BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	2,326,287	0	G	T	27.4%	640.7 / 79.6	353	intergenic (+207/+200)	yddM/fdnI	transcriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
Reads supporting (aligned to +/- strand): ref base G (163/86); new base T (17/77); total (182/171)
Fisher's exact test for biased strand distribution p-value = 1.41e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATA  >  DH1/2326137‑2326435

gATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTg                                                                                                                                                      <  5:28987/151‑1 (MQ=32)

 aTGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTg                                                                                                                                                      >  5:501889/1‑150 (MQ=32)

 aTGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGa                                                                                                                                                     <  7:60621/151‑1 (MQ=33)

  tGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTAAGATGACTACATTATGAAAATGAATCATTTTAGAGCGCCATTTGa                                                                                                                                                     >  4:95483/1‑150 (MQ=11)

   gcgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGaa                                                                                                                                                    <  1:1309590/150‑1 (MQ=33)

   gcgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGaa                                                                                                                                                    <  1:852425/150‑1 (MQ=33)

    cgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTAAAATATGAAAATCACGCTTTTTACATCGCCAATAGaaa                                                                                                                                                   >  5:659386/1‑150 (MQ=11)

     gcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACt                                                                                                                                                 <  6:296759/151‑1 (MQ=35)

     gcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAACAACATATGAGACt                                                                                                                                                 >  8:1143023/1‑151 (MQ=255)

       tACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtg                                                                                                                                                >  1:665885/1‑150 (MQ=37)

        aCGCTTATCAGGCCTACACGTTGACTGCAATCTATTGGATTTGTAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtgat                                                                                                                                              <  3:635198/151‑1 (MQ=255)

        aCGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtg                                                                                                                                                <  3:599907/149‑1 (MQ=37)

           cTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTAGAAACTAATAAc                                                                                                                                           >  4:744210/1‑151 (MQ=19)

           cTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATCAAGCATTTTACAGCGCCATTTGAAACtgatga                                                                                                                                            >  6:779281/1‑150 (MQ=255)

           cTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCCGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtgatta                                                                                                                                            >  7:932104/1‑148 (MQ=255)

             tATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGAc                                                                                                                                           <  5:318229/149‑1 (MQ=40)

              aTCAGGCCTACACTTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACaaa                                                                                                                                        <  8:755230/151‑1 (MQ=255)

              aTCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACaaa                                                                                                                                        <  2:1365197/151‑1 (MQ=255)

               tCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACtgatgcaaaa                                                                                                                                        >  2:786195/1‑145 (MQ=255)

                       acacGTTCACTGCAATCTATTGGATTTTAAGGGTTTTCATGCCGTATAAGTCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACAtt                                                                                                                               <  2:889588/151‑1 (MQ=255)

                       acacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGCAANTGATGACAAACGAAACAtt                                                                                                                               >  3:1332589/1‑151 (MQ=255)

                        cacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTCTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATAACAAACGCTACAt                                                                                                                                >  6:174454/1‑149 (MQ=255)

                            ttCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGGCAGAGGCAACATTGCGTg                                                                                                                          >  4:832179/1‑151 (MQ=255)

                            ttCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGAAACATTTCCTg                                                                                                                          >  1:624602/1‑151 (MQ=255)

                            ttCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGACATATGAAACTCATCAAAAACGCaaaaattcaa                                                                                                                           >  2:58399/1‑142 (MQ=14)

                             gcACTGCAATCTATTGGATTTGACGCGGTTGCATGCCGGATAATTCTTTCACTCAGCATGCGTCATGAACTAATACTACGTTAGCTTCAATATGAACATGCCGCCTTCTACATCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGa                                                                                                                         <  7:1300111/150‑1 (MQ=12)

                                 ggCAATCTATTGGATTTGAATGTTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGTCATTAACAAAGCCTACGTTGTCTACAATATGAAAATGACCCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATgcg                                                                                                                     <  1:813797/150‑1 (MQ=17)

                                  gCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACCAAACATTGACtcaagagc                                                                                                                    >  3:1363080/1‑144 (MQ=255)

                                  gCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGACATATGAAAATCATCAGAAACGCaaaaattcattaagcc                                                                                                                     >  4:51861/1‑136 (MQ=11)

                                   cAATCTATTGGAGTTGAAGGGTTTTCTTGCCGGCTCTGTCGTTCAAGCCGCTTCCTGAATGAACAAAGCCTTCGTTGTCTTCAATATGAAAATGTCTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATgcgc                                                                                                                    <  2:394889/150‑1 (MQ=25)

                                    aaTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATAACAAACAAAACAttaactcattagct                                                                                                                   >  6:492105/1‑138 (MQ=14)

                                    aaTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCt                                                                                                                   >  2:1300502/1‑150 (MQ=255)

                                    aaTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTCATGACAAACGCAACATTGCCTGATGCGCt                                                                                                                   >  7:997985/1‑150 (MQ=255)

                                     aTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTAc                                                                                                                 >  5:84963/1‑151 (MQ=255)

                                     aTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCATGATGCGATAc                                                                                                                 >  4:1117736/1‑151 (MQ=255)

                                         aTTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCtt                                                                                                             >  1:70964/1‑151 (MQ=255)

                                              aTTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCCCTACGCTTATc                                                                                                          >  6:1206815/1‑149 (MQ=255)

                                               tttGAAGGGTTGTCAAGCCGTTTCAGCCGTTAACGCCGGATCCTGTATGAACTAAGCCTACGTTTTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAg                                                                                                        <  6:1286170/150‑1 (MQ=25)

                                                  gAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                                    >  6:458836/1‑151 (MQ=255)

                                                       gTTTTCATGCCGGATAAGGCGTTCACGACTCATCATGAATGAATTAAGCATACGTTGTCTATAATTTGATAATGACTCCTTTTAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTacac                                                                                                <  8:381650/150‑1 (MQ=18)

                                                         tttCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGACTGATGCGCTACGCTTATCAGTACTACACGtt                                                                                             >  2:1167449/1‑151 (MQ=255)

                                                          ttCATGCCGTATAAGCCGTTCACGACGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGtt                                                                                             <  6:917190/150‑1 (MQ=255)

                                                           tCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAACCTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTcc                                                                                           >  7:1009180/1‑150 (MQ=255)

                                                            cATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCAc                                                                                          >  7:259447/1‑151 (MQ=255)

                                                              tGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTg                                                                                        >  3:1241798/1‑151 (MQ=255)

                                                                gcGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCa                                                                                      <  5:337350/150‑1 (MQ=255)

                                                                  ggATAAGTCGTTCAAGCCGCATCCGGAATGAACAAAGACTACGTTTTCTACAATATCAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAAt                                                                                    <  1:953409/151‑1 (MQ=21)

                                                                  ggATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGAAACATTGCCTGATGATATACGCTTATCAGGCCTACACGTTCACTGCAAt                                                                                    >  5:998532/1‑151 (MQ=255)

                                                                   gataccgCGGTCACGCCGCATCCTGCATGAACAAAGCCTACGTGTTATACAATATCAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATc                                                                                   <  5:12275/145‑1 (MQ=21)

                                                                     tAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGCCGCCTTTTACAGCGCCATTTGAAACTGATGAAAAAAGAAACATTGACTGATGAGATACGCTTATCATGCCTACACGTTCACTGCAATCt                                                                                  >  4:1168373/1‑150 (MQ=21)

                                                                     tAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACCCGTTCACTGCAATCtt                                                                                 >  7:376015/1‑150 (MQ=255)

                                                                      aaGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTAt                                                                                >  1:284821/1‑151 (MQ=255)

                                                                          cGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                             >  2:46267/1‑150 (MQ=255)

                                                                          cGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                             >  6:1132961/1‑150 (MQ=255)

                                                                           gTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTAAAATATGAAAATGACGCCTGTTACAGCGCCATTAGAAAATGAAGACAAACGCAACATTGCATGATGCGATACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAt                                                                           >  3:1268582/1‑151 (MQ=25)

                                                                            ttCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAt                                                                           <  8:179188/150‑1 (MQ=255)

                                                                              caagagtcATCCTGCATGTAATAAGCCTTCTTTTTATACAATATTAAAATGACTCTTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAttt                                                                         <  2:623261/143‑1 (MQ=14)

                                                                              cACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTg                                                                        >  5:879446/1‑151 (MQ=255)

                                                                              aaCGCCGCATACTGGATTAACATAGCCTACGTTCTTTAGATTTTCAAAATGCCCCCTTTTAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTg                                                                        <  4:496940/150‑1 (MQ=14)

                                                                               acttataataaggCATGAACAAAGCCTACTATCTATACAATATGAAAATTACGCTTCTTCCAGCTCTATTTTTATCTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTg                                                                        <  6:1055579/139‑1 (MQ=17)

                                                                               aCGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTg                                                                        >  5:1138721/1‑150 (MQ=255)

                                                                                cgccgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGa                                                                       >  8:1065461/1‑150 (MQ=255)

                                                                                    gcATCCGGCATGAACAAAGCCTACGTTTTATACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAGCATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGt                                                                  <  3:377730/151‑1 (MQ=255)

                                                                                      aTCCGTCATTAACAAAGCCTACGTTGTCTACATTATGAAAATGACCCTTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATT‑CCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGtttt                                                               <  4:393165/151‑1 (MQ=18)

                                                                                       tCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACTCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGtttt                                                               >  5:154002/1‑151 (MQ=255)

                                                                                              ttaaCAATGACTACTTTTTATTCAATATAAAAATGCCTCTTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGca                                                         <  1:740405/148‑2 (MQ=11)

                                                                                                aaCAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCgg                                                       >  1:207972/1‑150 (MQ=255)

                                                                                                  cagaGCCTTCGTGGTCTAAAATATTAAAATGCCGCCTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATa                                                    <  3:960695/148‑1 (MQ=14)

                                                                                                  cAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGTAt                                                     >  5:80013/1‑150 (MQ=255)

                                                                                                    aaGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATa                                                    >  7:460020/1‑149 (MQ=255)

                                                                                                    aaGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGCTTTTCATGCCGGATa                                                    >  8:57262/1‑149 (MQ=255)

                                                                                                     aGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCAGGATAAgg                                                 >  3:338002/1‑151 (MQ=255)

                                                                                                     aGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAgg                                                 >  7:1377444/1‑151 (MQ=255)

                                                                                                      gCCGACGTTGTCACCAATATGAAAATGCCGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGc                                                <  1:324996/151‑1 (MQ=255)

                                                                                                        cTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGcct                                              >  3:498974/1‑149 (MQ=255)

                                                                                                             ttGCCTACAATATGATAATGAGTTCTTATAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCa                                           <  2:832869/149‑1 (MQ=11)

                                                                                                              tGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgc                                        >  7:1249636/1‑151 (MQ=255)

                                                                                                              tGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGCCGTTCAcgc                                        >  1:16207/1‑151 (MQ=255)

                                                                                                                tCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccg                                      >  1:103032/1‑151 (MQ=255)

                                                                                                                  tACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccgc                                     >  1:39973/1‑150 (MQ=255)

                                                                                                                  aagactatGCATATGACGCCTCTTCCCCCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCa                                    <  5:588284/146‑1 (MQ=11)

                                                                                                                   aCAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                   >  1:1289251/1‑151 (MQ=255)

                                                                                                                   aCAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                   >  7:86235/1‑151 (MQ=255)

                                                                                                                     aaTATGAACATGCCGCCTGTTACATCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATcc                                 <  1:5457/151‑1 (MQ=255)

                                                                                                                        atGAAAATGACTACTTTTACAGCGCCATTTGAAACTGCTGACAAACTCAAGATTGCGTGCTGCTCTACGCTTATCATGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGc                              <  3:1167190/151‑1 (MQ=14)

                                                                                                                          gAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                            <  2:1236487/151‑1 (MQ=255)

                                                                                                                           aaaaagaacacTTTTACATTCCGATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                            <  2:964901/140‑1 (MQ=11)

                                                                                                                           aaaaTGAAGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                           <  8:925465/151‑1 (MQ=255)

                                                                                                                            aaaTGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                           >  7:1153853/1‑150 (MQ=255)

                                                                                                                             aattaagtcTTTTACAGAGCAATTTGAAACTTATTACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa                         <  8:6186/143‑1 (MQ=14)

                                                                                                                             aaTGTCGCCTTTTAGTGCGCCATTTTAATCTGATGACTAACGCAACATTGGGTGTTGCGCTAGGATTATCTGGCCTACAGGTTCTCTGAAATCTATTGGATTTGAAGGGTTTTCATGCGGGATAAGGCGTTGACGCCGCATCCGGCATGaa                         <  8:495956/151‑1 (MQ=11)

                                                                                                                             aaTGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa                          >  5:67254/1‑150 (MQ=255)

                                                                                                                                     gtattccAGCGCCCTTTGAAACTGATGACAACCGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                 <  1:1090785/145‑1 (MQ=255)

                                                                                                                                     cTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGTATAAGGCGTTCACGCCGCATCCGGCATGAAAAAAGCCt                 >  8:1304329/1‑151 (MQ=255)

                                                                                                                                       tttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTa                <  7:740385/150‑1 (MQ=38)

                                                                                                                                         tACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGt             >  2:699043/1‑151 (MQ=35)

                                                                                                                                              cgcCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCt        >  5:1115745/1‑151 (MQ=33)

                                                                                                                                               gcCATTTGAAACTGATGACAAACGCAACATTGTTTTTTTCTATAATCTTTTCAGGCCTACACGTTCACTGCTATCTATTGGTTTTGAAGTCTTTTCATGTCTTATTAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTa       <  4:1268619/151‑1 (MQ=11)

                                                                                                                                               cccATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTa       <  4:162561/150‑1 (MQ=18)

                                                                                                                                                ccATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTAc      >  3:849629/1‑151 (MQ=33)

GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATA  >  DH1/2326137‑2326435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`