Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	3,184,585	0	C	A	27.9%	159.3 / 24.3	81	intergenic (+8/‑30)	ECDH1_RS15795/ECDH1_RS15800	tRNA‑Gln/tRNA‑TERM
Reads supporting (aligned to +/- strand):  ref base C (28/29);  new base A (20/2);  total (49/32)
Fisher's exact test for biased strand distribution p-value = 6.46e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184436‑3184734                                                                                                                                                      |                                                                                                                                                      gAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCa                                                                                                                                                      <  8:221631/151‑1 (MQ=255)   aTCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAg                                                                                                                                                    <  6:944359/151‑1 (MQ=255)     cccGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGa                                                                                                                                                   <  6:735000/150‑1 (MQ=255)     cccGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAc                                                                                                                                                  <  8:14804/151‑1 (MQ=255)          cgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTATAAAAGatccctt                                                                                                                                             >  8:558305/1‑145 (MQ=21)          cgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTCATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGaaccat                                                                                                                                              >  8:56196/1‑145 (MQ=255)            tAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTCCAGAAGATTAc                                                                                                                                            >  7:23965/1‑150 (MQ=255)               ccaccaCATGATGAATGTATCCAATAGGAGCGGCACATTCAACCCCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTg                                                                                                                                        <  3:933121/151‑1 (MQ=21)                 accaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTa                                                                                                                                      <  6:429021/151‑1 (MQ=255)                  acaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGTGTATCCCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTaa                                                                                                                                     <  3:942659/150‑1 (MQ=255)                   caAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAg                                                                                                                                    <  3:879698/151‑1 (MQ=255)                   caAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAg                                                                                                                                    <  3:874461/151‑1 (MQ=255)                    aaaTTCTGAATGTATCTAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTAGCGCAGGAGAGTTGTTGAAGAAGCTTCACTTGTaggg                                                                                                                                   >  8:944066/1‑148 (MQ=21)                       ttCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAGATGGCTACGTTGtcatttat                                                                                                                                >  1:328008/1‑144 (MQ=18)                           gAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGT‑AGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCAccc                                                                                                                             <  7:798579/149‑1 (MQ=255)                            aaTCTATCCAATATGTTCTGCAAATTCAAACCCAATTTGTTTGTGTATCTCCAAGCCGTAATGCACCTGATTCTGATTCCGGCATTCCTAGTTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAg                                                                                                                           <  2:57325/151‑1 (MQ=25)                                     aaTATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTa                                                                                                                   <  6:66831/150‑1 (MQ=255)                                      atatTTTCGGAAAATTAATATCTTCTTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATc                                                                                                                 <  4:173052/151‑1 (MQ=21)                                             cGGCAATTTCAAATCGAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAg                                                                                                           <  6:1056179/150‑1 (MQ=255)                                                aaaaTTCAATACAAATTTGTTGGGTTATCGCAAAGCTGTAAGGCAACGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTTTAAGTGCACCGAGTTGGGGTATCGCCAAGCGGt                                                                                                       <  6:92542/150‑1 (MQ=25)                                                 ataTTCAAAACCACTTTGTTTGTGTATCTCCAACCTTTAAGGAACATGATTCTGTTTCAGGCTTTACGAGGTTCGAATCCTCGTAACCCAGACAATTTATTAAAGACGCTTAAATTTTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTa                                                                                                      <  4:616323/149‑1 (MQ=255)                                                   aTTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCACCGTACCTGGTAAGTGTACCGATTGTGGGTACCTGCAAGGGGTa                                                                                                      >  8:594459/1‑149 (MQ=11)                                                     tCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGAGGCGTACCTTGTTAGTGGAGCTATTTGGCGTTTCGCCAAGCGGTAAGGc                                                                                                  >  7:929/1‑151 (MQ=18)                                                      cAAAACCAGTTTGTTTGGGTCTTTCAACGCTGTAAGGCACCTGATGCTGTTTCCGGCACGCATCGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTAACTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGc                                                                                                  <  5:722068/150‑1 (MQ=255)                                                          aCCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGCTTACCTTGTAAGTGCACCAAGTTTGGGTATCGCCAAGAGGTAAGGCAACgg                                                                                             >  3:177133/1‑151 (MQ=25)                                                           cccgtttATTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCt                                                                                                                                          <  4:814400/101‑1 (MQ=255)                                                           cccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAGTTATTAAAGACGGTTACCt                                                                                                                                          >  3:814749/9‑105 (MQ=11)                                                              aTTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAGGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCTGTAAGGCACCGGATTc                                                                                         >  4:1070540/1‑151 (MQ=255)                                                                  attgGGGTATCGCCAAGCGGTAAGGCACCGGTTTGTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTg                                                                                                                        >  2:1067031/2‑116 (MQ=255)                                                                  attgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTg                                                                                                                        <  1:1067424/115‑1 (MQ=255)                                                                     ggggTATCGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCg                                                                                                                >  8:339805/1‑121 (MQ=255)                                                                     ggggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATTCAAGCCGCTTACCTTGTGAGTGCTCCCTGTTGGGGTATCGGCAAGCGGGAAGGCTCCggtgtctgcttgc                                                                                  >  7:548598/1‑140 (MQ=18)                                                                     ggggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCg                                                                                                                <  7:340160/121‑1 (MQ=255)                                                                          aTCGCCAAGCGGTAAGGCACCGGTTTGTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGt                                                                                                                                   >  2:103779/1‑97 (MQ=255)                                                                          aTCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGGTACGGAGTGCTTTATCCGAGTTGGCGTATCGCCAAGCGGTAAGGCGCCGGagacaatttactgcat                                                                             >  5:907462/1‑136 (MQ=9)                                                                          aTCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGt                                                                                                                                   <  1:103841/97‑1 (MQ=255)                                                                             gCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGCCCCTTACCTTGTAAGTGAACCGAGTTGGGGTATCGCCAAGCGCTAAGGCACCGGAGTCTAATTCCGGCCTTcc                                                                          >  1:186456/1‑151 (MQ=25)                                                                                aaGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAATTTATTc                                                                                                                                                       >  3:27013/1‑71 (MQ=1)                                                                                    cgcaacccACCGGATGCTGATACCGGCATTCCGAGGGTCGAATCCTCGTACGCCAGACAATTTATTCAAGCCGCTTACCTTGTAACTGCACGGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTc                                                                   <  1:554553/144‑1 (MQ=25)                                                                                     tgaagcctccGGACTCTGTTACCGGCATTCCGCGCTTCGAATCCTCCTACACCATCATATTTATTCAATACTCTTAACTTGTAAGTGCACCGAGTTGGGGTTTCGCCAAGCGGTAAGGCACCGGATTCTTATTCCGGCATTCCGAgg                                                                      <  6:603099/139‑1 (MQ=18)                                                                                      cacgcccccGGATGCGGATACCGGCACTCCGCGCTTCGAATCCTCGTACCCCAGACAATTTATTCAAGCCGCTTACCTTGTAATTGCAACCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGa                                                                 <  5:659613/144‑1 (MQ=21)                                                                                          cctaaggATTCTGTGTCCGGCATTAATCGGGTCGAATCCTCGTAACCCTGCATATTTCTTATATTCTCGTCCCATGTAATTCCACTTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATcc                                                             <  3:1032659/146‑1 (MQ=2)                                                                                           cACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATCAAGATCCGTACCTTGAAAGTGCACCGAGTTTGGGTATCTACAATGTCTAATGCTACGGATACTAATTGCGGCATTcaatgttgcgattccc                                                            >  7:460227/1‑136 (MQ=12)                                                                                           cACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAGAAGCATACGTAGTAAGTTCACCCAGTGGGGGTATCGCGAATCGCTAAGGCTCAGGATTCTAATACGAGCATTCAGAGGttgcaaacc                                                             >  1:515030/1‑143 (MQ=16)                                                                                           aacaggATTGTGACTCAGGCATTACTAGCTTCGAAGCCTCGAAAGCCTGACACTTTATTCTATCCGCTTACATTGTAATTGCAATCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCt                                                            <  8:391440/147‑1 (MQ=9)                                                                                            aaccgATCCTGACACCGGCACTCCCACCTTCGAATCCTCGTACACCTTAAAATTTATTCTAGCCGCTTACCTTTTAACTGCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTc                                                           <  6:559947/147‑1 (MQ=17)                                                                                             ccGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAGGCTTACCTTGTAAGTGAACCGAGTTGGGGTATAGACAAGCTGTAAGGCGCCGGTTTCTAGTTCCGTCATTCCGAGGTTCgtctccggg                                                          >  3:196238/1‑143 (MQ=25)                                                                                                 tttttGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCaa                                                                                                                                                     <  2:670387/52‑1 (MQ=17)                                                                                                    cTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAAAAAAGCAGCTTACGTGGTAAGTGCACCCATTTGGGGTATCGCCAAGCGGTAAGGCACGGGAGTCTAATTCCGGCATTCAGAGTATCGAAACCGCGTAccgcc                                                   >  7:460903/1‑148 (MQ=21)                                                                                                      gATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGAACCGTACCTGGTGAGTGGACCCAGTTGGGGTAGCGGCAAGGGGTAATGCCACGTAGTCTACTTGCCTCATTCCGAGTATCGAGACCACGTCccccctc                                                 >  7:880091/1‑148 (MQ=9)                                                                                                          ccGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTACGGCATTCCGAGTTTCGAATCCTCGTACCCCAGCcac                                              >  5:1039812/1‑150 (MQ=255)                                                                                                                  ccGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAGCAGCTTACCTTGTTAGTGATCCGAGTAGGGGTAAAGCCAATCTCTAAGGCACAGTTTTCTACTTAATTCATTCAGAGTCTCGACTCAAGGTCCCCCCGCAACATTaaaaa                                      >  5:720375/1‑150 (MQ=255)                                                                                                                   cGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGAAGCTTTCCTTGTAAGTGCAGCGATTTGGGGTAGAGACAATCTCTAATGCGCGGGTTACTAATTGCGTCATTCAGAGTGTCGACTCCGCGTACCGCAGCCGCATTaaaaaat                                    >  5:713859/1‑150 (MQ=255)                                                                                                                   cGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGAAGCATACCTAGTAAGTGAACCAATTAGGGGTATATCCAAGCGCTAATGCAACGTATACTACTAAAATCATTCAGAGTATCCAATCCTCCTACCCCAGCGAAAATaaaaaac                                    >  8:892711/1‑150 (MQ=255)                                                                                                                    gAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATAGACAAGCGGTAATGCACCGGATTCTACTTGCTGCATTCCGAGGATCGAATCCTCGTACCCCAGCCACATTAAAAAAGc                                   >  1:802592/1‑151 (MQ=255)                                                                                                                     aGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGTTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCt                                  >  3:122344/1‑151 (MQ=255)                                                                                                                          cGAATCCTCGTACCCCAGCCAATTTATAAAAGACCCATACCTGGTACGTGCAGCCAGTTGGGGTATCGCCAATCGGTAAGGCACAGGAGTCTAATTCAGGCATTCCCAGTATCGAAGCCTCGTGCCCCAGCGACATTAAAAAACCTCGCtt                             >  5:712786/1‑151 (MQ=255)                                                                                                                          cGAATCCTCGTACCCCAGCCAATTTAATAAAGAGGCTTACCTAGTAAGTTAACCAAGTTTGCGTATCTACAATCTGTAATGCACAGGATACTAATTAAATCATTCAGTGTATCAAATACTACTAAACCAGCAACATTAAAAAAGCTCTCtt                             >  8:155659/1‑151 (MQ=255)                                                                                                                           gAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCATCGTACCCCAGCCACATAAAAAAACCTCGCTTc                            >  4:188028/1‑151 (MQ=255)                                                                                                                           gAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            >  2:994192/1‑151 (MQ=255)                                                                                                                           gAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGACAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGATCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            >  3:95762/1‑151 (MQ=255)                                                                                                                            aagccccctACCCCCGCCAATTTATTTTATTATCTTAACTTGTAACAGCTAGCTGTTGTGGTATCTCCAATCTGTTAGGCAACTGATTCTTATTCCGTCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCg                           <  2:101182/143‑1 (MQ=9)                                                                                                                            aaTCCTCGTACCCCAGCCAATTTATACAAGCACCTTACCTTGTAAGTTAACCCAGTAGGCGTAACGACAAGCTGGAATGCACAGGATACTCATTCGGGCATTCAAAGGATCGAAGCAACGTGCCCCAGCGACAATAAAAAAGCTAGCTACg                           >  6:682269/1‑151 (MQ=255)                                                                                                                               ccTCGTACCGCAGCCAATATATTCAAGCCGCTTCCCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGc                         <  3:429372/150‑1 (MQ=255)                                                                                                                                   gTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGGTTCGGCGAGCt                    >  7:586410/1‑151 (MQ=255)                                                                                                                                    tACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtt                   >  5:866935/1‑151 (MQ=255)                                                                                                                                    tACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtt                   >  3:588962/1‑151 (MQ=255)                                                                                                                                    tACACCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGAGGTAAGGCACCGGATTCTAATTCCGTCATTCCTAGGTTCGAATCCTCTAACCCCAGCCACATTAAAAAAGCTCGCTTCGGCTAGCtt                   >  3:1055824/1‑151 (MQ=255)                                                                                                                                     aCCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  7:720731/1‑151 (MQ=255)                                                                                                                                     aCCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  5:450165/1‑151 (MQ=255)                                                                                                                                        ccAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTg               >  2:449305/1‑151 (MQ=255)                                                                                                                                        ccAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTg               >  5:609459/1‑151 (MQ=255)                                                                                                                                         cAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGc              >  6:180180/1‑151 (MQ=255)                                                                                                                                          aGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt             >  8:392693/1‑151 (MQ=255)                                                                                                                                            ccAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCttt           >  3:462605/1‑151 (MQ=255)                                                                                                                                               ctatcttCAAGCCGCGTCCCATGCAACAGCAACGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          <  3:125220/144‑1 (MQ=18)                                                                                                                                                tttATTCAAGACGCTTACCTTGTAAGTGCACACAGTTGGGGTATCGACAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCATTTCTg       >  4:134960/1‑151 (MQ=255)                                                                                                                                                 ttATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGc      >  7:298912/1‑151 (MQ=255)                                                                                                                                                  tATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     >  6:241204/1‑151 (MQ=255)                                                                                                                                                  tATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     >  1:136996/1‑151 (MQ=255)                                                                                                                                                     tCAAGTCGGTTACCTTGTAATTGCACTCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGtt   <  4:177118/150‑1 (MQ=255)                                                                                                                                                     tCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  6:462688/1‑151 (MQ=255)                                                                                                                                                      cAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  3:456161/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                      GAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184436‑3184734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑