Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,965,9060TG25.8% 175.7 / 26.0 144intergenic (+139/‑356)aqpZ/ybjEaquaporin/transporter
Reads supporting (aligned to +/- strand):  ref base T (13/82);  new base G (2/31);  total (29/115)
Fisher's exact test for biased strand distribution p-value = 3.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

AAGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCG  >  DH1/2965756‑2966054
                                                                                                                                                            |                                                                                                                                                    
aaGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAAt                                                                                                                                                      <  5:901548/151‑1 (MQ=255)
 aGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATc                                                                                                                                                     <  3:130494/151‑1 (MQ=255)
 aGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATc                                                                                                                                                     <  5:517655/151‑1 (MQ=255)
 aGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCT‑AGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATcg                                                                                                                                                    <  3:752354/151‑1 (MQ=255)
  ccGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATcg                                                                                                                                                    <  1:165321/150‑1 (MQ=255)
      gATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATcgccg                                                                                                                                                 <  8:579871/150‑1 (MQ=255)
      gATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATcgccg                                                                                                                                                 <  8:1310469/150‑1 (MQ=255)
       attaattaACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATcgccgcc                                                                                                                                               <  2:85624/151‑1 (MQ=255)
           attaaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGccc                                                                                                                                           <  4:823477/151‑1 (MQ=255)
              aaCGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGccccc                                                                                                                                         <  6:310964/150‑1 (MQ=255)
                cGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGc                                                                                                                                      <  1:328323/151‑1 (MQ=255)
                  cAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑GCG‑GGGGG‑GGCCCAAAACCGccccccccccccc                                                                                                                                    >  5:874907/1‑146 (MQ=255)
                   aGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CGGCGGGGG‑GGCCGTCCCCCGccccccccccccc                                                                                                                                    >  6:1223887/1‑146 (MQ=255)
                   aGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCAcc                                                                                                                                   <  2:253953/151‑1 (MQ=255)
                      aGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGCGG‑‑CCG‑GGGGG‑GGCCCACACCCCCCCCCCCCCGcccccc                                                                                                                                 >  8:1361149/1‑151 (MQ=255)
                      aGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCGCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCGCTGCAccccc                                                                                                                                <  8:449566/151‑1 (MQ=255)
                       ggCCTGGCGTATTGTCCGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCACGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCGTGCACCCCCg                                                                                                                               <  6:993791/151‑1 (MQ=255)
                        gCCTGGGGTAATGTGAGGCCTTTTTTTATGTTGTTGTTATATGTTGATTCATCATGTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TTTTGCCACTGGGGCACGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGGCGCGGCCCCCTGCACCCCCgg                                                                                                                              <  8:1029340/151‑1 (MQ=37)
                          cTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CCG‑GGGGG‑GGCCCAAAACCCCCCCCCCCCGCccccccccgc                                                                                                                            >  4:780345/1‑147 (MQ=255)
                              cGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGCGG‑‑CCG‑GGGGG‑GGCCAAAccccccccccccccccccccccccccccg                                                                                                                         >  4:277009/1‑144 (MQ=255)
                              cGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑GGC‑GGGGCGGGCCAAGCG‑CGCCCCCCCCCGCcccccccgccgcgg                                                                                                                        >  6:869753/1‑143 (MQ=255)
                                    gTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTGGCGCCCCTTCCATGAGCTAACCAACA‑TATTGCCACTGGCGCACGCCGCGC‑G‑‑CCG‑GGGGC‑GCCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAAc                                                                                                                  <  5:1059990/151‑1 (MQ=25)
                                      cggggttttttttATGTTTTTTTTATTTTTTGATTAATCATGTTGGGGGGCTCCTTTC‑TTCCCAAAACAAAATTTTTGCCAGGGGCGAATGGCGCGG‑G‑‑CTG‑GGGGC‑GCCAAAGCGCCGCCGCCCCGGGCACCCCCGGGCTCTGGCGAACaa                                                                                                                <  7:972923/145‑1 (MQ=2)
                                       aGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaaa                                                                                                               <  5:409698/151‑1 (MQ=255)
                                        ggCCTTTTTTTAATATTTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATTAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaaaa                                                                                                              <  6:579084/151‑1 (MQ=255)
                                        ggCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCGCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaaaa                                                                                                              <  6:146469/151‑1 (MQ=255)
                                         gCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCGTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAAt                                                                                                             <  1:717141/151‑1 (MQ=255)
                                         gCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAAt                                                                                                             <  3:780601/151‑1 (MQ=255)
                                         gCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGCAGCGC‑G‑‑CAG‑GGGGC‑GGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAAt                                                                                                             <  1:308382/151‑1 (MQ=255)
                                          ccTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATc                                                                                                            <  1:53262/151‑1 (MQ=255)
                                             ttttttAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑GGG‑GGGGG‑GGGCCACCGCCCCCCCCCCCCCCCCCCCCCCGCGCTGGcccccaaaaaaacc                                                                                                         >  1:939844/1‑138 (MQ=255)
                                              tttttagtttgttgtTATATGTTGCTTCATCATGTTGTTGGGCTCGTTTCTTGAGCTACGCAACA‑TATTGCCACTGGCGCAAGGCGCGG‑G‑‑CCG‑GGGGC‑GGCCAATCGCCGCCGCCCCGTGCACCCCCGGGCTCTGGCGAACAAAATcgcg                                                                                                         <  5:1009365/141‑2 (MQ=18)
                                              tttttAAGTTGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgcc                                                                                                         <  6:188991/150‑1 (MQ=255)
                                               ttttAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgccg                                                                                                        <  6:832534/150‑1 (MQ=255)
                                                 ttattttgttttTATTTTTTTATTCATATTTTTGTGCCGCTCTTTTCTTCCGATAACAAAAA‑TTTTCCCCGTGGCGAATGGCGCGC‑G‑‑CTG‑GGGGG‑GCCAAACCGCCGCCGCCCCGGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCt                                                                                                      <  2:1375576/144‑1 (MQ=2)
                                                 ttAAGATGTTGTTATATGTTGATTCATCATCTTGTGGCGCGCCTTTCATCAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCt                                                                                                      <  3:469641/150‑1 (MQ=255)
                                                   aatttgttgtTATATTTTGATTCATCATGTTGTTGCGCCCCTTTCTTGAGCTAAGCAACA‑TTTTCCCACTGGCGCATGGAGCGC‑G‑‑CTG‑GGGGG‑GCCCAATCGCCGCCGCCCCGTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTgc                                                                                                    <  8:126433/146‑1 (MQ=18)
                                                    aGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑GCCCGG‑GGGGC‑GCCAAACCCCCCCCCCCCCCCCCCCCCCCGCGCGC‑GCCGAACaaaaaacccccccac                                                                                                   >  4:122199/1‑140 (MQ=255)
                                                             tataTGTTCATTCATCATCTTGTGGCGCGCCTTGCATCAGCTAAGCAACA‑TATTCCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                          <  1:1185639/150‑1 (MQ=18)
                                                              ttatttgtatgcatcatCTTGTTGCGCTCCTTTCATGAGCTAACAAAAA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAAGCCCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTc                                                                                         <  5:1204951/139‑1 (MQ=18)
                                                              atatGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCGGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTc                                                                                         <  2:1263775/150‑1 (MQ=255)
                                                              atatGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTc                                                                                         <  1:751644/150‑1 (MQ=255)
                                                                atGTGGATTCATCATCTTGTTGCGCGCCTTTCCTGAGCTACGCAACA‑TATTGCCACTGGCGCATGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGAc                                                                                      <  6:341973/151‑1 (MQ=18)
                                                                  gTTCATTCATCATCTTGTTGCGCGCCTTTCATGAGTTAAGCAACT‑TATTGCCACTGGCGCATGGAGGGC‑G‑‑CTG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACtt                                                                                    <  6:1284124/151‑1 (MQ=18)
                                                                     gATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAAAA‑TATTGCCAGTGGCGCAAGGCGCGC‑G‑‑CTG‑GGGGC‑GGCAAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTAt                                                                                  <  4:370603/150‑1 (MQ=255)
                                                                      aTTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGG‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATc                                                                                 <  4:67177/150‑1 (MQ=255)
                                                                         catcatCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCtt                                                                             <  4:80469/151‑1 (MQ=255)
                                                                         catcatCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCtt                                                                             <  2:1188577/151‑1 (MQ=255)
                                                                         catcatCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCt                                                                              <  6:109130/150‑1 (MQ=255)
                                                                            aatcTTGTGGCGCTCCTTTCATGAGCTAACCAAAA‑TATTGCCAGTGGCGCAAGGCGCGC‑G‑‑CTG‑GGGGC‑GCCCAATCCCCGCCCCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTAc                                                                           <  5:767626/149‑1 (MQ=17)
                                                                              tcTTGTTGCGCGCCTTGCATGAGCTAAGCAAAA‑TATTCCCACGGGCGCAAGGCGCGC‑G‑‑CTG‑GGGGC‑GGCCAAGCGCCGCGGCCCCGTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACgg                                                                         <  7:1297497/150‑1 (MQ=17)
                                                                                ttgttgCGCTCCTTTCTTGAGATAAGCAAAA‑TATTGCCAGTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GCCCAAGCGCCGCGGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCt                                                                       <  7:780345/150‑1 (MQ=17)
                                                                                ttgtggCGCTCCTTTCATGAGATAAGCAAAA‑TATTGCCACGGGCGCACGGCGCGG‑G‑‑CCG‑GGGGG‑GGCCAAGCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCt                                                                       <  7:113568/150‑1 (MQ=17)
                                                                                   ttgCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑GCG‑GGGGC‑GGGCCCGCGCCCCCGCCCCCCCCCCCCCCCGGCGCGGGCGCCCAAAAAACCCCCGGCGGGGGGCCCCCCCCATATCCCCTACGGCTcccc                                                                   >  3:519324/1‑150 (MQ=255)
                                                                                    ggcgcTCCTTTCATGAGCAAAGCAACA‑TATTGCCACTGGCGCAAGGCGCGC‑G‑‑CAG‑GGGGC‑GGCCAAGCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCgg                                                                  <  2:172124/150‑1 (MQ=255)
                                                                                     gcgcTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCggg                                                                 <  6:653857/151‑1 (MQ=255)
                                                                                     gcgcTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCgg                                                                  <  5:457321/150‑1 (MQ=255)
                                                                                      cgcTCGTTTCATGACCTAAGCAACA‑TATTGCCACTGGCGCAAGGCGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGt                                                                <  8:1018185/151‑1 (MQ=255)
                                                                                      cgcTCCTTTCATGCGCTAACCAACA‑TATGGCCACTGCCGCAAGGAGCGC‑G‑‑CCG‑GGGGC‑GGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGt                                                                <  3:1145159/151‑1 (MQ=17)
                                                                                             ttCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGgcg                                                          <  4:124546/150‑1 (MQ=255)
                                                                                             ttCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGgc                                                           <  4:744398/149‑1 (MQ=255)
                                                                                              tCATGAGCTACCCAACA‑TATTGCCACTGGCGCAAGCAGCGC‑G‑‑CAG‑GGGGC‑GGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCGCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGgcgc                                                         <  2:50720/150‑1 (MQ=255)
                                                                                               cgtgtcgttaGCCACA‑TTTTCCAACTGGGGCACGCACCGG‑G‑‑CAG‑GGGGC‑GCCCTAGCCGCGCCGCCGGGTGCACCCGCGGGCTCTGGCGAACAAACTCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGgggcgggc                                                           <  5:460103/138‑1 (MQ=2)
                                                                                                 tGACCTAACAAAAA‑TTTTGCCACGGGCGAACGGCGCGG‑G‑‑CTG‑GGGGC‑GGCAAAGCGCCGCCGCCCCGTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                                       <  8:45609/149‑1 (MQ=11)
                                                                                                  gAGCTAAGCAACA‑TATTGCCACTGGCGCACGGAGCGC‑G‑‑CCG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                                    <  1:864691/151‑1 (MQ=255)
                                                                                                  gAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                                     <  1:718513/150‑1 (MQ=255)
                                                                                                    gCTAAGCAACA‑TATTGCCACTGGCGCAAGGCGCGC‑G‑‑CCG‑GGGGC‑GGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                  <  5:591881/151‑1 (MQ=255)
                                                                                                          aaaCA‑TATTGCCACTGGCGCACGGCGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATccc                                            <  8:84943/150‑1 (MQ=255)
                                                                                                           aaCA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                                           <  2:273055/151‑1 (MQ=39)
                                                                                                           aaCA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                                           <  4:303071/151‑1 (MQ=39)
                                                                                                            aCA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTg                                          <  7:451535/151‑1 (MQ=38)
                                                                                                             aa‑tatTGCCACTGGCGCAAGGAGCGC‑G‑‑CTG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt                                         <  4:450807/150‑1 (MQ=37)
                                                                                                                tatTGCCCCTGGGGGAAGGAGCGC‑G‑‑CTG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTa                                        <  5:591385/150‑1 (MQ=255)
                                                                                                                 atTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaaa                                      <  2:249691/151‑1 (MQ=35)
                                                                                                                    gCCAGTGGCGCAAGGAGCGC‑G‑‑CTG‑GGGGG‑GGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                   <  3:440848/151‑1 (MQ=255)
                                                                                                                    gCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                   <  4:529878/151‑1 (MQ=33)
                                                                                                                            cgcACGGAGCGG‑G‑‑CAG‑GGGGC‑GGCAAAGCCCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCa                           <  7:213604/151‑1 (MQ=255)
                                                                                                                             gcACGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg                          <  3:928525/151‑1 (MQ=14)
                                                                                                                              cacggcgcgc‑g‑‑cGG‑GGGGC‑GGCCAAGCGCGGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                         <  8:842984/145‑1 (MQ=11)
                                                                                                                               aaGGCGCGC‑G‑‑CCG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                        <  2:599489/151‑1 (MQ=14)
                                                                                                                                    gcgc‑g‑‑cAG‑GGGGC‑GGCCAAGCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAt                   <  7:232279/151‑1 (MQ=14)
                                                                                                                                     cgc‑g‑‑cAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc                  >  8:1357334/1‑151 (MQ=31)
                                                                                                                                      gc‑g‑‑cAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc                 <  3:1173557/151‑1 (MQ=31)
                                                                                                                                       c‑g‑‑cgG‑GGGGC‑GGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                <  6:951248/151‑1 (MQ=14)
                                                                                                                                            cAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg              >  3:580830/1‑151 (MQ=31)
                                                                                                                                            cAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg              >  8:1169545/1‑151 (MQ=31)
                                                                                                                                             aG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgt             >  5:320913/1‑151 (MQ=31)
                                                                                                                                             aG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtct             >  4:793584/1‑149 (MQ=14)
                                                                                                                                              g‑ggggC‑GGCCAAGCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg            <  1:1360930/151‑1 (MQ=14)
                                                                                                                                                ggggC‑GCCCACGCGCCCCCCCCCCGGCCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTgg           <  8:1323019/151‑1 (MQ=11)
                                                                                                                                                 gggC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg          >  1:376721/1‑151 (MQ=31)
                                                                                                                                                    c‑ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg       <  3:1244113/151‑1 (MQ=31)
                                                                                                                                                      ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGc      >  5:38220/1‑151 (MQ=31)
                                                                                                                                                      ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGc      <  1:746945/151‑1 (MQ=31)
                                                                                                                                                      ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGc      >  5:118307/1‑151 (MQ=31)
                                                                                                                                                        ccAATCGCCGCCGCCCCATGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGccc    <  5:519852/151‑1 (MQ=14)
                                                                                                                                                        ccAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGccc    <  2:968447/151‑1 (MQ=14)
                                                                                                                                                         cAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc   <  5:792720/151‑1 (MQ=31)
                                                                                                                                                          aaTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCg  >  3:344955/1‑151 (MQ=31)
                                                                                                                                                          aaTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCg  <  8:580051/151‑1 (MQ=31)
                                                                                                                                                            |                                                                                                                                                    
AAGCGTGATTAATTAACGCAGAAGGCCTGGCGTAATGTCAGGCCTTTTTTTAAGATGTTGTTATATGTTGATTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAGCAACA‑TATTGCCACTGGCGCAAGGAGCGC‑G‑‑CAG‑GGGGC‑GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGACTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCG  >  DH1/2965756‑2966054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑