Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,122,1240CA25.6% 320.5 / 17.8 211intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (87/41);  new base A (41/3);  total (165/46)
Fisher's exact test for biased strand distribution p-value = 5.75e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

CTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121975‑4122272
                                                                                                                                                      |                                                                                                                                                     
      cAACCGCGCTATTCCCGCTTGGGTGGTGTGTCCCTTACATCACTTGCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTgcag                                                                                                                                               <  7:471439/151‑1 (MQ=17)
       aaCTTACCTATTCCCTCTTGTGTTGTCTGTGAGTTAAATCTCTTTAAAATTTTGGAGCGGGAAACGAGAGTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTgcagc                                                                                                                                              <  8:65166/151‑1 (MQ=14)
         cTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTAGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAATGAGCTATTCCCGCGTGGGTCGTCAG‑TTGT‑TTgcagca                                                                                                                                             >  5:582700/1‑150 (MQ=14)
           caGCTATTACGGCTTGGTTGGTCTGTCCGTTCCTGTACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACtt                                                                                                                                          <  8:737671/150‑1 (MQ=17)
               tATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGTTGGTCTG‑TTGT‑GTGTAGGACTTTTcc                                                                                                                                      >  5:833113/1‑150 (MQ=14)
               tATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCTT‑TGGT‑TTgccccacgtgtg                                                                                                                                       >  3:1269141/1‑140 (MQ=14)
                atacgcgagtGGGTGGTCTGTGCCTTAAAGAACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCaa                                                                                                                                     <  5:174053/142‑1 (MQ=255)
                aatcccctttGGGTGGTCTGTTAATTACATCAATTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCaa                                                                                                                                     <  4:211630/143‑1 (MQ=17)
                 atcgctctgggtcgctCTGTGTCTTACATAACTTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCaaa                                                                                                                                    <  4:746877/136‑1 (MQ=17)
                  tCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTG‑TGCT‑TTGCAGCACTGTTCaatt                                                                                                                                   >  5:1093038/1‑149 (MQ=255)
                  tCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTATT‑TTAT‑TTGTAGGACTTTTCGAAt                                                                                                                                   >  7:1275148/1‑151 (MQ=14)
                   cccGCGGGGGCGGTCTGTGGCTTAGATCACTATAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAAtt                                                                                                                                  <  3:414566/151‑1 (MQ=17)
                      gCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTCGTATG‑TTAT‑GTGGAGGACTTTTCCAAttttt                                                                                                                               >  5:940306/1‑150 (MQ=14)
                      gCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCTG‑TGCG‑TTTCCGCACTTTTCTAAttttt                                                                                                                               >  7:105045/1‑150 (MQ=17)
                       cTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTT‑TTGT‑TTGCAGCACTTTTCAAATTTTgg                                                                                                                              >  7:317966/1‑151 (MQ=255)
                       cTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCTG‑TTAG‑TTTACGGAATTTTCAAATTTTgg                                                                                                                              >  6:1173388/1‑151 (MQ=14)
                       cTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGAGGTCTG‑TGAT‑ATTCAGAAATTTTCAAAtttttg                                                                                                                              >  6:67353/1‑149 (MQ=14)
                        ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTgg                                                                                                                              >  7:518424/1‑150 (MQ=255)
                        ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGTGGTCTT‑TGGT‑TTGCCGGACTTTTCAAATTTTgg                                                                                                                              >  3:673999/1‑150 (MQ=255)
                        ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGTACGTCTG‑TTAT‑TTTGAGAAATTTTCAAAtttttgc                                                                                                                             >  6:1065877/1‑148 (MQ=14)
                        gggggggctctggagcTTAAATCACTTTCAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGa                                                                                                                             <  3:117563/136‑1 (MQ=14)
                         tGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTGGTATG‑TGAT‑TTTAAGCAATTTTCAAATTTTgg                                                                                                                              >  8:781444/1‑149 (MQ=17)
                         tGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTG‑TGGT‑TTGGAGGACTTTTCAAATTTTgg                                                                                                                              >  1:448469/1‑149 (MQ=255)
                         tGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTACCGCTTGGGTGTTATT‑TTTT‑TTGGAGGAATTTTCAAATTTTgg                                                                                                                              >  2:179583/1‑149 (MQ=17)
                          ggtgggtgtgttacTTAAAGTACTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGc                                                                                                                           <  8:31202/138‑1 (MQ=14)
                             tggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTG‑TGGG‑GTGGCGGACTTTTCAAATTTTGGCGCggg                                                                                                                        >  3:547074/1‑151 (MQ=17)
                              ggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGGG‑GTGGAGCACTTTTCAAATTTTGGAGCgggt                                                                                                                       >  1:1340565/1‑150 (MQ=255)
                                tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAAt                                                                                                                                   >  1:144855/1‑137 (MQ=255)
                                 gtgttcattACATCCCTATAAAATTTTGGAGCGGTAAACGATACTCGAATTTTCTACCCCTACCTTTGCAAGGTAGTGCTATAAAAAATGAGCTATTCCGGATTGGGTGGTCTG‑TGAT‑TTTCATCACTTTTCAAATTTTGGAGCGGGaaa                                                                                                                     <  2:578874/143‑1 (MQ=9)
                                  tgtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATG‑TTAT‑TTGCAGGAATTTTCTAATTTTGGAGCGGTAAACg                                                                                                                   >  7:659806/1‑151 (MQ=18)
                                  tgtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGAGGTCTG‑TTAG‑TTGGCGGAATTTTCTAATTTTTGAGTGGGAACCg                                                                                                                   >  3:50981/1‑151 (MQ=14)
                                    tgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCTACGTCTT‑TTAT‑TTGAAGAAATTTTCAAATTTTTGAGAGGGAAACg                                                                                                                   >  6:57755/1‑149 (MQ=17)
                                     gCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑GTGCAGCACTTTTCAAATTTTGGAGCGGGAACCgagc                                                                                                                >  7:722362/1‑150 (MQ=255)
                                       cTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTCTG‑TGAT‑TTGGAGCACTTTTCAAATTTTTGAGCGGGAAACgagccg                                                                                                              >  5:1224300/1‑148 (MQ=255)
                                       cTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGTTCGTATG‑TTTTGTTGC‑GGAATTTTCAAATTTATGAGGGGGAACCgaagcg                                                                                                              >  4:1307440/1‑147 (MQ=11)
                                         tACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGAGGTCAT‑TTGT‑ATGAAGCACTTTTCACATTTTTGAGGGGTAAACgcgccggg                                                                                                            >  5:436665/1‑144 (MQ=14)
                                         tACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCACGCTTGGGTGGTCTG‑TTAT‑TTTCAGTAATTTTCTAATTTTTGAGAGGGAAACGCGACTcc                                                                                                            >  1:80050/1‑150 (MQ=17)
                                           cAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTAGGTGGTATG‑TTAT‑ATGACGAAATTTTCAAATTTTGGAGACGTAAACgccacacca                                                                                                           >  2:1256897/1‑142 (MQ=14)
                                            tttattttaatcttttGGAGCGGTAAACGAGACTCCAAGTCGCTACCCCTATCTTGGCAATGTCGTGGTCTACGATCTGAGCTATTCCCGCTTGGGTGGTCTG‑TGAT‑TTTTATTACTTTTAATATTTTGGAGCGGGAAACGAGACTCGaa                                                                                                          <  6:387229/138‑1 (MQ=2)
                                             gCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGTACGTATG‑TTGT‑GTGAAGAAATTTTCAAATTTATGAGAGGGAAACggcccacga                                                                                                           >  8:1127020/1‑140 (MQ=14)
                                              cACTTTCAAATTTTGGAGTGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑GTGGAGCACTTTTCAAATTTTGGAGAGGGAAACGCGCCTCGAACTc                                                                                                       >  1:187778/1‑151 (MQ=14)
                                                cTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTCGTATG‑TGAT‑TTTAAGCACTTTTCAAATTTTTGAGAGGGTACGGCGACTCCATCTcgc                                                                                                     >  6:1006009/1‑151 (MQ=14)
                                                atttCAATTTTTGTACCGGCAAACTTGACTAGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTcgc                                                                                                     <  8:6221/150‑1 (MQ=14)
                                                   tCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTG‑TGAT‑GTTCAGGACTTTTCAAATTTTGGAGCGGGAAACGAGCCGCGATCTCGCGAc                                                                                                  >  1:931787/1‑151 (MQ=14)
                                                    aaaaTTTTGGTGAGGGACAGGGGAGGGGAACTCGGGACCCCGACCTTGTGTCTGTCGTGCACTACCAACTGCGCTATTCCCGTTGGGGGGGTCTG‑TCCC‑TTACATCAC‑TTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGAccc                                                                                                <  5:1186325/150‑1 (MQ=2)
                                                        ttttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TTTT‑TTTAAGAAATTTTCAAATTTTTGAGACGGAAACGCGACTCGAACTCGCGAcaa                                                                                                >  4:1013193/1‑146 (MQ=11)
                                                                cGGGAAACGTGCCGCGAACCCGCGACCCCGACCTTGTCAAGGTCGTGCTCTACCAACTGCGCTATTCCCGCTTGGGTGGTCTG‑TGGT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCa                                                                                     <  7:996722/151‑1 (MQ=14)
                                                                cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCCGCACTTTTCAAATTTTGGAGCGGGAAACGCGCCTCGAACTCGCGACCCCGCCCTTCGc                                                                                      >  7:397390/1‑150 (MQ=255)
                                                                cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTG‑TGCT‑GTTGAGCACTTTTCTAATTTTTGAGCGGGAAACGAGACTCGAACTCTCGACCCCGAACTTggg                                                                                      >  6:1121369/1‑149 (MQ=11)
                                                                  ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCCGTGGTCAG‑TTGT‑ATGAAGGAATTTTCGAATTTTGGATAGGGAAACTAGACACGATCTATCGACCACTACATTggaa                                                                                     >  8:25249/1‑147 (MQ=2)
                                                                   gAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGTCGTCTC‑AGCT‑TTGCAGCTCTTTTCAAATTTTGGAGCGGGATACGAGCCTCGCACTCGCGACCCCGACCTTGGCAAgg                                                                                  >  3:342205/1‑151 (MQ=14)
                                                                    aaaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGTTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTTGAGAGGGAAAGGAGACTCGAACTCGCGACCAAGACGTTGGCAAGGt                                                                                 >  8:363654/1‑151 (MQ=14)
                                                                     aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCTAATTTTGGAGAGGGAAACGAGACTCGAACTCGCGACCCCGCCCTAGGCAAGCTc                                                                                >  4:442280/1‑151 (MQ=255)
                                                                       cGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGGTCTG‑TTGT‑TTGCAGGAATTTTCAAATTTAGGAGAGGGAACCGCGACTCCAAATAGCGACCCCGACCTTCGCAAGCTCgg                                                                              >  4:262266/1‑150 (MQ=14)
                                                                       cGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTATG‑TGCT‑ATGCAGCACTTTTCAAATTTTGGAGAGGGAACCGAGACTCGAACTCGCGACCCCGCCATTCCCAAGGACGt                                                                              >  2:281182/1‑151 (MQ=14)
                                                                         agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCTTGTTATG‑TGAT‑GTTGAGGACTTTTCAAATTTTGGAGTGGTTACCGCGGGTCGATCTGGCGAGACCGCGCTTGGTATGGTCGTGc                                                                            >  1:159469/1‑151 (MQ=2)
                                                                            cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATT‑TGAT‑TTTAAGCACTTTTCAAATTTTTGAGAGGGAAACGAGACTCGAACTAGCGACACAGACATTGGCAAGGtagacctc                                                                          >  8:1352814/1‑143 (MQ=11)
                                                                               gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGGT‑TTGGAGCACTTTTCGAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCGCTAc                                                                       >  5:100072/1‑150 (MQ=255)
                                                                                aaCTCGCGAGCCCGACCTTGGAATGGTGTTGCTCTTCCACCTGAGCGATTCCCGCTTGGGTGGTCTG‑TGCC‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCa                                                                     <  1:1255410/151‑1 (MQ=11)
                                                                                 aCTCGCGACCCCGACCTTGGCAAGGTCTTGCTCTACCAACTGACCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTTCATCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCaa                                                                    <  8:1211870/151‑1 (MQ=255)
                                                                                   tCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                  >  7:101262/1‑151 (MQ=38)
                                                                                   ccgcgCCCCCGCCCGTGGCCAGGGCGTGCTCTACCATTTTTTTTATTACCCGTTGGGTTGTGTG‑TTCT‑ATTCAGGACTTTTCAATTTTTGGATCGTGTAAGGTGTTTCGATCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                  <  2:1275965/150‑1 (MQ=2)
                                                                                       gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTCGTCTG‑TGAT‑TTGCAGAACTTTTCAAATTTATGAGCGGGAAACGAGACTCGAACTAGCGACACCTCACTAGCAAAGCTCGAGCGCTACAAACTg                                                                 >  2:840661/1‑148 (MQ=14)
                                                                                       caCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGACGTCTG‑TTTG‑TTGGAGAAATTTTCAAATTTAGGATCCGGAACCGACACGCTATCTCCAGAACACACCCGAGGCAAGGTCGTCATCTACCTACTg                                                                 >  5:775233/2‑148 (MQ=2)
                                                                                        aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATG‑TGGT‑TTGCAGCAATTTTCAAATTTTGGAGCGGGAAACGCGACTCGAACTCGCGACCACGCCCTTGGCAAGGTCGTGCTCTACAAACTGAGCt                                                             >  1:65618/1‑151 (MQ=14)
                                                                                            cggccTTGGCCTGCTGGTGCTCTAGCACGTGCGGTTTCCCCTCTTGGGTGGTCTG‑TGCC‑TTGCAGCAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTc                                                         <  5:375771/148‑1 (MQ=11)
                                                                                             gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTcc                                                        >  3:455734/1‑151 (MQ=38)
                                                                                                cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGAT‑TTTCAGAACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAAATAGCGACCCAGCGCTTGGGAAGGTCGTGCTCTAACAACTGAGGTATTACcgg                                                     >  8:861195/1‑150 (MQ=11)
                                                                                                 ttGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCTTCTTAAGTTGGG‑TTG‑AGAAATTTTCAAATTTTTGAGAGGTAAACGACACGCGATGTCGCGAGAACGACCTTCGAAAGGTACTGCACTAAAAACTGATATATTccgt                                                      >  3:756084/1‑147 (MQ=2)
                                                                                                       aGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGAGGTAAACGAGACTCGAAATCGCGACACCGACATGGGGAAGGTCGTGCTCTAAAAACTGAGCTATTCACGCGtacta                                               >  1:1371553/1‑150 (MQ=14)
                                                                                                          tCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑GTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTAGCGACACCGAGCTGGCCAAGGTCGTCCTCTACAAACTGAGCTATTCCCGCGTACTACtt                                            >  3:383997/1‑150 (MQ=14)
                                                                                                           cgtccgcccccAACGGAGCTCTACGCGCTTGCGTGGTGTG‑CCCT‑GGGGAGCTCTTCCGAACTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTaa                                          <  3:236545/142‑1 (MQ=14)
                                                                                                             tGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGGT‑TTGGAGCACTTTTCAAATTTTTGAGCGGGAAACGAGACTCGAAATCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCAGCGTACTACTTAAtt                                        >  5:297733/1‑151 (MQ=255)
                                                                                                             tGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAtt                                        >  8:76716/1‑151 (MQ=255)
                                                                                                             tGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAtt                                        >  7:218963/1‑151 (MQ=255)
                                                                                                               ctctACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAtttt                                      >  3:891116/1‑151 (MQ=255)
                                                                                                                tctACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                     >  7:49563/1‑151 (MQ=255)
                                                                                                                tctACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTCCTCTACCAACTGAGCTATTCCCGCGTACTACGTAAGTTTg                                     >  2:759366/1‑151 (MQ=255)
                                                                                                                 ctACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGc                                    >  4:674553/1‑151 (MQ=255)
                                                                                                                   aCCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCtt                                  >  6:35127/1‑151 (MQ=255)
                                                                                                                    ccAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCttt                                 >  3:495466/1‑151 (MQ=255)
                                                                                                                    ccAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACATAATTTTGAttt                                 >  6:479274/1‑151 (MQ=255)
                                                                                                                      aaCTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCg                               >  4:955372/1‑151 (MQ=255)
                                                                                                                      aaCTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCg                               >  2:144879/1‑151 (MQ=255)
                                                                                                                        cTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTctt                              >  3:844282/1‑148 (MQ=255)
                                                                                                                        cTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                              >  1:1303545/1‑150 (MQ=255)
                                                                                                                         tGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTa                             >  8:553863/1‑150 (MQ=255)
                                                                                                                                 tCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  5:1384754/1‑150 (MQ=255)
                                                                                                                                  cccGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAt                   >  2:100418/1‑151 (MQ=255)
                                                                                                                                   ccGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAtt                  >  5:498654/1‑151 (MQ=255)
                                                                                                                                    cGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAttt                 >  1:1138816/1‑151 (MQ=255)
                                                                                                                                     gCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGACCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTc                >  2:388908/1‑151 (MQ=255)
                                                                                                                                      cTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCt               >  4:670485/1‑151 (MQ=255)
                                                                                                                                      cTTGGGTGGTCTG‑TGCC‑TTACAGCAC‑TTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCTTCTTATTTTTTATTTAGGAATTTTCTAATTTTTg              >  6:785929/1‑151 (MQ=11)
                                                                                                                                       ttGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTg              >  6:1247410/1‑151 (MQ=255)
                                                                                                                                        tGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGc             >  8:755653/1‑151 (MQ=255)
                                                                                                                                             ggtCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcgt        >  7:1163367/1‑151 (MQ=255)
                                                                                                                                              gtCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt       >  2:943267/1‑151 (MQ=255)
                                                                                                                                               tCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt       >  5:661340/1‑150 (MQ=255)
                                                                                                                                                      cT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  2:346595/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTG‑TGCT‑TTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121975‑4122272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑