Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,122,1240CA28.1% 211.4 / 22.4 148intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (67/25);  new base A (33/3);  total (118/30)
Fisher's exact test for biased strand distribution p-value = 3.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

GCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121974‑4122272
                                                                                                                                                       |                                                                                                                                                    
   ctACCAACAGAGCTATTCCCGGTTGGTGGGTCTGGGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCt                                                                                                                                                     <  5:494297/149‑1 (MQ=255)
     cccccgtgctcttagcccGCGGGGGGGGTCTGTCCATTACAGCAATAGAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTg                                                                                                                                                  <  7:86397/135‑1 (MQ=14)
     aCCAACGGAGCTATTCCCGCTTGGGCGGTCTGTCCCTTACAGCACGTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCtt                                                                                                                                                    <  5:1028687/148‑1 (MQ=255)
            aaGCTATTCCCGCTGGGGTGGTCTGTGCCTTACATACCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACtt                                                                                                                                          <  1:600476/150‑1 (MQ=255)
               cTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAATGTCGAGATCTACCAAG‑TGAGCAATTCAGGCTTGGGAGGTCTTTGATTTGCAGCTCttgt                                                                                                                                        >  2:447464/1‑148 (MQ=17)
               cTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTc                                                                                                                                       >  7:776452/1‑151 (MQ=255)
                tATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTGGGTCGTCTGTGGTGTGCAGGACTTTTc                                                                                                                                       >  3:240097/1‑150 (MQ=255)
                 aTTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTTCTTCGTATGTGATTTGAAGCAATTTTCa                                                                                                                                      >  4:522045/1‑150 (MQ=14)
                 aTTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTGGGACGTATGTGGGGTGAAGGACTTTTCa                                                                                                                                      >  3:842015/1‑150 (MQ=14)
                 aTTCCCGCGGGGGGGGTGTGTCCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCaa                                                                                                                                     <  7:6035/151‑1 (MQ=255)
                  ttCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTGGGTCGTATGTTCTGTGAAGCACTTTTCaa                                                                                                                                     >  4:258105/1‑150 (MQ=17)
                     ccGCTTGGGGGGTCTGCGCCTTACAGCACTTTCAAATTTTGGAGCGGTAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCCACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAttt                                                                                                                                 <  4:1061941/151‑1 (MQ=255)
                      cGCTTGGGTGGTGTGTACCTTAAAGAACTTTAAAATTTTGGCGCGGGAAACGACACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAtttt                                                                                                                                <  7:328372/151‑1 (MQ=17)
                      cGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTTGTTTGCAGGACTTTTCAAAtttt                                                                                                                                >  3:135599/1‑151 (MQ=255)
                       gCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTGGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTg                                                                                                                               >  7:363445/1‑151 (MQ=255)
                        cTTGGGTGGTCTGTGCATTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTAGGTGTTCTGTTATATTAATAACTTTTCAAAtttatg                                                                                                                              >  8:358882/1‑148 (MQ=11)
                            gggggtCTGGACATTAAAGCAATATCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCg                                                                                                                          <  1:924735/148‑1 (MQ=14)
                             gtggtgTGGGCATTTTAGCTCTTTAATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCgg                                                                                                                         <  4:868447/151‑1 (MQ=14)
                              ggctCTGTGCCTTACATATATTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCggg                                                                                                                        <  1:827414/148‑1 (MQ=255)
                               ggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGGAGCACTTTTCAAATTTTGGAGCggg                                                                                                                        >  8:924140/1‑150 (MQ=255)
                               ggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGCGTGTTCTGTTATTTGCAGCACTTTTCTAATTTAGGAGccgt                                                                                                                        >  5:677711/1‑147 (MQ=18)
                                gtCTGTCCATTAAATCACGATCATAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGaa                                                                                                                      <  3:233668/151‑1 (MQ=14)
                                  cTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTGGGTGGTCTGTGATTTTGAGCAATTTTCAAATTTTGGAGCGGGAAAc                                                                                                                    >  3:337234/1‑151 (MQ=255)
                                   tgtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTCGGTCGTCTGTTATTTGAAGAAATTTTCAAATTTTTGAGAGGGaaagc                                                                                                                   >  2:371669/1‑149 (MQ=17)
                                     tgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTGGGAGGTCTGTTATGTGACGGAATTTTCAAATTTTGGAGCGGGAACCg                                                                                                                   >  4:293862/1‑149 (MQ=17)
                                      gCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGTTGGTATGTGATTTTGAGCAATTTTCAAATTTATGAGAGGGAAACgagc                                                                                                                >  8:1011323/1‑150 (MQ=17)
                                        cTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGGAGAACTTTTCAAATTTTGGAGCGGGAACCGAGAcg                                                                                                              >  3:64908/1‑150 (MQ=255)
                                         ttACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATACCCGCTTGCGACGTATATGATAAGTAGTAATTTTCAAATTTTTGAGTCGGAAACGATAcac                                                                                                             >  2:575647/1‑149 (MQ=14)
                                          taccgctccatCACATTTTTCATCGGGAAACGACACTCTAACGCTCGACCACGATCTTGGGATGTGCGTGATAAAAAAAA‑TGAGCAATTACGGCTTGGGTGGTGGGGGATTTTAATTTGTTTTAAAATTTTGGAGCGGGAAACGAGACTc                                                                                                             <  8:429437/140‑1 (MQ=255)
                                          tACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTTCTTCGTATGTTATATTTCGCACTTTTCTAATTTAGGAGAGGTAAACgaggcacc                                                                                                            >  8:985439/1‑146 (MQ=14)
                                           taatttatttataaTTTTGGAGCGGTAAACGAGACTGTAAATAGCGAACCCGACATTGGATTGGTAGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGGCTGTGCTTTTCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCg                                                                                                            <  3:691996/138‑1 (MQ=14)
                                           aCAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGTAGGTCTGTTATTTTGAGGAATTTTCAAATTTTTGAGAGGTAACCGACGCTCg                                                                                                            >  8:465170/1‑150 (MQ=14)
                                            cAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGCGTCGTCTGTGATATGGAGAACTTTTCAAATTTATGAGAGGGAAAGGAAGCTCGa                                                                                                           >  6:133597/1‑150 (MQ=17)
                                                tatataataTTTTGCATAGGGACTCGAGACTCGTACTCGCGACCCATAAATTGTGACGGTCGTGCTATAACAAC‑TTAGATATTCCCTCTGGGGTGGTCTGGTCCTTACATCTC‑TTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTcg                                                                                                      <  4:293184/142‑1 (MQ=2)
                                                aCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGAAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTACGTATTATTTTATATGAAGAACTATTCAAATTTAGGAGAGGTAACCTAAACACGaaata                                                                                                       >  6:743721/1‑147 (MQ=2)
                                                   ttCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTcgcg                                                                                                    >  5:849469/1‑150 (MQ=255)
                                                          tttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGAAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTAGCGAcccctcc                                                                                            >  3:820278/1‑148 (MQ=255)
                                                           ttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACACCGAcc                                                                                           >  1:273560/1‑151 (MQ=255)
                                                              gAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGATGTGAAGCACTTTTCAAATTTTGGAGAGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTg                                                                                        >  7:315068/1‑151 (MQ=255)
                                                               cgCGGGAAACGAGACTCGAACTCGCGCCCCGGACCTTGGCTAGGTCGTGCTCTACCACC‑TTAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                       <  1:447921/150‑1 (MQ=255)
                                                               aGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCATCGGACGTCTGTGATATTAAGCAATTTTCAAATTTATGAGACCTAAACCAAACTCGAAATCACGACCACGACCtagc                                                                                       >  6:335135/1‑148 (MQ=2)
                                                                 cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGAACTCGCGACCCCGACCTAGGc                                                                                      >  2:187221/1‑150 (MQ=255)
                                                                  gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTGGGTGGTCTGTTATTTGGAGCAATTTTCAAATTTTTGAGCGGGAAACGAGCCTGGAACTAGCGACACCGCGCTTGGCa                                                                                     >  1:386388/1‑150 (MQ=14)
                                                                    gAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGGAGGACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAAATCGCGACCCAGCCCTTGGCAAgg                                                                                  >  7:535403/1‑151 (MQ=14)
                                                                     aaaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTGGGTGGTCTGTGATTTGAAGGACTTTTCAAATTTTTGAGCGGGAAAGGAGACGGGAACTCGCGACCCCGACCtgcccaagg                                                                                  >  5:733962/1‑142 (MQ=14)
                                                                          agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGCGGGGGAAACGCGACTCGAACTCGCGACCCCGCCCTTGGCATGGTCCTGc                                                                            >  3:571530/1‑151 (MQ=14)
                                                                             cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAACCGAGACACGAACTCGCGACCCCGACATACGAAAGGTccagcac                                                                          >  6:583821/1‑144 (MQ=14)
                                                                             cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTGGTTGGTCTGTGATTTGGAGAACTTTTCAAATTTTGGAGAGGGAACCGAGACTCGAACTCGCGACCAAGAACTTCGAAAGGTCGTGctct                                                                         >  2:442766/1‑151 (MQ=14)
                                                                              tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGGACTTTTCAAATTTTGGAGGGGGAAACGCGAGTCGAACTCGCGACCACGAGCTTGGCAAGGTCGTGctct                                                                         >  3:320075/1‑150 (MQ=14)
                                                                                ccccccccccccccGCCCGTCGCAAGGGCCTGCTCTACCAACTTTTTTTTTTCCCTTTTTGGGGGAC‑GTGCTTTTCATCAATTTTCTAATTTTGTATCTGGATACTAGACTCGAACCCGATATCCCGACCTTGGCAAGGTCGTGCTCTAcc                                                                      <  4:1020301/141‑1 (MQ=2)
                                                                                  aCTCTCGACCCCGTCCTTGGCAAGGTTTTGCTATACCTAC‑TGTGCTATTCGCGGTTTGGTGGTCTTGGGTTTGAAGGAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCaa                                                                    <  8:363081/151‑1 (MQ=14)
                                                                                  aCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGTTGTTCTGTGCTGTGCAGGACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACACCGCACTTCGCAAGGTCGTGCTCTACCa                                                                     >  6:176186/1‑150 (MQ=14)
                                                                                   cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGATCTCGCGACCCCGCCCTTGGGAAGGTCGTGCTCTACCa                                                                     >  5:170460/1‑149 (MQ=255)
                                                                                     cgcgACCCCGCCCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCGTGGGTGTTCTGTGGTTTGGCGGACTTTTCTAATTTGGGCGGGGGTAACGCGCGTCGATGTCGCGACCCCGCGGTGGGGAAGGTCGGGCTCTACCAACTg                                                                 >  1:99975/1‑151 (MQ=9)
                                                                                       cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGCGTGGTCTGTGATTTTAAGGAATTTTCAAATTTTGGAGAGGTAAACGACACGGGAAATATCGACCCCTAGATTGGCAAGGTCCTCCACTACAAACTGat                                                               >  8:716728/1‑150 (MQ=2)
                                                                                          ccccGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCt                                                             >  6:44094/1‑150 (MQ=38)
                                                                                            ccGCCCTTGGCCACGTCGGGGCGTACCAAC‑TCAGCAATACCCCCTGGGGTGGGGGGGGATATTAATAACTTATAAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAtt                                                          <  3:517994/151‑1 (MQ=2)
                                                                                                ccTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCTAATTTTGGAGCGGGAAACGAGCCTCGATATCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACAAACTGATCTATTCCcg                                                      >  1:782640/1‑151 (MQ=14)
                                                                                                ccTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGGCGTGCTCTACCAAGTGAGCTATTCCcg                                                      >  5:835557/1‑151 (MQ=255)
                                                                                                 cTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTTTGCTTTGGAGAACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCAGCGCTTGGCAAGGTCGTGCTCTAAAAACTGATCTATTCCcgc                                                     >  5:576541/1‑151 (MQ=14)
                                                                                                 cTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGGAGGACTTTTCAAATTTTGGAGAGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAAAAACTGAGCTATTCCcgc                                                     >  1:989901/1‑151 (MQ=14)
                                                                                                    ggCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGAGCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGt                                                   >  5:20562/1‑150 (MQ=255)
                                                                                                     gCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGAAGCACTTTTCAAATTTTGGAGCGGGTAACGAGACTCGAACTCGCGACCCCGACCTTCGCAAGGTCGTGCGCTACAAACTGAGCTATTCCCGCGta                                                  >  4:503667/1‑150 (MQ=255)
                                                                                                      cgctgtgggggtcccccAAC‑CGTGCTATGGCCGGTGGGGTGGGTTGTGATTTTCACTTCTTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGtact                                                <  7:493315/136‑1 (MQ=11)
                                                                                                            ggggCTCTACCACC‑TGAGCGATGCCAGCTTGGGGGGGCGGTGCCGTGCAGCCCTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTaa                                          <  1:216533/148‑1 (MQ=14)
                                                                                                            cGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTAGTACTTa                                           >  7:137920/1‑150 (MQ=255)
                                                                                                             gTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGCACTTTTCAAATTTTTGAGCGGGAAACGCGCCTCGAACTAGCGACCCCGACCTTGGCAAGGTCGTCCTCTACCAACTGAGCTATTCCCTCGTAGTACTTAAt                                         >  1:38882/1‑151 (MQ=14)
                                                                                                             gTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGAGGTCTGTGCTGTGCAGCACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGAAATCGCGACACCGACATTCGCAAGGTCGTGCTCTAAAAACTGAGCTATTCCAGGGTACTACTTAAt                                         >  5:689325/1‑151 (MQ=14)
                                                                                                              tGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTATGTGGTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGCGCCTCGAGCTCGCGACCCCGCGCTTGGCAAGGTCGTGCTCTACCAACTTAGGTATTCCCGCGTACTACTTAAtt                                        >  1:395595/1‑151 (MQ=17)
                                                                                                               gCTCTACCAAC‑TGAGCTATTCCCGCTTGGGAGGTCTGTGATGTGCAGGACTTTTCAAATTTTGGAGAGGGAACCGAGACACGAACTCGCGACACCGAACTAGGGAAGGTCGTGCGCTACCAACTGAGCTATTCCAGAGTAGTACATAAttt                                       >  2:198344/1‑151 (MQ=14)
                                                                                                               gCTCTACCAAC‑TGAGCTATTCCCGCTTCGGAGGTCTGTGATTTGAAGGACTTTTCAAATTTTTGAGCCGGAAACGACACTCCAACTAGCGACCCAGCCGTTCGCAAGGTCCTCCTCTACAAACTGAGCTATTCCCGCGTACAACTTTAttt                                       >  3:543010/1‑151 (MQ=14)
                                                                                                                 tctACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                     >  6:408069/1‑151 (MQ=255)
                                                                                                                 tctACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                     >  7:792165/1‑151 (MQ=255)
                                                                                                                 tctACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                     >  5:977431/1‑151 (MQ=255)
                                                                                                                  ctACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCAGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGc                                    >  8:569984/1‑151 (MQ=255)
                                                                                                                    aCCAAC‑TGAGCTATTCCCGCTTGCGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTAGCGACCCCGAACTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCTGGAACTACTTAATTTTgaat                                  >  8:214783/1‑148 (MQ=14)
                                                                                                                       aaC‑TGATGTTTTCCCGCTTGGGGGGGCGGTGCCGTGCCCGTCTTTTGACTTTGTGGAGGGCGAACGGCGCCTCTAACGCGCTACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCg                               <  7:357592/151‑1 (MQ=12)
                                                                                                                       aaC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCg                               >  7:687997/1‑151 (MQ=255)
                                                                                                                         c‑TGAGCTATTCTCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                              >  3:39629/1‑150 (MQ=255)
                                                                                                                           tGAGCTATTCCCGCTTGGGTGGTCTGTGCGTTACAGCAC‑TTTAAAAATTTGGAGCGGGAAACGAGACTCGAACTAGCGACCCCGACCTTGGCAAGGTACTGCTCTACCAACTTAGCTATTACCGCGTAGGAGTTAAtttatattgagtaat                           >  2:336358/1‑139 (MQ=11)
                                                                                                                                tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                       >  4:797710/1‑151 (MQ=255)
                                                                                                                                tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                       >  2:803551/1‑151 (MQ=255)
                                                                                                                                 aTTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCg                      >  5:338457/1‑151 (MQ=255)
                                                                                                                                 aTTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCg                      >  7:823798/1‑151 (MQ=255)
                                                                                                                                 aTTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCg                      >  2:537035/1‑151 (MQ=255)
                                                                                                                                 aTTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCg                      >  4:24373/1‑151 (MQ=255)
                                                                                                                                   tCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  6:256722/1‑150 (MQ=255)
                                                                                                                                      tgCTTTGGTGGTCTGTGTTTTGATGTAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCa                    <  8:117667/147‑1 (MQ=18)
                                                                                                                                      cGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAttt                 >  7:180537/1‑151 (MQ=255)
                                                                                                                                        cTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCt               >  6:481859/1‑151 (MQ=255)
                                                                                                                                         ttGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTg              >  5:650877/1‑151 (MQ=255)
                                                                                                                                          tGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGc             >  6:1018151/1‑151 (MQ=255)
                                                                                                                                            ggtggggggtgCCTTGCAGCAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgt           <  7:998095/143‑1 (MQ=255)
                                                                                                                                             gtggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtc          >  5:292091/1‑151 (MQ=255)
                                                                                                                                              tggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcg         >  1:13231/1‑151 (MQ=255)
                                                                                                                                                gtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt       >  4:616253/1‑151 (MQ=255)
                                                                                                                                                   tgtgCCTTACAGCAC‑TTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAGCTGAGCTATTCCCGCTTGCTTCGTATTTTTTGTTGCGGAATTTTCAAATTTTTGAGGCGGTACgg   >  4:853661/1‑151 (MQ=11)
                                                                                                                                                    gtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACgg   >  2:946758/1‑151 (MQ=255)
                                                                                                                                                      gCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTTCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  7:1015113/1‑150 (MQ=255)
                                                                                                                                                      gCTTTGCAGCACTCTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTTCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  2:879388/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
GCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAC‑TGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121974‑4122272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑