Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6050AT24.3% 400.9 / 130.7 247intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (117/67);  new base T (47/12);  total (166/81)
Fisher's exact test for biased strand distribution p-value = 2.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

Only 100 of 264 total aligned reads displayed.

ATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065455‑1065754
                                                                                                                                                        |                                                                                                                                                     
atgaaatcctCTGCAGTCCCGTAATTTGCCCCTGGCATCGGGAGCTCAGCTGGCGAGACGAATCGGCGAGGATGCGTTGGGTCGTAGGTTGTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATca                                                                                                                                                       <  5:801856/142‑1 (MQ=2)
  cAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGCCTTTAGTAGCGGTATC‑‑AATATca                                                                                                                                                       >  5:5523/1‑149 (MQ=255)
     aaaaTCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGt                                                                                                                                                  >  5:1992069/1‑151 (MQ=255)
           tGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAGC‑‑AATATCAGGAGTaaatt                                                                                                                                             >  8:465713/1‑148 (MQ=255)
                taacgtaaTTTCCCCCCTGCATCCGGAGCTCAGCTGGCCATACTAATCGGCTACCAACCGGTGGGTCCCAGCTTCTAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAGAATAAGtt                                                                                                                                        <  3:1999512/150‑1 (MQ=2)
                             ccGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTATATATTCCTGAAGTAGCGGTAGG‑‑AATATCAGCAGTAAAATAAATGTCCCGAtaccta                                                                                                                            >  7:1104861/1‑144 (MQ=17)
                                ttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTa                                                                                                                        <  7:346408/149‑1 (MQ=255)
                                    aTCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTTTACGAGATATTGATGTATTAGGGGTAGC‑‑AATATGTGCAGTAAAATAAATTTCCCGATGCAtatgaagagc                                                                                                                    >  7:508749/1‑141 (MQ=17)
                                      ccGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCTAAATATGCATGAATTAACGGATAC‑‑AATATCAACAGTAATATAAATATCCCgctacctaagaagc                                                                                                                      >  4:648035/1‑133 (MQ=2)
                                              aagctggtttttgTACTCGGCGACGAGCGGTCCGGTCGGCGTGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTATTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATaga                                                                                                         <  8:831904/139‑1 (MQ=14)
                                                    catacacgacgcgccTACGACCCGAGCGGGCGGAGCTTCCAATCCTCCCGGATTCAACATCTCTTACTTGTTATCGCTTTTGTCGCGGGAGT‑‑AATCTGACGACTTAAATTCTTATCACGATGCATCCGTAGCTCAGCTGGATAGAGTACTc                                                                                                   <  7:385819/137‑1 (MQ=9)
                                                      tAGATTACTCGGTTACGATCGCTTCGGTCTGAGCTTCTAATCCTCCCTGATGCACCATCTCTTACTTTATATGGCTTTAGTAGCGTTATC‑‑AATATCAGCAGTAAATTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACt                                                                                                    <  4:1696121/148‑1 (MQ=14)
                                                        aacgacgcgacTACGAAACGAGCGGTGTGAGGTGAGAAGACTCGTGGTCTCACCATCTCTTACTTGATCGGGCTTTACTAGCGCTATA‑‑AATATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCt                                                                                               <  1:1223178/141‑1 (MQ=9)
                                                         tctACTCGGCGACGAGCGCTGCGGTCGTCGGGTCTAATCCTCCCTGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCt                                                                                               <  1:259731/148‑1 (MQ=14)
                                                            aCTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCAGTAGCACATCTGTATAGAGTACTCGGCTACg                                                                                            >  7:749799/1‑150 (MQ=255)
                                                            aCTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGCTGCAGCCGTAGCGCAGCGGGATTGAGTACTCGGCTTCg                                                                                            >  1:1025177/1‑150 (MQ=21)
                                                            aCTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTTGATATTGCTTTATTAGCGGTTTC‑‑AATATCTGGAGTAAATTAAATTTCGCGATACATAAGTAGAACATCTTTTTAGAGTACTCGTCTACg                                                                                            >  8:383575/1‑150 (MQ=18)
                                                               cGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTGGTACTTGATATGGCTGTATGAGCGGTTGG‑‑AATATCAGCAGTAATATAAATTTCCCGATGCCTCCGTAGCACAGCTCGAGAGACTACTCGGCTACGAAc                                                                                         >  7:1570997/1‑150 (MQ=25)
                                                                 gcgacgagcgcgcgggTCGGCGCGTCTAATCCTCCGTGTTTCACCATCTCTTACTTTATATGGCTTTATTAGCTGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGa                                                                                      <  6:884503/137‑1 (MQ=14)
                                                                    aCGATCGGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCg                                                                                   <  7:70457/151‑1 (MQ=255)
                                                                     cgatcgctccgctCGGCGGGTCTAATCCTCCCGGATTCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCgg                                                                                  <  5:1782397/139‑1 (MQ=14)
                                                                              cgctcggcgcgtctaaTGCTCCCTGATGCACCATCTATTACTTTATATGGCTTTACTAGCTGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGtt                                                                         <  3:1885864/137‑1 (MQ=14)
                                                                              cGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTAGATATTGCGATATTAACGGTAAC‑‑AATATCAACAGTAAAATAAATTTCCCGATCCATAAGTAGCTAAGCTGGAGATATTACTCAGGTAAGGAGGGAGCTGTCGTAGGtt                                                                         >  8:1554066/1‑151 (MQ=16)
                                                                               gctcggcgcgtctaaTCCTCCCGGATTCACCATCTATTACTTTATATGGCTTTAGTAGCTCTATA‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                        <  1:141452/138‑1 (MQ=14)
                                                                                gTCGTAGGTTCTAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAgaataagt                                                                                                                                         <  5:383257/85‑3 (MQ=11)
                                                                                gTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTTGATATGCCTGTAGTAACGGATGC‑‑AAGATCTAGAGTAAAATAAATTTCCCGCTGCGTCCGTAGCTCAGCTGGATAGAGAACTCGGCTTCGAGCGGAGCGGTCGGAGGTTct                                                                       >  2:663455/1‑150 (MQ=17)
                                                                                 tCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATTGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCGGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCTAGCGGTCGGAGGTTCg                                                                       >  4:2026876/1‑150 (MQ=255)
                                                                                 tCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATcagcag                                                                                                                                                   >  7:1651726/1‑74 (MQ=255)
                                                                                  cGGAGGTTCGAATCCTCCCGGATGCACCATATTTTACTTGATATTGATTTATTAGCGGTTTG‑‑AATATCTGCAGTAAATTAAATTTCCCGATTCGTAGGTATCTCTTCTGTATACGGTAGTATGCTAAGAACCGTGAGGTCGTAGGTTCGat                                                                     >  3:782239/1‑150 (MQ=255)
                                                                                     cgagtctaagcctccCGTCCGCACCATCTCTTACTTTATATCGTTTTACGAGCGCTATA‑‑AATATCAGCAGTAAACTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcc                                                                  <  5:1650793/141‑1 (MQ=14)
                                                                                          cGAAGCCTCCCGGATGCACCATCTCTTACTTGATATGTGTTTAGTAGCCGTATC‑‑AATAGCAGCACTAAAATAAATATCCCTCTGCCTCCGTAAGTCAGCTGCAGAGAGTAGGCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTccc                                                              <  5:631790/150‑1 (MQ=14)
                                                                                           gAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                            >  7:1800970/1‑151 (MQ=40)
                                                                                           gAATCCTCCCGGACGCACCATCTCTTACTTTATAGGGCTTTAGTAGCGCTATC‑‑AATATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                            <  3:323899/151‑1 (MQ=255)
                                                                                            aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                            >  7:253732/1‑150 (MQ=40)
                                                                                            aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAACCGTATA‑‑ACAAGCCCCGGGTAAATTAAATTCACCCTTCATTCTTAGCTAAGCTGGCTAGAGTAATATGATACGAACCGCGCTGGCGGCGGTTGGagtgggcccggc                                                           >  7:1931981/1‑140 (MQ=2)
                                                                                            aaTCCTCCCGGATGCACCATCTATTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATGAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCACAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCATCCCgg                                                            >  2:1472401/1‑150 (MQ=255)
                                                                                                   ccGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAtaataag                                                                                                                                          >  6:1627722/1‑64 (MQ=12)
                                                                                                   ccGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGAGGTATC‑‑AATATAAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACAGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAcc                                                     >  7:85846/1‑150 (MQ=255)
                                                                                                   ccGGAATCACCATCTCTGACGTGAGAGGGGTTTACTAGCGTTATC‑‑AATATCCCCAGTAACCTAAAAATCCCCATGCAGCCGTAGCTCAGCTGGCTAGACTACTCGGCGACGAACCGAGCGGTCCGAGGTTCGAATCCTCCCGGATGCACCa                                                    <  1:354603/151‑1 (MQ=9)
                                                                                                         gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                              >  4:837612/1‑151 (MQ=39)
                                                                                                         ccccgaTCTCTTACGTGATCTGGTTTTACTAGCTGTATA‑‑AATATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                              <  7:942000/146‑1 (MQ=11)
                                                                                                          cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTa                                                                                                                                                 <  3:1700646/51‑1 (MQ=35)
                                                                                                          cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCACGATGCATGAGTAGCTCAGCTGGATAGAGTACTAGACTACGAACCGAGCGGTCGGAGGGTCGACTCCTCCCGGATGCACCATATtctc                                             >  4:1614541/1‑151 (MQ=14)
                                                                                                          cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAAAAAATTACACCAACCATCAGTACAACAGGTGCATATAGTAATCGGCTACGAACCGATCGGTCGGATCAAAGAATCCTCCCGGATGCGCCATATtgtc                                             >  7:1831018/1‑151 (MQ=9)
                                                                                                            ccATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCg                                           >  6:406136/1‑151 (MQ=39)
                                                                                                                 tctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCATGGGAATAGAAGACCGGATGCATCCGTACCGCACGAGTATAGACTAATCGCCTAGGCTCCGAGAACTAAGAGGTTCGAGTCCTGCGGGCTGCGCCatcgtctgggacctg                                      >  3:1408250/1‑138 (MQ=2)
                                                                                                                  ctTAGTTGTTATGTGTTTATTACCGTTTTT‑‑AATATCACGAGTATATTAATATCTCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGaaa                                        <  8:1628392/148‑1 (MQ=11)
                                                                                                                  ctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGaaaac                                      >  6:1061167/1‑148 (MQ=255)
                                                                                                                     aCTTGCTATCGCTGTACTAGCTCTATC‑‑AATATCACCAGTATAATAAAATACGAGCTGCTTCCGATCTTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCa                                  <  4:954599/151‑1 (MQ=14)
                                                                                                                     aCTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc                                   >  6:564023/1‑150 (MQ=255)
                                                                                                                      cTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCTGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc                                   >  3:1216483/1‑149 (MQ=255)
                                                                                                                      cTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTAGGCTACGAACCGAGCGGTCGGAGGTTCGAATACTCCCGGATGCACCATATTATCCgtagaaagga                                  >  5:1383739/1‑141 (MQ=11)
                                                                                                                       ttGATACGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGAGATAttgc                                  >  2:1697222/1‑149 (MQ=14)
                                                                                                                        tGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGc                                >  1:455340/1‑150 (MQ=255)
                                                                                                                                cTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                       >  5:1483379/1‑151 (MQ=255)
                                                                                                                                 tttAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGCAATGAAgg                        >  3:433605/1‑151 (MQ=11)
                                                                                                                                 tttAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCTAAATATTCAGAAATTAAgg                        >  8:250883/1‑151 (MQ=14)
                                                                                                                                 tttAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTACaacggaacagcg                       >  8:1372111/1‑139 (MQ=11)
                                                                                                                                 tttAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                       >  7:1397338/1‑150 (MQ=255)
                                                                                                                                 tttAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCAGAAATCTTCAGAAATGAAGGt                       >  4:1943159/1‑150 (MQ=255)
                                                                                                                                   tagtagCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCATAAATGaacgat                      >  2:1547633/1‑147 (MQ=11)
                                                                                                                                   tagtagCGGTATCAAAAAATATGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATATTCATCAATTAAggat                      >  4:23811/1‑149 (MQ=11)
                                                                                                                                   tagtagCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATc                    >  3:1488726/1‑151 (MQ=255)
                                                                                                                                   tagtagCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATc                    >  3:1120240/1‑151 (MQ=255)
                                                                                                                                    agtagCGTTATC‑‑AATATAAGCATTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTa                      <  2:1964246/148‑1 (MQ=255)
                                                                                                                                    agtagCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTACCGGATGCACCATATTCTACGTTATATTCAGATATTAAgggtt                     >  5:987917/1‑148 (MQ=14)
                                                                                                                                    agtagCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                     >  6:471767/1‑149 (MQ=255)
                                                                                                                                     gtagCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCATCAATGAACGTATc                    >  2:1259928/1‑151 (MQ=1)
                                                                                                                                      tagaggtataaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATc                    <  4:433541/135‑1 (MQ=11)
                                                                                                                                      tagCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGACATCTGGAGCAATGAAgggtt                     >  3:178396/1‑146 (MQ=11)
                                                                                                                                      tagCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                  >  1:1332838/1‑150 (MQ=255)
                                                                                                                                       cggtttata‑‑AATATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                <  7:1544401/146‑1 (MQ=11)
                                                                                                                                       agCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATTcctaaattatcgtat                     >  2:1108249/1‑134 (MQ=14)
                                                                                                                                       agCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCGAAATATTCAGAAATGAACGTAACg                   >  4:1322662/1‑150 (MQ=11)
                                                                                                                                       agCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGAATCCGTAGCTCAGCTTGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCTGATGCACCATATTATACTAAATATTCATATATGATGGTAttc                   >  6:1768614/1‑148 (MQ=14)
                                                                                                                                       agCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCTATGAAGGTATCGa                  >  2:969537/1‑149 (MQ=255)
                                                                                                                                       agCGGTATA‑‑AATATAAGAATTAAAATATATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCgg                                                                                                 <  8:1846110/70‑1 (MQ=17)
                                                                                                                                        gCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                  >  7:1914236/1‑148 (MQ=255)
                                                                                                                                        gCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                  >  5:274995/1‑148 (MQ=255)
                                                                                                                                        gCGGTATC‑‑AATATCAGCAGTAAAATAAAATTGCCGATGCGTCCGTAGATCAGCTAGATAGAGTACTCGGCTACGCAACTAGCGGTCGGAGGTTAGAATAATAACTGATGCACCATATTCTCCGAGATCTTGAGCTATGAAGGTAGGGAagg               >  8:1468425/1‑150 (MQ=9)
                                                                                                                                         cggtatcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCTAAATATTCATGAATGAACGTATGGaat                >  8:168579/13‑150 (MQ=11)
                                                                                                                                          ggtttaataaTATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                <  8:662239/142‑1 (MQ=11)
                                                                                                                                           gTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt            >  8:1136975/1‑151 (MQ=255)
                                                                                                                                            tatcaaaaaatCTGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATc                                      <  5:1713512/117‑1 (MQ=11)
                                                                                                                                             atcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTCTCCGACATATTGCGCTAGCAACGTAAAGAAGAGGt            >  3:1150636/9‑151 (MQ=14)
                                                                                                                                              tC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTg                                                                                                               >  1:1935244/1‑49 (MQ=255)
                                                                                                                                              ac‑‑AAAATCAGCACTAAAGTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTAGTCGGCTACTAAGCTCGCGGGCGGTGGTGCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGc         <  1:1999146/150‑1 (MQ=14)
                                                                                                                                               caaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGATATCTTCAGGTATGATGGTATAGAAGATGTac          >  3:27167/7‑150 (MQ=11)
                                                                                                                                               aaaaaTATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCTGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTa           <  2:37659/147‑1 (MQ=255)
                                                                                                                                                taaaTATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTg                                                                                                               <  2:1934979/47‑1 (MQ=255)
                                                                                                                                                atataatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAg          <  1:1150489/145‑1 (MQ=255)
                                                                                                                                                  aaTATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATATAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg       >  1:1613033/1‑151 (MQ=255)
                                                                                                                                                  aaTATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg       >  5:1117743/1‑151 (MQ=255)
                                                                                                                                                   aaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                           <  6:299804/128‑1 (MQ=255)
                                                                                                                                                   aaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTAAATATTCATAAAGGAAGGGTTGGAAGAGGtggagg       >  8:1981576/3‑145 (MQ=11)
                                                                                                                                                    aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATCTTCATCAATGAACGTATAGAATATGTacagat      >  2:1149594/2‑145 (MQ=11)
                                                                                                                                                     atCAGCACTAAACTACAGTTCCCGCGCCATCCGTAGCTCTGCGGGCTCGAGTCCTCGGCGACGAACCGAGCTGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAt    <  1:663578/151‑1 (MQ=14)
                                                                                                                                                       cagcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt   >  1:149807/1‑150 (MQ=255)
                                                                                                                                                        agcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCgg                                                                                  >  4:688964/1‑70 (MQ=40)
                                                                                                                                                        agcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTa  >  4:1001784/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
ATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065455‑1065754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: