Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6180AG30.6% 168.7 / 93.2 129intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (55/22);  new base G (27/7);  total (83/46)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 147 total aligned reads displayed.

AAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACT  >  DH1/1065473‑1065766
                                                                                                                                                      |                                                                                                                                                    
cgcttctcttccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTcc                                                                                                                                                 <  6:1348088/136‑1 (MQ=255)
    atacctccctttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGAt                                                                                                                                             <  7:470083/140‑1 (MQ=255)
      accgccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTTAATATCACATAAGAAGCTATATA‑‑AAA‑‑ATAAGCAGTAAAAAAAAATTccacaa                                                                                                                                             >  8:469886/10‑145 (MQ=2)
        cgccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAATACTTAATATGGCATTAGTAGCGATATA‑‑AAT‑‑ATAAGCAGTaaaaaaaa                                                                                                                                                      >  2:873890/8‑138 (MQ=14)
            cGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAATACGAGATATGCATGAAGTATCGTAAGC‑‑AAG‑‑ATCTCCAGTAAATAAAATAACCCGATACagacg                                                                                                                                      >  8:283118/1‑146 (MQ=2)
                gCATCGGTAGCACAGCTGGATTGACTACTCGGCTACTATCGCGTG‑GGTCGGCGCTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAg                                                                                                                                   <  7:648546/149‑1 (MQ=17)
                 cATCCGTAGCTCAGCTGGAGAGAGTACTCGGCTACGACC‑GGAGCGGTCGGAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTc                                                                                                                                <  7:352338/151‑1 (MQ=255)
                      gTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTAGATATTCCTAAATTAACGGAAAC‑‑AAT‑‑ATCATCAGTAATATAAATATCGCTATACATACGAAgaagatcat                                                                                                                           >  4:1360726/1‑143 (MQ=9)
                           tCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACTAGATATGGCGGTATGAGCGGTTGG‑‑AAG‑‑ATCTTGAGTAATTTAAATTTCGCGCTTCGTACGTATCTCATCTGAAt                                                                                                                        >  7:1062886/1‑149 (MQ=2)
                                tGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTTATATGGCTTTATTAGCGGTAGC‑‑AAT‑‑ATCACCAGTAAAATAAATTTCCCGATGCATACGTAGCTCAGcgttatacagaac                                                                                                                 >  5:160650/1‑139 (MQ=21)
                                tGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGAGATATTGAGGTATTATCGTTTAC‑‑AAT‑‑ATCTAGAGTAATATAAATGTCCCGATACATAAGTAACTAAGCTGGATAGAGTAc                                                                                                                 >  6:6907/1‑151 (MQ=9)
                                ggctttttctACTCGGCGACGATC‑GCGCCGCTCGGAGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTAc                                                                                                                 <  5:411668/142‑1 (MQ=14)
                                                       c‑cGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTtcc                                                                                                                                                >  1:833238/1‑97 (MQ=255)
                                                             cGGTCGGCGCGTCGAATCCGCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGGCGGAGGtt                                                                                     <  6:756291/151‑1 (MQ=255)
                                                              ggTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTgccc                                                                                                                                               >  1:591989/1‑91 (MQ=255)
                                                                    ggtgtctaaTCCTCCGTTATTCAACATCTCTTACTATATATGGCTTTACTAGCTTTATA‑‑AAT‑‑ATCAGCAGTAAACTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcc                                                                              <  4:136572/144‑1 (MQ=14)
                                                                    aGGTTCGAATCCTCCCGGATGCACCATATGTTACTTGATATTGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAATTAAATTTCCCGATGCCTCGGTAGCTCATCTGGATAGAGTACTCGGCGACGACCCGACCGGTCCGACGGTAGAAtcc                                                                              >  1:979326/1‑151 (MQ=14)
                                                                        tCGAATCCTCCCGGATGCACCATATCTTACTTGATATGCCGTAAGTAGGGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAAGTTCCCGATGCATCAGTAGATCAGGGGGGTAGCGTCCGCGGCTCCGCCCCGCGCGGTCGGAGGttggcagccgccc                                                                          >  3:525009/1‑140 (MQ=255)
                                                                           aagcctccCGGATTCAACATCTCTTACTTTATTTGGCTTTATTAGCTTTATA‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCg                                                                         <  6:930159/146‑1 (MQ=14)
                                                                           aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCggg                                                                       >  8:842288/1‑150 (MQ=39)
                                                                           aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAGC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCACGATGCATCCGTAGCTCAGGTGGATAGAGTACTCGGCTACGAACCGCGCGGTCGGAGGTTCGAATCCTCCCgg                                                                        >  1:70408/1‑150 (MQ=255)
                                                                             tcctccCGGATGCACCATCTATTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGATCAGCTGGATAGAGTACTCGGATACGAACCGAGCGGTCGGAGGTTCGAATACTCCCGGATg                                                                     >  2:892371/1‑151 (MQ=255)
                                                                              cgagctctctacaacATCTCTTAGTTGATTTCTCTTTAGTAGGTCTATC‑‑AAT‑‑ATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGc                                                                    <  1:292179/137‑1 (MQ=11)
                                                                                   cGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat                                                               >  4:1097467/1‑151 (MQ=39)
                                                                                   cGGATGCACCATCTCTTAATTGATATGGGTTTATTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCAGATGCATCCGTAGCTCAGATGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCat                                                               >  2:592164/1‑151 (MQ=255)
                                                                                      aTGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATAtt                                                            >  3:514837/1‑151 (MQ=39)
                                                                                        gCACCATCTCTTACTTGATATGGCTTTAGTAGAGGTATC‑‑AAT‑‑ATGAGCAGTAAATTAAATTTAACGATGCATACTTAGCTCAGCTGGATAGAGTAATCGGCTACGAGCCGAGCGGTCGGAGGTTCGAATCCTCCCGGCTggcgcagattcg                                                          >  3:876601/1‑140 (MQ=14)
                                                                                               ctctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAt                                                   >  8:59899/1‑151 (MQ=255)
                                                                                                  ttACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGTAAACtt                                                >  1:717798/1‑151 (MQ=255)
                                                                                                       tGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGc                                            >  6:543420/1‑150 (MQ=255)
                                                                                                        gATATGGCTTTACTAGCTGTATA‑‑AAT‑‑ATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCa                                           <  7:988770/150‑1 (MQ=11)
                                                                                                         atacGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATGCAga                                            >  2:196879/1‑149 (MQ=14)
                                                                                                          tatGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTAATCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGCAATg                                        >  2:1043845/1‑151 (MQ=255)
                                                                                                             ggCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                                       >  5:86892/1‑149 (MQ=255)
                                                                                                             ggCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                                       >  7:867649/1‑149 (MQ=255)
                                                                                                               cTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTACTACGAAATATTCCTCAATGaac                                     >  5:927649/1‑150 (MQ=14)
                                                                                                               cTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                                   >  5:1046277/1‑151 (MQ=255)
                                                                                                               cTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGATATATCAAAACATTACCGATGCATACGTATCTCTGCTTGAAATAGACATAGCCTTCTAACCGAGGTATAATAAGTTCGAATCGGCCCGGTTGCCCCATATTCTGGGAATTCTTGCGCAATGAAGGt                                   >  3:37486/1‑151 (MQ=255)
                                                                                                                ttttataggtttata‑‑AAT‑‑ATCTGCAGTAAACTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                                   <  2:1060303/139‑1 (MQ=11)
                                                                                                                tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAGATCtgccggaaggatgg                                    >  8:666502/1‑138 (MQ=11)
                                                                                                                tttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGAAATCTTCAGCAATGAAgg                                    >  4:1178474/1‑151 (MQ=11)
                                                                                                                tttAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                                   >  8:868417/1‑150 (MQ=255)
                                                                                                                 ttAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                                 >  8:705530/1‑151 (MQ=255)
                                                                                                                  tagtagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGa                                                                                         >  7:876316/1‑94 (MQ=255)
                                                                                                                   agtagATGTATC‑‑TAT‑‑TTCAGTAGTATAATATCTTTCGATCTGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCg                               <  8:1294413/151‑1 (MQ=11)
                                                                                                                    ataggtatata‑‑AAA‑‑ATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCg                               <  4:649570/143‑1 (MQ=11)
                                                                                                                      aggtttata‑‑AAT‑‑ATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                             <  4:174029/145‑1 (MQ=11)
                                                                                                                      agCGTTATA‑‑AAT‑‑ATCTGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                             <  5:625604/150‑1 (MQ=255)
                                                                                                                      agCGGTCTA‑‑AAT‑‑ATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                            <  7:508710/151‑1 (MQ=255)
                                                                                                                      agCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCGAAATATTCAGCTATGAAGGTAtgc                               >  6:735246/1‑148 (MQ=11)
                                                                                                                      agCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTACCGATGCATCCGTAACTCAGCTGAATAGCGTCCTGGGGGACGAACCGAGCGGTCCGAGGTTCGAATACTGGCGGATCCCACATATTAAAAGAAATCAGCGGCTATGAAGGGGTCGa                              >  4:221661/1‑149 (MQ=2)
                                                                                                                       gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTTTCCGAAATCTTCATAAATGATCGTAtaca                              >  1:147970/1‑147 (MQ=11)
                                                                                                                       gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGATATCTTCATGAATGAAGGtttgga                              >  5:1256227/1‑145 (MQ=11)
                                                                                                                       gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGCTCCGAGATATGCAgagaacagcgctcaaa                              >  1:422479/1‑135 (MQ=14)
                                                                                                                          ttata‑‑AAT‑‑ATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAgg                         <  6:695207/149‑1 (MQ=255)
                                                                                                                           tatcaaaaT‑‑ATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGaa                                      <  3:618166/132‑1 (MQ=14)
                                                                                                                           tATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAGCGAGCGGTCGGAGGTTCGAATCCTCCCGGAAGCACCATATTCTCCGACATCTTCAGCAATGAAGGTATCGATGAGGtg                       >  3:647542/1‑150 (MQ=255)
                                                                                                                           tATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATCCATCCGTAGCTCAGCTGGATAGAGTACTCGCCTACGAACCGACCGCTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagagct                        >  7:5877/1‑148 (MQ=255)
                                                                                                                              c‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg                    >  3:96788/1‑151 (MQ=255)
                                                                                                                              atcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTGTCCGAGATCTTGAGGTATGAGGGGTTGGAagatct                        >  2:386578/9‑148 (MQ=14)
                                                                                                                                caaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGACATATTCAGCAATGAAGGTATCGAAGAGGTac                      >  3:149458/7‑150 (MQ=14)
                                                                                                                                 aaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAGATCTTCAGGAATGAAGGGATGGAagagagagc                     >  6:1335760/6‑146 (MQ=11)
                                                                                                                                 aaT‑‑ATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                   <  6:107806/151‑1 (MQ=255)
                                                                                                                                 aaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGATGAGGTAGCgg                   >  4:215840/1‑151 (MQ=255)
                                                                                                                                 aaT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                   >  3:119300/1‑151 (MQ=255)
                                                                                                                                   t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                                <  5:1003891/88‑1 (MQ=255)
                                                                                                                                   t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                                >  6:1002914/1‑88 (MQ=255)
                                                                                                                                   t‑‑atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCACCAATGAAGGTATCGAAGAGGTAGCGGa                  >  7:33271/1‑150 (MQ=255)
                                                                                                                                   aaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACGAAATATTCAGAAATGAACGTATAGataataagaacg                   >  2:463657/4‑140 (MQ=1)
                                                                                                                                    attgccccctataaatagatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTATCGAAGAGATAGCggt                  <  1:375954/139‑2 (MQ=11)
                                                                                                                                     aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATCTTCAGCAATGAAGGTATCGAAGATGTAGCGGa                  >  1:291092/2‑150 (MQ=14)
                                                                                                                                      atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAGCTTCAGCAATGAAGGTATCGAAGAGATAGCGGTAt                >  5:616894/1‑151 (MQ=255)
                                                                                                                                         tgcagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTAAATATTCAGAAATGAAGGTAAAGAATAGCTAgagtaaaaa              >  2:357302/2‑142 (MQ=11)
                                                                                                                                         agcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTa              >  3:764183/1‑150 (MQ=255)
                                                                                                                                          gcagTAAATTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt               <  1:876989/148‑1 (MQ=255)
                                                                                                                                           cagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAGATATTGAGCAATGAAGGTTTCGAAGAGGACGAGGAATTTAc            >  3:409808/1‑150 (MQ=14)
                                                                                                                                           cagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAAc            <  4:410118/150‑1 (MQ=255)
                                                                                                                                           cagTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAAcc           <  1:633573/151‑1 (MQ=255)
                                                                                                                                            agtaaagtaaGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATCTTCAGAAATGAAGGTATAGAATATCTAGAGGAATTa              >  4:418743/1‑147 (MQ=14)
                                                                                                                                            agtaaagtaaATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCg          <  1:357279/151‑1 (MQ=255)
                                                                                                                                            agTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCg          >  3:1066146/1‑151 (MQ=255)
                                                                                                                                             gTAAAGTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCg                                                       <  3:162081/105‑1 (MQ=16)
                                                                                                                                              tAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCgta                                                     >  3:791861/1‑104 (MQ=37)
                                                                                                                                              tAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCgta                                                     <  4:791630/106‑3 (MQ=37)
                                                                                                                                               aaagTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTGAGCAATGAAGGTATGGAAGAGGTAGAGGAATTAACCgaac       >  1:414184/1‑148 (MQ=14)
                                                                                                                                               aaaaTAAATTTCCCGATGCATCCGTATCTCAGCTCGATAGAGTACTCGGCTACGAACCGAGCGGTCGTAGGATCTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAg       <  2:70180/151‑1 (MQ=255)
                                                                                                                                               aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc         >  8:1138043/1‑149 (MQ=255)
                                                                                                                                               aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc         >  2:295743/1‑149 (MQ=255)
                                                                                                                                                aaataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAgg      >  3:94209/1‑151 (MQ=255)
                                                                                                                                                aaataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAgg      >  6:305510/1‑151 (MQ=255)
                                                                                                                                                 attaagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGc     <  6:1165680/149‑1 (MQ=255)
                                                                                                                                                 atgtaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGc     <  3:263821/147‑1 (MQ=255)
                                                                                                                                                 agtaagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGCTCCGAGATCTGCAGGAATGAGGGGATAGAAGAGGTAGCGGAATTAACCTGAGGc     >  2:13537/3‑151 (MQ=14)
                                                                                                                                                 aataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGc     >  2:157680/1‑151 (MQ=255)
                                                                                                                                                  ataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCg                                                                                           <  2:1370191/64‑1 (MQ=35)
                                                                                                                                                  ataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCg                                                                                           >  1:1369836/1‑64 (MQ=35)
                                                                                                                                                   taagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGATCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagag                          <  1:1309734/128‑1 (MQ=25)
                                                                                                                                                    aaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCg                                                                                           <  5:1256050/62‑1 (MQ=34)
                                                                                                                                                      atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTa                                                                                                          >  7:648267/1‑45 (MQ=31)
                                                                                                                                                      atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTGTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACt  >  4:1134712/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
AAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACT  >  DH1/1065473‑1065766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑