Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,447,4030AC24.1% 410.5 / 39.5 209G346G (GGT→GGG) malZalpha‑glycosidase
Reads supporting (aligned to +/- strand):  ref base A (49/108);  new base C (50/0);  total (100/109)
Fisher's exact test for biased strand distribution p-value = 1.55e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447254‑3447552
                                                                                                                                                     |                                                                                                                                                     
ccACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCAc                                                                                                                                                      <  3:874077/151‑1 (MQ=255)
  aCGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCAccc                                                                                                                                                    <  7:73270/151‑1 (MQ=255)
   cGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCg                                                                                                                                                   <  5:472413/151‑1 (MQ=255)
     aTAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGcc                                                                                                                                                 <  8:798957/151‑1 (MQ=255)
      tAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGCTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGccc                                                                                                                                                >  4:168411/1‑150 (MQ=255)
        gTTCATGGCGGCATCTACCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTcc                                                                                                                                              <  2:678665/151‑1 (MQ=255)
         ttCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTccc                                                                                                                                             >  1:500566/1‑151 (MQ=255)
             tGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGAGAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTGCCCCa                                                                                                                                          >  4:156090/1‑150 (MQ=255)
              ggcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGc                                                                                                                                        <  6:199654/151‑1 (MQ=255)
                 ggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCata                                                                                                                                     <  4:407264/151‑1 (MQ=255)
                 ggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCat                                                                                                                                      >  1:84057/1‑150 (MQ=255)
                  gcttCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATCTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCatat                                                                                                                                    <  2:687162/148‑1 (MQ=255)
                  gcATCTTCCACATCGGCCTGTAACCGTTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCatat                                                                                                                                    <  7:618460/151‑1 (MQ=255)
                   cATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCACCaaatt                                                                                                                                   >  2:500715/1‑150 (MQ=255)
                    aTCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCCGCatattc                                                                                                                                  >  2:529302/1‑149 (MQ=255)
                        tCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCTCCCCCCCCCTATGcaccc                                                                                                                              >  7:667874/1‑150 (MQ=255)
                        tCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCCGCATATGcaccc                                                                                                                              >  1:384942/1‑150 (MQ=255)
                        tCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGc                                                                                                                                  <  5:994527/147‑1 (MQ=255)
                         ccACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCACCCCCACCATATGcccccc                                                                                                                             >  3:881873/1‑149 (MQ=255)
                          cacaTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACcaca                                                                                                                            <  7:564919/151‑1 (MQ=255)
                             aTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATcc                                                                                                                         <  7:85247/151‑1 (MQ=255)
                               cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccag                                                                                                                       <  7:1059669/151‑1 (MQ=255)
                                 gCCTGTAACCATTGCCGTGCATCGCGAAAATGATCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagcc                                                                                                                     <  8:923783/151‑1 (MQ=255)
                                 gCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagcc                                                                                                                     <  4:53556/151‑1 (MQ=255)
                                   cTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCCCCACAAccagccgc                                                                                                                   >  5:913006/1‑151 (MQ=255)
                                    tGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGCATATGCACCACCTccagccgcc                                                                                                                  >  3:47825/1‑151 (MQ=255)
                                    tGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACAAccacccgcc                                                                                                                  >  7:530871/1‑151 (MQ=255)
                                     gTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgcca                                                                                                                 <  7:95840/151‑1 (MQ=255)
                                      tAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgcccc                                                                                                                >  7:246389/1‑149 (MQ=255)
                                       aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgcccgc                                                                                                               >  5:205123/1‑151 (MQ=255)
                                       aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgccagc                                                                                                               <  6:1034898/151‑1 (MQ=255)
                                       aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgccag                                                                                                                <  1:11219/150‑1 (MQ=255)
                                       aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATACCGGCAACGTGCTCCATATTATTGCTCGCGCCCCCCGCCACCCCCCCCAGTTGCCCCACCTccagccgcccgc                                                                                                               >  8:79130/1‑151 (MQ=255)
                                              gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCat                                                                                                        <  1:573571/151‑1 (MQ=255)
                                               ccGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCGCCCCCCGCAAATGCACCCCATCCACCCGCCAGCCGTCCat                                                                                                        >  5:525418/1‑150 (MQ=255)
                                                cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCGCCCCCACCATAAGCACCACATCCAGCCGCCAGCCGTCCatat                                                                                                      >  1:428614/1‑151 (MQ=255)
                                                cGTGCATCGCCAAAATCTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCatat                                                                                                      <  3:834688/151‑1 (MQ=255)
                                                 gTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCCCCCCCCACAAATTCCCCCCAAccagccccccccccgccaaatt                                                                                                     >  7:599883/1‑138 (MQ=255)
                                                  tGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCCCCATATTGACCACCAccacccgcccccccgccctatt                                                                                                     >  5:779071/1‑137 (MQ=255)
                                                  tGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCGCCCCCAGCATATGCACCACATACAGCCGCCAGCCGTCCATATTc                                                                                                    >  2:944719/1‑151 (MQ=255)
                                                  tGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCGCCCCCAGCCTATGCACCCCCTCCAGCCGCCCGCCGTCCATATTc                                                                                                    >  1:980377/1‑151 (MQ=255)
                                                     aTCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCAc                                                                                                 <  7:117019/151‑1 (MQ=255)
                                                      tCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCGCATATGCACCACATCCAGCCGCCAGCCGGCCATATTACACg                                                                                                >  7:25595/1‑151 (MQ=255)
                                                         ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgcg                                                                                             <  8:200203/151‑1 (MQ=255)
                                                         ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgcg                                                                                             <  2:880263/151‑1 (MQ=255)
                                                         ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgcg                                                                                             <  5:137128/151‑1 (MQ=255)
                                                         ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCGCCCCCCCCAAATGCCCCCCCAACACCCCCCACCCCTCCATAATACACCgcc                                                                                             >  1:1073852/1‑150 (MQ=255)
                                                           aaaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCCCCCCCCACCCCCCGCAAATGCACCCACTCCACCCCCCCCCCGTCCAAATTCCACCGCGCt                                                                                           >  7:887101/1‑151 (MQ=255)
                                                                gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGGCCCCCGCCTATGCCCCACATGCAGCCGCCCCCCGGCCAAATTTCACCGCGCttta                                                                                        >  4:174902/1‑148 (MQ=255)
                                                                  tCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGc                                                                                     <  5:937470/150‑1 (MQ=255)
                                                                   cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATCCACCAACTACAGCCGCCAGCCGTCCAAAATCCACCGCGCTTTCAGcc                                                                                    >  5:426753/1‑150 (MQ=255)
                                                                     ccGACAATATACGCTTCCGGCGGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGt                                                                                 <  5:1063506/151‑1 (MQ=255)
                                                                       gACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTgg                                                                               <  6:653716/151‑1 (MQ=255)
                                                                           atatACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCCGCAAATGCCCCCCCTCCAGCCGCCAGCCCTCCAAATTACACGGCCCTGTAAGCCACTGCCggc                                                                           >  6:607251/1‑150 (MQ=255)
                                                                           atatACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCACCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCGTTCAGCCAGTGGCggc                                                                           >  4:953493/1‑150 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCACCATATGCCCCACATCCAGCCGCCCGCCGTGCATATTGCACCGCGCGTTGACCCAGTGGCGGAc                                                                          >  1:729837/1‑151 (MQ=255)
                                                                            tataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATTCACCAAATACAGCCGCCCTCCGGCCATATTACAAGGCGCATTAAGCCAGTGGCGGa                                                                           >  3:633871/1‑150 (MQ=255)
                                                                             ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGAc                                                                          <  2:18608/150‑1 (MQ=255)
                                                                             ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACa                                                                         <  6:894372/151‑1 (MQ=255)
                                                                                    tCCGGCTGGGTTTCTTTCGCCGCGTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGTCAATACTg                                                                   <  5:77092/150‑1 (MQ=255)
                                                                                     ccGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATTCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTc                                                                 <  7:949171/151‑1 (MQ=255)
                                                                                     ccGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGtg                                                                 >  2:464759/1‑150 (MQ=255)
                                                                                      cGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCt                                                                <  6:816635/151‑1 (MQ=255)
                                                                                          tGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGCATATGCACCCCCTCCACCCGCCCGCCGTCCATATTCCACCGCGCGTTGCGCCAGTGGCGGCCAAAACTGTCTTccc                                                            >  8:1042546/1‑151 (MQ=255)
                                                                                          tGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACCTCCAGCCGCCACCCGTCCATATTCCACGGCGCGTTAAGCCAGTGGCGGACAATACTGTCTTccc                                                            >  4:677517/1‑151 (MQ=255)
                                                                                             gTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCGCCCCCCGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCACTGGCGGACAATACTGTCTTCCCcgc                                                         >  5:1019814/1‑151 (MQ=255)
                                                                                                  tttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCCGTGGCGGACAATACTGTCTTCCCCGCGATa                                                     >  3:617533/1‑150 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaa                                                    <  6:518117/150‑1 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaa                                                    <  8:1080267/150‑1 (MQ=255)
                                                                                                    tcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCACCATATGCCCCACATCCAGCCGCCACCCGTCCATATTCCACGGCGCGTTGAGCCAGTGGCGGACAATACTGTCTTCCCCCCGATaaa                                                   >  3:362037/1‑150 (MQ=255)
                                                                                                    tcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATaaa                                                   <  8:149732/150‑1 (MQ=255)
                                                                                                     cgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCGTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAtt                                                 >  2:1016426/1‑151 (MQ=255)
                                                                                                       ccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttc                                               <  8:743213/151‑1 (MQ=255)
                                                                                                        cgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttca                                              <  1:529773/151‑1 (MQ=255)
                                                                                                        cgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttca                                              <  1:442436/151‑1 (MQ=255)
                                                                                                               gTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcattca                                          <  8:702914/148‑1 (MQ=255)
                                                                                                               gTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCa                                       <  8:530257/151‑1 (MQ=255)
                                                                                                                 gATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAg                                      <  8:256305/150‑1 (MQ=255)
                                                                                                                  aTCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGGCTCCCCCACCAAATTGACCACAACCAGCCGCCCCCCCGCCATATTAAACCGCGCGTTAAGCCAGTGGCGGCCAAATAAGGCTTGCCCGCGAGAAATTTAAATCACCacac                                    >  5:868465/1‑151 (MQ=255)
                                                                                                                      cGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTc                                <  4:47858/151‑1 (MQ=255)
                                                                                                                       ggCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTcc                               <  4:383647/151‑1 (MQ=255)
                                                                                                                         cAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGa                             <  4:257736/151‑1 (MQ=255)
                                                                                                                         cAACGTGCTGCATATTATTGCGCACCCCCCCCCCCGCCCCCAGCCTATGCCCCCCCTCCCGCCGCCCCGCGGCCCAATTACCCGGCGCTTTGAGCCAGGGGCGGGCCATTAAGTCGTGCCCGCGCTAAATTTAAATGACCCGACGTTGCgc                             >  8:587773/1‑150 (MQ=255)
                                                                                                                            cGTGCTGCATATTATTGCGCGCCCCCCCCCCCTCCCCCACCATATGCACCCCATCCCGCCGCCAGCCGGCCATATTGCACCGCCCGTTCAGCCAGTGGCGGGCAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACATTGCCACTg                          >  7:291211/1‑151 (MQ=255)
                                                                                                                                  gcATATTATTGCGCGCCCCACCCGCCGCCCCCCGCATATGCACCACATACACCCGCCAACCCTCCATATTCCACCGCGCTTTAAGACAGAGGCCGACAAAAAAGTCTTACCCGCGATAAATTTCATTAACAAGAATTTACGACTGATaaa                     >  2:268330/1‑149 (MQ=255)
                                                                                                                                   cATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATcc                   <  4:641542/151‑1 (MQ=255)
                                                                                                                                     aattattGCGCGCCCCACCCGCCTCCCCCAGCATATCCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAg                 <  4:277152/150‑1 (MQ=255)
                                                                                                                                      attattGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAg                 <  2:1078280/150‑1 (MQ=255)
                                                                                                                                      attattGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGc                >  2:993576/1‑151 (MQ=255)
                                                                                                                                           tGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGACAATACTGTGTTCCCCGCGATAAATTTCATTCACCAGCCTTTCCGCCTGATAATCCAGCTTCgg           >  4:604262/1‑151 (MQ=255)
                                                                                                                                            gcgcgcCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGc          <  2:839398/151‑1 (MQ=255)
                                                                                                                                                 ccccTCCCGCCTCCCCGAGCATATGCACCCCATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTGCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCt     <  4:10898/151‑1 (MQ=255)
                                                                                                                                                 ccccACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCt     <  3:156037/151‑1 (MQ=255)
                                                                                                                                                  cccACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTg    >  1:90154/1‑151 (MQ=255)
                                                                                                                                                  cccACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTg    <  6:777912/151‑1 (MQ=255)
                                                                                                                                                  cccACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTg    <  6:62725/151‑1 (MQ=255)
                                                                                                                                                  cccACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTg    >  2:796534/1‑151 (MQ=255)
                                                                                                                                                    cACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc  <  1:798343/151‑1 (MQ=255)
                                                                                                                                                     aCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc  >  3:223096/1‑150 (MQ=255)
                                                                                                                                                     aCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc  >  3:96001/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447254‑3447552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑