Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,591,5130TG24.5% 251.5 / 37.0 226D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (83/62);  new base G (19/28);  total (116/110)
Fisher's exact test for biased strand distribution p-value = 6.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

CCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATGAG  >  DH1/2591365‑2591662
                                                                                                                                                    |                                                                                                                                                     
cccgaatttcGAACCGCTCAGCGCTGACTAATCATTACCACACCACAATAACTTTAAAAAGTCCATGACGTCTTAAGCTAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCa                                                                                                                                                     <  4:1012010/142‑1 (MQ=17)
  cGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGGCTGGAGCGGCAGc                                                                                                                                                   >  1:1159204/1‑151 (MQ=255)
     cgggCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCgag                                                                                                                                                <  7:1147051/150‑1 (MQ=255)
              cgcgcAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGACCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTTGAGCGCCaacatccgagccgcc                                                                                                                                       >  1:958327/1‑137 (MQ=11)
                cgcAGCGCTGAGAAACCCTTCCCCCACAACCATCACTATCAACCCACCCTCAACTCTCACCCGACACGGCACTCAACTCTTCTGCCCATCCGCTGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGcc                                                                                                                                     <  7:1444758/151‑1 (MQ=255)
                 ccAGCGCTGAGTAATCCTTCCCCCACCATAATCACTTTCACAAGTCCCTGAACTCTAAACCGAATCCGCAATCAAATATTCTCCCCCTGCGGGTAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGTCAGCGAGAGAGCGTTGCCg                                                                                                                                    <  6:1836861/150‑1 (MQ=14)
                   cccgcggaccaaTCCTACCCCCAACACCATCCCTTACAAACGCCCCTGACCTCTCCAGCGCAGCCGCCAGCAAATATGCTGCCCCTGCCGCGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCa                                                                                                                                  <  5:1507932/141‑1 (MQ=9)
                   aGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTGGAGCGGCAGCGAGAGAGCGTTGCCGc                                                                                                                                   >  4:272122/1‑150 (MQ=255)
                      ttttattaatgtttCCGCCACCTGCTTCTCTTTCAAATGTGCCTGAACTGTCTATCGAATCGGCAATTAAATTTTCTGCGCTTGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTGGGCTCGAGCGTCAGCGAGAGAGCGTTGCGGCAgg                                                                                                                                <  4:673318/138‑1 (MQ=2)
                         ttctAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAg                                                                                                                                 <  4:1702340/143‑1 (MQ=255)
                          tgTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAACGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAAc                                                                                                                            <  6:537451/149‑1 (MQ=255)
                          agtaagcgcacaccccccACCATCCCATCCAAAAGTCCCTGAACTCCCCACCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACg                                                                                                                           <  1:1682151/139‑1 (MQ=11)
                            gaaTCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCgag                                                                                                                                                <  8:1167571/127‑1 (MQ=255)
                            caaTCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGAc                                                                                                                         <  1:1586458/150‑1 (MQ=255)
                                ccTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGACTTGAGCGGCAGCGAGAGAGCGTTGCCgagatcggaagacccc                                                                                                                     >  7:1850016/1‑136 (MQ=11)
                                   tCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTAGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAAcacaacgga                                                                                                                    >  4:816441/1‑141 (MQ=14)
                                   tCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCGCAACCGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGaa                                                                                                                    <  1:585090/149‑1 (MQ=255)
                                    cccccACCACAATCACTTTCAAAAGTCCCTGAACTTTCAAGCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAggg                                                                                                                 <  4:1242054/151‑1 (MQ=255)
                                     ccccacaaatataaatttAAAACGTCCCTCAAATCTCAACCGAATCCCAAATAAAATTTTCTGCCACTGCGGGGAAGGTTTATAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCTATAGAGCGTTGCCGCAGGCAACGACCCGAAGGGc                                                                                                                <  2:1664598/136‑1 (MQ=9)
                                       ccaccaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCg                                                                                                               >  2:478609/1‑150 (MQ=255)
                                       ccaccaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGCAGGACAGCCTACGAGTAAGCTGAGCATCCACCGACCGCTAGCGAGCGTTGCCGCAGGCAACGAccccatccccg                                                                                                               >  7:813314/1‑142 (MQ=9)
                                        caccaccaTCACTTTCAAAAGTCCCTGAACTCTCCATCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGaa                                                                                                             <  2:1419709/151‑1 (MQ=255)
                                          ccaccaTCACTTTCAAAAGTCCCTGAACTTTCAAGCTAATCCGCAATTAAATATTCTGCCCATGCGGGTAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgc                                                                                                           <  2:1246097/151‑1 (MQ=255)
                                          ccaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgc                                                                                                           >  8:1423208/1‑151 (MQ=255)
                                            accaTCACTTTCAAAAGTCCCTGAACTCCCAACCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGATTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgc                                                                                                         <  3:690324/151‑1 (MQ=255)
                                              caTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgcgc                                                                                                       >  1:1217029/1‑151 (MQ=255)
                                               aTCACTTTCAAACGTCCGTGAACTCGCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGCGCGTCAGCGAGAGAGGGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCa                                                                                                      <  5:854240/151‑1 (MQ=14)
                                               aTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCACGTGAGCGGAAGGCTGAGACGGATCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGCCCCGAAGGGCGAAgcgcgcg                                                                                                      >  3:864512/1‑150 (MQ=14)
                                                tCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgcgc                                                                                                       >  2:1729490/1‑149 (MQ=255)
                                                tCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgcgc                                                                                                       >  7:615479/1‑149 (MQ=255)
                                                     ttCAAACGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCTAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcgc                                                                                                  <  6:1871901/149‑1 (MQ=255)
                                                     ttCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                                                                                                >  4:1669219/1‑151 (MQ=255)
                                                     ttCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                                                                                                >  7:1020310/1‑151 (MQ=255)
                                                     ttCAAAAGCCCCTGAACTCCCAACCGAACCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGACGCTGCGACTAAGGTGCGGCGCCAGCGCCAGCGAGAGAGCGTGGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCt                                                                                                 <  1:1981334/150‑1 (MQ=11)
                                                      tCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                                                                                                >  5:1795625/1‑150 (MQ=255)
                                                         aatttCCCTGAACTTTCAACGTAATCCGAAATTAAATATTCTGCCCATGCGGGGAAGGATTATAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGGGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTa                                                                                            <  8:580172/147‑1 (MQ=11)
                                                         aaaGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTa                                                                                            <  6:1317841/151‑1 (MQ=255)
                                                           aGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAAt                                                                                          >  4:1100172/1‑151 (MQ=255)
                                                           aGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCAGCCCATGCTCGGAAGGATGAGAAGCATCGACTAAGGATCGGCTCGAGCGTCAGCGAGAGCGCGTTGAAGAAGGCAACGACCCCAAGGGCGAAGCGCGCAgggcgccggctg                                                                                          >  8:1574998/1‑143 (MQ=255)
                                                            gTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTTGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATc                                                                                         <  6:1794496/151‑1 (MQ=255)
                                                            gTCCCTCAACTCTCAACCGACTCAGCAATCAAATATTCTGCCCTTGCGGGTAAGGATGTGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATc                                                                                         <  4:598941/151‑1 (MQ=14)
                                                             gccgttaaCTCTCAAGCGAAGCCGCAATCAAATATTCTGCCCATGCGGGGACGGATGAGAAGCTGCGACTACGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATcc                                                                                        <  6:1442928/145‑1 (MQ=14)
                                                              cccTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCt                                                                                       >  8:413571/1‑151 (MQ=255)
                                                               ccTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCtt                                                                                      >  6:1197513/1‑151 (MQ=255)
                                                                cTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTc                                                                                     >  1:1702287/1‑151 (MQ=255)
                                                                  taagattcccatgAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTcc                                                                                    <  5:135787/138‑1 (MQ=11)
                                                                  gAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGCCTAGAGCGACAGCGAGAGAGCGTTGCCGCAGGCaa                                                                                                                             >  4:1553946/1‑109 (MQ=14)
                                                                     ctctCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTAGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTcccccc                                                                                >  8:480131/1‑150 (MQ=255)
                                                                     ctctCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTAGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccccc                                                                                 >  6:707686/1‑150 (MQ=255)
                                                                      tctcAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGa                                                                                                                          >  3:387577/1‑108 (MQ=255)
                                                                       ctcAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACg                                                                                                                           >  3:1265972/1‑106 (MQ=255)
                                                                         cAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCCGCGCTGAGTAATCCTTCCCccaccac                                                                            >  5:1426041/1‑151 (MQ=255)
                                                                         cAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTAGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGaa                                                                                                                    >  1:72717/1‑111 (MQ=255)
                                                                           aGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTAGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCttagatcggaagcc                                                                          >  1:103896/1‑139 (MQ=11)
                                                                            cccaatcttaaacaaaaTATCCCGCCCATCCGGGTAATGATGCGAAGCTGCGAGTAAGGTGGGGGGCGCGCGGCAGCTAGAGCGGGTGGCCGCAGCCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCAt                                                                         <  3:2034228/137‑1 (MQ=2)
                                                                                  ccGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATcgc                                                                      >  1:1401766/1‑146 (MQ=38)
                                                                                    gCAATCAAATCTTCAGCCCATAAGGGGCCGGATGTGGAGGTCAGACTATGGTTCTGCCGTTGGGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCa                                                                 <  1:1622140/151‑1 (MQ=11)
                                                                                     cAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCttactt                                                                >  2:850205/1‑147 (MQ=255)
                                                                                      aaTCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCTTCCat                                                                >  4:1880861/1‑149 (MQ=255)
                                                                                      aaTCAAATATTCTGCCCATGCGGGGAAGGATGAGAACCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCTTACat                                                                >  2:1113296/1‑149 (MQ=255)
                                                                                        tCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGACTAGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCTTACaaaa                                                              >  8:950942/1‑150 (MQ=255)
                                                                                          aaaTATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCa                                                                                                                                  <  2:51547/80‑1 (MQ=255)
                                                                                           aaTATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCTAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGccc                                                          <  5:202684/151‑1 (MQ=38)
                                                                                            atatTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCttgcttaagcacc                                                         >  7:2023784/1‑140 (MQ=11)
                                                                                              atTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCgagaga                                                                                                                                             <  2:230011/65‑1 (MQ=255)
                                                                                              atTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGTCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCgg                                                       <  6:1558802/151‑1 (MQ=25)
                                                                                                 gtGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAAt                                                    <  2:1376952/150‑1 (MQ=255)
                                                                                                 ggccccAGGGGGGTAAGTATGACACGCTGCGAAAACGGTGCGCCGGGAGCGCCAGCTATAGTGGTTTGCCGCCGCAAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAAt                                                    <  7:443032/148‑1 (MQ=2)
                                                                                                  tGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTAGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCTCTTACaaaaataaacaaaa                                                    >  8:296553/1‑140 (MQ=11)
                                                                                                  aggacgctcttgctaGGTTGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAAtt                                                   <  6:2058999/137‑1 (MQ=14)
                                                                                                    cccATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtct                                                 <  3:1167939/151‑1 (MQ=255)
                                                                                                     caaTGCGGGGAAGTTTGATATGCTTCGACTAAGGTTCGTCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGAAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtctc                                                <  6:75910/149‑1 (MQ=14)
                                                                                                        tGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCAt                                                                         >  6:163505/1‑123 (MQ=37)
                                                                                                           ggggAAGGATGAGAAGCTTCGACTAAGGTTCGTCTGGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCAt                                                                         <  4:1087994/120‑1 (MQ=12)
                                                                                                           ggggAAGGATGAGAAGCTTCGACCAAGGTTCGCCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGa                                          <  5:489074/151‑1 (MQ=16)
                                                                                                            gggAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGaa                                         <  5:142000/151‑1 (MQ=255)
                                                                                                            gcgtaGGTTGAGATGTTTCGACAAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGaa                                         <  4:1958205/147‑1 (MQ=11)
                                                                                                             ggctctaggccacgCTTCGACGAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAAt                                        <  3:1097904/138‑1 (MQ=14)
                                                                                                               aaGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGACAGCCAGAGAGCCTTGCCGCACGCAACGCCCCGCACCGCGAAGCGCCCAGCGCAGAGTAATCCTTCACACACCACCATCACTTTCAACAGCCCCGGAATTCTCAAACGACTcc                                      >  5:747199/1‑151 (MQ=255)
                                                                                                                   aTGAGAGGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCa                                   >  3:1709150/1‑150 (MQ=255)
                                                                                                                   aTGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACGACCATCACTTTCAAAAGCCAAGCAATTCTAAAACGAATCCGCa                                   >  8:76331/1‑150 (MQ=255)
                                                                                                                     gagaTGCTTCGACAAAGGTTGGTCTCGAGCGCCAGCTATAGAGCGTGGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAAt                                 <  8:1796464/150‑1 (MQ=11)
                                                                                                                     gagaAGCTTCGACTAAGGTTCGTCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAAt                                 <  8:1634928/150‑1 (MQ=255)
                                                                                                                     gagaAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCGAGGAACCCCCACCAGGACGGTCAAAAGCGCCGGACGTCTGAAACGCAGCCGccagc                                >  6:86230/1‑147 (MQ=11)
                                                                                                                       gacgCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCa                               <  7:1295513/147‑1 (MQ=255)
                                                                                                                         aGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCCCCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAat                            >  7:78115/1‑151 (MQ=255)
                                                                                                                            ttCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGGATAATCCTACCCCCACCACCAGAACTTTCAAAAGCCCCGGAATTCTAAAACGAGTCCGCAATCAAATAtt                         >  4:786605/1‑151 (MQ=14)
                                                                                                                              cGACCAAGGTTCGTCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCt                       <  4:176740/151‑1 (MQ=255)
                                                                                                                                acaaaGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGc                     <  3:1142488/148‑1 (MQ=255)
                                                                                                                                aCTAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGc                     <  5:572218/151‑1 (MQ=255)
                                                                                                                                 cgaaGGTTCGTCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGc                     <  4:409950/148‑1 (MQ=255)
                                                                                                                                   aaGGTTCGGCTCGCGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCa                  <  8:78003/151‑1 (MQ=255)
                                                                                                                                    ccgttcttcTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCaa                 <  6:1356687/143‑1 (MQ=255)
                                                                                                                                     ggTTCGTCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAg                <  5:1026680/151‑1 (MQ=255)
                                                                                                                                     ggTTCGGCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAg                <  1:792547/151‑1 (MQ=255)
                                                                                                                                     ggTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAg                <  3:875323/151‑1 (MQ=25)
                                                                                                                                     ggTGCGGCTCCTACGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCa                  <  3:828335/149‑1 (MQ=255)
                                                                                                                                         cGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCggg            >  7:1644479/1‑151 (MQ=255)
                                                                                                                                          ttcTCGTGCGTCAGCTATAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCggg            <  4:2027146/148‑1 (MQ=255)
                                                                                                                                               gAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGGGCAGCGCTGAGTAATCCTTCCCCCACCCCCATCACTTTCAAAAGCCCCGGAATTCTCAAACGACTCCGCAATCAAATATTCTGCCCAAGCGGGGAAggt      >  5:1593347/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATGAG  >  DH1/2591365‑2591662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑