BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	3,448,523	0	A	C	25.6%	502.4 / 47.7	243	intergenic (‑83/+73)	malZ/proY	alpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand): ref base A (132/48); new base C (0/62); total (132/111)
Fisher's exact test for biased strand distribution p-value = 1.08e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

GAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGA  >  DH1/3448375‑3448672

gAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggag                                                                                                                                                      <  5:134374/150‑1 (MQ=255)

gAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggag                                                                                                                                                      <  3:488273/150‑1 (MQ=255)

gAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggag                                                                                                                                                      <  8:231195/150‑1 (MQ=255)

 aGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggagga                                                                                                                                                    <  3:536967/151‑1 (MQ=255)

 aGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggagga                                                                                                                                                    <  3:795991/151‑1 (MQ=255)

   cAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACg                                                                                                                                                  <  7:802010/151‑1 (MQ=255)

      ttGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgggg                                                                                                                                               >  6:216948/1‑151 (MQ=255)

      ttGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgggg                                                                                                                                               >  3:289594/1‑151 (MQ=255)

       tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggggg                                                                                                                                              >  1:659138/1‑151 (MQ=255)

          tCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTACCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGTGGAGGAGGACGGGGGCgg                                                                                                                                           >  2:1063463/1‑151 (MQ=255)

             ttgctttgTATAACAACGGGGGCCACCGGCAGGTGCCTGTCATTTAACATCATAATTCCCCTGTGATTAAAATCTCATCACCTTGCCATAGCCTACTGCAGGATCACTCATCATCTCATTTATATTGGGGGGCGGCGGCCGGGGGCGGAt                                                                                                                                         <  8:684302/150‑1 (MQ=255)

              ttctttgTTTAAAAATTGGGGGAACCGGCTGGTGACATGATTTTAACATCATAATTCCCCTTTTATGAAAATCTTATCACCTTGCCATAGCCTAATGCTGGATCACTCATCATCTGAAGGATTTTTGGGGGAGGAGGACGGGGGCAGATa                                                                                                                                        <  3:101880/148‑1 (MQ=255)

              tggtttgTTTAACAACTGGGCGCACCGGCAGGTGCCATTAATTTAAAATCATAATTCCCCTGTTATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATa                                                                                                                                        <  4:550388/147‑1 (MQ=255)

               tctttgTTTAACAAATGGGGGCACCGGCAGGTGCAATGAATTTAACATAATAATTCCCCTGTTATTAAAATCTTATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAATGATATTTGGGGGAGGAGGACGGGGGCGGATaa                                                                                                                                       <  7:153337/149‑1 (MQ=255)

               gctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAAc                                                                                                                                      >  3:180189/1‑151 (MQ=255)

                  ttgTTTAGCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACttt                                                                                                                                   >  7:494463/1‑151 (MQ=255)

                       tAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGa                                                                                                                               >  5:1014004/1‑150 (MQ=255)

                         aataaTGGCTGCCCCGGCACGTGCCATTAAATTATATTAATAATTCCCCTGTGATGATAATTTCTTCACATTTCATTAGCCTATTGCATTATAACTAATCATATTATTTATTTTTGGGGGCGGAGGACGGCGGCGGATAACTTTCGGAATa                                                                                                                            <  4:211879/148‑1 (MQ=255)

                          cAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCACCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATAACTCATCATCTGAAGGATATTTGGGGGAGGACTACGGGGGCGGATAACTTTCCGAAt                                                                                                                             >  1:480048/1‑149 (MQ=255)

                          aaaaTGGGGGCACCGGCAGGTGACATTCATTTAACATAATAATTCCCCTGTTATCAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGAGGAGGGGGGCGGATAACTTTCGGAATAg                                                                                                                           <  1:969953/150‑1 (MQ=255)

                               gggggCACCGGCAGGGGCCATGCATTTAACAACATAAATCCCGTGTTAGGAAAATCTGAGCACCTTGCCCTACCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGa                                                                                                                      <  3:412258/151‑1 (MQ=255)

                                  ggCACCGGCAGGTGCAATCAAATTAACAAAAAAATTCCCCTGTGATTAAAAGCTGATCACCTTCCCATAGCCTAATGCAGGATCACTCATCACCTGAAGGATATTTGGGGGCGGAGGACGGGGCCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                   <  7:626025/151‑1 (MQ=255)

                                      ccGGGAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATCAAAATCTGATCACGTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAt                                                                                                               <  1:884368/151‑1 (MQ=255)

                                       cGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAtt                                                                                                              >  2:885203/1‑151 (MQ=255)

                                         gCAGTTGCCATTCATTTAATATCATTTTTCCCCTTTGATGATATTGTGTTGACCTTGCCATCGCCTATTTCCGGATCTCTCTTCTTCTGCTGGCTTGGTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtc                                                                                                            <  4:701899/151‑1 (MQ=255)

                                             gTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGAGAACTTTCGGAATAGCCGGATCAGGATTtcctcc                                                                                                        >  1:553651/1‑151 (MQ=255)

                                               tccATGCATTAAACATCATAAGTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAAGGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTcctcct                                                                                                       <  3:254266/149‑1 (MQ=255)

                                               gCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                      >  3:59763/1‑151 (MQ=255)

                                                ccATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                      >  6:799134/1‑150 (MQ=255)

                                                 cagccATTTAACATCCTACTCCCCCCGTCAACAAACTCTGAGCCCCTCGCCCACCCCAACTCCAGGATCACTCCTCATCTGACCGATATTTGGCGGAGGCCGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGaa                                                                                                    <  6:1084456/147‑1 (MQ=255)

                                                   tGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGTTTTCCTCCTGAccc                                                                                                  >  7:254020/1‑151 (MQ=255)

                                                   tGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAcc                                                                                                  >  5:805582/1‑151 (MQ=255)

                                                      aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                               >  8:77587/1‑151 (MQ=255)

                                                      aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCAGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                               >  8:733081/1‑151 (MQ=255)

                                                       attAACAACATAATACCCCTGTGATCAAAAGCTGATCACCTTCCCATACCCTAATCCACCAGCACTCATCAGCTGAAGCATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCt                                                                                              <  6:28302/150‑1 (MQ=255)

                                                        ttAACATCATAAGTCCCCTGTGCTGAAACTGTGACCCCCTTGCCATCGCCTAATGCCGGATCACTCATCCTCTGCAGGATTTTTGGGGGCGGCGGCGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCt                                                                                              <  4:629968/150‑1 (MQ=255)

                                                           aaagcatAAGTCCCCTGTGATGAAACGCTGCACCCCGAGCCATACCCTACCGCCCGATCCCTCATCATCTGACGGCTATGGGGGGGCGGCGCCCGCCCCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACa                                                                                          <  3:520526/147‑1 (MQ=255)

                                                            catcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                           >  1:1011604/1‑149 (MQ=255)

                                                            aatcatAATTCCCCTGTGATCAAAATCTCATCACCTTGCCATACCCTAATGCAGGATCACTCATCATCTGATGGATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAg                                                                                         <  4:289763/150‑1 (MQ=255)

                                                             aacatAATTCCCCTGTGATCAAAACCTGACCCCCGTGCCAGACCCGAATCCAGGAACACACATCATCTGAAGGATATGGGGGGGCGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                        <  1:216413/149‑1 (MQ=255)

                                                             aacatAATGCCCCGGTGATGAAAATCTCCCCCCCTCCCCATAGCCTAATCCAGGATCACTCACCATCTGAAGGATATGGGGGGGAGCCGGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                        <  7:838254/149‑1 (MQ=255)

                                                              tcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                        >  5:293612/1‑150 (MQ=255)

                                                              ccaaaaagccccTGTGATGAAAATCTGAGCCCCTTGCCAGA‑CCTAACGCAGCATCCCTCACCATCTGACGGATATTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                      <  1:76299/143‑1 (MQ=255)

                                                              aaaaaaatCCCCTGTGAACAAAAACTCCGCACCTTGCCATAGCCTAATCCACGATCACTCCTCACCTGACGGCTCGTGCGCGGCCCCCGCCGCCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGaa                                                                                       <  5:485445/144‑1 (MQ=255)

                                                               caaaacgccccTCTGATGAAAATCTGAACACCTCGCCATAGCCTAATGCAGCATCACTCATCCTCTGAAGGATATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                      <  3:608305/144‑1 (MQ=255)

                                                               aatAATTCCCCTCTTATGAAAATCTGATCACCTTGCCATAGCCTAATCCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                      <  6:51468/150‑1 (MQ=255)

                                                                atAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACg                                                                                     >  8:102912/1‑151 (MQ=255)

                                                                   agaccccTGTGATCAAAATCTCATCACCTTGCCATCCCCTAATGCAGGACCACTCCTCATCTGAAGGATATTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCaa                                                                                  <  4:394965/148‑1 (MQ=255)

                                                                   aTTCTCCTTTGATTAAAATCTTCTCATCTTGCCTTATCCTACTTCTTGATCACTCTTCATGTGTTGTATTTTGGGGGGCGGCCGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCaa                                                                                  <  6:330973/151‑1 (MQ=255)

                                                                   aTTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTCGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCa                                                                                   >  5:165110/1‑150 (MQ=255)

                                                                       cccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGAt                                                                              >  7:701430/1‑151 (MQ=255)

                                                                          tgtgATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTAc                                                                           >  6:534055/1‑151 (MQ=255)

                                                                            tgatgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTg                                                                         >  2:80295/1‑151 (MQ=255)

                                                                                gaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGttt                                                                     >  8:487628/1‑151 (MQ=255)

                                                                                 aaaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAACGATTACTGGtttt                                                                    >  8:921635/1‑151 (MQ=255)

                                                                                 aaaaTCTGAACACCTTGCCATAGCCTAATGCACCATCACTAATAATCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGttt                                                                     <  4:100300/150‑1 (MQ=255)

                                                                                  aaaTCTCATCACCTTCCCATACCCTAATGCACGAGCACTCATCATCTGAAGTATATTTGGGGGAGGAGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                   <  5:405934/151‑1 (MQ=255)

                                                                                   aaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTTGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                   >  1:629877/1‑150 (MQ=255)

                                                                                    aTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                   >  5:263508/1‑149 (MQ=255)

                                                                                       tGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                               >  7:781185/1‑150 (MQ=255)

                                                                                        gtgcACCTTGCCATTGCCTATGGCAGCCTCAGTGACCCTCTGTAGTTTATGTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcca                                                              <  2:585640/147‑1 (MQ=255)

                                                                                        gATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                               >  2:158679/1‑149 (MQ=255)

                                                                                          tCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCt                                                           >  3:656474/1‑151 (MQ=255)

                                                                                          tCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCt                                                           >  5:58084/1‑151 (MQ=255)

                                                                                            aCCTTCCCATACCCTAATGCAGGATCACTCATCATCTGAAGGATATGTGGGGGAGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGa                                                         <  7:734550/151‑1 (MQ=255)

                                                                                              cTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg                                                       >  6:750104/1‑151 (MQ=255)

                                                                                                tcccctccccaaaTGCAGCACCACTCATCAGCTGACGGATATTTGGGGCCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg                                                       <  6:717120/138‑1 (MQ=255)

                                                                                                   cATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGt                                                  >  1:10301/1‑151 (MQ=255)

                                                                                                   cATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGt                                                  >  3:720567/1‑151 (MQ=255)

                                                                                                    aTAGCCTAATGCAGGATAACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACATTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTg                                                 >  2:485148/1‑151 (MQ=255)

                                                                                                         cTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                            >  8:182638/1‑151 (MQ=255)

                                                                                                         cTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                            >  2:966540/1‑151 (MQ=255)

                                                                                                         cTAATGCAGGATCACTAATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                            >  2:711203/1‑151 (MQ=255)

                                                                                                            aTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGtt                                         >  8:624791/1‑151 (MQ=255)

                                                                                                            aTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGtt                                         >  3:1043421/1‑151 (MQ=255)

                                                                                                             tGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGttt                                        >  6:1038017/1‑151 (MQ=255)

                                                                                                              gcatcctCACTCCTCATCTGCTGGCTATGCGGGCCAGGACGCCCCGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAGCGGCTTACAGAACGCAAGGATTACTGGGTTTCAGCCAGCTGACGATCGTGTCGGCGttt                                        <  5:775796/144‑1 (MQ=255)

                                                                                                               cAGGATCTATCATCAGCTGATTTTTATTTGGGGGAGGCGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTa                                       <  8:533612/150‑1 (MQ=255)

                                                                                                                cggATCACTCATCATCTCAAGTATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaaa                                     <  4:107493/150‑1 (MQ=255)

                                                                                                                 ggctCACTACTCCTCTCACGGTTATTTGGGGGCCGCGGCCGGGGGCGGATAACTGGCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAAc                                    <  8:1009856/148‑1 (MQ=255)

                                                                                                                   aTCAGTCATCATCTGAATGATTTTTGGGGGTGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACa                                   <  2:658768/150‑1 (MQ=255)

                                                                                                                   aTCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACAt                                  >  2:193620/1‑151 (MQ=255)

                                                                                                                         catcatCTGACGGATATTTGGGGGCGGAGGACGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAg                              <  7:1047507/149‑1 (MQ=255)

                                                                                                                          atcatcTGAAGGATTTTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCa                           <  2:353138/151‑1 (MQ=255)

                                                                                                                               ccgaaggattgggggggcAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCaa                      <  1:158624/138‑1 (MQ=255)

                                                                                                                                tGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                     >  1:703316/1‑151 (MQ=255)

                                                                                                                                tGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                     >  2:183491/1‑151 (MQ=255)

                                                                                                                                 gacggataggggggggAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                    <  3:935469/140‑1 (MQ=255)

                                                                                                                                 gacggATATTGGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                    <  7:1010319/148‑1 (MQ=255)

                                                                                                                                 gAAGGATATGGGGGGGCGGCCGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                        <  7:330011/147‑1 (MQ=255)

                                                                                                                                  aaGGATATTCGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                    <  8:451240/150‑1 (MQ=255)

                                                                                                                                   cggATCTTTGGGGGCGCCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc                  <  4:867799/150‑1 (MQ=255)

                                                                                                                                    ggATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc                  >  2:581443/1‑150 (MQ=255)

                                                                                                                                        atTTGGGGGAGGAGGACGGGGGCGGATAACTTTCAGAATAGCCGGATCAGGATTTCCTCCAGAACCGGCTTACAGAACGCAAGCATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaac             >  4:164774/1‑151 (MQ=255)

                                                                                                                                          ttGGGGGCGGCGGCGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca            <  4:544869/150‑1 (MQ=255)

                                                                                                                                          ttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca            >  4:875102/1‑150 (MQ=255)

                                                                                                                                           tGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAAt          >  2:137883/1‑151 (MQ=255)

                                                                                                                                           ggggggCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAAt          <  2:433441/150‑1 (MQ=255)

                                                                                                                                            gggggTGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATc         <  4:369218/151‑1 (MQ=255)

                                                                                                                                               ggaggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAc      >  1:380800/1‑151 (MQ=255)

GAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGA  >  DH1/3448375‑3448672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`