Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,169,5770TG24.8% 166.8 / 29.5 151R661R (CGA→CGC) rsxCelectron transporter RsxC
Reads supporting (aligned to +/- strand):  ref base T (30/76);  new base G (3/32);  total (42/109)
Fisher's exact test for biased strand distribution p-value = 2.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

CTGTTCTTCTGGTACCGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTG  >  DH1/2169427‑2169725
                                                                                                                                                        |                                                                                                                                                       
atGTTCTTCTGGTACCGCATTAGCCTGTTGCTGTTCGAGCTTTCGTGCTTTGGCGCGTGCAATAGCCGCTTCTACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCt                                                                                                                                                         <  6:219933/150‑1 (MQ=255)
  gTTCTTCTGGTACCGCATTAGCCTGTTGCTGTTCGAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCt                                                                                                                                                         <  6:455097/149‑1 (MQ=255)
  gTTCTTCTGGTACCGCATTAGCCTGTTGCTGGTCCAGCTTGCGTGCTTTGGCGCGGGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCTACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTc                                                                                                                                                        <  8:645459/150‑1 (MQ=255)
    tcttctGGTACCGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCgg                                                                                                                                                      <  3:699145/150‑1 (MQ=255)
       tctGGGACGTCATTATCCTGTTGCTGTTCCTGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGGTCCGGATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCa                                                                                                                                                   <  1:544264/150‑1 (MQ=37)
       tctGGGACCGCATTAGCCTGCTGCTGTGCGAGCTTGCGGGCTTTGGCGCGTGCAATAGCCGCTGCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCggg                                                                                                                                                     <  2:24860/148‑1 (MQ=25)
          tgttcggctttAGCCTGTTGCTGTTCGAGCTTGCTTGCTTTTGCGCGTGCAATAGCCGCTGCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATa                                                                                                                                                <  2:385107/141‑1 (MQ=25)
          ggTACCGCATTAGCCTGTTGCTGTTCCCGCTTGCGGGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCaa                                                                                                                                                  <  4:405417/148‑1 (MQ=255)
          ggTACCGCATTACCCTGCTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATa                                                                                                                                                <  4:255162/150‑1 (MQ=255)
           gTACCGCATTAGCCTGGTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGc                                                                                                                                              <  4:472407/151‑1 (MQ=255)
             aCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCACCGGGGAATTCGGCCACGGCAAAATCCAGTTGC‑TCTTCACCATTGCGGGCGTGGGCGCGGGCATTAGCgg                                                                                                                                            >  5:421512/1‑151 (MQ=9)
               cGCATTAGCCTGGTGCTGTTCCAGCTTGCGGGCTTTGGCGCGGGCAATAGCCGCTTCGACTGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCt                                                                                                                                          <  1:570235/151‑1 (MQ=255)
               cGCATGAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCt                                                                                                                                          <  5:785262/151‑1 (MQ=255)
                 ttttATCCTGTTGCTGTGCGTGGTTGTTGGCTTTTGCGCGTGCAATAGCCGCTTCGACTGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCtt                                                                                                                                         <  2:645425/148‑1 (MQ=18)
                  aTTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCg                                                                                                                                       <  5:633470/151‑1 (MQ=255)
                  aTTAGCCTGGTGCTGTTCCAGCTTGCGGGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCg                                                                                                                                       <  5:475112/151‑1 (MQ=255)
                   atAGCCTGGTGCTGGGCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCGTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGa                                                                                                                                      <  5:290193/150‑1 (MQ=255)
                     aGCCTGTTGCTGTGCGAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGAcg                                                                                                                                    <  3:1061670/151‑1 (MQ=255)
                      gCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCGGCTTTGCAGCGG‑TAGAGCACAGGTGGTGGCTCCGGTGTATCCGGAGGA‑TGTTCCCGGTTGCGCGCTTTGGCTCGTGCAATAGCGGCTTCgcggg                                                                                                                                   >  1:694515/1‑147 (MQ=9)
                      gCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGAcgg                                                                                                                                   <  1:844017/151‑1 (MQ=255)
                      gCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGATCCGCATTACCCTATTGC‑TGATACAGGTTGCACCCTATGGAACGGGCAATAGCGGAATCGAcgc                                                                                                                                   >  6:303216/1‑150 (MQ=11)
                          gctGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTGCTACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGAcggcgg                                                                                                                                <  2:450833/148‑1 (MQ=255)
                          gTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCTACCTGTTCTTCTGGTTCCGCATTATCGGGTTCG‑AGGACCACTGAGCTCGCGTAGGGACGGGGAATAGCGGCTTCGAcggcggg                                                                                                                               >  7:315027/1‑150 (MQ=2)
                          gTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCGACCTGTTCTTCTGGATCCGCATTATCCGGATGC‑GGGACCAGTTAGCTCGATTTCCGCCGGGCAATAGCGGCTTCGAcggcggg                                                                                                                               >  7:319750/1‑150 (MQ=14)
                           gtGCTGTTCCAGCTTGCGTGCTTGGGCGCGTGCAATAGCCGCTGCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCt                                                                                                                              <  7:981374/150‑1 (MQ=255)
                            tGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCGAGCTCTTCTGCTGGCTCCTCTTTACGCGGTTGC‑TGTTCCAGATTGCGCGCTTTGGCTCGGGCAATAGCGGCTTAGACGGCGGCtt                                                                                                                             >  3:135679/1‑151 (MQ=14)
                             gctggtcgcggttGCGGGCGTTCGCGCGGGCAATAGCCGCTGCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCttt                                                                                                                            <  5:375090/140‑1 (MQ=17)
                             gCTGTTCCCGCTTTGTTGTTTTGGCGCGTTCAATCGCTTCCTCTACGGGTGGCTTTT‑GCGTTGTAACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGC‑TGTTCCAGCTTGCGGGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCttt                                                                                                                            <  2:1059589/151‑1 (MQ=2)
                               tGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCGGCTTTGC‑GCGGATCGACCGGTGGTTATGGCTCCGCAGTGGACTGTTGG‑GGGTCCGGATTGCGGGCTTTGGCTCGGTGATTAGCGGCTTAGACGGCGGCTTTgg                                                                                                                          >  3:929590/1‑150 (MQ=2)
                                   cgacgttGCGTGCTTTGGCGCGTGCAATAGCCGCTTCTACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCgg                                                                                                                      <  8:275910/146‑1 (MQ=255)
                                   cgaGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCTACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGcgcg                                                                                                                       <  2:418459/148‑1 (MQ=255)
                                   cccccctGCGCGCTTTGGCCCGTGCAATATCTTATTCTACGGCTGCTTTGC‑GAGTATAAAACTGTTCTTCTGGCTCTGCATTAGCATGTTGC‑TGTTCCATTTTGCGCGCTTTGGCTCGTGCAATAGCGGCTTCGACGGCGGCTTTgcgc                                                                                                                        <  8:190007/143‑1 (MQ=2)
                                   ccAGCTTGCTGGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTGCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCgg                                                                                                                      <  3:41653/151‑1 (MQ=255)
                                    cAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCGTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGa                                                                                                                     <  8:533462/151‑1 (MQ=255)
                                     cgcttgcttgcttTTGCGCGTGCAATAGCCGCTTCTACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGAt                                                                                                                    <  8:800837/150‑1 (MQ=255)
                                     aGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCGGCTATCA‑CCGGATAGCCGAGTTATTCTGGATCCTCATTAGCCCGTTGG‑TGTTCACGGTTGAGCGCTTTGCCTAATAAAATAGCGGCTTCGACGGCGGCttggggggg                                                                                                                      >  8:294391/1‑142 (MQ=2)
                                      gCTTGCGTGCTTTGGCGCGTGCAATAGCCGCGTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATc                                                                                                                   <  7:929424/151‑1 (MQ=255)
                                       gttGCGTGCGTTGGCGCGTGCAATAGCCGCTGCTACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCg                                                                                                                  <  1:157728/150‑1 (MQ=255)
                                         tGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGCCGGCAGCTTTGC‑GCGGATCGACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCAGATTGCGCGCTTTGGCTCGGGCAATAGGCGCGGCGGCGGCGGCGTGGGGCggcgcgtc                                                                                                                >  5:278094/1‑145 (MQ=14)
                                          gcgcgctttttattttGCTATAGCGGCTTCTACGTCTGCTATTC‑TCGGTTCGACATGCTTTTCTTTCTCGCCTGTATCCTGTTGT‑TGTTCCAGCTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGAc                                                                                                                <  4:928942/135‑1 (MQ=2)
                                          gCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCCGCTTTGC‑GCGGATCGCACTGGTCTTCTGGGTCAGGCTTATCCGGTTGC‑TGTTGCGGGTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGGTTTGCGGGGATCgggg                                                                                                               >  7:190134/1‑148 (MQ=14)
                                           cGTGCGTTGGCGCGGGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTGCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGAcc                                                                                                               <  3:676467/150‑1 (MQ=255)
                                            gTGCGTTCGCGCGGGCAATAGCCGCTTCTACTGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGC‑TGTTCCAGTTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCtg                                                                                                             <  2:836992/151‑1 (MQ=14)
                                              gcgttGGCGCGGGCAATAGCCGCGTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCtgtt                                                                                                           <  1:310334/148‑1 (MQ=255)
                                              gCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAGCAAGTACTACAGGCTACGAATTAGACAGTTGC‑TGTTCCAGTTTGCGAGGTTTGGCTCGGGCAATAGAGGATTCAACGGCGGCTTTGCGCGGATCggcggggt                                                                                                           >  8:1010891/1‑144 (MQ=14)
                                              gCTTTGGCGCGTGCAATAGCCGCTGCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCtgtt                                                                                                           <  3:494626/151‑1 (MQ=255)
                                                 ttGGCGCGTGCAATAGCCGCTTCGACTGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCtgttg                                                                                                          <  8:577352/149‑1 (MQ=255)
                                                 ttGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCCACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGCTTGCGCGCAAGCGCTCCGGGAATACCTGCTTGGACGGCGGAGATGCGCGGATCGCCCtgttgtg                                                                                                        >  5:138853/1‑150 (MQ=255)
                                                    gcgcGGGCAATAGCCGCTTCTACTGCCGCTTTGC‑TCGTATCAACCTGTTCTTCTGGAACCGCATTATCCTGTTGA‑TGTTCCTGTTTGCTTGCTTTTGCTCGGGCAATAGCCGCTTCTACTGCGGCTTTGCGCGGATCGACCTGTTCTTCTg                                                                                                     <  2:849167/151‑1 (MQ=11)
                                                     cgcgTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCACCCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTgg                                                                                                    <  7:884252/151‑1 (MQ=255)
                                                     cgcgTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTgg                                                                                                    <  3:536303/151‑1 (MQ=255)
                                                        gTGCAATTGCAGCTTCTACTGCCGCTGTGC‑GCGGAGCAACCTGTTCTTCTGGCTCCTCATTAGCCGGGTGA‑TGGTCGCGTTTGCGGGCTTTTGCTCGGGCAATAGCGGCTTCTACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTc                                                                                                 <  6:357945/151‑1 (MQ=14)
                                                           cAATAGCGGCGTCGCCCGCAGCTTTGC‑GCTGCCGCTCCTGATCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑c                                                                                            <  6:1054834/151‑1 (MQ=14)
                                                           cAATAGCCGCTGCGACTGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑c                                                                                            <  3:604057/151‑1 (MQ=255)
                                                              tAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCCACCTGTTCTTCTGGATCCGCATTAGCCGGCTGC‑TGATCCCGCGTCCGCCCTGAGGTACGGCCAGTGCCGGGTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGGTCGG‑‑cggt                                                                                         >  7:769940/1‑148 (MQ=11)
                                                               aGCCTCTTCTACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCGCGGGCAATAGCGGCTTCTACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑tt                                                                                        <  8:539388/150‑1 (MQ=255)
                                                               aGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGGTGC‑TGTTCCCGTTTCCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTa                                                                                       <  3:89552/151‑1 (MQ=255)
                                                                gCCGCTTCGACGGCAGCTTTGC‑GCGGATCGACCTGTTCTTCTGGCT‑CGGAATACCCCGCTGCGTCAGGCCGGTAACTCCCTATGGCGCGGGCAATAGCGGCTTCGACGGAGGCTTTGCGCGGATCGCCCTGGGGGGCGGGCTCGG‑‑cggggg                                                                                       >  1:477753/1‑146 (MQ=2)
                                                                 ccGCTTCTACTGCAGCTTTGA‑GCGGATCAACCTCTTCTTCTGGCTCCGCATTAGCCTGTTGC‑TGTTCGCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGc                                                                                     <  4:690994/151‑1 (MQ=14)
                                                                       cGACGGCAGCTTTGC‑GCGGATCATCGGGTGCGTCTGGCTCGGGATGAGCGGGTGGC‑TGTGGCCGTTTGCGCGCTTGTGCGCGGGCAATAGCCGCTGCTACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTg                                                                               <  5:882027/151‑1 (MQ=17)
                                                                       cGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGC‑TGTTCCACGATGCGATGAGAGGCGCGCTGAAGAGCGGGGACTCCGGCGCCTGTGCGCGGCTCGACCTGTTGTTGTGGATCGG‑‑CA‑TTAGCCTGTTg                                                                               >  7:580184/1‑151 (MQ=2)
                                                                              ggatttGC‑GCTGTTCTACGTCGTCTTCTGGCTCCGCTTTAGCCTTGTGC‑TGTTCCGGTTTGCGGGCTTTGGCGCGGGCAATAGCGGCTTCGACTGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTcc                                                                        <  1:396822/148‑1 (MQ=11)
                                                                                    gc‑gcGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGC‑TGTTACATATTAAGAGCTTAGTCTAGGGAAATAGAGTCTTCGACGGAGTATATGAGCGGATAGACCTGTTGTTCTGTATAAA‑‑CA‑TTAGCCTGTTGCTGttagagcgtg                                                                  >  4:707662/1‑143 (MQ=9)
                                                                                          gagccacatgctcttctGGCTCGGCTTGAGCCGGGTGC‑TGGGCGGGTTTGCGGGCGTGTGCGCGGGCAATAGCGGCTGCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTg                                                              <  2:782916/139‑1 (MQ=14)
                                                                                          gagcAACCTGTGCGTCTGGCTCAGCATTCGCCTCTTGC‑TGTGCCCGTTTGCGCGCTTTGGCGCGGGCAATAGCGGCTGCTACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGc                                                             <  7:496923/148‑1 (MQ=14)
                                                                                            tCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGATTGCGCGCTTTGGCACGGGCAATACCGGCTTCGACGGCTGCGTAGCGCGGAGGGAACTGTAATACTGGGTACG‑‑CA‑TTAGCATGTTGCTGTTCGAGCTTgatgcatt                                                           >  8:347786/1‑144 (MQ=2)
                                                                                             cAACGTGGTCTTCTGCCTCGGCATAAGCCGGGTGC‑TGTGCGCGTTTGCGGGCTTTCGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCtt                                                           <  7:856332/150‑1 (MQ=17)
                                                                                              aaCCTGTTCTTCTGTCTCCTCATTAGCCGGTTCC‑TGTTCCCGCTTCCCCGCTTTGGCTCGGTCAATAGCGGCTTCTACTGCGGCTTTGCGCGGATCTACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTg                                                         <  6:124282/151‑1 (MQ=14)
                                                                                               aCCTGTTCTTCTTTGTCCTCATTTTCGGGGTGC‑TGTTCTGGTTTGTTGGCTTTGGCGCGGGCAATAGCGGCTGCTACTGCGGCTTTGCGCGGATCTACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTgg                                                        <  8:472542/151‑1 (MQ=14)
                                                                                               aCCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCt                                                                                                      >  6:623607/1‑108 (MQ=255)
                                                                                                ccaccagttcggccTCCGGATGAGCCTTGTGC‑TGGTCCGGGTTGCGCGCGTGCGCGCGGGCAATAGCCGCTGCGACGGCCGCTTTGCGCGGATCCACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGc                                                       <  1:147658/138‑1 (MQ=2)
                                                                                                ccTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGATCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGc                                                       >  3:359460/1‑151 (MQ=255)
                                                                                                 cccaccgccgcgCTCCGCACTCGCCGCTTGC‑TGTTCCCGTTTGCTTTCTTTCGCTCGGGCAATAGCCGCTTCGTCTGCGGCTTTGCTCGTTTCGAGCTGTTG‑TC‑GCTTCGGCTCT‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCa                                                      <  3:809067/140‑1 (MQ=9)
                                                                                                  agcttttctGGCTCGTGATCACCCTGCTGC‑TGTGCGCCTTTGTGGGCTTTCGCGCGGGCAATAGCCGCGTCTACTGCGGCTTTGCGCGGATCTACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCa                                                      <  6:922887/145‑1 (MQ=2)
                                                                                                   ggtcttctGGGTGCGCATTAGCCGGTTGC‑TGTTCCCGCTTGCGCGCTTCGGCGCGGTGAATAGAGGGCTCGACTGCGGGGTTGCGCGGATCCACCTGTTGTTCTGGGTCCG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACg                                                    <  1:316919/149‑1 (MQ=11)
                                                                                                     tcttctGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGAAGGCGGCTTTGCGCGGATCGACATCTTCATCGGGATCGG‑‑AA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACggg                                                  >  7:776021/1‑151 (MQ=14)
                                                                                                        tctGGCTCCTCATTAGCCGGTTGC‑TGGTCGCGTTTGCGCGCTTTGGCGCGGGCAATAGCGGCTTCGACTGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGc                                                 <  4:528620/149‑1 (MQ=255)
                                                                                                        tctGGCTCCGCATTAGCCTGGTGC‑TGGTCCGGTTTGCGCGCGTTGGCTCGGGCAATAGCGGCGTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTACCAGCTTGCGTGCTTTGGCACGGGc                                                 <  3:866242/149‑1 (MQ=14)
                                                                                                          tGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCTGCTTCGACGGCAGATTTACGAGGATCGCCAACTAGATCTGGATCGC‑‑CA‑GTAGCATGTTAATGTTCAATCTTCCGGGGTATGTCTCGGGcgaa                                              >  2:855845/1‑147 (MQ=255)
                                                                                                           ggCCCCGCATTAGCCGGTTGC‑TGCTCCCGCTTGCGCGATTTCGCGCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCTACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATa                                             <  7:220016/150‑1 (MQ=14)
                                                                                                             cTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGGTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGGGGTTTCGCACggggctgagcg                                          >  7:953102/1‑143 (MQ=14)
                                                                                                               ccGCATGAGCCTGTTGC‑TGTTCGCCTTTTCGCGCCTTCGCTCGGGCAATAGCGGCGTCTACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCg                                          <  5:107050/149‑1 (MQ=14)
                                                                                                                gtctttAGCCTGTTGC‑TGTTCGGGTTTTCTGGCTTTTGCGCGGGCAATAGCCGCTTCTACTGCGGCTTTGCGCGGATCTACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCt                                       <  2:560467/147‑1 (MQ=14)
                                                                                                                   aTTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGACTACG‑‑GA‑TTAGCCTCTTGCTGTTCAAGCTTGAGTTCTATGGGCAGGGCAATCGCggatgcg                                    >  4:156670/1‑146 (MQ=14)
                                                                                                                      agccttttGC‑TGTTCGCGTTTGCGCGCGTTGGCTCGGGCAATAGCCGCTTCTACGGCCGCTTTGCGCGGATCTACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAcg                                 <  5:399803/145‑1 (MQ=14)
                                                                                                                      aGCCTGGTGC‑TGGTCCCGTTTGGTCGCTTTGGCGCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCCACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAcg                                 <  7:1017105/151‑1 (MQ=14)
                                                                                                                      aGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCTGCTTCGACGGCAGATTTACGAGGATCAGCAACTTGTTCTAGATCGG‑‑AA‑TAACCAAGTTGATGTTAAAACTTTCGTGCTGTGGCTAGCTCTATATCGGCTTCGaat                                 >  4:302233/1‑149 (MQ=255)
                                                                                                                       cccGGTCGC‑TGTTCCATTTTTCGCGCTTTGGCTCGGGCAATAGCCGCTTCTACGGCGTGACTGCCGTGAACCCGCTGTTTCTCCCGATCTG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGAcgg                                <  3:447294/150‑1 (MQ=9)
                                                                                                                            ttGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGAGCTCGTAGGCGGCGTCTTCT‑GCAGGC‑‑CAGTGACGCGGTAGCTGTTCCGGCTTGCGGGCTTTGGCACGGTCAATAGAGGCTTGGCcggggggg                           >  1:804624/1‑149 (MQ=2)
                                                                                                                              gC‑TGTTCCGGTTTTCGGGCGTTTGCGCGGGCAATAGCCGCGTCTACGGCCGCTTTGCGCGGATCTACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCttt                         <  8:150467/151‑1 (MQ=14)
                                                                                                                                       cgattGCGCGCTTTGGCTCGTGCAATAGCTGCTTCGACTGCGGCTTTGCGCGGATCTACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGAt                 <  4:345321/148‑1 (MQ=255)
                                                                                                                                       cGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCGG‑‑CA‑TTAGCGTGTTGATGTTCCCGCTTGGGAGCTGTGGGCCGTGCGGTGGCGGGGTGGGCGGGGGCTTTGCGGGGAt                 >  7:115520/1‑151 (MQ=11)
                                                                                                                                        gTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGATTTGCGCGGATCGCCCTGGTGTTCTGGGTCGG‑‑CA‑TTAGCCTGTTGGTGTTCGAGCTGGCGTGCTATGGCCCGGCCCATAGCTGCTTCGACGGCGGGTTTCCGGGGATc                >  7:837755/1‑151 (MQ=14)
                                                                                                                                         gttGCGCGCGTGGGCGCGGGCAATAGCGGCTGCTACGGCGGCTTGGCGCGGAGCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCg               <  3:204066/150‑1 (MQ=14)
                                                                                                                                         attgcgaggtgttccTCGGTCATTACCTGCGTCGACGTCCGCTTTGCGCTTATCGACGTGCTTGTCTGGTACCG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATc                <  6:420804/136‑1 (MQ=2)
                                                                                                                                             cgcgcTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGGTTTGCGCGTATCGACCTGTGCGTGGGGATCTG‑‑CA‑TTAGCGGCTTGCTGTTCCAGCTTGCGGGCTTTGGCTCGGGCAATAGCAGCTTCGACGGCGGGTTTGCggggcgggcgcg           >  1:69436/1‑143 (MQ=2)
                                                                                                                                               tgcTTTTGCGCGGGCAATAGCCGCTGCTACTGCTGCTTTGCGCGTATCTCCCTGTTCTTCTGGGTCGG‑‑CA‑TTAGCCTGTTGCTGTTCGAGGTTGCGTGCTTTTGCTCGGGCAATAGCGGCTTCGACTGCGGCTTTGCGCGGATCGACCTg          <  4:390159/149‑1 (MQ=11)
                                                                                                                                                 cTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGGTTTGCGCGGATCGCCATGTTTTTCTGGGTCGG‑‑CA‑TTATCATGTAGCTGTTCCAGCTTTCTGGATTTGGCTCGCGCCATAGCTGCTTCGACGGCGGGTTTGCGAGGATcaaaaaattt       >  3:326497/1‑142 (MQ=2)
                                                                                                                                                    tGGCGCGGGCACGCGCGGCTTGGACGGCAGCCGTGGGCTGATCCCAGCGTTCTTCTGGCTCCG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGttctt     <  5:608636/150‑1 (MQ=11)
                                                                                                                                                      gCTCGGGCAATAGCGGCTTCGAGGGCGGCTGAGCGCGGATCGCCCGGTTGCTCTGGCTCGG‑‑GA‑TTAGCCGGTTGCTGTTCCAGCTTGCGGGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTg  <  3:156618/151‑1 (MQ=14)
                                                                                                                                                        |                                                                                                                                                       
CTGTTCTTCTGGTACCGCATTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGC‑GCGGATCAACCTGTTCTTCTGGCTCCGCATTAGCCGGTTGC‑TGTTCCCGTTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGG‑‑CA‑TTAGCCTGTTGCTGTTCCAGCTTGCGTGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTG  >  DH1/2169427‑2169725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑