Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,945,5350TA21.9% 379.6 / 38.3 232P787P (CCA→CCTftsKcell division protein FtsK
Reads supporting (aligned to +/- strand):  ref base T (53/122);  new base A (43/6);  total (98/134)
Fisher's exact test for biased strand distribution p-value = 2.79e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

CGTCGGTTTATGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGGTTGTTGCGGCTGCTGTACAGGT  >  DH1/2945385‑2945685
                                                                                                                                                      |                                                                                                                                                       
 gtcgGTTTATGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCtgtg                                                                                                                                                        <  2:1234987/151‑1 (MQ=255)
   cgGTTTATGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACGGGTTGTTGCGGCTGCTGATATTGATGCTGAGgc                                                                                                                                                      >  5:1205879/1‑151 (MQ=255)
   cgGTTTATGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTgg                                                                                                                                                       <  2:584067/150‑1 (MQ=255)
   cgGTTTATGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGgc                                                                                                                                                      >  3:1345968/1‑151 (MQ=255)
       ttATGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCa                                                                                                                                                   <  5:1076222/150‑1 (MQ=255)
        tATGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTCTGGAGCAAc                                                                                                                                                 >  5:47499/1‑151 (MQ=255)
          tGCAAGGGACGGGTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAAcc                                                                                                                                                <  6:1383169/150‑1 (MQ=255)
          tGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGAGCAACCGGTTGTTGCGGCTGCTGATATAGATGCTGAGGAGCAAc                                                                                                                                                 >  6:189171/1‑149 (MQ=255)
          tGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGAGCAACCGGTTGTTGCGGCTGCTGAAAGTGCTGCTGAGGCGCaggcg                                                                                                                                               >  7:216331/1‑147 (MQ=255)
           gCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTCTTGCGGCTGCTGATATTGATCCAGAGCCGCAAcccg                                                                                                                                              >  8:324764/1‑149 (MQ=37)
             aaCGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGgtt                                                                                                                                            <  3:1034076/151‑1 (MQ=255)
             aaCGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTAGTTGGGGCTGCTCATCTAGCGGCTGTGGCGCACCGGgtt                                                                                                                                            >  1:1052894/1‑151 (MQ=37)
                ggacggCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGgttgtt                                                                                                                                         <  7:291904/151‑1 (MQ=255)
                  acggCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGAGGCGCAACCGGTTGTTGc                                                                                                                                       >  5:1109572/1‑151 (MQ=255)
                    ggcgggcGCCATTACGCATCAACAGCGGAGGAAGCAGGGTAGCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCgg                                                                                                                                     <  8:552400/145‑1 (MQ=255)
                    ggCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCgg                                                                                                                                     <  1:1090067/151‑1 (MQ=255)
                     gCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGGGGAGAAAcgcggaggaccggg                                                                                                                                    >  3:468547/1‑138 (MQ=25)
                       tGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGAGGAGCAAGAGGTTCTTGCGgctg                                                                                                                                  >  1:291073/1‑151 (MQ=255)
                        gTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGgct                                                                                                                                   <  2:35357/149‑1 (MQ=255)
                        gTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGGGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGgct                                                                                                                                   <  2:989039/149‑1 (MQ=255)
                         tCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGAGCAACCGGTTGTTGCGgctgct                                                                                                                                >  7:1252798/1‑151 (MQ=255)
                           gCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGa                                                                                                                              <  8:1063136/151‑1 (MQ=255)
                             cATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGata                                                                                                                            <  8:1154519/151‑1 (MQ=255)
                             cATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGATGTGGAGCAACAGGTTGTTGCGGCTGCTGaa                                                                                                                             >  1:127854/1‑149 (MQ=255)
                              aTTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGGTGATGATGCGCCCGCGGTTGTGGCGGCtgatgagc                                                                                                                            >  3:971062/1‑148 (MQ=25)
                                 aCGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATAGTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATtgt                                                                                                                        <  2:645708/151‑1 (MQ=255)
                                  cGCATCAACAGGGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATtgtg                                                                                                                       <  3:543227/151‑1 (MQ=255)
                                  cGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATtgtg                                                                                                                       <  4:547222/151‑1 (MQ=255)
                                  cGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATtgtg                                                                                                                       <  5:788014/151‑1 (MQ=255)
                                     aTCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGAGGAGCAACCGGTTGTTGAGGCTGCTGATATtgttcat                                                                                                                    >  8:244543/1‑147 (MQ=255)
                                       cAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTg                                                                                                                  <  4:946738/150‑1 (MQ=255)
                                        aaCAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTgcg                                                                                                                <  1:93774/151‑1 (MQ=255)
                                            gCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTgcggcg                                                                                                             <  6:1205930/150‑1 (MQ=255)
                                              ggATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACAGGTTGTTGGGGCTGCTCATATTGCTGCTGAGGAGCCGCACACTGGTGCGCCTGCTGAGATtggtcaagcagcgctc                                                                                                           >  3:830694/1‑137 (MQ=18)
                                               gATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGATGCTGAGTAGCAACAGGTTCTTGATGCTGATGATAATGTTCCTgcagtgg                                                                                                            >  8:932513/1‑143 (MQ=18)
                                                 tGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGATGCTGTGGGGCAACCGGTTGTTCCGGCAGTATAGATAGTGGCTGGCGgcgcccgg                                                                                                        >  3:271165/1‑147 (MQ=18)
                                                   aaGCAGGGTAGCCTGCGGTCGTGGCCCAACTGGCTCTTGTGGCTGCTGATACTCCGGCTGCGGCGCAACCGGTGGTTGCGGCGGCTGATATTGCTGCTGTGGCGCAACCGGGTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGc                                                                                                     <  6:1145937/151‑1 (MQ=255)
                                                     gcggaggcacttccGGATGAGGCGCAAACGGTTGATGCGGCTGCGGATCGTGGGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGGTtg                                                                                                   <  1:748943/138‑1 (MQ=18)
                                                      cAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCtgt                                                                                                  <  4:621974/151‑1 (MQ=255)
                                                       aGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCtgtt                                                                                                 <  5:324414/151‑1 (MQ=255)
                                                       aGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACAGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCACCCGGCtgat                                                                                                 >  2:1150834/1‑149 (MQ=255)
                                                        gggTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCtgttg                                                                                                <  1:36550/151‑1 (MQ=255)
                                                            aTCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTgg                                                                                             <  8:741149/150‑1 (MQ=255)
                                                            aTCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCACAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTgg                                                                                             <  7:868645/150‑1 (MQ=255)
                                                              ccTGCGGTTGTGGGGCAAGTGGCGGTGGTTGCTGCTGATACTGCGGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTTATATTGTGGCTGCGGCGCAACCGGCTGTTGTGgct                                                                                           <  6:289011/150‑1 (MQ=21)
                                                              ccTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGgctg                                                                                          <  1:1012696/151‑1 (MQ=255)
                                                              ccTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGATGAGGAGCAACAGGATCTTGAGGCTGCTGATCATCTTCCTGCCGCGCAATCTGCtgttgt                                                                                               >  5:269101/1‑146 (MQ=17)
                                                              ccTGCGGTTGTGGCGCAACTGGCTGTTGGGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGgct                                                                                           <  4:788113/150‑1 (MQ=255)
                                                                 gCGGGTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGctgctg                                                                                       <  3:1087135/151‑1 (MQ=255)
                                                                   ggTTGTGGCGCAACTGGCTGTTGTCGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCATCCGGCTGTGGTGGCTGCTGAt                                                                                     >  6:1088675/1‑151 (MQ=255)
                                                                       ttggctgtaCTGGCTGTTGTGGCTGCTGATAGTGCTGCTGGGGCGCTACGGGTTGTTGCGTGTGCTGATATTGGTGCTGTGGCGGAACCTGTTGTTGCTGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATAct                                                                                  <  7:43688/142‑1 (MQ=255)
                                                                       ggggCGCACGTGGCTGGTGGGGCTGCTGATCCTGCTGCTGCGGCGCACGCGGTTGATGCTGCTGCGGAGATTGCGGCTGTGGCGGAACCGGGTGTTGCGGCTGCGGATAGTGGGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATActg                                                                                 <  7:1180117/149‑1 (MQ=255)
                                                                         ggCGGAAGTGGCTGTTGCGGCTGCTGATACTCCGGCTGCGGCGGAACCGGTTGATGCGGCTGCTGATTTTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATActgcg                                                                               <  1:508296/151‑1 (MQ=21)
                                                                            gcAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGAGGAGCAACAGGTTGTTGCGGCTGGTGAAAATGTTCCTGCCTCGCAATCGGCTGAGGTGCCTGCTGATaatcagtc                                                                             >  3:636510/1‑143 (MQ=17)
                                                                             cAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGATGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGTCGCAACCGGCTGTTGTGGCTGATGATActcaggctg                                                                           >  7:307870/1‑151 (MQ=255)
                                                                              atgtGGCTGTTGTTGCTTATGCTTCGTCTGCTGCGGCGTCTTCGGTTTTTGCTTCTGATGTTTTTGGTGATTTGGCTTAACCTGTTGTTGATGCTGGTTATATTGTGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATActgcggctg                                                                           <  2:46715/147‑1 (MQ=9)
                                                                                ggggCGGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGAGATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATActgcggctgcgg                                                                        <  7:323495/149‑1 (MQ=255)
                                                                                cTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGAGGCGCAAGACGTTGTTGCGCCTGCTGATATTGTGGCTGCGCCGCAATCTGCTGTTGTGCATGCTGATActgcggcagccc                                                                        >  7:611306/1‑149 (MQ=21)
                                                                                 tGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATAATGCTGCTGAGGAGCAACCGGTTGTTGCTGCTGCTGATAATGTTCCTGAGGCGCATACATCTGGTGTGCGTGATGATAatgctgctgcaac                                                                       >  3:1282408/1‑148 (MQ=14)
                                                                                  ggCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTTGCTGTCGAGCAAACGGTTCTTGCGCCTGCTGAAAATGTGCCTGCGCCGCAAACAGCTGAGGTGCCTCATGATActccggctccaccg                                                                      >  5:1332887/1‑145 (MQ=12)
                                                                                   gCTGTGGTGGCTGCTGCTCCGGCTGCTGCGGCGCCCCCGGTTGTTGCGGCGGCTGCTAGTGCGGCTGTGGCGCAACCGGGTGTTGCGGCTGCTGATACTGGGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGcggcgg                                                                     <  5:579516/151‑1 (MQ=37)
                                                                                       ttgtGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAAcg                                                                 >  8:289693/1‑150 (MQ=255)
                                                                                               gctgctcctgaggctgcGGCGTAAGCGGTTGTTGCTGCTGCTGATTTTCCGGCTGTGGCTGAACCGGTTGTTGCTGCTGCTGATATTGCGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGgttgt                                                           <  1:871507/137‑1 (MQ=25)
                                                                                               gatgATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCGGCTGTGGCGCAACCGGGTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGgttgtt                                                          <  3:865759/148‑1 (MQ=255)
                                                                                                  gatcatcaggctgcGGCGGATCCGGTTGTTGCTGCGGCGGAGTGTGGTGATTTGGCGCCACCGGGGGTTGAGGCTGGTGATATTGCGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCgg                                                      <  4:1304306/142‑1 (MQ=16)
                                                                                                  gATACTGCTGCTGCGGCGCAAGCGGTTTTTGCTGCGGCGGAAATTCCGGCTGAGGCGGAACCGGTTGTTGAGGCTGCTGATACTGCGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCg                                                       <  1:618324/150‑1 (MQ=17)
                                                                                                   atcctgctgctgCGGCGCAACCGGTTGATGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGATGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGgc                                                     <  3:1178291/148‑1 (MQ=255)
                                                                                                    tACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTCCGGCTGCTGAAAATGTGGCACCTGCGCAACCGCCTGTGCTGGCTGCTGCAACTGAGGCTGCTGCCGAACTCGTTGTTGCGgcg                                                    >  1:1168713/1‑150 (MQ=18)
                                                                                                     aCTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGAGGCGCACCCGGTTGGTGCGGCTGCTGAGCTTGTTGCTGCGGCGCAAGCGTCTGCGGTGGCTGCTGATCATCCGGCTCCCCCGGATGCGGTGGTGGCGgctg                                                   >  5:1018519/1‑151 (MQ=12)
                                                                                                        gcggctgcGGCGGATGCGGTTGTTGCTGCGTCTGATTTTGGGGCTGTGGCTCAACCGGTTGTTGCTGCTGGTGATATTGCGGTTGAGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGctgctg                                                <  6:23841/148‑1 (MQ=18)
                                                                                                           gctgcGGCGCAACCGGTTGTTGCTGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGata                                             <  8:536835/151‑1 (MQ=255)
                                                                                                           gctgcGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGat                                              <  7:67057/150‑1 (MQ=255)
                                                                                                             tgcGGCGCACCCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGGGCAACCGCCTGTTGGGGCTGCTGCTACTGCGCCTGCGGGGGAACTGGTTGTTGCGGCTGCTGATAtt                                           >  7:960510/1‑151 (MQ=21)
                                                                                                              gcggcgCATCCGGTTGTTGCGGCTGCTGATAGTCCGGATGAGGCGCAACCTGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATtg                                          <  8:436924/151‑1 (MQ=21)
                                                                                                               gggcgtatgcGGTTTTTGCTGCTGAGGATTTTGGGGCTGTGGCTAAACCGGTTGTTGATGCTGCTGATATTGGGGATGCGGCGCAACCGGCTGTTGTGGCTGCTGATAATGCTGCTGCGGCGTAACTGGTTGTTGCGGCTGCTGATATtgg                                         <  7:911409/142‑2 (MQ=12)
                                                                                                                 gcggaggcGGGTGTTGCTGCTGCGGATATTGCGGATGTGGCGCAACCGGGTGATGCGGCTGCTGATAGTGGGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATtgctg                                       <  3:168802/144‑1 (MQ=18)
                                                                                                                   gcAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGAGCAACCGGTTGTTGCGGCTGCTGATATTGTTCATGCCTCGCAATCCGCTGCTGTTGCTGCTGATCCTGCTGCTGCCCCGGATGGGGTTGAGCCGGGTGATGATATtcctgcg                                     >  1:493025/1‑150 (MQ=12)
                                                                                                                    gatgcGGTTGTTGCTGCTGATGAAGTTGCTGCTGTGGCGCCTTCTGTTGTTGCTGCTGCTGATATTGTGGCTGAGGCGCAATCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGCAACTGGTTGTTGCGGCTGCTGATATtgctgctg                                    <  4:75630/147‑1 (MQ=18)
                                                                                                                    cAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGAGCAACCGGTTGTTGCGGCTGCTGATAATGTTGCTGCGACGCAAGCATCTGATGTGGGTGATGATAATGATGCTGCGGCGGATTGGGTTGATCCGGGTGCTGATATtgctgcgg                                    >  5:947976/1‑149 (MQ=16)
                                                                                                                     cgcgggTTGATGCGTCTGCTGCCTTTGGGGCTGAGGCGGCACCGGTTGATGCGGCTGCGGATAGTGGGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATtgctgctg                                    <  3:752953/146‑1 (MQ=18)
                                                                                                                     aaCCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATtgctgctgc                                   <  5:620992/151‑1 (MQ=255)
                                                                                                                         ggTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGAGGCTGCTGATATTGTGGATGCTGAGCAAACGGCTGATGTTGCTGCTGATACTGCGCCTGCAGCGGAACTCGTTGTTGAGGATGATGATAATGATGCTGCGGTg                               >  4:1349228/1‑151 (MQ=21)
                                                                                                                          gaggtggccgctgatGATTTTCCTGCTGTGGCGGAAGCGGTTGATGCTGCTGCGGATATTGGGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGc                              <  8:606989/142‑1 (MQ=21)
                                                                                                                             gttgCTGCTGTTGATTTTGGTTCTTTGGGTTCACCGGTTGTTGATGCTGCGTTTTGTGGGGTTGCGGCGGAACCGGCTGTTGTGGCTGGTGATAGTGCGGGTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCaa                            <  4:327153/150‑1 (MQ=12)
                                                                                                                             gtggccgctgctGATCTTGCTGCTGTGGCGTCACGGGTTGTTGCTGCTGCTGATATTGGGGATGAGGCTCAACGGGCTGTTGTCGCTGCTGATACTGCGGCTGGGGCGCAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCaa                            <  4:1085840/144‑1 (MQ=18)
                                                                                                                               tgCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGCCAGCGGCGCAAAAGGCAATTGTTGCTGATGAAAATGATCCTGAGGAGGAATTTGTTGATGAGGGTGCTTATATTGCTGCTACAGTGGAAACg                         >  5:70254/1‑151 (MQ=12)
                                                                                                                                gcttctgctgAATATGCTGCTGTGGCGTATCGGGTTGTTGCTGCTGCTGATAATGGGGCTGTCGCGCAACGGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCgg                        <  1:445175/147‑1 (MQ=18)
                                                                                                                                   gctgctGTTTTTGCGGCTGTGGCGGAATCGGTTTTTGCTGCTGCTGATTTTGGGGTTTTGGCTGAACGGGCTGTTGTTGCTGCTGATAGTGCGGCTGCGGCGGAACTGGGTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGgttg                     <  6:1129410/151‑1 (MQ=18)
                                                                                                                                      gctgcattggaggctgCGGCGTCACCGGTTGATGCTGCTGCGGATCGTGGGGTTTGGGCGGAACGGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGGTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGgttgtt                   <  8:1305698/138‑1 (MQ=14)
                                                                                                                                      gatgATCTTCCTGCTGCGGCGGATTCGGTTGATGCTGCTGCTGTTATTGTCGATGCGGCTCAACCGGCTGTTGTTGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGgttgtt                   <  6:662212/148‑1 (MQ=18)
                                                                                                                                           tattcgtgctgTGGCGGAATCGGTTGTTGCTTCTGCTGATTGTGTGGCTGAGGCTCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGGTTGTTGCGgc              <  8:975154/144‑1 (MQ=18)
                                                                                                                                            tttGCCGCTGTGGCTGCACCTGTTGTTGCGGCTGCGGATAGTGGGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGGTTGTTGCGgct             <  7:1204054/149‑1 (MQ=18)
                                                                                                                                             ttGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGAGCACCGGGGTGTAGAGGCTGCTGGAACTGCGGAAGCGGCGGACCTGGTAGTTTCGGCTGCTGTGATTGCTGAAGCGGTGCTCCCGGTGGTTGCGgcggg           >  5:1132969/1‑148 (MQ=16)
                                                                                                                                               ggggctgTGGCGCGCCCGGTGGCTGCGGCGGCGGGCCGTGGGGCTGAGGCGGCCCCGGCGGTTGCGGCTGCGGATCCTGCGGGTGCGGCGCAACTGGGTGTTGCGGCTGCTGATAGTGCTGCTGCGGCGCAACCGGTTGTTGCGGctgctg         <  1:743866/148‑1 (MQ=2)
                                                                                                                                                  tctgTGGTGCCATCGGCTGTTGTGGCTGGTTATTGTGTGGATGCGGCGGAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGGGCAACCGGTTGTTGCGGCTGCTGTa       <  2:1087835/149‑1 (MQ=17)
                                                                                                                                                  gctgTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCATCCGGTTGTTGCGGCTGCTGTAc      >  6:437889/1‑151 (MQ=255)
                                                                                                                                                  gctgCGGCGGCACCGGGTGTTGCGGCTGCTGATCGTGGGGCTGCGGCGCAACCGGCTGATGTGGCTGCGGATACTGCGGCTGCGGCGGAACTGGGTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGGTTGTTGCGGCTGCTGTAc      <  7:940999/151‑1 (MQ=18)
                                                                                                                                                   ctgTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGAGCAACAGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAAGTGGTTGTTGAGGCTGCTGATATTGCTGCTGCGGTGCAACCGGTTGTTGCGGCTGCTGAAc      >  3:394688/1‑150 (MQ=255)
                                                                                                                                                    tgtgGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGGTAGTTGCGGCTGCTGTAc      >  8:191183/1‑149 (MQ=255)
                                                                                                                                                      tgGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGGTGGGGATACTGCTGCTGCGGCGGAAGCGGTTGTTGCGCGTGCTGATATTGCTGCTGCAGTGCATCCGGTTGTTGCGGGTGCTGTACATGt  >  7:1294106/1‑151 (MQ=21)
                                                                                                                                                      |                                                                                                                                                       
CGTCGGTTTATGCAACGGACGGCTGTCGCCATTACGCATCAACAGCGGATGAAGCAGGGTATCCTGCGGTTGTGGCGCAACTGGCTGTTGTGGCTGCTGATACTGCTGCTGCGGCGCAACCGGTTGTTGCGGCTGCTGATATTGCTGCTGTGGCGCAACCGGTTGTTGCGGCTGCTGATATTGTGGCTGCGGCGCAACCGGCTGTTGTGGCTGCTGATACTGCGGCTGCGGCGGAACTGGTTGTTGCGGCTGCTGATATTGCTGCTGCGGTGCAACCGGTTGTTGCGGCTGCTGTACAGGT  >  DH1/2945385‑2945685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: