Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,326,2870GT24.9% 1031.5 / 97.1 511intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
Reads supporting (aligned to +/- strand):  ref base G (249/120);  new base T (26/96);  total (281/230)
Fisher's exact test for biased strand distribution p-value = 3.69e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 520 total aligned reads displayed.

GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326137‑2326437
                                                                                                                                                      |                                                                                                                                                      
gATGCGCTACGCTTATCAGGCCTAGACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTg                                                                                                                                                        <  8:469949/151‑1 (MQ=17)
gATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTg                                                                                                                                                        <  8:2049575/151‑1 (MQ=32)
  tGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAACAAAACCTACTAGGTATACAATATCAAAATTACGCCTTTTAAAGAGACATTTGa                                                                                                                                                       >  8:311440/1‑150 (MQ=11)
       tACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGAAGAGTTTTACAGCGCCATTTTAAACtg                                                                                                                                                  >  5:1057022/1‑150 (MQ=255)
        aCGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtga                                                                                                                                                 <  8:1743738/150‑1 (MQ=38)
        aCGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTATCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACtta                                                                                                                                                 >  8:1599352/1‑148 (MQ=12)
         cGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAACGCGTTCACGCCGCATCCGGCATGAACAAAGCCTAGGTAGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAActgctg                                                                                                                                               >  3:267311/1‑151 (MQ=255)
          gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCAATTTAAAAtgatc                                                                                                                                               >  2:527648/1‑149 (MQ=16)
             tATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGAc                                                                                                                                             <  8:791948/149‑1 (MQ=40)
              aTCAGGCCTACACGTTCTCTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACaa                                                                                                                                           <  6:220847/150‑1 (MQ=255)
                cAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAAc                                                                                                                                         <  1:1870104/150‑1 (MQ=255)
                 aGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCAGCATACAGCCTTAACGCCCGGAACTCCAGTAACAAATCATAAGGTGTGTAGAATAAGAAAATGACAAAATATACAGCGCCATTTGCGACtgatgccgcccgc                                                                                                                                       >  5:1019372/1‑143 (MQ=2)
                  ggCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCa                                                                                                                                      <  5:272447/151‑1 (MQ=255)
                      tACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACAt                                                                                                                                  >  3:918324/1‑151 (MQ=255)
                       acacGTTCACTGCAATCTTTTGGATTTGAAGGGTTTTCCTGCCGGATAAGGCGTTCACGCCGGATCCGGCATGAACAAAGCCTACGTTGTCTTCAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACAtt                                                                                                                                 <  1:328923/151‑1 (MQ=255)
                        cacGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATTCGGCATAAGGGGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTg                                                                                                                                <  7:1185572/151‑1 (MQ=255)
                          cGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAAGACCCATACGGCAGGAAGAAAGCCTACGTTCTCTACAATATGAAAATTACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGcc                                                                                                                              >  1:1946370/1‑151 (MQ=17)
                             tCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGTATAAGTCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTg                                                                                                                            <  4:1427313/150‑1 (MQ=255)
                             tCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACTTTGTCTACAATATCAAAATGACCCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTg                                                                                                                            <  5:1866649/150‑1 (MQ=255)
                              cACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGAt                                                                                                                          >  7:967291/1‑151 (MQ=255)
                                cTGCAATCTATTGGATTTGAAGGGTTTTCATACCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATgc                                                                                                                        >  3:1300333/1‑151 (MQ=255)
                                  gCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGACTGATgcgc                                                                                                                      >  1:143558/1‑151 (MQ=255)
                                    attCTTTTGTATTTTAAGGGTTTTCTTTCGGTATTAGTCTTTCACGACGCATCCTGCATGAACAAAGCCTACTTTGTATACAATATTAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTa                                                                                                                    <  6:1480975/149‑1 (MQ=21)
                                    aaTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAACCTGATGACAAACGCAACATTGCCTGATGCGCt                                                                                                                     >  2:75710/1‑150 (MQ=255)
                                    aaTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCt                                                                                                                     >  5:349969/1‑150 (MQ=255)
                                    aaTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGAAGCCTTTTACAGCGCCATTTGAAACTTATGACAAACGCAACATTGCCTGATGCCCt                                                                                                                     >  8:565847/1‑150 (MQ=255)
                                       atATTGGATTTGAAGGTTTTTCATGCCGGATAAGTCTTTCACGCCGCATCCGGCATTAACAAAGTCTTCGTTGTATACAATATTAAAATGCCTCCTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGc                                                                                                                 <  8:1280140/150‑1 (MQ=25)
                                        tATTGGATTTGAAGGGTTTTCATTGCGGATTTGGCTTACACGCCTCATCGGGCATGAAGAAAGCCTTCTTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGc                                                                                                                 <  1:1263665/150‑1 (MQ=18)
                                         aTTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGACTGATGCGCTACGCtt                                                                                                               >  8:641747/1‑151 (MQ=255)
                                             gATTTGAAGGGTTTTCATGCCGGATATCGCGTGCACGCCGCATCCTGCATGAACAAAGCCTACTTTTTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCa                                                                                                           <  1:1540524/151‑1 (MQ=21)
                                             gATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATc                                                                                                            >  7:1525640/1‑150 (MQ=255)
                                                ttGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATCAAAATGACGCCTTTTACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAgg                                                                                                         <  5:1946866/150‑1 (MQ=255)
                                                 tGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGACTGATGCGCTACGCTTATCAGGcc                                                                                                       >  2:1570564/1‑151 (MQ=255)
                                                  gAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGcc                                                                                                       <  7:698265/150‑1 (MQ=255)
                                                   aaGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGAAAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                                      >  2:1260231/1‑150 (MQ=255)
                                                     gggTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCATGATGCGCTACGCTTATCAGGCCTac                                                                                                    >  6:1176009/1‑150 (MQ=255)
                                                     gggTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATCACAAACGCAACATTGCCTGATGAGCTACGCTTATCAGGACTac                                                                                                    >  4:1683125/1‑150 (MQ=255)
                                                       gTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACg                                                                                                 >  5:678976/1‑151 (MQ=255)
                                                         tttCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGt                                                                                                <  8:1884564/150‑1 (MQ=255)
                                                          ttCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTc                                                                                              >  2:1719768/1‑151 (MQ=255)
                                                          ttCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCGTATCAGGACTAAACGtac                                                                                              >  2:1119200/1‑149 (MQ=255)
                                                             aTGCCTTATAAGGCTTTCACGCCTCTTCGGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACt                                                                                           <  4:159464/151‑1 (MQ=25)
                                                             aTGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACATCTAAATTTTAAACTTATGAAAAACCAAACATTGCCTGATGAGATAAGCATATAAGTACTACACTTTCACt                                                                                           >  4:1999157/1‑151 (MQ=14)
                                                             aTGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACt                                                                                           >  2:458086/1‑151 (MQ=255)
                                                             aTGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACt                                                                                           >  6:617033/1‑151 (MQ=255)
                                                                ccGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCATGATGCGCTACGCTTATCAGGCCTACACGTTCACTGc                                                                                         >  6:912032/1‑150 (MQ=255)
                                                                  ggATAAGGCGGCCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAAt                                                                                      <  3:1567530/151‑1 (MQ=255)
                                                                   gATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATc                                                                                     >  7:2037887/1‑151 (MQ=255)
                                                                       aGGCGTTCACGCCTGATGCGGCATTAACAAAGGCTTCGTTGTCTACAATATGAAAATGACGGCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTAtt                                                                                 <  7:517616/151‑1 (MQ=21)
                                                                        ggCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCAATTTGAAACTGATGACAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCAGTGAAATCTaatt                                                                                >  8:1699659/1‑148 (MQ=25)
                                                                          ctttCACGCCGCATCCGGCATGAACAAAGCCTTCGTTGTCTACAATATTAAAATGACGCGTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTg                                                                                <  1:95007/147‑1 (MQ=255)
                                                                           gTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCTCCAAATCTAACAGATGACACACGCAACATAGACTCATGAGCTACGCTTATCAGGCCGACAAGGACAATAAAATATATTGGAt                                                                             >  4:843007/1‑151 (MQ=17)
                                                                              naNGCCGCNTNCGGCATGANNAAAGCCTACGTTGTANACAATNTGAAAANGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAANGCNACATTGCCTGATGCGCNACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTg                                                                          <  7:433670/150‑1 (MQ=21)
                                                                                     gaTCCGGCATGAACAAAGCCTACGTTGTATACAATATGAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGtt                                                                   <  8:1121155/150‑1 (MQ=255)
                                                                                        ccGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTc                                                                >  1:1131146/1‑151 (MQ=255)
                                                                                                  cAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGAt                                                       >  3:134942/1‑150 (MQ=255)
                                                                                                   aaaGCCTTCTTTTTATTCAATATTAAAATGTCTCTTTATAAAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATaa                                                     <  4:244446/151‑1 (MQ=11)
                                                                                                    aaGCCTACTTTTTTTTAAATTTTAATATGACGCCTTTTATATAGCCATTTGAATATTATGAAAAACTCAACTTTGCTTTATGCTTTTCGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAg                                                    <  6:1852326/151‑1 (MQ=9)
                                                                                                    aaGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATTAAAAACAAAACATTGACTGATGAGCTACGATTATCAGAACAAAACGTTCACAGCAATATATTTGATTTGAAGGGTTTTCAttaaagata                                                      >  6:1377215/1‑141 (MQ=21)
                                                                                                    aaGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAg                                                    <  3:2025289/151‑1 (MQ=255)
                                                                                                       ccTACTTTTTATACAATAAGAAAATGACACATTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCg                                                 <  3:1173284/151‑1 (MQ=14)
                                                                                                          aCGTTGTCTACAATATGAAAATGACGGCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGt                                                <  1:843082/149‑1 (MQ=255)
                                                                                                           cGTTGTCTACAATATGAAAATTTTTTATTTTATAGCGCCATTTGATACTTATTAAAATCGCAACATTGCCTTATGCTATACGATTATCATGCGTTCTCGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCa                                             <  2:1946137/151‑1 (MQ=9)
                                                                                                           cGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTc                                              >  2:972941/1‑150 (MQ=255)
                                                                                                            gTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAc                                            >  7:1019713/1‑151 (MQ=255)
                                                                                                                tCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGTATAAGGCGTTCAcgccg                                        >  6:2041233/1‑151 (MQ=255)
                                                                                                                tCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccg                                        >  2:270648/1‑151 (MQ=255)
                                                                                                                 cTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccgc                                       >  3:842461/1‑151 (MQ=255)
                                                                                                                 atACAATATGAAAATGACTCGTTTTACAGCGCCATTTAAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccg                                        <  5:899202/149‑1 (MQ=255)
                                                                                                                   aCAATTTGAAAATTACGCCGTTTACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                     <  1:64227/151‑1 (MQ=255)
                                                                                                                   aCAATATGAAAATGTCGCCTTTTAGATCGCGATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCAcgccg                                        <  4:1892737/148‑1 (MQ=14)
                                                                                                                   aCAATATCAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCa                                      <  7:1698277/150‑1 (MQ=255)
                                                                                                                    cAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATc                                    >  4:643731/1‑151 (MQ=255)
                                                                                                                     aaTATTAAAATGACTCCTTTTACAGCCCCATTTTAATATGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATcc                                   <  2:1515334/151‑1 (MQ=14)
                                                                                                                     aaTATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGACGGATAAGGCGTTCACGCCGCATcc                                   >  4:1068857/1‑151 (MQ=255)
                                                                                                                      atatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAGTCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCg                                  >  5:1419047/1‑151 (MQ=255)
                                                                                                                       tatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCgg                                 >  7:1316535/1‑151 (MQ=255)
                                                                                                                           aaaaTGACGCCTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCATTCACGCCGCATCCGGCATg                             <  3:725516/151‑1 (MQ=255)
                                                                                                                             aaTGCCGCCTTTTACAGCGCCATTTGAAACTGCTCACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa                           <  7:611607/151‑1 (MQ=255)
                                                                                                                              aTGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc                          <  5:1715846/151‑1 (MQ=255)
                                                                                                                                gACGCCTTTTACAGCGCCATTTCAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACa                         <  5:544771/150‑1 (MQ=255)
                                                                                                                                  gtctttttATATCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAg                      <  6:419222/147‑1 (MQ=255)
                                                                                                                                       tttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTAc                 >  5:1526720/1‑151 (MQ=37)
                                                                                                                                        ttACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTAc                 <  4:380865/150‑1 (MQ=21)
                                                                                                                                        ttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACg                >  3:1694997/1‑151 (MQ=37)
                                                                                                                                         tACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACg                <  6:760422/150‑1 (MQ=18)
                                                                                                                                         tACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGt               >  7:1388808/1‑151 (MQ=35)
                                                                                                                                         tACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGt               >  1:509510/1‑151 (MQ=35)
                                                                                                                                          aaagagactttttAAAATGATGACTCTCTCAAGATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGt               <  2:869422/141‑1 (MQ=11)
                                                                                                                                          aCAGCGCCATTTTATACTGATTACATACGCTACATTGCTTTATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGtt              <  6:1137269/151‑1 (MQ=11)
                                                                                                                                          aCAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGtt              <  1:1678929/151‑1 (MQ=35)
                                                                                                                                          aCAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGt               <  7:1882506/150‑1 (MQ=35)
                                                                                                                                           cAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTg             >  3:607894/1‑151 (MQ=34)
                                                                                                                                            aGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGt            >  6:165733/1‑151 (MQ=33)
                                                                                                                                             ccgcCATATGAAACGGATCACACACGCAACATTGCCGGACGCCCGACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTc           <  1:258287/150‑1 (MQ=11)
                                                                                                                                              cgcCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCt          >  3:553564/1‑151 (MQ=33)
                                                                                                                                              cgcCATTTGAAACTGATGACAAACGCAAAATTGCCTCATGCGCTACGCTTATCAGCCCTACACGTTCACTGCAAACTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGATTTAACGCCGCATCCGCAATGACCAAAGACTACGATGTCt          >  6:533957/1‑151 (MQ=11)
                                                                                                                                               gcCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTa         <  8:1383075/151‑1 (MQ=14)
                                                                                                                                                ccATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTAc        >  4:1237184/1‑151 (MQ=33)
                                                                                                                                                   tttGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAat     >  5:1167961/1‑151 (MQ=33)
                                                                                                                                                      |                                                                                                                                                      
GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATG  >  DH1/2326137‑2326437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑