Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 3,668,924 (T)5→6 pseudogene (353/455 nt) lpxA ← hydroxymyristoyl‑ACP dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,668,9191.T98.3% 865.5 / 4.0 236pseudogene (358/455 nt)lpxAhydroxymyristoyl‑ACP dehydratase
Reads supporting (aligned to +/- strand):  ref base . (3/1);  new base T (101/131);  total (104/132)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT  >  DH1/3668769‑3669064
                                                                                                                                                        |                                                                                                                                                  
cccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGcgcg                                                                                                                                                     <  4:632977/151‑1 (MQ=255)
cccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGCTCAGGCCGCGGcgcg                                                                                                                                                     >  8:797367/1‑151 (MQ=255)
 ccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGt                                                                                                                                                   <  2:627774/151‑1 (MQ=255)
  ctctTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtt                                                                                                                                                 >  3:465904/1‑151 (MQ=255)
    ctTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtt                                                                                                                                                 <  3:693282/149‑1 (MQ=255)
      tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtttttt                                                                                                                                             >  4:239928/1‑150 (MQ=255)
      tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtttt                                                                                                                                               <  2:709557/149‑1 (MQ=255)
       ccACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtttttt                                                                                                                                             <  7:632519/150‑2 (MQ=255)
       ccACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGtttttt                                                                                                                                             <  8:508131/150‑2 (MQ=255)
        cacaATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcg                                                                                                                                           >  6:702177/1‑148 (MQ=255)
         acaATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcga                                                                                                                                          <  6:3717/151‑5 (MQ=255)
          caATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcgaa                                                                                                                                         <  1:591983/151‑6 (MQ=255)
          caATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcgaa                                                                                                                                         <  1:596988/151‑6 (MQ=255)
          caATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGttttttcgaa                                                                                                                                         <  5:421165/151‑6 (MQ=255)
              ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGGTTTTTCGAAAGTg                                                                                                                                      >  2:816043/1‑151 (MQ=255)
                cGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGAc                                                                                                                                    >  8:286899/1‑151 (MQ=255)
                    tGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTcc                                                                                                                                >  1:872248/1‑151 (MQ=255)
                    tGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCCTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTc                                                                                                                                 <  3:574870/150‑1 (MQ=255)
                     ggctGCACAAAGGCGGATTTATCAATGACGTATCAGGCCTCCCGTCTACGAGCACACATCATCGTTGGTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCa                                                                                                                               <  6:271697/148‑1 (MQ=255)
                        tGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATg                                                                                                                             >  5:388268/1‑150 (MQ=255)
                          cacaAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTGCATGAtg                                                                                                                          >  8:221537/1‑150 (MQ=255)
                          cacaAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATc                                                                                                                          >  4:760860/1‑151 (MQ=255)
                           acaAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATc                                                                                                                          >  5:652465/1‑150 (MQ=255)
                           acaAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCa                                                                                                                         <  6:696608/151‑1 (MQ=255)
                             aaaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCAtt                                                                                                                       <  2:739806/151‑1 (MQ=255)
                                  cGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGCGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTGTTTTCGAAAGTGACTTCCATGAGCATTTGATc                                                                                                                  >  4:655600/1‑151 (MQ=255)
                                   ggATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCg                                                                                                                 <  1:896837/151‑1 (MQ=255)
                                    gATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGc                                                                                                                <  3:50358/151‑1 (MQ=255)
                                           caatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCAc                                                                                                         <  3:535980/151‑1 (MQ=255)
                                            aatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACg                                                                                                        <  3:167504/151‑1 (MQ=255)
                                             atcaCGTATCGGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGa                                                                                                       <  2:172050/151‑1 (MQ=255)
                                               caCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGAcc                                                                                                     <  8:761533/151‑1 (MQ=255)
                                               caCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGAc                                                                                                      <  4:982564/150‑1 (MQ=255)
                                                 cGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCgg                                                                                                   >  5:267896/1‑151 (MQ=255)
                                                    aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcg                                                                                                >  2:968858/1‑151 (MQ=255)
                                                    aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcg                                                                                                >  5:512253/1‑151 (MQ=255)
                                                    aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcg                                                                                                <  5:611351/151‑1 (MQ=255)
                                                    aTCAGGCATCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGGCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgc                                                                                                 <  7:828980/150‑1 (MQ=255)
                                                     tCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcgc                                                                                               >  4:182771/1‑151 (MQ=255)
                                                          ccTCCCGGCGACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGaa                                                                                          <  1:1005810/151‑1 (MQ=255)
                                                            tCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgc                                                                                        >  7:890417/1‑151 (MQ=255)
                                                              ccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgc                                                                                      <  4:301781/151‑1 (MQ=255)
                                                                ggCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgcgc                                                                                    >  1:495436/1‑151 (MQ=255)
                                                                 gCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCt                                                                                   <  8:1062810/151‑1 (MQ=255)
                                                                  cTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCtt                                                                                  >  8:496698/1‑151 (MQ=255)
                                                                      gAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAATCGCGCTTCGTc                                                                              <  5:234335/151‑1 (MQ=255)
                                                                      gAGCACACATCATCGTTGCTTCGCAAACTACATTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTc                                                                              <  3:32828/151‑1 (MQ=255)
                                                                       aGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTc                                                                              >  5:82005/1‑150 (MQ=255)
                                                                       aGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCa                                                                             <  7:287124/151‑1 (MQ=255)
                                                                         cacacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCTCTTGAAGCGCGCTTCGTCAAt                                                                           >  5:160335/1‑151 (MQ=255)
                                                                         cacacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAAt                                                                           >  2:415915/1‑151 (MQ=255)
                                                                         cacacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGACCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAAt                                                                           >  6:22643/1‑151 (MQ=255)
                                                                           cacaTCATCTTTTCTTAGCAAACTACTTTACCATCTACCAGAGCAAGCCCTTTATAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTTACTTCCATGATCATTTGATCTCCAGGCACGACCGTGCGCTTGAAGCGCGCTTCTTCAATAc                                                                         <  8:463238/151‑1 (MQ=255)
                                                                             catcatCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCGTTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCa                                                                       <  7:167340/151‑1 (MQ=255)
                                                                             catcatCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATAccc                                                                       >  1:166396/1‑150 (MQ=255)
                                                                                   cGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAag                                                                 <  5:690861/151‑1 (MQ=255)
                                                                                    gtcgCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagt                                                                >  6:1064399/4‑151 (MQ=255)
                                                                                      tGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagtat                                                              >  2:668247/1‑150 (MQ=255)
                                                                                      tGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagta                                                               <  7:806925/150‑1 (MQ=255)
                                                                                      tGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagta                                                               <  4:506768/150‑1 (MQ=255)
                                                                                      tGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagta                                                               <  7:506246/150‑1 (MQ=255)
                                                                                       gCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagtagt                                                             <  3:758819/151‑1 (MQ=255)
                                                                                         ttCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAc                                                           >  4:743135/1‑151 (MQ=255)
                                                                                          tCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAgtagta                                                            <  6:777039/149‑1 (MQ=255)
                                                                                           cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACa                                                          <  1:947132/150‑1 (MQ=255)
                                                                                           cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACa                                                          <  7:459868/150‑1 (MQ=255)
                                                                                           cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACa                                                          <  8:703335/150‑1 (MQ=255)
                                                                                           cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAg                                                         <  7:1080429/151‑1 (MQ=255)
                                                                                             cAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCt                                                       <  1:1030582/151‑1 (MQ=255)
                                                                                               aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTGGAAAGTGACTTACATGATCAATTGATCGCCCGGCACGACCGGGCGCTTGAAGCGCGCGTCGTGCATAGCAGCCAAgtagtaaacctg                                                      >  8:958248/1‑144 (MQ=255)
                                                                                                   actTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCg                                                 <  5:349821/151‑1 (MQ=255)
                                                                                                    ctTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCgg                                                >  2:581215/1‑151 (MQ=255)
                                                                                                     tttACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCATCGTCAATACCAGCGAAGTAGTACAGCTCACCCggg                                               >  2:347034/1‑150 (MQ=255)
                                                                                                       tACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGtt                                              <  4:114770/150‑1 (MQ=255)
                                                                                                       tACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGtt                                              <  6:649920/150‑1 (MQ=255)
                                                                                                        aCCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTc                                             <  6:261728/150‑1 (MQ=255)
                                                                                                         ccctCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTGCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCa                                           <  6:574336/148‑1 (MQ=255)
                                                                                                         ccATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCa                                           <  5:1079508/151‑1 (MQ=255)
                                                                                                          cATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCAGCGGCGAGTTTTTTCGAAAGTGCCTTCCATTATCATTTGATCGCAAGGCCAGACCGGGCGCTTGAAGCGCGATTCGTCAATAACAGCTAAGTAGTAAAGCTCAACCGGttac                                            >  3:731471/1‑147 (MQ=255)
                                                                                                             cGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCACttt                                       >  4:604970/1‑151 (MQ=255)
                                                                                                                 cAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCt                                   >  5:711981/1‑151 (MQ=255)
                                                                                                                  agagCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCt                                   >  2:1011066/1‑150 (MQ=255)
                                                                                                                  agagCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCt                                   >  1:289296/1‑150 (MQ=255)
                                                                                                                   gagCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTAc                                 <  5:1056406/151‑1 (MQ=255)
                                                                                                                   gagCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTAc                                 >  5:609162/1‑151 (MQ=255)
                                                                                                                        aCCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCttt                            <  3:893135/151‑1 (MQ=255)
                                                                                                                         ccccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtttt                           <  3:131855/151‑1 (MQ=255)
                                                                                                                         ccccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtttt                           <  4:314045/151‑1 (MQ=255)
                                                                                                                         ccccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtttt                           <  1:485215/151‑1 (MQ=255)
                                                                                                                           ccTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTa                          >  2:51451/1‑150 (MQ=255)
                                                                                                                              ttAAAACGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAAc                       <  3:1013353/150‑1 (MQ=255)
                                                                                                                                aaaaCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGcc                    >  2:850579/1‑151 (MQ=255)
                                                                                                                                aaaaCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGcc                    >  6:476598/1‑151 (MQ=255)
                                                                                                                                aaaaCGGGTCAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGc                     <  1:741840/150‑1 (MQ=255)
                                                                                                                                         cAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCt           <  7:757720/151‑1 (MQ=255)
                                                                                                                                         cAGGCCGCGGCGCGTTTTTTCGAAAGTGACTTACATGATCATTTGATCGCCACGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAAAACCACCGAAGTATGACAGCACACCCGGTTCCAGTTTTCCTAAGCTTTTAAACGCCAGAATAACt           >  7:250456/1‑151 (MQ=255)
                                                                                                                                          aGGCCGCGGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTg          >  6:1004592/1‑151 (MQ=255)
                                                                                                                                              cgcgGCGCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGc      >  2:978090/1‑151 (MQ=255)
                                                                                                                                                 ggcgCGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtg   <  6:901563/151‑1 (MQ=255)
                                                                                                                                                  gcgcGTTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtgt  <  1:983499/151‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                  
CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGTTTTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT  >  DH1/3668769‑3669064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: