BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	3,448,526	0	A	C	27.9%	778.0 / 114.3	430	intergenic (‑86/+70)	malZ/proY	alpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand): ref base A (243/64); new base C (3/116); total (246/184)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 445 total aligned reads displayed.

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676

      tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggggg                                                                                                                                                  >  8:370821/1‑151 (MQ=255)

         tCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCgg                                                                                                                                               >  3:1034485/1‑151 (MQ=255)

           tttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGAt                                                                                                                                             >  1:974907/1‑151 (MQ=255)

            ttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATTCATTTAACATCATAATTCCCCTGTTATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCgg                                                                                                                                               <  4:1581096/148‑1 (MQ=255)

              gctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAAc                                                                                                                                          >  7:1446327/1‑151 (MQ=255)

                tttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACtt                                                                                                                                        >  4:1246780/1‑151 (MQ=255)

                    tttAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCgg                                                                                                                                    >  6:581002/1‑151 (MQ=255)

                       aagaattGGGGGCAGCGGCAGGTGCCATGAATTTAACATCATAATTCCCCTGTTATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAAt                                                                                                                                 <  1:1303003/145‑1 (MQ=255)

                        aCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAAt                                                                                                                                 >  5:353230/1‑150 (MQ=255)

                         cAACTGGGGGCACCGGCCGGGGCCATTCATTTAACATCAAAATGCCCCTGTGATTAAAATCTGATCCCCTTGCCATATCCTAATGCAGTATCACTCATCATCTGAAGTATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAg                                                                                                                               <  8:1705200/151‑1 (MQ=255)

                           aaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGcc                                                                                                                             >  4:594341/1‑151 (MQ=255)

                            ttGGGGGCAGGGGGAGGTGCCATTCATTTAACATCATAATTCGCCTCTTATGATAATCTGATCACCTTGCCTTTGGCTAATGCTGGTTCAGTCTTCTTCTGATTGTTATTTGGGGGAGGCGGAGGGGGGCGGATAACTTTCGGAATAGcc                                                                                                                             <  4:1296427/149‑1 (MQ=255)

                            ttGGGGGCACTGGCTGTTGCCATGGATTTAACATAATAACGCCCCTGTGATCAATATCTTAACACCTTTCCATACCGTAATGCACGATCACTCATCATCTGTTGGTTTTTTGGGGGCGGCGGAGGGGGGCGGATAACTTTCGGAATAGcc                                                                                                                             <  1:888234/149‑1 (MQ=255)

                            aTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCg                                                                                                                            >  7:926492/1‑151 (MQ=255)

                               ggggCACCGGCAGGTGCCATTCATTAAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATCCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGAt                                                                                                                         <  7:520550/151‑1 (MQ=255)

                                     ccGCCAGGTGCCATTCATTTAAAAACATAATTCCCCTTTTATTAAAATCTGACCACCTTGCCATACCCTAATGCACCATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAt                                                                                                                   <  6:878200/151‑1 (MQ=255)

                                      cGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAtt                                                                                                                  >  2:1484183/1‑151 (MQ=255)

                                      cGGAAGGTGACATCAATTAAACATCATAATTCCCCTGTCATTAAAATCTCATCACCTTCCCATACCCTAAGCCAGCATCACTCAACATGTGAAGGATATTGGGGCGAGGCGGCCCGCGCCGGATAACTTTCGGAATAGCCGGATCAGGAtt                                                                                                                  <  1:404480/151‑1 (MQ=255)

                                       ggCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAttt                                                                                                                 >  6:407451/1‑151 (MQ=255)

                                       ggCAGGTGCCATGCATATAAAATCATAATTCCCCTGTGATTAAAATCTCATCCCCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGCCGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAttt                                                                                                                 <  5:1210106/151‑1 (MQ=255)

                                           ggTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcctc                                                                                                             >  6:1373076/1‑151 (MQ=255)

                                              gCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGCCGGATAACTTTCGGAATACCCGCATCAGCATTTcctcctc                                                                                                          >  4:240310/1‑150 (MQ=255)

                                               caattgaTTTAACATCAATAATCCGATCTTATGAAAATCTTCTCCCCTTCGCAATCCCTAACCCAGGATCACTCATCATGTGTAGGATATGTGGGGGAGCCGGACGCGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                          <  8:1465988/144‑1 (MQ=255)

                                                cattaaTTTAACATCATAATTCCCCTGTTATTAAAATCTGATCACCTTGCCATATCCTAATGCAGGATCACTCATCATCTGAATTATTTTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGaa                                                                                                        <  2:1961952/146‑1 (MQ=255)

                                                cATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGa                                                                                                         >  7:1690529/1‑150 (MQ=255)

                                                cATCCATTTAACATCAAACCTCCCCTGTGATCAAAATCTCACCACCTCCCCATAGCCTAATGCAGGATCACACATCATCTGAAGGATTTTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGaa                                                                                                        <  6:1108667/151‑1 (MQ=255)

                                                   tcATTTAACATCATAATTCCCCTGTGATGAAAATCTGAGCACCTTGCCATCGCCTAATGCAGCAGCACTCATCATCTGAAGGATTTTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAcc                                                                                                      <  3:36779/149‑1 (MQ=255)

                                                   gCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCTTAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGTAGGACGGGGGCGGAGAACTTTCGGAATAGCCGGATCAGGATTTCCTCGTGAACCg                                                                                                     >  6:379798/1‑151 (MQ=255)

                                                    cATTAAACATCATACATCCCCTGCCACCAACCTCTCCCCCCCTTCCCCTACCCTACTGCAGCATCACTCATCCTCTGCATGTTATGGGGGCGCCGCGGCCGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCg                                                                                                     <  2:366410/150‑1 (MQ=255)

                                                     aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                                   >  7:1618848/1‑151 (MQ=255)

                                                      attAACTTCATAATTCCTCTCTCAATAAAATCTTCTCACCTTTCCATACCCTAATGCATGCTCATATATCATCTCAACTTTTTTTGGGGGCGCAGCACGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCt                                                                                                  <  2:204745/150‑1 (MQ=255)

                                                        aaaaatcatAATTCCCCTGTGAGTAAAATCTGTTCACCTTCCCATAGCCTAATGCAGTATCACTCATCTTCTGATGTATTTTTGGGGTAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTa                                                                                                <  4:1096256/147‑1 (MQ=255)

                                                         aaCATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                               >  7:1962866/1‑151 (MQ=255)

                                                           catcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                               >  1:2011176/1‑149 (MQ=255)

                                                           catcatAAATCCCCTGTCAGCAAAATCTGATCACTTTCCCATAGCCTAATGCAGGATCACTCATCATCTGACGTATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAg                                                                                             <  6:760134/151‑1 (MQ=255)

                                                            atcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                               >  5:457335/1‑148 (MQ=255)

                                                              catACTTCCCCTGTGAGGAAAATCGGAGCCCCTTGCCAGAGCCTACGGCCGGATCACTCCTCCTCGGCCGGCCTTGGGGGCGACCCCGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                            <  7:1830351/149‑1 (MQ=255)

                                                              aaaaaatCCCCTTTCATTAAAATCTTATCACCTTGCCATATCCTAATGCACGATCCCTCATCATCTCAAGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc                                                                                          <  2:467618/145‑1 (MQ=255)

                                                                 aaTTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                        >  8:561214/1‑150 (MQ=255)

                                                                     ccccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGCACGCAAgg                                                                                    >  6:1557377/1‑150 (MQ=255)

                                                                      cccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACTCAAGGAt                                                                                  >  7:1744661/1‑151 (MQ=255)

                                                                           tgatgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATTTTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACt                                                                              <  2:668324/150‑1 (MQ=255)

                                                                            gaagaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTgg                                                                            >  8:826311/4‑151 (MQ=255)

                                                                                 aaaTCTGCTCACCTTGCCATAGCTTTATGCGGGATCACTCTTGATCTGATGGATATTTGGGGGTGGCGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                       <  2:1707004/151‑1 (MQ=255)

                                                                                      tGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                   >  5:839254/1‑150 (MQ=255)

                                                                                       gATCCCCTTGCCATAGCCTACTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccag                                                                 <  5:104614/151‑1 (MQ=255)

                                                                                       gATCCCCCTGCCATACCCTAATGCAGGATCACTCATCATCTGACGGATATTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccag                                                                 <  7:598962/151‑1 (MQ=255)

                                                                                       gATCACCTTGCCATAGCCTAATGCAGTATCACTCATCATGTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                     <  2:76055/147‑1 (MQ=255)

                                                                                       gATCACCTTCCCATAGCCTAATGCAGCATCACTCATCATCTGAAGGATTTGTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagc                                                                    <  4:391068/148‑1 (MQ=255)

                                                                                         tCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTAAGCCAGCt                                                               >  7:1217043/1‑151 (MQ=255)

                                                                                           aCCTTGCCATCGCCTACTCCAGGATCACTCATCATCTTAAGTATATTTGGGGGCGGTGGCCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGa                                                             <  8:1180358/151‑1 (MQ=255)

                                                                                            gacacccAGAGCCTAAGGCCGCATGACACATCCTCTGATGGATTGGTGGGCGAGGAGCCCCCGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGAc                                                            <  3:950894/146‑1 (MQ=255)

                                                                                               tGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGAt                                                         >  3:1355759/1‑151 (MQ=255)

                                                                                                gcaaTAGCCTCTTGCAGGATCCCTCAACATCTGAAGTCTATTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATc                                                        <  3:1918149/148‑1 (MQ=255)

                                                                                                  cATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGt                                                      >  4:835150/1‑151 (MQ=255)

                                                                                                   aTAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTg                                                     >  3:120286/1‑151 (MQ=255)

                                                                                                   aTAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGACAGCTGACGATCGTg                                                     >  1:1597602/1‑151 (MQ=255)

                                                                                                    tAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTgg                                                    >  4:1429508/1‑151 (MQ=255)

                                                                                                     aGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTAAGCCAGCTGACGATCGTggc                                                   >  8:1573321/1‑151 (MQ=255)

                                                                                                      cccTAATGCAGGATCACTCCTCCTCTGGCGGCTCGGGGGCGGCGGCGGCCGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcg                                                  <  7:760688/150‑1 (MQ=255)

                                                                                                       ccTAATGCCCGAGCACTCATCATCTGAAGGATTTTTGGGCGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACTATCGTggcgg                                                 <  2:1156009/151‑1 (MQ=255)

                                                                                                       ccTAATGCAGGATCACTCATCCTCTGAAGGATCTGTGGGGGCGGCGGCCCGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcgg                                                 <  3:637056/151‑1 (MQ=255)

                                                                                                       ccTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcgg                                                 >  4:1972838/1‑151 (MQ=255)

                                                                                                        cTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                                >  1:695817/1‑151 (MQ=255)

                                                                                                         tAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGgcggcg                                               >  5:328733/1‑151 (MQ=255)

                                                                                                          aagcccggATCACTCCTCATCTGAAGGATATTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGgcggcg                                               <  4:488707/144‑1 (MQ=255)

                                                                                                          aaTGCATGTTCTGTCATCCTCTGACTGATATTTGGGGTTCGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGgcggcg                                               <  6:1887107/150‑1 (MQ=255)

                                                                                                           aTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGtt                                             >  4:186382/1‑151 (MQ=255)

                                                                                                              cAGGAGCCCTCATCATCTCATCGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaa                                          <  5:1757627/151‑1 (MQ=255)

                                                                                                               aGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTACTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaa                                          >  3:1756772/1‑150 (MQ=255)

                                                                                                               aGGATCACTAATCCTCGGACGGCTATTTGGGCGAGCAGGACGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaaa                                         <  5:1940870/151‑1 (MQ=255)

                                                                                                                 ccacACTCATCATCTGAAGCAAATCAGCCGCACGACGCCGGCGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACa                                       <  3:1252792/148‑1 (MQ=255)

                                                                                                                   tCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATc                                     >  4:1671258/1‑151 (MQ=255)

                                                                                                                     aCTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc                                    >  8:1503511/1‑150 (MQ=255)

                                                                                                                       tcatcaTCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAATCATCCAGc                                 >  3:1878007/1‑151 (MQ=255)

                                                                                                                       tcatcaTCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGc                                 >  8:1373312/1‑151 (MQ=255)

                                                                                                                       tcatcaTCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGc                                 >  5:91589/1‑151 (MQ=255)

                                                                                                                       tcatcaTCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGCATTTCCTCCTGAACAGGCTTACAGAACGCAACGATTACTGCTTTTCAGCCAGATGACGATCGTGGCGGCGTAAAAACATCCAGc                                 >  2:1371184/1‑151 (MQ=255)

                                                                                                                        catcatGTTAAGTATTTTGGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                                <  4:693257/151‑1 (MQ=255)

                                                                                                                         atcatcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                                >  8:284284/1‑150 (MQ=255)

                                                                                                                          ccatcTGCCGGATATTGGGGGGCGGCGGCCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCaa                              <  8:1055153/150‑1 (MQ=255)

                                                                                                                           catcTGAAGGATATGTGGGGGCGGAGGACGGCGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAAt                             <  3:1563868/151‑1 (MQ=255)

                                                                                                                           aatcTGAAGGATATTTGGGGGCCGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAAt                             <  2:1568496/150‑1 (MQ=255)

                                                                                                                            atcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                            >  1:1764643/1‑151 (MQ=255)

                                                                                                                                gAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                         <  6:1723482/150‑1 (MQ=255)

                                                                                                                                gAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                         >  5:155492/1‑150 (MQ=255)

                                                                                                                                 aaGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAg                       <  1:148371/151‑1 (MQ=255)

                                                                                                                                 aaGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                         >  8:1846523/1‑149 (MQ=255)

                                                                                                                                    gaaatTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                    <  1:103356/148‑1 (MQ=255)

                                                                                                                                     atatTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                    >  5:572923/1‑150 (MQ=255)

                                                                                                                                     atatTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTCGTATTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                    >  8:1953846/1‑150 (MQ=255)

                                                                                                                                      tatTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcaca                   >  1:1228501/1‑150 (MQ=255)

                                                                                                                                       ttttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaa                  <  8:887257/149‑1 (MQ=255)

                                                                                                                                        tttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaac                 >  7:1323743/1‑150 (MQ=255)

                                                                                                                                         ttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca                >  7:370634/1‑150 (MQ=255)

                                                                                                                                         ttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca                >  3:512/1‑150 (MQ=255)

                                                                                                                                          tGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCAcaacaa               <  2:974398/150‑1 (MQ=255)

                                                                                                                                               gaggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcg         <  2:827226/151‑1 (MQ=255)

                                                                                                                                                    ggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGaa     >  6:537368/1‑150 (MQ=255)

                                                                                                                                                    ggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGaa     >  3:769863/1‑150 (MQ=255)

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`