Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA28.0% 149.5 / 10.5 117intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (55/17);  new base A (28/0);  total (99/18)
Fisher's exact test for biased strand distribution p-value = 2.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCA  >  DH1/613623‑613920
                                                                                                                                                    |                                                                                                                                                      
tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTG‑GAGGGGCAGAAAGATGAATGACTGTACAACCCAATATCCACAAAAAAAAGAAGATTATctttcactttcaac                                                                                                                                                     >  8:97375/1‑149 (MQ=11)
             ccccgtcTTATCGGGCAGTATCACCTGCTATACGTAAATCGCCGGATGGCCTGGTTTTTGGTTGTG‑GTGGCTCCCTATGATGAAGGCCTCGCCCAGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTaaa                                                                                                                                        <  3:97619/145‑1 (MQ=2)
             acccGGATTATGGGGCAGTATCCCCTCGTTTCGGTAACCCCGGGCTTCGGCGGGTTTTTGGTTTTG‑AGGGGGCTAAAACATGATTGCCTGGCCACCACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTaaa                                                                                                                                        <  3:314651/148‑1 (MQ=2)
              agCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGATTCGGCGGGTTTTTGCTTTTG‑GAGTGGCAGATAGATGAATGACTTTCCAAGAAACTATGCCCAAAAGAAAGCCGGTTATCGGTAAGTTTGACGTGGTTTACGTaaa                                                                                                                                        >  7:411982/1‑150 (MQ=11)
                 ggCTTATTTTTCAGTTGCCCCTGGTTTACG‑AAAACCCGGCCCGGGCGTGTTTTTTGTTCTGCGGGGGGCTCAACCTTTATTGACTGTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAcc                                                                                                                                    <  7:195003/151‑1 (MQ=2)
                 ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAACCGCTTCGGCGGGTTTTTGCTTTAG‑GAGGCGCAGAAAGATGAATGCCTGTCCACGAGACTATACCCAAAAGAAAGCGGCTTATCGGTAAGTTAAACATGTTTTACGTAATAAcc                                                                                                                                    >  7:352764/1‑151 (MQ=11)
                    ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGCGAGAAAGAAGAAACACTGTCGACGACACGATACGCAAAAGAAAGCGGCTTATAGCTCAGTTTAACCTGTTGTACGTAAAAACACg                                                                                                                                  >  6:527330/1‑150 (MQ=11)
                    ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTGCGGCGGGTTTTTGATTTTG‑GAGTGGCAGAAATAAAAAAGACTGTCCACGCCACTATACACAAAAGAAAACGGCTAATCTGTCAGTTTCACCTGATGTACGTAAAAACCCgg                                                                                                                                 >  6:380540/1‑150 (MQ=11)
                     tATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTT‑GAGTGGGATAAATAAAAAAGACATTCGACGAGAATATAAACAAAAGAAAGCGACATATCGGAAAGTTTAAAATTTTTTACGTAAAAAccaggt                                                                                                                                >  2:1011849/1‑147 (MQ=2)
                       tCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGTTGTACGTAAAAACCCggtcc                                                                                                                              >  5:1053034/1‑147 (MQ=255)
                       gctgTCAGCTTCCCCTGTTTTCCGACAACCCCCACTGCGGCGGGTTTTTGTGTTTG‑AGGGTGGTCAATCATGATTGCCTGGCCAAGACACTATCCACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTc                                                                                                                              <  3:481505/148‑1 (MQ=2)
                        cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGCTAcg                                                                                                                             >  7:744739/1‑151 (MQ=255)
                        cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGATTcg                                                                                                                             >  2:73038/1‑151 (MQ=255)
                          gTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTGcggc                                                                                                                           >  5:315360/1‑151 (MQ=25)
                           tCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTAcggcg                                                                                                                          >  1:526466/1‑151 (MQ=25)
                           tCAGTTTCACCTGGTTTACGTAAAAACCCGATTCGGCGGGTTTTTGATTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCTGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGCTTcggcg                                                                                                                          >  8:81403/1‑151 (MQ=11)
                                ttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGttt                                                                                                                     >  7:408284/1‑151 (MQ=35)
                                ttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGGGttt                                                                                                                     >  6:209468/1‑151 (MQ=255)
                                  cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGttttt                                                                                                                   >  3:650581/1‑151 (MQ=25)
                                  cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGGGtttat                                                                                                                   >  2:793615/1‑149 (MQ=255)
                                    ccTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGtttgtg                                                                                                                  >  5:449042/1‑147 (MQ=25)
                                    ccTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCCGTCGGTTTCACCTGATTTACGTAATAACCCGCTTCCGCGGGTTTGTAc                                                                                                                 >  7:1010266/1‑151 (MQ=11)
                                     cTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGGTACGGCGGGttta                                                                                                                    >  4:568538/1‑146 (MQ=255)
                                      tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTGCGGCGGGTTTTTGCtt                                                                                                               >  1:888000/1‑151 (MQ=255)
                                        tttcccttAAACACGCTCTTCGGATGTTTTTTGCTTTTG‑GAGGGGCATAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACttt                                                                                                              <  4:779665/143‑1 (MQ=11)
                                         tttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCGCTGCGGCGGGTTTGTGCttgtt                                                                                                            >  3:150910/1‑148 (MQ=11)
                                              gTAAAAACCCGCTTCGCCGGGTTTTTGCTATTG‑GAGGGGAAGAAAGATCAAACACTGTCAACGACACTATACCCAAAAGAAAGCTGCTAATCGTTCAGTAAAAACTCTTATACGTAAAAAACCGATAAGGCGCGtttaaaattatatag                                                                                                         >  6:1044864/1‑137 (MQ=2)
                                                 aaaaCCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATTCGGCGGGTTTATAATTTTTGAgtgcg                                                                                                     >  2:505233/1‑146 (MQ=11)
                                                   acccccCTCCGGCGGTTTTTTGCTTTTG‑CACGGCCCCAAACATCAATCACCCCCCACGACACTATACCCAAAAGAAAGCCCCTTATCGCTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGa                                                                                                  <  8:407741/149‑1 (MQ=11)
                                                    ccccccggcggcggGTTTTTGCTTTTT‑TAGGGCCAGAACGATCACGGACTCCCCACGTCTCGCTACCGACTCGACAGCCGACGATCGCTCAGCGTCTCCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGaa                                                                                                 <  3:150633/143‑1 (MQ=2)
                                                     cccGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGGTGCGGCGGGTTTGTCCTTGTTGAgtgcgagaa                                                                                                 >  5:219833/1‑142 (MQ=11)
                                                      ccGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTCATTTTGGAGTGGCAGaaat                                                                                               >  7:559002/1‑150 (MQ=16)
                                                       cGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTAATTTTGGAgggcccgat                                                                                                 >  1:449868/1‑142 (MQ=255)
                                                        gCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGGCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTTAAAATTTTGGAGTGGCAGaaata                                                                                              >  1:970362/1‑148 (MQ=11)
                                                         cggcgccggGTTTTTGGTTTTG‑TAGGGCCCCAAAGACCACTCACCCCCCACGACTCTCTACCCAAACGACATCGGCGTATCGGTCAGCTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGatg                                                                                            <  7:287154/145‑1 (MQ=2)
                                                          ttCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGGGTTTATAATTTTTGAGTGGCattaataaa                                                                                            >  2:585465/1‑142 (MQ=11)
                                                           tCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTCCTTTTTGAGGGGCAGAAAGttga                                                                                           >  7:273567/1‑150 (MQ=11)
                                                              gcggGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTGCGGCGGGTTTTAACTTTTGGAGTGGCAGAAAgaagaagc                                                                                        >  8:504399/1‑148 (MQ=11)
                                                                gggTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAACCCGGTTCGGCGGGTTTGATATTTTTGAGTGGCAgttagacatgagact                                                                                     >  7:365065/1‑137 (MQ=11)
                                                                        gCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTATAATTTTTGAGTGGCAGATATATGTATCGCTGTcggcg                                                                              >  8:347129/1‑146 (MQ=11)
                                                                             tG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTACTGCGGGTTTATACTTTTGGAGGCGGAGAAAGAGGTATGACTGGCCACGACACtg                                                                        >  4:1022916/1‑150 (MQ=11)
                                                                                gAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGGTACGGCGGGTTTATAATTTTGGAGGGGCAGATAGAAAAAACCCTTTCCACGAGACtat                                                                       >  6:601210/1‑150 (MQ=11)
                                                                                 aGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTACGGCGGGTTTTTCCTTGTGGATGGGGAGAAAGAGGAAGGAGAGTCCACGacacgacac                                                                     >  3:1038754/1‑146 (MQ=11)
                                                                                   gggCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCATAAAGAGGAATCACTGTCCACGAGACTATAcg                                                                    >  7:609079/1‑149 (MQ=255)
                                                                                   gggCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGGGTTTTTACTTTTTGAGTGCGAGATATTAGATAGACTGTCCACGACACGATAca                                                                    >  8:322659/1‑149 (MQ=11)
                                                                                     gCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGGTACGGCGGGTTTAAAATTTTGGAGTGCGAGATAGAATAATCACAGTCCGCGagacagaccgca                                                                  >  4:3329/1‑142 (MQ=0)
                                                                                      cAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCGCGACACTATACCCaa                                                                 >  7:419107/1‑150 (MQ=25)
                                                                                       aGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGATGAGTCCCTGTCGACGACACTATAcgctaa                                                                >  1:1075706/1‑145 (MQ=11)
                                                                                           aGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTGTACTTTTGGAGTGGGAGAAATATGAATCACTGTCCGCGACACTAAACTAAaaagaa                                                            >  6:296645/1‑150 (MQ=11)
                                                                                                aatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGATACGGCGGCTTTTAACTTTTGGACTGCCAGAAAGATTAATGAATGTCCACGAAACTAAACCCAaaacaaacacg                                                       >  8:236167/1‑146 (MQ=11)
                                                                                                    aCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGTAAGATGATAGTCAGTCGCCGACACTATAGCCAAATGAAAGccccttc                                                   >  1:773283/1‑144 (MQ=11)
                                                                                                    aCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGGGTTTGTAATTTTTGAGTGCCAGATATAAAAAACACATTCCACGACACGAGAAAAAaaagaaaacaacata                                                   >  8:693188/1‑142 (MQ=2)
                                                                                                        tCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGGTGCGGCGGGTTTATACTTTTTGAGTGCCAGAAATAAAAAACACATTCGACGACACTATAATCAAAAGAAAGCAGCTTATCgc                                               >  8:71138/1‑149 (MQ=11)
                                                                                                              gcacTATACCCAACATAAAGCGGCTTATCGCTCAGTTTCCCCTGTTTTACGTAAAACACGGGTGGTGCGTGTTTTTTGTTTTGGATGTGCTCAAATTTGAATGACTGGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                        <  7:687262/150‑1 (MQ=2)
                                                                                                              acacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGACGACTGACTTTCCACGACACTATACCAAAAAGAAAGCCGCTTATCGGTCAGttt                                        >  5:146967/1‑151 (MQ=11)
                                                                                                               cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGGAGAAAGAGGAATGACTGTCGCCGTCACTAGAGGCTAAAGCCAGCGGCTGATCGGTAAGtgtt                                       >  5:3562/1‑151 (MQ=11)
                                                                                                                acTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGCCAGATATAGGAAACACATTCCGCGAGACAAGAGAAAAAAGAAAGCAGATGATCGCTCAGtgat                                       >  5:356850/1‑147 (MQ=2)
                                                                                                                 cTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTCCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAACAAAAAGAAAGCGGCTTATCGGTCAGTTTTa                                      >  5:340711/1‑150 (MQ=16)
                                                                                                                 cTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCGACGTCACTATCCGCAAATGAAAGCGGCTTATCGGTCAGTTTTa                                      >  7:545437/1‑150 (MQ=255)
                                                                                                                  tttttaaaaaaCAAACCGGCGTAACGGCCAGATTCTCCTGTTTTACTTTATACCCCCCTTGTGCTGTTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAc                                     <  5:121308/143‑1 (MQ=2)
                                                                                                                  tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCGACGACACTATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTTAcc                                    >  1:165615/1‑151 (MQ=25)
                                                                                                                   ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCt                                   >  1:753957/1‑151 (MQ=35)
                                                                                                                    taCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAcgtt                                  >  1:666765/1‑148 (MQ=25)
                                                                                                                     aCCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTGTACTTTTGGAGTGGCAGAAAGATGAAGGCCTTGCGACGACACTATAGACAAAAGAAACCGTCTGATCGCTCAGTtataaattt                                 >  5:62181/1‑146 (MQ=11)
                                                                                                                     aCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTTTCCTTTTTGAGTGGCAGAAAGATGAATGACTTTCCCCGACACTCTACGCAAAAGAAAGCGGCTTATCGGTCAGTTTTaaatgt                                 >  3:779174/1‑146 (MQ=11)
                                                                                                                        gaagtgactggagattATGGGTGAGTTTTACCTTTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTTCTTTTGGAGGGGAATAAAGATTAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGt                              <  3:607752/137‑1 (MQ=11)
                                                                                                                         aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGATAGATGAATGACTGTCCACGACACTATCCACAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGt                              >  3:930235/1‑150 (MQ=255)
                                                                                                                         aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGt                              >  8:835191/1‑150 (MQ=39)
                                                                                                                            agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTTAACTTTTGGAGGGCCATAAAGAAGAAACACAGTCCCCGACACAAAACCCAAAAGAAACCGACTTATCGGTAAGTTATAAATGATGAACg                           >  6:1086175/1‑150 (MQ=11)
                                                                                                                            agaaagCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTTAACAGATGTACGt                          >  6:757192/1‑151 (MQ=255)
                                                                                                                             gaaagCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTTGAGGGGCAGAAAGATGAATGACTTTCCACGACAATATACCCAAAAGAAAGCGGCTTATCGGTAAGTTTTACCTGATGTAAGt                          >  6:23413/1‑150 (MQ=14)
                                                                                                                               aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGATAGATGACTGACTGTCCACGAGACTATACCCAAAAGAAAGCGGCTTATCGCTCAGTTTTACCTGATGTACGtaat                       >  1:205839/1‑151 (MQ=255)
                                                                                                                                   ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGAGGTACGtaatactc                   >  4:881911/1‑148 (MQ=255)
                                                                                                                                   ggCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTATGATTTTGGAGATCCAGAAAGATTAATGAATGTAAACGACACTATACCCAATAGAAAGCTGCTTATCGGTCAGTTTAAAAAGAATTACGtaataacc                   >  4:492615/1‑149 (MQ=255)
                                                                                                                                     gtgttcggtcggTTGCACCTGGTTTCGGTAAAAACCCGCTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                 <  7:880752/147‑1 (MQ=14)
                                                                                                                                     cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                 >  8:779248/1‑151 (MQ=255)
                                                                                                                                     cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATCAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                 >  5:612733/1‑151 (MQ=255)
                                                                                                                                     cTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGCCGGGTTTTTCCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATGTACGTAATAACCCg                 >  7:23589/1‑151 (MQ=255)
                                                                                                                                      ttATGGTTCAGTTTCACCTGGTTTACGTAAAAACCCTTTTCGTCGGGTTTTTGCTTTTGGTGTGGCCTAAAGATGATTGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACg                           <  6:338041/140‑1 (MQ=11)
                                                                                                                                      ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt                >  7:712384/1‑151 (MQ=255)
                                                                                                                                      ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACgagatagccagc                >  5:338542/1‑140 (MQ=11)
                                                                                                                                      ttATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGATTTTTGAGGGGCAGAAAGATGAATGACTGTCGACGACACTATACCAAAAAGAAATCGGATTATCGGTCAGTTTAACCTCATTTACTTAATAAAACGt                >  3:65836/1‑151 (MQ=11)
                                                                                                                                      ttATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGATTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGATTATCGGTCAGTTTTACCTTATTTACGTAATAAACCgg                >  6:1246/1‑150 (MQ=11)
                                                                                                                                       tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt               >  8:409467/1‑151 (MQ=255)
                                                                                                                                       tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt               >  6:235783/1‑151 (MQ=255)
                                                                                                                                        atcccgctctttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGTTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTc              <  1:1019707/142‑1 (MQ=255)
                                                                                                                                         tCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc             >  3:823068/1‑151 (MQ=255)
                                                                                                                                         cccgcccttttCACCTGATTTACGTAAAAACCCGCTGCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGATGAATTACTGTCCACTACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc             <  6:1005854/143‑1 (MQ=14)
                                                                                                                                          cgggccgTTTCACCTGTTTTACGTAAAAACTTTTTTTTTCTGTTTTTTAGTTTTGGTGGTTCATAAATTTCAATGACTGTCCTAGACACTATACCCATAATAACTGGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg            <  8:23524/145‑1 (MQ=255)
                                                                                                                                          cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAGCGTTCcg            >  2:17138/1‑151 (MQ=255)
                                                                                                                                          cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg            >  5:126921/1‑151 (MQ=255)
                                                                                                                                           ggTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg           >  7:883759/1‑151 (MQ=255)
                                                                                                                                           ggTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg           >  5:485511/1‑151 (MQ=255)
                                                                                                                                              cAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg        >  6:239219/1‑151 (MQ=255)
                                                                                                                                                gTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGt      >  1:262513/1‑151 (MQ=255)
                                                                                                                                                  ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt    >  5:495519/1‑151 (MQ=255)
                                                                                                                                                  ttCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtta              >  2:1019338/1‑140 (MQ=255)
                                                                                                                                                   tCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc   >  7:585078/1‑151 (MQ=255)
                                                                                                                                                    cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc   >  8:501612/1‑150 (MQ=255)
                                                                                                                                                    cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc   >  5:1068245/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTG‑GAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCA  >  DH1/613623‑613920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑