Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,519,3070CT24.0% 533.9 / 147.9 276G254G (GGG→GGA) yfaDtransposase
Reads supporting (aligned to +/- strand):  ref base C (112/91);  new base T (46/18);  total (161/115)
Fisher's exact test for biased strand distribution p-value = 1.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

TAATCTCTCCTTTTAATGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACAGTGTTTGTAGCTGGCTGTCATT  >  DH1/1519157‑1519456
                                                                                                                                                                              |                                                                                                                                                     
tAATCTCTCCTTTTAATGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTTTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGc                                                                                                                                                       >  6:5331/1‑151 (MQ=255)
     tctcCTTTTAATGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTc                                                                                                                                                  >  7:298970/1‑151 (MQ=255)
       tcCTTTTAATGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCt                                                                                                                                                >  4:925344/1‑151 (MQ=255)
           tttAATGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCg                                                                                                                                            >  8:603400/1‑151 (MQ=255)
            ttAATGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGt                                                                                                                                           >  8:480499/1‑151 (MQ=255)
             tAATGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGt                                                                                                                                           >  4:226578/1‑150 (MQ=255)
             tAATGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGt                                                                                                                                           >  3:61399/1‑150 (MQ=255)
              aaTGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGt                                                                                                                                           >  1:391586/1‑149 (MQ=255)
                tGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGcc                                                                                                                                       >  6:107846/1‑151 (MQ=255)
                     acgtggtctatgcggaTCTGTTAGGTGGGTGGGCGCGAGCACATTGTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCa                                                                                                                                  <  3:1066633/136‑1 (MQ=18)
                        cgcaaTATCAGCCTCAGTGAGTTGGGTTCTCGCCAGCACAATCTCACGTTTTTATTTTTTTTCCAGGTTGCCCAAAGATATTTAAATTGGTTTTTAATGT‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TTTCTTTCTTCGAGGCTATTTGCTTCTCTTCCTGCCGTAGCCGTTCAGCa                                                                                                                               <  2:100178/148‑1 (MQ=255)
                        cGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGc                                                                                                                                >  6:121270/1‑150 (MQ=255)
                         ggAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCa                                                                                                                               >  5:235870/1‑150 (MQ=255)
                             tatCAGCATCAGTGAGTTGGGTTGTCGCCCGCACAATCTCACGTTCAAAGCCCTGCTCCCGCATGCGCAAAGCAATTTTAATGGCTTGTTCCTGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAAGAGt                                                                                                                          >  6:1052580/1‑151 (MQ=255)
                             tatCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGt                                                                                                                          >  2:660324/1‑151 (MQ=255)
                              atCGGCTTCGGTTAGGTGGGTGGCCGCGAGCACATTGTCACGATCAAAGCCCTGATCCAGAATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTc                                                                                                                         <  2:145542/151‑1 (MQ=14)
                                        gTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAAtctct                                                                                                               >  8:521169/1‑151 (MQ=255)
                                         ggcggtgggggggcGCCAGCTCAATCTCACGTTCAACGCCCTGCACCAGCATGCTCAAAGCAATTTTAATGCCTTGTTCATCC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAAtctctc                                                                                                              <  7:807359/138‑1 (MQ=25)
                                            gTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCtt                                                                                                           <  2:391213/151‑1 (MQ=255)
                                                 gTTGTCTCCCGATCAATGTCACGTTCAAAGCCCTGCTCCACCATGCGCAAAGCAATTTTAATGTCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTa                                                                                                      <  5:759672/151‑1 (MQ=21)
                                                 gTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGt                                                                                                       >  1:244627/1‑150 (MQ=255)
                                                  gggtctcaaGCACAATCTCCCGTTCAACGCCCTGGTCCAGCATTCGCAAAGCAAGTTTAATCGCTTGTTCATCC‑‑‑‑‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAg                                                                                                     <  5:411981/143‑1 (MQ=21)
                                                  ggggcGCCAGCACAATGTCACGTTCAAAGCCCTGCTCCGGAATGCTCAAAGCAATTTTAATCGCATGTTCATCC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAg                                                                                                     <  1:432062/147‑1 (MQ=25)
                                                   tGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAgt                                                                                                    >  8:805961/1‑151 (MQ=255)
                                                     tCGCCACCACCAGCCCACGCTCAACCCCCCGCTCCCCCATTCGCAACGCAAGTTAATTCGCTGCTTCTACC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGGCGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAgt                                                                                                    <  8:1044370/149‑1 (MQ=9)
                                                        caaGCACAATGTCACGTTCAAAGCCCTGCTCCAGCATGCTCAAAGCAATTTTAATGCATTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGa                                                                                               <  3:510622/149‑1 (MQ=255)
                                                         cAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGa                                                                                               >  4:866482/1‑150 (MQ=255)
                                                            cacaATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGt                                                                                           <  6:877526/151‑1 (MQ=255)
                                                             acaATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGtt                                                                                          >  3:895172/1‑151 (MQ=255)
                                                              caaggtcACGATCAAAGACCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTCGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTc                                                                                         <  5:445550/146‑1 (MQ=255)
                                                                aTCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCgg                                                                                       >  2:350794/1‑151 (MQ=255)
                                                                           aaaGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATctcctc                                                                             >  1:759620/1‑150 (MQ=255)
                                                                            aaGCCCTGCACCAGCATGCGCAAAGCAATTTTAATCGCTAGTTCAACC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGGAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCaa                                                                           <  7:1059073/151‑1 (MQ=25)
                                                                               cccTGTTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGCAAACCTTCTAATA‑‑‑‑‑‑‑‑‑‑‑‑TACCTTACTGACAGCCAATTTGATGTCCTTCCTGCCGAAGCCGTACAGCAATAGTCATTAATATCTCATTTTTTtgtgattaaggatcataa                                                                                     >  8:139731/1‑136 (MQ=21)
                                                                                ccTGTTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑AAACCTTCTAATTTACCTTCCTGCCCGCCAATTTGATGTCCTTCCTGACGAAGACGTTCAGCAATAGTCATTAATCTCTCCTTTTGTTGTCGTGacgcatcgacatc                                                                                   >  1:414334/1‑139 (MQ=21)
                                                                                 cTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATaaa                                                                       >  4:810452/1‑150 (MQ=255)
                                                                                  tGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAAc                                                                     >  2:825873/1‑151 (MQ=255)
                                                                                    ttCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑AAACCTTCTAATTTACCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCATCAATatcc                                                                               >  5:1028281/2‑151 (MQ=18)
                                                                                    cTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACgg                                                                   >  2:51229/1‑151 (MQ=255)
                                                                                    cTCCAGAAAGCCCAAAGCAATTTAAATTGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCACACAATTAGATGAAATTCCTCCCGTAGCCCATCAGAAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTACGACAAACTCCTCAATAAAACgg                                                                   >  4:780064/1‑151 (MQ=25)
                                                                                      ccAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCACCCAATTTGATGCCCTTCCTGCCGTAGCCCTTCAGGAATAGTCATTAATCTCTCCTTGTCTACTGGTGAACGTTCGGCAATATCCTCAATAAAACGGGt                                                                 >  7:255425/1‑151 (MQ=37)
                                                                                       cAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTg                                                                >  6:316469/1‑151 (MQ=255)
                                                                                        aGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGa                                                               <  6:57447/151‑1 (MQ=255)
                                                                                         gCATGCTCAAAGCAATTTTAATGGCATGTGCATCC‑‑‑‑‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCCTGACTGGACTTCAGATGTGCTCGCTTCCGTTCGCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGaa                                                              <  3:854674/151‑1 (MQ=255)
                                                                                          cATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTCaa                                                              >  3:266835/1‑150 (MQ=255)
                                                                                           attataaaaGCATTTTTAATGTCTTGTTCAAGC‑‑‑‑‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑‑‑‑‑CACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAAc                                                            <  1:969703/145‑1 (MQ=25)
                                                                                             gcgcAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCATCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACg                                                           <  1:1065315/150‑1 (MQ=255)
                                                                                               gcAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACggg                                                         >  2:722331/1‑150 (MQ=255)
                                                                                                 aaaGCAATTTTTATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAgg                                                      >  5:142565/1‑151 (MQ=255)
                                                                                                       aTTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATcgc                                                >  6:252211/1‑151 (MQ=255)
                                                                                                         tttAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATcgccg                                              >  8:598862/1‑151 (MQ=255)
                                                                                                          ttAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATcgccgc                                             >  4:580508/1‑151 (MQ=255)
                                                                                                           tAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATcgccgc                                             >  1:673642/1‑150 (MQ=255)
                                                                                                                gcgtGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCGAGCCACTTTGAGCCCCTTCCTGCCTTAGGAGCTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTgca                                       <  3:678796/148‑1 (MQ=38)
                                                                                                                  ttGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTgcagc                                     >  5:511985/1‑151 (MQ=255)
                                                                                                                   tGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTgcagc                                     >  3:979647/1‑150 (MQ=255)
                                                                                                                    gTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTgcagca                                    <  2:98518/150‑1 (MQ=255)
                                                                                                                    gTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTgcagca                                    <  5:924673/150‑1 (MQ=255)
                                                                                                                    gTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTgcagc                                     >  7:170079/1‑149 (MQ=255)
                                                                                                                    gTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTAACTCCGCATTgcagcat                                   >  5:299253/1‑150 (MQ=255)
                                                                                                                        aTGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTACTGCCGTAGCAGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACCGGTGAAACCGGAGGTATAGCCGCATTGCAGCAGATa                                >  3:563559/1‑150 (MQ=255)
                                                                                                                         tGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAAt                              >  7:21007/1‑151 (MQ=255)
                                                                                                                          tgactccttctatta‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCAtt                                          <  7:13177/136‑1 (MQ=255)
                                                                                                                                         aaccttctaatttACCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTGGGCAAGCTCAGCAATAACACGGGTGAACCGGGAGGTATCGCCGCATAgcac                                      >  8:361415/13‑150 (MQ=17)
                                                                                                                                            gttcatatttACCTTCCTGCCATCCAATTTGATGTCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTgcagca                                    <  4:37540/141‑1 (MQ=255)
                                                                                                                                              ttcttcctatcTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCTCCGCATTGCAGCAGa                                  <  6:861531/140‑1 (MQ=25)
                                                                                                                                               ctaatttACCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTAAATAAAACGGGt                                                                 >  1:880345/7‑118 (MQ=17)
                                                                                                                                               ctaatttACCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATATCATCAATAAAACGTGTGAAACGGGAGGTATcgccg                                              >  1:794708/7‑137 (MQ=18)
                                                                                                                                               ctaatttACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATcgccg                                              <  2:794231/131‑1 (MQ=255)
                                                                                                                                               catatttACCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGAt                                 <  6:1027389/144‑1 (MQ=255)
                                                                                                                                                 attttACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAAt                              <  5:823929/147‑1 (MQ=255)
                                                                                                                                                 aatttACCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTTAAGAGGATAACGTTAGGCAAAATCCTAAATAAAACTTGTGAAACGGGAGCTATCGCCGAATTCCACCAGATAAt                              >  6:741774/5‑151 (MQ=14)
                                                                                                                                                  cccgacggtcctccGAGCTAAGTTGATGGCCTTCCTGCCGGAGCCGTGCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGGATCGCCGCATTGCAGCAGATAAtt                             <  2:950503/143‑1 (MQ=37)
                                                                                                                                                  atttACCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGAGGTGAACGTTGGGCAAACTCATCAATAACACGGGTGAAACGGGAGGTATCGCCTCATTGCAGCAGATAAtt                             >  7:912533/4‑151 (MQ=21)
                                                                                                                                                   tttACCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGAAGCAGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGAATTGCAGCAGATAAtt                             >  6:688311/3‑150 (MQ=255)
                                                                                                                                                   atttccTTCCTGCCATCCAATTTGATGTCCTTCCTGCCGTAGCATTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTa                            <  6:613457/147‑1 (MQ=255)
                                                                                                                                                    ttACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAgtggtg                                                                                                <  1:130506/81‑1 (MQ=255)
                                                                                                                                                     tACCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGAAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTTACCGTTCGTCAGTCTCCTCAATAAAACGGGTGAAACGCGCGGTATcgcc                                               >  5:368112/1‑130 (MQ=18)
                                                                                                                                                     tACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTaa                           >  3:192388/1‑150 (MQ=255)
                                                                                                                                                     tACCTTCCTGCCAGCCAATTTGATGACCTTCCTGCCGTa                                                                                                                                          >  3:143441/1‑39 (MQ=255)
                                                                                                                                                      aCCTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGGAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTAAAGTGGTGAACGTTCGGCAAGATCATCAATAAAAGGTGTGAAACGGTAGATATCGCAGAATTGCAGCAGATAATTaat                          >  7:698821/1‑149 (MQ=18)
                                                                                                                                                      aCCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAAc                         <  8:756717/151‑1 (MQ=255)
                                                                                                                                                       ggtgctctgccgctcaTTTGATGTCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACa                        <  5:1081602/136‑1 (MQ=38)
                                                                                                                                                       ccTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGAAGCCGTTCAGCAATAGTCATTAATCTCTCCTTATGAAGTGCTGAACGTTATGCAAACTCCTGAATAAAACGGGTGAAAAGCGAGGTATCGCCGAAATACAGCAGATAATTAAAc                         >  8:872199/1‑150 (MQ=18)
                                                                                                                                                       ccTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACa                        <  8:712209/151‑1 (MQ=255)
                                                                                                                                                        cTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAAt                                                                          >  5:864949/1‑100 (MQ=255)
                                                                                                                                                        cTTCCTGCCAGCCAATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTATAGTGATGAACGAACGTCAATATCCTCAATTAAACTGCTCAACCGCCAGGTATCCCCGAAAACCAGCAGATAAtacatccg                       >  7:472435/1‑144 (MQ=9)
                                                                                                                                                          tCCTGCCAGCCAATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCAt                                           >  5:684629/1‑129 (MQ=255)
                                                                                                                                                          tCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACgg                                                          <  7:751812/114‑1 (MQ=255)
                                                                                                                                                              gccagccaATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACAGTGtt                   <  8:913034/149‑1 (MQ=255)
                                                                                                                                                              gccagccaATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACAGTGTTTg                 <  7:535465/151‑1 (MQ=255)
                                                                                                                                                              gccagccaATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACAGTGTTTg                 <  2:523941/151‑1 (MQ=255)
                                                                                                                                                              gccagccaATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACAGTGTTTg                 <  1:348454/151‑1 (MQ=255)
                                                                                                                                                              gccagccaATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCATCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACAGTGTTTg                 >  8:650835/1‑151 (MQ=255)
                                                                                                                                                                cagccaATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATa                                                                         >  1:51755/1‑93 (MQ=255)
                                                                                                                                                                cagccaATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTAGGCa                                                                                     >  6:39766/1‑81 (MQ=255)
                                                                                                                                                                 agccaATTTGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGGGAACGATCGGCAAtctc                                                                                >  1:590499/1‑85 (MQ=17)
                                                                                                                                                                 agccaATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACAGTGTTTGTAg              <  2:7073/151‑1 (MQ=255)
                                                                                                                                                                        ttGATGTCCTTCCTGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACggg                                                         >  3:644770/1‑101 (MQ=255)
                                                                                                                                                                         tgctGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACAGTGTTTGTAgctggctg       <  3:455028/147‑1 (MQ=255)
                                                                                                                                                                              |                                                                                                                                                     
TAATCTCTCCTTTTAATGACAGTTCGGAATATCAGCATCAGTGAGTTGGGTTGTCGCCAGCACAATCTCACGTTCAAAGCCCTGCTCCAGCATGCGCAAAGCAATTTTAATGGCTTGTTCATGC‑‑‑‑‑‑‑‑‑‑‑‑A‑‑‑‑‑‑‑‑‑‑‑‑TACCTTCCTGCCAGCCAATTTGATGCCCTTCCTGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTTTGTAGTGGTGAACGTTCGGCAATCTCCTCAATAAAACGGGTGAAACGGGAGGTATCGCCGCATTGCAGCAGATAATTAAACAGTGTTTGTAGCTGGCTGTCATT  >  DH1/1519157‑1519456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑