Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2970AG29.7% 329.4 / 157.7 190intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (120/10);  new base G (48/7);  total (169/21)
Fisher's exact test for biased strand distribution p-value = 2.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-01

Only 100 of 194 total aligned reads displayed.

ATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936178‑936447
                                                                                                                        |                                                                                                                                                      
atcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGCGGAGGGGGTGAATACATCTCAATTTAATTGTTTGCGGGGGCATGATTAGACCctacg                                                                                                                         >  7:1939235/1‑151 (MQ=11)
           ttGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACGTCATCAAATTTTAATTGGtt                                                                                                                                                 >  4:1433976/1‑116 (MQ=255)
                  cGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCAACAAAATTTAATTGGtt                                                                                                                                                 >  8:531136/1‑109 (MQ=255)
                          ggATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCGCGGTAGTGCATCTCTTCAACAAAATTTAATTGGTTGCGGCTGGGTGTTTTGCACAGACCACATTAGGGTTAttatacagaca                                                                                                  >  8:1114092/1‑138 (MQ=2)
                               tGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGCCGACCTTCGGGTTATGAGCCCGACGAGCTAca                                                                                           >  7:801166/1‑149 (MQ=255)
                                           cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTCAGGGGGAAGTTTATGACCAGACGACCTTGGGCTTATTAGGCAGAACAGCTaaagccttatccacac                                                                              >  7:354976/1‑136 (MQ=9)
                                                            gacgaGCTACCAGGCTGCTCCACCCCGCGTCCCCGTACTGCTTTTCTTCATCTAATTTTAATTGGTTGCGGGGGCCGGTTTTGAACCGACGACCTTCGGGTTATGATCCCGACGACCTACCAGCCTGATCCCCCCCGCGTCCGTCGATgcg                                                             >  7:1460913/1‑151 (MQ=14)
                                                              cgaGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATgcgcc                                                           >  7:812995/1‑150 (MQ=255)
                                                                   tACCAGGCTGCTCCACCCCGCGTCACAGTAATGCTTAACTTCATCAAATTTTATTTGGTTGCGGGGGGATGATTTGAACCTAAGAGATTAGGGTTATGTTACAGAAGAGAGAACAGGCTGCTCCACACAGATTCAGTGTATGCTCACtat                                                       >  3:129899/1‑150 (MQ=9)
                                                                     ccAGGCTGCTCCACCCCGCGTCCCCGTAGTGCGTGACTTCAGCAAAGTTTAATTGGTTGCGGGGGCAGGATTTGCACCGACGACATTCGGGTTATGAGGCCGACGAGCGAACAGGCTTCACCCCACAGAGTCGGTGGAttgtccctatacg                                                    >  6:564851/1‑139 (MQ=9)
                                                                       aGGCTGCTCCACCCCGCGTCCCCGTACTGCTTTACTTCATCAAATTTTAATTGCTTGCGGGGGCGGGATTTGAACCGACGACCTTCGGGTTATGAGACCGACGAGCTAAAAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTcc                                                  >  5:801373/1‑151 (MQ=18)
                                                                        ggCTGCTCCACCCCGCGTCACCGTACTCCGTGACTTCATCAAATTTTAATTCGTTGCGCGGGAAGTATTAGAACAGAAGACATTAGGCATATGATACAGACGAACTACCACGCTGCTCCACCCCGAGTCCGTGGATGAGCACTATACtacc                                                 >  2:1237785/1‑148 (MQ=12)
                                                                            gctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTCGTTGCGGGGGCAGGATTTGAACCGACGACCTTCGGGTTAAGAGCCCGACGAGCTACCACGCTCCTCCACACCTCGTCAGTGGATGCTCACTATACTCCGCTcg                                             >  4:361090/1‑151 (MQ=17)
                                                                              tCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                           >  5:6420/1‑151 (MQ=255)
                                                                               ccACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGCTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCGCTCGCGt                                          >  6:1604555/1‑151 (MQ=255)
                                                                                cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                         >  8:485419/1‑151 (MQ=255)
                                                                                cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                         >  7:1909864/1‑151 (MQ=255)
                                                                                cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCAGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                         >  1:88580/1‑151 (MQ=255)
                                                                                 aCCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                        >  4:682614/1‑151 (MQ=255)
                                                                                  ccccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCTCTATACTCCTCTCGCGtttt                                       >  5:132912/1‑151 (MQ=11)
                                                                                   cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  8:2017309/1‑151 (MQ=255)
                                                                                   cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  3:516172/1‑151 (MQ=255)
                                                                                    ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  1:447578/1‑150 (MQ=255)
                                                                                    ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  5:105385/1‑150 (MQ=255)
                                                                                    ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  7:1328439/1‑150 (MQ=255)
                                                                                    ccGCGTCACCGTACTGCTTTAATTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  4:1481750/1‑150 (MQ=255)
                                                                                     ggctgttacGTTCTGCTTTTATTTATTAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGACCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                                    <  8:1347407/143‑1 (MQ=18)
                                                                                      gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                                   >  3:1603990/1‑151 (MQ=255)
                                                                                       cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  1:1061018/1‑151 (MQ=255)
                                                                                       cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  7:499487/1‑151 (MQ=255)
                                                                                         tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGAGGATTCCGAGTATACTCCCCTctg                                            >  5:592946/1‑137 (MQ=14)
                                                                                          cACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACACCACTAGATATTTCATGCAAc                               >  5:280792/1‑151 (MQ=11)
                                                                                          cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                        <  4:1656924/142‑1 (MQ=255)
                                                                                           aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                              >  4:969800/1‑151 (MQ=255)
                                                                                            acGTACTGGGTTACTTCTTCAAATTGTATTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                             <  7:1150697/150‑1 (MQ=255)
                                                                                             cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGTAAGCGAACTATACAACACTAGATTTTTCATTCAACCtt                            >  2:509877/1‑151 (MQ=11)
                                                                                             cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                            >  8:340692/1‑151 (MQ=255)
                                                                                             cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCACGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                            >  7:697092/1‑151 (MQ=255)
                                                                                              gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGAGGAGGCGGTCTATACTACGCTAGAGTTTTCATTCAACCttt                           >  8:1727907/1‑151 (MQ=11)
                                                                                              gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                           >  4:837279/1‑151 (MQ=255)
                                                                                               tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTAGGCCAAATATACTACCCTAGAGATTTCGTGCAACCttat                          >  6:2032153/1‑149 (MQ=11)
                                                                                               tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  1:1463256/1‑151 (MQ=255)
                                                                                                tcggattttATTTCTTCAAATTTGATTGGTTGCGGGGGCGGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          <  6:288046/144‑1 (MQ=18)
                                                                                                aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                         >  1:27139/1‑151 (MQ=255)
                                                                                                aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                         >  6:1401910/1‑151 (MQ=255)
                                                                                                  tgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGCTTTTTCATTCAACCttttttt                       >  5:1180283/5‑151 (MQ=17)
                                                                                                  tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       >  8:216208/1‑151 (MQ=255)
                                                                                                  tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAAACGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       >  3:1824924/1‑151 (MQ=255)
                                                                                                   gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTTATGCAACCtttttttt                      >  7:539176/1‑151 (MQ=255)
                                                                                                    cttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGATCCACCCCGCGTCCGTGGATTCGGAGTATACTGCGATCGAGTTTTCATGCaggcgttatttt                      >  6:1275950/3‑139 (MQ=11)
                                                                                                    cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  2:1506196/1‑150 (MQ=255)
                                                                                                     ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCGGGTTTTTAc                    >  1:1104566/2‑151 (MQ=17)
                                                                                                      tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                    >  7:616687/1‑151 (MQ=255)
                                                                                                      tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCGTTTTTTTAc                    >  2:171291/1‑151 (MQ=255)
                                                                                                       ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc                   >  7:1513589/1‑151 (MQ=255)
                                                                                                       ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc                   >  3:78052/1‑151 (MQ=255)
                                                                                                       ttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCTGGTTTTCATGCACCCTTGTTTTTAccc                  >  5:250545/1‑151 (MQ=17)
                                                                                                        tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  2:667265/1‑151 (MQ=255)
                                                                                                        tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  6:375822/1‑151 (MQ=255)
                                                                                                        tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  4:1288345/1‑151 (MQ=255)
                                                                                                        tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGCACTATACTcccatggcgta                                         >  3:1150120/1‑118 (MQ=14)
                                                                                                        tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCTCTATACTCCGATCGCGTTTTCATGCCCCCGTGTTTTTAcgcc                 >  3:1441885/1‑151 (MQ=14)
                                                                                                        tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGATGCGCACTATACTCCGCTCGAGTTTTCATTCAACCTTAATTTTAaccc                 >  6:1016305/1‑151 (MQ=17)
                                                                                                        cgttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGAGGATGCGCAATATACTACCCTAGCTTTTTCATGCAACCTTAATTTTAacc                  >  4:302666/16‑151 (MQ=11)
                                                                                                         aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACAGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc                 >  1:1429620/1‑151 (MQ=255)
                                                                                                         aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTAAGCGATAAATACTACACTCTATATTTCATGCAGCCTTATTTTTAacccc                >  2:1328466/1‑150 (MQ=11)
                                                                                                         aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCAGAATACTACTCTCGAGTTTTCATGCCACCTTGTTTTTaacacc                >  6:536743/1‑146 (MQ=14)
                                                                                                          cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCCCTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccccc                >  1:1875721/1‑150 (MQ=255)
                                                                                                          cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGAGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  7:281249/1‑149 (MQ=255)
                                                                                                          aattcataaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc                 <  8:581880/143‑1 (MQ=255)
                                                                                                           ttCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGGTCAATACTCCTCTCTCTTTTTGCTTCAGGCGTTATTTTTCCCCGAt              >  5:1366425/1‑151 (MQ=11)
                                                                                                            tcattaAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGa               <  6:132708/150‑1 (MQ=255)
                                                                                                            tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              >  1:996521/1‑151 (MQ=255)
                                                                                                            tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              >  4:195521/1‑151 (MQ=255)
                                                                                                            tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              >  6:764957/1‑151 (MQ=255)
                                                                                                            tcatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCAATATAGATTTCATGCAACCTTTATTTTAACCCGAt              >  4:1492796/1‑150 (MQ=17)
                                                                                                             gtttgttctatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              <  1:1186876/140‑1 (MQ=255)
                                                                                                             catcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGAAGAATACTACACTCTAGTATTCATTCAACCTTATTTTTagcaccatc             >  6:1851632/1‑142 (MQ=14)
                                                                                                                caAATTTTAATTGGTTGCGGGGGCCGGATTTGTACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg          >  1:825435/1‑151 (MQ=255)
                                                                                                                caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg          >  5:1666989/1‑151 (MQ=255)
                                                                                                                caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg          >  7:1281742/1‑151 (MQ=255)
                                                                                                                 gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCTTTTTCATGCAACCtttt                          >  6:1944423/7‑134 (MQ=255)
                                                                                                                 gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          <  5:1945289/128‑1 (MQ=255)
                                                                                                                  aaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAGTATACTCCGCTCGCGTTTTCTTGCAAGCTGTTTTTTACCCCCATCACgg         >  1:1774244/4‑150 (MQ=18)
                                                                                                                  aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  7:424105/1‑150 (MQ=255)
                                                                                                                  aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  7:1166011/1‑150 (MQ=255)
                                                                                                                   aatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGCAATATACTCCGCTAGATTTTTCATGCAACCTTATTTTTAACCCCATCACGCAt       >  2:840992/3‑151 (MQ=17)
                                                                                                                   aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt       >  1:1694418/1‑151 (MQ=255)
                                                                                                                   aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt       >  7:1057169/1‑151 (MQ=255)
                                                                                                                    ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt      >  8:83034/1‑151 (MQ=255)
                                                                                                                    caatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTgg                                                                  <  5:944837/88‑1 (MQ=14)
                                                                                                                    caatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             <  1:576867/141‑1 (MQ=255)
                                                                                                                    atttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGATGCGCACAATACACCGCTCGCGTTTTCATGCAACCTTTTTTTTAACCACATCAcccatt      >  2:610278/2‑146 (MQ=18)
                                                                                                                     tttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAAGCGCACTATACTCCGATATCGTATTCATGCAACCTTTTTTTTAACCCGATCACGCAttt     >  4:1402000/1‑151 (MQ=18)
                                                                                                                     aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                                   <  8:178712/119‑1 (MQ=255)
                                                                                                                     aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTACGCTCGCGTTTTCATg                                   >  7:178740/3‑121 (MQ=255)
                                                                                                                      ttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    >  4:1819766/1‑151 (MQ=255)
                                                                                                                       tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTgg   >  2:1927135/1‑151 (MQ=255)
                                                                                                                       tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTgg   >  3:944749/1‑151 (MQ=255)
                                                                                                                        aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTcc                                                  >  7:581786/1‑103 (MQ=255)
                                                                                                                        |                                                                                                                                                      
ATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936178‑936447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: