Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,184,5850CA25.0% 406.2 / 51.3 168intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
Reads supporting (aligned to +/- strand):  ref base C (72/54);  new base A (36/6);  total (108/60)
Fisher's exact test for biased strand distribution p-value = 7.29e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

ATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184438‑3184734
                                                                                                                                                   |                                                                                                                                                      
  cccGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATAAAAG‑a                                                                                                                                                   >  3:1323168/1‑150 (MQ=255)
    cgtcgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAG‑aacc                                                                                                                                                >  3:997244/1‑148 (MQ=255)
      gcggacccacctATTTCTTAATGTAGCGAAACGGTTCGGCAATATCCATAGGACATTGTTGGGGACTCGCCACGCGGTAAGCCACCGGTTTGTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCtt                                                                                                                                              <  7:341228/145‑1 (MQ=12)
       cgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAG‑aaccat                                                                                                                                              >  5:387983/1‑145 (MQ=255)
           gCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTAATAAAgcaccgtacggg                                                                                                                                          >  1:23753/1‑140 (MQ=21)
             caccaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAG‑CAGCGTACCtcg                                                                                                                                        >  1:412619/1‑148 (MQ=21)
              cccaactgccgAATGTATCGATTATGTTCTGCAAATGCACACCGAATTTGTTGTCCTATCGCGACCCTGTATTTCAGAGGTTTCTTATTCCGTCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTa                                                                                                                                      <  1:1307527/141‑1 (MQ=255)
              accaAATTCTGAATGTATCGAATATGTTCGTCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTa                                                                                                                                      <  4:274002/151‑1 (MQ=255)
              accaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTa                                                                                                                                      <  8:1800819/151‑1 (MQ=255)
              accaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAG‑ATGGCTACCTGGtc                                                                                                                                      >  7:1215218/1‑150 (MQ=21)
               ccaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAG‑aacccttcggggta                                                                                                                                      >  5:779324/1‑137 (MQ=21)
                caAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAATAAAGCACGCttcgtagagcg                                                                                                                                     >  1:231172/1‑142 (MQ=18)
                             tATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAG‑ACCCTTACCTTGTCAGTTCTGCAAGTTgg                                                                                                                       >  7:505989/1‑151 (MQ=21)
                                 gAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAAAAAAG‑AGGGGTACCTTGTGAGTggtccgtgtgtgcgtt                                                                                                                   >  1:574954/1‑136 (MQ=18)
                                    tatGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAG‑AACCGTACCTGGTCAGTGCACCGAGTTTGCGTAGCg                                                                                                                >  7:651629/1‑151 (MQ=21)
                                        taacgCAAAATCAACTCCAATTTGTTGGGGTATCTCCAAGCTGTAATCCACCTGCTTCTTAATCTGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCa                                                                                                             <  8:794605/146‑1 (MQ=25)
                                          cGGCAAATTCAAAAACAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGc                                                                                                          <  2:448039/151‑1 (MQ=255)
                                          cGGCAAATACAAAACCACGTTGTAGGGGTCTGCCCACTCGGGAATTCACAGCATTCTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGc                                                                                                          <  1:1604885/151‑1 (MQ=25)
                                             ccccttcaccccccgttttttTGGGTATCGCCAAGCGGTAAGGCACCGGATTGTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGt                                                                                                       <  3:1973130/136‑1 (MQ=25)
                                             cAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAG‑ACGCTTTCCTAGAAATTGCACCCAGTGTGGGttgctgcaagctgg                                                                                                       >  3:922733/1‑138 (MQ=18)
                                               aaTTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTaa                                                                                                     <  5:675721/151‑1 (MQ=255)
                                                 ttCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAATAAAG‑AAGCGTACCTGGAGAGTGATCCTATTCGGGGTATCTACAATCGCTaatg                                                                                                   >  5:1316981/1‑149 (MQ=14)
                                                  tCAAACACAATTTGTTGGTTTCTCCCCCATCTTAAATGCACCTGATTCTGATACCGGCTATCCGAGGTTCGAATCCTCGTACCCCAGACACTTTATTCAAG‑CCGCTTCACTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAgg                                                                                                   <  5:891610/150‑1 (MQ=19)
                                                  tCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAG‑ACCCTTACCTTGTCATTGAACCAAGTTTGGGTATAGCCAAGCTGTAAGGc                                                                                                  >  5:585495/1‑151 (MQ=21)
                                                     aacgcAATATGTGGGGGTCTCGCCAACCGGTAAGGCACCGGATTCGGATTCCGGCACTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTTCAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGc                                                                                                  <  7:1572527/144‑1 (MQ=25)
                                                        cccgtttATTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACg                                                                                                                                                 <  3:848634/94‑1 (MQ=255)
                                                         ccttttATTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTAt                                                                                                                  <  1:635916/125‑1 (MQ=255)
                                                         ccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAC‑ACGCTTACCATGTAAGTACACCCAGTTGGGGAAt                                                                                                                  >  2:635851/8‑128 (MQ=17)
                                                            tttCTTGGTGTATCTCCACTCTTTAAGTCAACGGATTGTGTCACAGGTATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTc                                                                                         <  8:63259/150‑1 (MQ=255)
                                                              tattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCGTGGTTCGAATCCTGGTACCCCAGCCAATTAATTCAAG‑ACGCTTCCCTTGTAAGTGc                                                                                                                                 >  3:1574723/3‑108 (MQ=17)
                                                              tattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGc                                                                                                                                 <  4:1574091/106‑1 (MQ=255)
                                                                  ggggTATCGCCAAGCGGTAAGGCACCGGTTTATGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTCAAG‑Acc                                                                                                                                                 >  5:1852422/1‑87 (MQ=14)
                                                                  ggggTATCGCCAAGCGGTAAGGCACCGGATTGTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACg                                                                                                                                                 <  6:1851209/88‑1 (MQ=255)
                                                                   gtggaTCGCCACCCGGTAAGGCACCTGATGCGGTTTCCGGGATTCCGCGCTTCGAAGCCTCGTACCCCAGCCACTTTATTCAAG‑ACTCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCg                                                                                 <  5:1964661/147‑1 (MQ=37)
                                                                     gTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑Ac                                                                                                                                                  <  1:179394/84‑1 (MQ=255)
                                                                          gCCAAGCTGTAATGCACAGGATTCTGATTCAGGCATTACGAGCTTCGAATCCTCGTACCCCAGCCAATTTATTAAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTcc                                                                          <  8:459704/151‑1 (MQ=38)
                                                                           ccatcctgtaatcaaCCGGTTTGTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGACAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCg                                                                         <  8:1125880/137‑1 (MQ=37)
                                                                           ccAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑AAGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGAgg                                                                                                        >  3:1641160/1‑120 (MQ=25)
                                                                           ccAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAAGTTATTCAAG‑ACGCTTACCTTGTAAGGGCACCCAGTTGGGGTAt                                                                                                                  >  5:1913651/1‑110 (MQ=21)
                                                                           ccAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCgg                                                                                                        <  4:1640380/120‑1 (MQ=255)
                                                                            cccgccggacgccACCGGATGCTGACTCCGGCACTCCGCGCTTCGTATCCTCCTACCCCAGCCAATATATTCAAT‑ACGCGTCCCTTGTAAGTCCACCATGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGa                                                                        <  5:1921137/139‑1 (MQ=21)
                                                                             aatctttaatgaaCCTGATTCTGTCTCAGGCATTAATAGGTTCGTATCCTCGTAATCCAGAAATTTTATTCAAT‑TATCTTAAATTGTAATTGCACTCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAg                                                                       <  4:1345487/139‑1 (MQ=12)
                                                                             aaGCGGTAAGGCTACTGATTCGGTCTCCGGCTCTCATCCGTTCGAATCCTCGTACCCCAGCCACTTTATTAAAG‑CCGCTTACCTTGTAACTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAg                                                                       <  3:431011/151‑1 (MQ=21)
                                                                             aaGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGATTACCTTGTAAGTGCACCCAGtt                                                                                                                         >  1:56193/1‑101 (MQ=18)
                                                                             aaGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCa                                                                                                                                                       >  4:1521764/1‑72 (MQ=14)
                                                                             aaGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAG‑AACCGTACCTTGTAAGTGCACCCAGTTTGGGTATCTCCAATCGGTAAGGCACAGGATTCTAATTCCTGCATTcag                                                                         >  7:430828/1‑147 (MQ=25)
                                                                               cgcgtacgccaacGGATTGTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTAACTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGt                                                                     <  1:475726/139‑1 (MQ=37)
                                                                                  tcacggaaactgATGCTGAATCCGGCTCTCATCGGTTCGAATCCTCGTAACTCTGCAAATATATTATAG‑ACGCTTAACTTTCAACAGCAACCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAgg                                                                      <  3:1847833/136‑1 (MQ=12)
                                                                                  gTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAG‑AAGCATACCTTGTAAGTGAACCCAGTTTGGGTATCGACAAGCTCTAAGGCAAAGGATTCTAATTAAGTCATTCCGAGGTTcc                                                                  >  8:447318/1‑150 (MQ=21)
                                                                                   tAAGGCACCGGTTTTTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGt                                                                                                                          <  6:1053637/94‑1 (MQ=255)
                                                                                      cccACCGGATGCGGATACCGGCACGCCGAGGGTCGAAGCCTCGTACCCCAGCATTTTTATTATAG‑CCGCTTCCCTTTTAAGTGCACACTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATc                                                              <  6:929652/149‑1 (MQ=18)
                                                                                       gCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCAGCCAGTTTGGGTATCGACAAGCTGTAATGCAACGGATTCTACTTCCGGCATTCCGAGGTTCGAATc                                                              >  3:651838/1‑150 (MQ=37)
                                                                                           cGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTACTTCCGGCATTCCGAGGTTCGAATCCTCGt                                                         >  3:1011829/1‑151 (MQ=255)
                                                                                            ggATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGtc                                                        >  3:1005312/1‑150 (MQ=255)
                                                                                             gtttttGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTAt                                                                                                                  <  5:1166720/87‑1 (MQ=255)
                                                                                             gtttttGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTAt                                                                                                                  >  6:1165640/6‑92 (MQ=25)
                                                                                                  tGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCtt                                                                                                                                         <  7:1739838/64‑1 (MQ=255)
                                                                                                             ttCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAG‑CACCTTACCTAGTAAGTGCACCCAGTGGGGGTATCGACAAGCGGTAAGGCCCCGGATACTAATTCAGGCCTTCCGAGTATCGACACAGCGTACCCCAGCCACAATaaa                                        >  4:1595461/1‑150 (MQ=255)
                                                                                                                cGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTACGTGCACCCAGTTGGGGTATCGCCAAGCGCTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGATCGCAGCCTCCTGCCCCAGCCACATTaaaaaac                                    >  3:304891/1‑150 (MQ=255)
                                                                                                                   ggTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCATACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTc                                 >  5:811139/1‑151 (MQ=255)
                                                                                                                     ttCGAATCCTCGTACCCCAGCCAATTTATTAAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGAGTCTAATTCCCGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGc                               >  1:1608200/1‑151 (MQ=255)
                                                                                                                     cgcgcagccacccaCCCCAGCCACTATATTCAAG‑TTTCTTACCATGAAACTCCACCATGTTGGCGTATCGCCACGCGGTCAGGCACCGGATTCTCATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGc                               <  1:837399/138‑1 (MQ=12)
                                                                                                                        gAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            >  3:743037/1‑151 (MQ=255)
                                                                                                                        gAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCAGAGGTTCGAATCCTCGTCCCCCAGCCACATTAAAAAAGCTCGCtac                            >  1:1118811/1‑149 (MQ=255)
                                                                                                                        gAATCCTCGTACCCCAGCCAATTTATTCAAG‑AAGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTAGCTTc                            >  8:1546474/1‑151 (MQ=255)
                                                                                                                         cagcctccaaCCCCAGCCAATTTATTCAAT‑TTTCTTAAATTTAAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTTATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCg                           <  6:536164/142‑1 (MQ=18)
                                                                                                                          aTCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCgg                          >  8:1706288/1‑151 (MQ=255)
                                                                                                                          aTCCTCGTACCCCAGCCAATTTATTAAAG‑ACGCATACCTTGTAAGTGCACCAAGTTGGGGTATCGACAAGCGGTAAGGCACCGGATTCTAATTACGGCATTCCGAGTTTCAAATCAACGTACCCCCGCGACATTAAAAAAGCTAGCTTAgg                          >  2:1380797/1‑151 (MQ=25)
                                                                                                                            ccacGTACCTCAGACTATATATTCAAG‑GCGCTTCACATTCAAGAGCACTCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        <  7:158090/148‑1 (MQ=17)
                                                                                                                            ccacGTAACCCAGACAATTTCTACAAG‑ACGCTTACCTTGTAAGTGCAAGTAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGc                         <  7:62565/147‑1 (MQ=21)
                                                                                                                             cTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCgc                       >  7:1976674/1‑150 (MQ=255)
                                                                                                                                gTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCt                    >  8:776286/1‑151 (MQ=255)
                                                                                                                                 tACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtt                   >  1:1115524/1‑151 (MQ=255)
                                                                                                                                 tACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCAAGTTGGGGTATCGCGAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCGTCGGCGAGCtt                   >  3:1726144/1‑151 (MQ=255)
                                                                                                                                 aaCCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtt                   >  7:9286/2‑151 (MQ=255)
                                                                                                                                  aCCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  6:1847408/1‑151 (MQ=255)
                                                                                                                                  aCCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  7:293527/1‑151 (MQ=255)
                                                                                                                                   ccccAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtttt                 >  6:114074/1‑151 (MQ=255)
                                                                                                                                      cAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTgg              >  8:2043004/1‑150 (MQ=255)
                                                                                                                                      cAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGc              >  5:1087011/1‑151 (MQ=255)
                                                                                                                                      cAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGc              >  7:1298946/1‑151 (MQ=255)
                                                                                                                                       aGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt             >  1:672057/1‑151 (MQ=255)
                                                                                                                                       aGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt             >  5:1550491/1‑151 (MQ=255)
                                                                                                                                         ccAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCttt           >  2:125291/1‑151 (MQ=255)
                                                                                                                                         ccAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCttt           >  2:1009287/1‑151 (MQ=255)
                                                                                                                                          cAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  8:1852019/1‑151 (MQ=255)
                                                                                                                                           aaTTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTc         >  4:825320/1‑151 (MQ=255)
                                                                                                                                            aTTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        >  7:1777194/1‑151 (MQ=255)
                                                                                                                                             tttATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTg       >  5:1620526/1‑151 (MQ=255)
                                                                                                                                             tttATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTg       >  5:639204/1‑151 (MQ=255)
                                                                                                                                              ttATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCAGGCATTCCGAGTTTCGAATCGTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGGTTTTCTGc      >  7:908005/1‑151 (MQ=255)
                                                                                                                                              ttATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGc      >  5:1961151/1‑151 (MQ=255)
                                                                                                                                              ttATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGc      >  3:1600748/1‑151 (MQ=255)
                                                                                                                                                cgtCAAG‑CCGCTTCCCATTGAACAGCACGCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     <  7:1611180/148‑1 (MQ=21)
                                                                                                                                                aTTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGt    >  8:253603/1‑151 (MQ=255)
                                                                                                                                                 ttCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGtt   >  3:578890/1‑151 (MQ=255)
                                                                                                                                                  tCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  6:1753290/1‑151 (MQ=255)
                                                                                                                                                  tCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  7:1100842/1‑151 (MQ=255)
                                                                                                                                                  tCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  3:382209/1‑151 (MQ=255)
                                                                                                                                                   cAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTCCTTTTCTGCGttc  >  6:193610/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
ATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAG‑ACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184438‑3184734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑