876601/1‑140 (MQ=14)
                                                                                                       ctctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAt                                                >  8:59899/1‑151 (MQ=255)
                                                                                                          ttACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGTAAACtt                                             >  1:717798/1‑151 (MQ=255)
                                                                                                               tGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGc                                         >  6:543420/1‑150 (MQ=255)
                                                                                                                gATATGGCTTTACTAGCTGTATA‑‑AAT‑‑ATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCa                                        <  7:988770/150‑1 (MQ=11)
                                                                                                                 atatGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAAt                                      >  3:337561/1‑151 (MQ=255)
                                                                                                                  tatGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTAATCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGCAATg                                     >  2:1043845/1‑151 (MQ=255)
                                                                                                                     ggCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                                    >  5:86892/1‑149 (MQ=255)
                                                                                                                     ggCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                                    >  7:867649/1‑149 (MQ=255)
                                                                                                                       cTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTACTACGAAATATTCCTCAATGaac                                  >  5:927649/1‑150 (MQ=14)
                                                                                                                       cTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                                >  5:1046277/1‑151 (MQ=255)
                                                                                                                        ttttataggtttata‑‑AAT‑‑ATCTGCAGTAAACTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                                <  2:1060303/139‑1 (MQ=11)
                                                                                                                        tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAGATCtgccggaaggatgg                                 >  8:666502/1‑138 (MQ=11)
                                                                                                                         ttagtgtggaaaTC‑‑AAT‑‑ATCTCGAGTAATCTAAAATTCGCTATCCATCCGTAGCTCAGCTTGATAGAGTACTCGGCTACGAACCGCGGGGTCTGAGCTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTa                               <  7:229948/139‑1 (MQ=14)
                                                                                                                          tagtagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGa                                                                                      >  7:876316/1‑94 (MQ=255)
                                                                                                                          tagtagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                              >  6:1367965/1‑150 (MQ=255)
                                                                                                                          tagcatcGGAATG‑‑AAT‑‑ATCTGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                              <  1:463630/144‑1 (MQ=14)
                                                                                                                           agtagCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGAGATATTCAGTAATGACGGGAt                              >  8:1142414/1‑151 (MQ=11)
                                                                                                                            ataggtatata‑‑AAA‑‑ATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCg                            <  4:649570/143‑1 (MQ=11)
                                                                                                                             tagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCACGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                           >  4:1338974/1‑150 (MQ=255)
                                                                                                                              agCGTTATA‑‑AAT‑‑ATCTGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                          <  5:625604/150‑1 (MQ=255)
                                                                                                                              agCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCGAAATATTCAGCTATGAAGGTAtgc                            >  6:735246/1‑148 (MQ=11)
                                                                                                                              agCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCGATGCATGCGTAGCTCAGCTGAATAGAGTACTGTGGGACGAACCGATCTGTCCGAGGTTCGAATACTGCCGGATGCAAGATATTTTAATAAATATTCGGCAGTGAGGGtgtgga                           >  4:214924/1‑144 (MQ=9)
                                                                                                                               gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTTTCCGAAATCTTCATAAATGATCGTAtaca                           >  1:147970/1‑147 (MQ=11)
                                                                                                                                  ttata‑‑AAT‑‑ATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAgg                      <  6:695207/149‑1 (MQ=255)
                                                                                                                                   tgtatcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATATTCAGCAATGAAGGTATCGaat                         >  2:796750/12‑149 (MQ=11)
                                                                                                                                   tatcaaaaT‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGaa                                   <  3:618166/132‑1 (MQ=14)
                                                                                                                                   tATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAGCGAGCGGTCGGAGGTTCGAATCCTCCCGGAAGCACCATATTCTCCGACATCTTCAGCAATGAAGGTATCGATGAGGtg                    >  3:647542/1‑150 (MQ=255)
                                                                                                                                   tATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATCCATCCGTAGCTCAGCTGGATAGAGTACTCGCCTACGAACCGACCGCTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagagct                     >  7:5877/1‑148 (MQ=255)
                                                                                                                                    gatgttaaaaatCTGCAGTAAAGTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagag                       <  5:1336231/141‑1 (MQ=11)
                                                                                                                                    aTC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAg                   <  7:59994/151‑1 (MQ=255)
                                                                                                                                      atcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTGTCCGAGATCTTGAGGTATGAGGGGTTGGAagatct                     >  2:386578/9‑148 (MQ=14)
                                                                                                                                        caaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGACATATTCAGCAATGAAGGTATCGAAGAGGTac                   >  3:149458/7‑150 (MQ=14)
                                                                                                                                        ataaaaatCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTa                    <  3:952566/144‑1 (MQ=255)
                                                                                                                                        aaaT‑‑ATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg                 <  2:904840/150‑1 (MQ=255)
                                                                                                                                         aaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAGATCTTCAGGAATGAAGGGATGGAagagagagc                  >  6:1335760/6‑146 (MQ=11)
                                                                                                                                         aaaaaatCAGCAGTAAACTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGc                  <  3:553803/146‑1 (MQ=255)
                                                                                                                                         aaT‑‑ATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                <  6:107806/151‑1 (MQ=255)
                                                                                                                                         aaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGATGAGGTAGCgg                >  4:215840/1‑151 (MQ=255)
                                                                                                                                         aaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                >  4:28133/1‑151 (MQ=255)
                                                                                                                                         aaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                >  3:119300/1‑151 (MQ=255)
                                                                                                                                          at‑‑atCAGCAGTAAAATAAATTTCCAGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGa               <  1:892046/151‑1 (MQ=255)
                                                                                                                                           t‑‑atCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGa               <  2:290964/150‑1 (MQ=255)
                                                                                                                                           t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                             <  5:1003891/88‑1 (MQ=255)
                                                                                                                                           t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                             >  6:1002914/1‑88 (MQ=255)
                                                                                                                                           t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCACCAATGAAGGTATCGAAGAGGTAGCGGa               >  7:33271/1‑150 (MQ=255)
                                                                                                                                           aaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACGAAATATTCAGAAATGAACGTATAGataataagaacg                >  2:463657/4‑140 (MQ=1)
                                                                                                                                            aaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAGATCTTCAGCAATGAAGGTATGGAagagctcgagg                >  5:1166363/3‑143 (MQ=11)
                                                                                                                                              atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAGCTTCAGCAATGAAGGTATCGAAGAGATAGCGGTAt             >  5:616894/1‑151 (MQ=255)
                                                                                                                                                 tgcagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTAAATATTCAGAAATGAAGGTAAAGAATAGCTAgagtaaaaa           >  2:357302/2‑142 (MQ=11)
                                                                                                                                                 agcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTa           >  3:764183/1‑150 (MQ=255)
                                                                                                                                                  gcagTAAATTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt            <  1:876989/148‑1 (MQ=255)
                                                                                                                                                   cagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAGATATTGAGCAATGAAGGTTTCGAAGAGGACGAGGAATTTAc         >  3:409808/1‑150 (MQ=14)
                                                                                                                                                   cagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAAc         <  4:410118/150‑1 (MQ=255)
                                                                                                                                                   cagTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAAcc        <  1:633573/151‑1 (MQ=255)
                                                                                                                                                    agtaaagtaaGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATCTTCAGAAATGAAGGTATAGAATATCTAGAGGAATTa           >  4:418743/1‑147 (MQ=14)
                                                                                                                                                    agtaaagtaaATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCg       <  1:357279/151‑1 (MQ=255)
                                                                                                                                                    agTAAACTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTa           <  3:418461/147‑1 (MQ=255)
                                                                                                                                                     gTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTc                                                                                                            <  8:811955/49‑1 (MQ=255)
                                                                                                                                                      tAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCgta                                                  >  3:791861/1‑104 (MQ=37)
                                                                                                                                                      tAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCgta                                                  <  4:791630/106‑3 (MQ=37)
                                                                                                                                                       aaagTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTGAGCAATGAAGGTATGGAAGAGGTAGAGGAATTAACCgaac    >  1:414184/1‑148 (MQ=14)
                                                                                                                                                       aaaaTAAATTTCCCGATGCATCCGTATCTCAGCTCGATAGAGTACTCGGCTACGAACCGAGCGGTCGTAGGATCTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAg    <  2:70180/151‑1 (MQ=255)
                                                                                                                                                       aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc      >  2:295743/1‑149 (MQ=255)
                                                                                                                                                       aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc      >  8:1138043/1‑149 (MQ=255)
                                                                                                                                                       aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc      >  4:1120531/1‑149 (MQ=255)
                                                                                                                                                        aaataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAgg   >  6:305510/1‑151 (MQ=255)
                                                                                                                                                        aaataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAgg   >  3:94209/1‑151 (MQ=255)
                                                                                                                                                        aaataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAgg   >  2:1370468/1‑151 (MQ=255)
                                                                                                                                                         aataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGc  >  2:157680/1‑151 (MQ=255)
                                                                                                                                                          ataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCg                                                                                        <  2:1370191/64‑1 (MQ=35)
                                                                                                                                                          ataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCg                                                                                        >  1:1369836/1‑64 (MQ=35)
                                                                                                                                                          |                                                                                                                                                     
CTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGC  >  DH1/1065465‑1065763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑