BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	3,448,526	0	A	C	30.9%	413.2 / 65.9	243	intergenic (‑86/+70)	malZ/proY	alpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand): ref base A (132/36); new base C (0/75); total (132/111)
Fisher's exact test for biased strand distribution p-value = 2.08e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676

aGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggagga                                                                                                                                                        <  7:934133/151‑1 (MQ=255)

  cAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACg                                                                                                                                                      <  3:524639/151‑1 (MQ=255)

  cAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACg                                                                                                                                                      <  7:802010/151‑1 (MQ=255)

    gTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggg                                                                                                                                                    <  3:72874/151‑1 (MQ=255)

      tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggggg                                                                                                                                                  >  1:659138/1‑151 (MQ=255)

      tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATATGAAGGATATTTGGGGGAGGAGGACggggg                                                                                                                                                  >  3:635766/1‑151 (MQ=255)

       gATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGc                                                                                                                                                 >  6:233250/1‑151 (MQ=255)

        aTCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATCAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGACGAGGACGGGGGCg                                                                                                                                                >  6:1051082/1‑151 (MQ=255)

         tCTTTGCTTTTTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCg                                                                                                                                                <  8:102851/150‑1 (MQ=255)

         tCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCgg                                                                                                                                               >  6:616941/1‑151 (MQ=255)

         tCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTACCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGTGGAGGAGGACGGGGGCgg                                                                                                                                               >  2:1063463/1‑151 (MQ=255)

            ttgctttgTATAACAACGGGGGCCACCGGCAGGTGCCTGTCATTTAACATCATAATTCCCCTGTGATTAAAATCTCATCACCTTGCCATAGCCTACTGCAGGATCACTCATCATCTCATTTATATTGGGGGGCGGCGGCCGGGGGCGGAt                                                                                                                                             <  8:684302/150‑1 (MQ=255)

             ttcttttTTTAACAACTGGGGCCACCGGCAGGTGCCATGAATTTAACATAATAATTCCCCTTTCATGAAAATCTGATCCCCTTGCCATAGCCTAATGCAGTATCACTCATCATCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATa                                                                                                                                            <  4:545776/148‑1 (MQ=255)

             tgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACATTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATa                                                                                                                                            >  4:920063/1‑150 (MQ=255)

              cctttgTTTAACAATTGGGCGCCCCGCCATTTTACATCAATTAAAAATCATAAATCCCCTCTCATGAAAATCTGATCACCTTCCCATAGCCTAATGCAGCATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGCCGCGGATaa                                                                                                                                           <  4:1063615/149‑1 (MQ=255)

               ctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACt                                                                                                                                         >  1:598522/1‑151 (MQ=255)

                tttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACtt                                                                                                                                        >  3:704711/1‑151 (MQ=255)

                 ttgTTTAGCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACttt                                                                                                                                       >  7:494463/1‑151 (MQ=255)

                  tttttAAAAATTGGGGGCACCGGCAGGTGCCATGCATTTAAAATAATAATTCCCCTTTTATGAAAATCTTATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATTTTTGGGGGAGGAGGACGGGGGCGGATAACttt                                                                                                                                       <  3:284000/148‑1 (MQ=255)

                       aaCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGCTCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                                 >  6:8059/1‑151 (MQ=255)

                         cAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCACCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATAACTCATCATCTGAAGGATATTTGGGGGAGGACTACGGGGGCGGATAACTTTCCGAAt                                                                                                                                 >  1:480048/1‑149 (MQ=255)

                         aaaaTGGGGGCACCGGCAGGTGACATTCATTTAACATAATAATTCCCCTGTTATCAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGAGGAGGGGGGCGGATAACTTTCGGAATAg                                                                                                                               <  1:969953/150‑1 (MQ=255)

                           aaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGcc                                                                                                                             >  4:343146/1‑151 (MQ=255)

                              gggcccACCGGCAGGTGCCATTAATTTAACATCATAATTCCCCTTTGATTAAAATCTGATCACCTTACCATAGCCTAATGCAGTATCACTCATCATCTTAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCgg                                                                                                                           <  5:229525/145‑1 (MQ=255)

                               gcggCAGCGGCAGGTGCAATGCCATAAAAATAATAATTCCCCGGTTTTGAAAATCTGATCACCTTGCCATACCATAATGCAGGATCACTCATCATCTGACGGTTATGTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGAt                                                                                                                         <  6:717658/149‑1 (MQ=255)

                                gggCACCGGCTGGTGCCATTCATTAAAAATAATAATGCCCCTGTTATTAAAATCTGACCACCTTGCCATCGCCTAATGCATGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATc                                                                                                                        <  3:178604/151‑1 (MQ=255)

                                gggCACCGGCAGGTGCCATTCATTTAAAATCATAATTCCCCTTTTATTAAAATCCGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATc                                                                                                                        <  5:376980/151‑1 (MQ=255)

                                 ggCACCGGCAGGTGCAATCAAATTAACAAAAAAATTCCCCTGTGATTAAAAGCTGATCACCTTCCCATAGCCTAATGCAGGATCACTCATCACCTGAAGGATATTTGGGGGCGGAGGACGGGGCCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                       <  7:626025/151‑1 (MQ=255)

                                    aCCGGCAGTTGCCATGCATTTAACATCATAATTCCCCTTTTATGAAAATCTCATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATAGTTGGGGGAGCCGGCCGCGGGCGGATAACTTTCGGAATAGCCGGATCAGGa                                                                                                                    <  3:499646/151‑1 (MQ=255)

                                     ccGGGAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATCAAAATCTGATCACGTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAt                                                                                                                   <  1:884368/151‑1 (MQ=255)

                                       gggaGGTGTCATTAATTTAACATCATAATTCCCCTGTGATGAAATTCTGATCACCTTGCCATAGCCTAATGCAGCATCACTCATCATCTTAAGTATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAttt                                                                                                                 <  7:77179/148‑1 (MQ=255)

                                       ggCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAttt                                                                                                                 >  3:4216/1‑151 (MQ=255)

                                        gaaGGTGCAATTCATTTAAAATCATAAATCCACTGTTATAAAAATCTGATCACCTTGCCATTGCCTTATGCAGGATCACTCATCATTTTCTTGTTTTTTGGGGGCGGCGCCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtc                                                                                                                <  3:1023966/149‑1 (MQ=255)

                                        gCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtc                                                                                                                >  7:650573/1‑151 (MQ=255)

                                            gTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGAGAACTTTCGGAATAGCCGGATCAGGATTtcctcc                                                                                                            >  1:553651/1‑151 (MQ=255)

                                              tccATGCATTAAACATCATAAGTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAAGGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTcctcct                                                                                                           <  3:254266/149‑1 (MQ=255)

                                               ccATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATAATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                          >  7:83190/1‑150 (MQ=255)

                                                 aTGCATTTAAAATCATAAATCCCCTGTGATGAAAATCTGATCCCCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTGGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAc                                                                                                       <  2:618512/151‑1 (MQ=255)

                                                  tGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGTTTTCCTCCTGAccc                                                                                                      >  7:254020/1‑151 (MQ=255)

                                                     aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                                   <  2:77629/151‑1 (MQ=255)

                                                     aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                                   >  8:77587/1‑151 (MQ=255)

                                                     aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCAGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                                   >  8:733081/1‑151 (MQ=255)

                                                      tttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCt                                                                                                  >  4:253358/1‑151 (MQ=255)

                                                      tttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTCCCATAGCCTAATGCAGGATCACTCATCATCTCAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCt                                                                                                  >  4:421142/1‑151 (MQ=255)

                                                       ttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCATCCTGAACCGGCtt                                                                                                 >  2:875959/1‑151 (MQ=255)

                                                           catcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                               >  1:1011604/1‑149 (MQ=255)

                                                            aacatAATTCCCCTGTGATCAAAACCTGACCCCCGTGCCAGACCCGAATCCAGGAACACACATCATCTGAAGGATATGGGGGGGCGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                            <  1:216413/149‑1 (MQ=255)

                                                             tcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                            >  8:243072/1‑150 (MQ=255)

                                                             tcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                            >  5:293612/1‑150 (MQ=255)

                                                             ccaaaaagccccTGTGATGAAAATCTGAGCCCCTTGCCAGA‑CCTAACGCAGCATCCCTCACCATCTGACGGATATTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          <  1:76299/143‑1 (MQ=255)

                                                             aaaaaaatCCCCTGTGAACAAAAACTCCGCACCTTGCCATAGCCTAATCCACGATCACTCCTCACCTGACGGCTCGTGCGCGGCCCCCGCCGCCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGaa                                                                                           <  5:485445/144‑1 (MQ=255)

                                                              catAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          >  5:797909/1‑151 (MQ=255)

                                                              catAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          >  5:701379/1‑151 (MQ=255)

                                                              catAATGCGCCTGTCATCAAACTCTGAGCCCCTCGCCATCGCCTACGGCAGGAGCCCCCATCCTCGGACGCATATTTGCGGCCGCAGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          <  3:573669/151‑1 (MQ=255)

                                                                   ttCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCa                                                                                       >  5:571701/1‑149 (MQ=255)

                                                                     ccccTTAGATTAACATCTGATCACCTTCCCATAGCACAAACAAGTATACCTCTTCATCTGAAGGATATGTGGGGGATGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGa                                                                                   <  4:389827/151‑1 (MQ=255)

                                                                      cccTGTGATGAAAAGCTGATCACGTTGCCATAGCCTAAGCCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGAt                                                                                  <  3:375352/151‑1 (MQ=255)

                                                                         tgtgATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTAc                                                                               >  5:972836/1‑151 (MQ=255)

                                                                             attaaaaGCTCATCACCTTGCCACAGCCTAATCCATCATCCCTCATCCTCTGAACTATTTTTGGGGCCCGCGTCCTGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGt                                                                           <  6:171852/148‑1 (MQ=255)

                                                                                aataTCTTATCACCTTGTCATACCCTAATTCATCATCTCTCTTCATCTGAAGGATATTGGGGGGCCGCGCCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGttt                                                                         <  6:518654/147‑1 (MQ=255)

                                                                                aaaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAACGATTACTGGtttt                                                                        >  8:921635/1‑151 (MQ=255)

                                                                                 aaaTCTGACCACCATCCCATAGCCTACTCCAGGCTCACTCATCATCTGACGGATTTTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                       <  8:500289/151‑1 (MQ=255)

                                                                                    tCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGCAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagc                                                                    >  2:11063/1‑151 (MQ=255)

                                                                                     ccgaccACCTTGCCATACCCTAATCCAGCACCACTCCCCCTCTGAAGGATATGGGGGGGCGGCGGCCGCGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                   <  2:977894/146‑1 (MQ=255)

                                                                                       gtgcACCTTGCCATTGCCTATGGCAGCCTCAGTGACCCTCTGTAGTTTATGTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcca                                                                  <  2:585640/147‑1 (MQ=255)

                                                                                       gATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                   >  2:158679/1‑149 (MQ=255)

                                                                                       gATCACATTGCCATCGCCTAATGCAGGTTCACTGATCATCTGAAGTATATTTGGGGGAGGCGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                     <  4:67377/147‑1 (MQ=255)

                                                                                         tCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCt                                                               >  4:175897/1‑151 (MQ=255)

                                                                                           aCCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTg                                                              >  6:484691/1‑150 (MQ=255)

                                                                                           aCCTTCCCATACCCTAATGCAGGATCACTCATCATCTGAAGGATATGTGGGGGAGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGa                                                             <  7:734550/151‑1 (MQ=255)

                                                                                            ccTTGCCATAGCCTAATGCATGATCACTAATCATCTGAAGGATATTAGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCAGGATCAGGATTTACTAATGAACAGGATTACAGAACGCAAGGATTACTGGTTTTCATCCAGCTGAc                                                            >  4:112144/1‑151 (MQ=255)

                                                                                                gCCATAGCCTAATCCAGGATCACTCATCATCTTAAGGATATTTGGGGGAGCCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATc                                                        <  3:604556/151‑1 (MQ=255)

                                                                                                  cATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGt                                                      >  1:10301/1‑151 (MQ=255)

                                                                                                    tAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTgg                                                    >  3:872941/1‑151 (MQ=255)

                                                                                                      cccTAATGCAGGAGCACTCATCATCGGCCGCATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcg                                                  <  7:145768/150‑1 (MQ=255)

                                                                                                        cTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                                >  4:59387/1‑151 (MQ=255)

                                                                                                        cTAATGCAGGATCACTAATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                                >  2:711203/1‑151 (MQ=255)

                                                                                                          aaTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCCGCTGCCGATCGTGGCGGCGt                                              >  8:148562/1‑151 (MQ=255)

                                                                                                           aTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGtt                                             >  8:624791/1‑151 (MQ=255)

                                                                                                            ggCACGAACACGCATCATCTCACGGATATGTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGttt                                            <  3:977984/150‑1 (MQ=255)

                                                                                                             gCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGACGACGGGGGCGGACAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGGTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTCGCGGGGttt                                            >  2:178358/1‑150 (MQ=255)

                                                                                                              cAGGATCTATCATCAGCTGATTTTTATTTGGGGGAGGCGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTa                                           <  8:533612/150‑1 (MQ=255)

                                                                                                               aGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaa                                          >  5:1020657/1‑150 (MQ=255)

                                                                                                                  aTCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACAt                                      >  2:193620/1‑151 (MQ=255)

                                                                                                                        catcatCTGACGGATATTTGGGGGCGGAGGACGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAg                                  <  7:1047507/149‑1 (MQ=255)

                                                                                                                        catcatCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                                >  4:726232/1‑151 (MQ=255)

                                                                                                                         atcatcTGAAGGATTTTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCa                               <  2:353138/151‑1 (MQ=255)

                                                                                                                          tcatcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCATGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTAAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCa                               >  6:783715/1‑150 (MQ=255)

                                                                                                                              ccgaaggattgggggggcAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCaa                          <  1:158624/138‑1 (MQ=255)

                                                                                                                              cTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAATGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCa                           >  1:674208/1‑150 (MQ=255)

                                                                                                                                gAAGGATATGGGGGGGCGGCCGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                            <  7:330011/147‑1 (MQ=255)

                                                                                                                                 aaGGATATTCGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                        <  8:451240/150‑1 (MQ=255)

                                                                                                                                    gctagttGGGGCACGAGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                    <  5:136851/146‑1 (MQ=255)

                                                                                                                                     atatTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                    >  4:87465/1‑150 (MQ=255)

                                                                                                                                          tGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAAt              >  2:92611/1‑151 (MQ=255)

                                                                                                                                          ggggggCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAAt              <  2:433441/150‑1 (MQ=255)

                                                                                                                                              ggaggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAc          >  1:380800/1‑151 (MQ=255)

                                                                                                                                                aggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTATACATCCAGCCAATCAACAGCACAACAATCCAcgc        >  6:327152/1‑151 (MQ=255)

                                                                                                                                                   aggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGa      >  2:518423/1‑150 (MQ=255)

                                                                                                                                                      aCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAAcc  >  2:560063/1‑151 (MQ=255)

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`