Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6140AG21.7% 438.4 / 134.9 274intergenic (+49/‑14)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (124/56);  new base G (42/8);  total (175/99)
Fisher's exact test for biased strand distribution p-value = 4.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-01

Only 100 of 301 total aligned reads displayed.

GTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCA  >  DH1/1065470‑1065764
                                                                                                                                                   |                                                                                                                                                      
gtaaagtaaGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAATATAAAtt                                                                                                                                                  >  4:677945/1‑151 (MQ=255)
 taacgtaaTTTCCCCCCTGCATCCGGAGCTCAGCTGGCCATACTAATCGGCTACCAAC‑CGGTGGGTCCCAGCTTCTAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAGAATAAGtt                                                                                                                                                  <  3:1999512/150‑1 (MQ=2)
          tttCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTATTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAAGATCAaaataaagtaggctat                                                                                                                                           >  8:323849/1‑139 (MQ=18)
          ccgccatttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATg                                                                                                                                          <  3:235187/141‑1 (MQ=255)
           tcagacgtgtcctcGTAGGTTATCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCa                                                                                                                                        <  7:254886/137‑1 (MQ=25)
              cccactcacccGGAGCTCACCTGGAGAGACGACTCGGCCACGACCGCGAG‑GCTCCCCGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCg                                                                                                                                    <  7:69419/142‑1 (MQ=9)
                 ttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTa                                                                                                                                  <  7:346408/149‑1 (MQ=255)
                  tGCATCCGTAGCTCAGCTGGATAGAGTACTCAGATACGAAC‑AGCGACGTCGAAGGAACGAATCGTAACGGATGGAGAAAAACATAGTTGATATGGATAAATTAAAAAAATA‑‑AATATCAGCAGTAAAATAAATTTCGCGATGCATCCGTAgg                                                                                                                                >  8:385715/1‑150 (MQ=9)
                   gCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCATAATAGATATGCCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAaaataactattcccca                                                                                                                                           >  6:490135/1‑129 (MQ=18)
                     aTCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTTTACGAGATATTGATGTATTAGGGGTAGC‑‑AATATGTGCAGTAAAATAAATTTCCCGATGCAtatgaagagc                                                                                                                              >  7:508749/1‑141 (MQ=17)
                         gTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTTGATATTGATATATTAAGGTTTGC‑‑AATATCACGAGTAATATAAATTTGAAGATGCATCGGTAGCTCAGCTg                                                                                                                         >  8:1475837/1‑151 (MQ=14)
                               cAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTGTAGTAGCGGTATC‑‑AATATCAGCAGTAAATTAAATTTCCCGATGCGTCGGTAGCTctgggggat                                                                                                                      >  4:330335/1‑140 (MQ=25)
                               aagctggtttttgTACTCGGCGACGAGC‑GGTCCGGTCGGCGTGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTATTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATaga                                                                                                                   <  8:831904/139‑1 (MQ=14)
                                aGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTACTACTAAATATGGATGTATTAACGTTAGG‑‑AATATCAACAGTAAATAAAATAACACAAAGCAACAGAAGCTAAGCTGGATag                                                                                                                    >  8:1660957/1‑149 (MQ=9)
                                         aacgacgcgacTACGAAA‑CGAGCGGTGTGAGGTGAGAAGACTCGTGGTCTCACCATCTCTTACTTGATCGGGCTTTACTAGCGCTATA‑‑AATATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCt                                                                                                         <  1:1223178/141‑1 (MQ=9)
                                             aCTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCAGTAGCACATCTGTATAGAGTACTCGGCTACg                                                                                                      >  7:749799/1‑150 (MQ=255)
                                             aCTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGCTGCAGCCGTAGCGCAGCGGGATTGAGTACTCGGCTTCg                                                                                                      >  1:1025177/1‑150 (MQ=21)
                                              cTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACGAGATCTGGCGCTAGGATCGGATGC‑‑AATATGTACAGTAAATTACAGGTCCCGATGCGTACGAAGCGCAGATGCATAGAGTCCTCGGCtgacac                                                                                                    >  7:1642500/1‑146 (MQ=9)
                                                cGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTGGTACTTGATATGGCTGTATGAGCGGTTGG‑‑AATATCAGCAGTAATATAAATTTCCCGATGCCTCCGTAGCACAGCTCGAGAGACTACTCGGCTACGAAc                                                                                                   >  7:1570997/1‑150 (MQ=25)
                                                   gcgacgagcgcgcgggTCGGCGCGTCTAATCCTCCGTGTTTCACCATCTCTTACTTTATATGGCTTTATTAGCTGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGa                                                                                                <  6:884503/137‑1 (MQ=14)
                                                    tACGAAC‑CGAGCGGTCGGAGGTTCGAATACTCCCGGATGCACCATCTGGTACTTGATATGGCGGTAGTAGCGGTAGC‑‑AATATCAGCAGGAAAATAAATGTCCCCCTGCATCGGTAGCGCAGCGGGATAGAGGCCTCCGCTACGACCCGAGc                                                                                              >  5:1741830/1‑151 (MQ=25)
                                                       cgatcgctccgctCGGCGGGTCTAATCCTCCCGGATTCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCgg                                                                                            <  5:1782397/139‑1 (MQ=14)
                                                        aaC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATATTACTAGATATGGCTATAGTAACGTTATC‑‑AATATCAACAGTAAAATAAATTTCCCGATGCATCAATAGATAAGCTGGATAGAGTACTCTGCTACGAACAGAGCGGTc                                                                                          >  4:1014130/1‑151 (MQ=25)
                                                                cgctcggcgcgtctaaTGCTCCCTGATGCACCATCTATTACTTTATATGGCTTTACTAGCTGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGtt                                                                                   <  3:1885864/137‑1 (MQ=14)
                                                                cGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTAGATATTGCGATATTAACGGTAAC‑‑AATATCAACAGTAAAATAAATTTCCCGATCCATAAGTAGCTAAGCTGGAGATATTACTCAGGTAAGGAGGGAGCTGTCGTAGGtt                                                                                   >  8:1554066/1‑151 (MQ=16)
                                                                 gctctgcgattataaTCCTCCGTGTTGCAAGATCTCTTACTTGATTTGGCTTTACTAGCTTTATC‑‑AATATCAGCAGTAAAATAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                                  <  6:1897933/138‑1 (MQ=11)
                                                                   tCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATTGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCGGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCTAGCGGTCGGAGGTTCg                                                                                 >  4:2026876/1‑150 (MQ=255)
                                                                       aGGTTCGAATCCTCCCGGATGCACCATCTGTTACTTGATATTGCGGTAGTAGCGGTTGC‑‑AATATCAGCAGTAAATTAAATTTCCCGATGCATAAGTAGATAAGCTGGATAGAGTACACGGCTACTAACCGAGCGGTCGGAGGTTCGAAtcc                                                                            >  8:160172/1‑151 (MQ=14)
                                                                          ttCGAATCCTCCCGGATGCACCATATGGTACTAGATATGCATATAGTAGCGGTAGC‑‑AATATCAGCAGTAAATTAAATTTCCCGATGCATCAGTAGAACATCTGGATAGAGAACTCGGATACGAACCGAGCGGGCGGAGGTTCGAAtactcc                                                                         >  2:121870/1‑151 (MQ=14)
                                                                            cGAAGCCTCCCGGATGCACCATCTCTTACTTGATATGTGTTTAGTAGCCGTATC‑‑AATAGCAGCACTAAAATAAATATCCCTCTGCCTCCGTAAGTCAGCTGCAGAGAGTAGGCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTccc                                                                        <  5:631790/150‑1 (MQ=14)
                                                                             gAATCCTCCCGGACGCACCATCTCTTACTTTATAGGGCTTTAGTAGCGCTATC‑‑AATATCAGCAGTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                                      <  3:323899/151‑1 (MQ=255)
                                                                              aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAACCGTATA‑‑ACAAGCCCCGGGTAAATTAAATTCACCCTTCATTCTTAGCTAAGCTGGCTAGAGTAATATGATACGAACCGCGCTGGCGGCGGTTGGagtgggcccggc                                                                     >  7:1931981/1‑140 (MQ=2)
                                                                                ccctccCGGACGCACCATCTCTTACTTGATATGGCTTTAGTATATTTATT‑‑AATATAAGGAGTAAAATAAATTTCTCGATTCATCATTAGATCATTTGGATAGAGTACTCTGTTACGATCTGAGCGGTCGGAGGTTCGAATCCTCCCGGATg                                                                   <  8:1857094/150‑1 (MQ=9)
                                                                                    cccGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAcc                                                               >  5:1669903/1‑151 (MQ=39)
                                                                                     ccGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGAGGTATC‑‑AATATAAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACAGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAcc                                                               >  7:85846/1‑150 (MQ=255)
                                                                                            cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAAAAAATTACACCAACCATCAGTACAACAGGTGCATATAGTAATCGGCTACGAACCGATCGGTCGGATCAAAGAATCCTCCCGGATGCGCCATATtgtc                                                       >  7:1831018/1‑151 (MQ=9)
                                                                                              ccATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCg                                                     >  6:406136/1‑151 (MQ=39)
                                                                                                 tctcTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGaa                                                   >  5:1862497/1‑150 (MQ=255)
                                                                                                  ctctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAt                                                 >  1:513080/1‑151 (MQ=255)
                                                                                                   tctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCATGGGAATAGAAGACCGGATGCATCCGTACCGCACGAGTATAGACTAATCGCCTAGGCTCCGAGAACTAAGAGGTTCGAGTCCTGCGGGCTGCGCCatcgtctgggacctg                                                >  3:1408250/1‑138 (MQ=2)
                                                                                                    ctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCt                                               >  3:1779862/1‑151 (MQ=255)
                                                                                                        tttGATATGGCTTTAGTAGCGGTTTA‑‑AATATCAGCAGTTAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCgt                                                    <  4:1910924/141‑2 (MQ=11)
                                                                                                        cTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCTGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc                                             >  3:1216483/1‑149 (MQ=255)
                                                                                                        cTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTAGGCTACGAACCGAGCGGTCGGAGGTTCGAATACTCCCGGATGCACCATATTATCCgtagaaagga                                            >  5:1383739/1‑141 (MQ=11)
                                                                                                         ttGATACGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGAGATAttgc                                            >  2:1697222/1‑149 (MQ=14)
                                                                                                          tGATACGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTCCTAAATCtggct                                           >  1:37759/1‑147 (MQ=14)
                                                                                                            ataggtttttAGTAGGTTTATA‑‑TATATCAGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAAt                                       <  2:777940/144‑1 (MQ=11)
                                                                                                            atacGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACTAAATATTCatc                                          >  2:265669/1‑148 (MQ=14)
                                                                                                             tatGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATg                                      >  6:1118187/1‑151 (MQ=255)
                                                                                                                 gCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAgg                                  >  4:946219/1‑151 (MQ=255)
                                                                                                                 gCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTAAGCAATGAAgg                                  >  4:62433/1‑151 (MQ=255)
                                                                                                                  gtatactagctctata‑‑AATATCAGCAGTAAACTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                                 <  3:1677718/139‑1 (MQ=14)
                                                                                                                   tttAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGCAATGAAgg                                  >  3:433605/1‑151 (MQ=11)
                                                                                                                   tttAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCTAAATATTCAGAAATTAAgg                                  >  8:250883/1‑151 (MQ=14)
                                                                                                                   tttAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCAGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCACGGATGCACCATATAATACTAAATATTCataaattaa                                    >  2:536002/1‑141 (MQ=14)
                                                                                                                   tttAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTACaacggaacagcg                                 >  8:1372111/1‑139 (MQ=11)
                                                                                                                      agtagCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGCGAGGTCGGAGGTTCGAATCCGGCCGGATGCACCATATTCTGCGAAGTCTTCAGCAATGAGGGTGTCg                             >  6:582337/1‑151 (MQ=14)
                                                                                                                      agtagCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCg                             >  3:1138922/1‑151 (MQ=255)
                                                                                                                      agtagCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTAAGAAATCTTGAGCAATGAAGGTATCg                             >  4:1630534/1‑151 (MQ=255)
                                                                                                                      agtagCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATCTTCAGCAATGAAGGTATCg                             >  5:1541215/1‑151 (MQ=255)
                                                                                                                         agCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATTcctaaattatcgtat                               >  2:1108249/1‑134 (MQ=14)
                                                                                                                          gctatata‑‑AAAATCAGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAaga                         <  1:265456/147‑1 (MQ=255)
                                                                                                                          gCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                            >  5:48093/1‑148 (MQ=255)
                                                                                                                          gCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                            >  7:1914236/1‑148 (MQ=255)
                                                                                                                            ggtttaataaTATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                          <  8:662239/142‑1 (MQ=11)
                                                                                                                            ggtatcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCGCCATATTCTCCGAAATATTCAGCAATGAACGTATAGa                            >  1:1495003/12‑148 (MQ=11)
                                                                                                                             ggataaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagag                        <  1:830864/141‑1 (MQ=11)
                                                                                                                             gTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTATCGAagagat                      <  7:1784171/151‑3 (MQ=14)
                                                                                                                             gTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt                      >  8:1136975/1‑151 (MQ=255)
                                                                                                                              tatcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGAAATATTCCTGAAGTAAGGTATGGAAGAgg                       >  1:1357475/10‑151 (MQ=11)
                                                                                                                               cac‑‑AATATCATCACTAAAAAAACTTGCCAGATGCATACGAAGCTCCGCTGGATATACTACTCGTCCTCTAACCTTGCGGTCCGAGGGACTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTa                     <  7:889963/148‑1 (MQ=9)
                                                                                                                                tC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTg                                                                                                                         >  1:1935244/1‑49 (MQ=255)
                                                                                                                                ac‑‑AAAATCAGCACTAAAGTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTAGTCGGCTACTAAGCTCGCGGGCGGTGGTGCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGc                   <  1:1999146/150‑1 (MQ=14)
                                                                                                                                  taaaTATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTg                                                                                                                         <  2:1934979/47‑1 (MQ=255)
                                                                                                                                  atataatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAg                    <  1:1150489/145‑1 (MQ=255)
                                                                                                                                    aaTATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATATAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                 >  1:1613033/1‑151 (MQ=255)
                                                                                                                                    aaTATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                 >  5:1117743/1‑151 (MQ=255)
                                                                                                                                     aaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                                     <  6:299804/128‑1 (MQ=255)
                                                                                                                                     aaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                                     >  5:300214/3‑130 (MQ=255)
                                                                                                                                      aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGa                >  5:816951/2‑150 (MQ=255)
                                                                                                                                      aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATCTTCATCAATGAACGTATAGAATATGTacagat                >  2:1149594/2‑145 (MQ=11)
                                                                                                                                       atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAt              <  3:1015092/151‑1 (MQ=255)
                                                                                                                                       atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAt              >  4:722070/1‑151 (MQ=255)
                                                                                                                                         cagcagTTAATTAAATTTCCCGATGCATCGGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTa            <  7:1476681/151‑1 (MQ=255)
                                                                                                                                           gcatTAAATTAATTTTCCCGATGCATCCGTAGCTGATGTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCGCGGATGCACGATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt             <  8:1765811/148‑1 (MQ=14)
                                                                                                                                           gcatTAAAATAAATTTCCCGATGCATCCGTAGCTCa                                                                                                                             <  2:1430448/36‑1 (MQ=255)
                                                                                                                                           gcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                                  <  4:20389/79‑1 (MQ=38)
                                                                                                                                            cagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTCCGAAATATTCAGAAATGAACGTTTAGAATATCTAGAGTAATTAAc          >  4:1326607/1‑150 (MQ=11)
                                                                                                                                            cagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAAcc         >  4:988532/1‑151 (MQ=255)
                                                                                                                                             agTAAAATAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCg        <  7:110787/151‑1 (MQ=255)
                                                                                                                                             agTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAAccc        >  3:708684/1‑150 (MQ=255)
                                                                                                                                              gTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc       >  2:1576179/1‑151 (MQ=255)
                                                                                                                                               tAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTAc                                                                                                       <  6:1009920/54‑1 (MQ=40)
                                                                                                                                                aaagTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAg     <  2:1494447/151‑1 (MQ=255)
                                                                                                                                                aaagTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGAAATCTTCAGGAATGAGGGTTTGGAAGAGGTAGAGGAATTAACCGc       >  7:221219/1‑149 (MQ=14)
                                                                                                                                                aaagTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACGAGATCTGCATCAAGTAGGGTAAGCAAGAGGTAGCGGAATTaaaataac     >  6:1375461/1‑145 (MQ=11)
                                                                                                                                                aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCgg                                                                                            <  6:1690022/64‑1 (MQ=39)
                                                                                                                                                  aataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGc   >  2:1973123/1‑151 (MQ=255)
                                                                                                                                                   ataactTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCa  <  7:1334392/151‑1 (MQ=255)
                                                                                                                                                   ataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGc   >  3:807101/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAC‑CGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCA  >  DH1/1065470‑1065764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑