Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA26.1% 362.7 / 25.2 262intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (146/27);  new base A (61/0);  total (234/28)
Fisher's exact test for biased strand distribution p-value = 2.63e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

GCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613621‑613919
                                                                                                                                                     |                                                                                                                                                      
    taCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGGAGAAAGATGAAGGGCTGTCCACGTGACGATCCGCAAAAGACAGCGGCTTCTCGGTCAGTTTCACGTg                                                                                                                                                   >  7:1515935/1‑151 (MQ=11)
      cccAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATTCGGCGGGTTTTTGATTTTGGAGGCGCAGAAAGAAGAATGACTGTCAAAGACACTATACCCAAAAGAAACACGCTGATGGGTCAGTTTCaaatca                                                                                                                                                  >  6:1708499/1‑145 (MQ=11)
         aaaaGAAAGCGGCTTATCGGTCAGTTTGACCTGGTTTACGTAAAAACCCGCTGCGGCGGGTTTATGCTTTTTGAGTGCGCGAAAGATCAGACCCATTCCGCGAGACAAGACTAAAAAGAATGCGTCTTAACGGACAGTGTAAACTGtgtt                                                                                                                                               >  1:1071847/1‑150 (MQ=2)
             gaaagCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAACCGGTGCGGCGGGTTTTTGCTTTTTGAGTGGGAGATAGATTAATAACAGGACACGACTCAAGACCCGTAAGACAGCGGCTTATCGTTCCGGTTCAGCTGATTTaatt                                                                                                                                           >  5:35677/1‑147 (MQ=2)
                  ccgagcttCGGTCAGTGGCACCTGGTTAACGTAAAACCCCGCTGCGGGTGTTTTTTGCTTTTCGAGGGGAACAAAGATCAATCCCGGTCCACGAAACTATACCCAAAAGAAAGCGGATTATCGGTCAGTTTCACCTGTTTTACGTAAAAAc                                                                                                                                     <  5:513781/144‑1 (MQ=9)
                    gCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGATTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAACATGAATAACTGGCCACGACAGTATACCCAAAACAAAGCCGATTATCGTTCGCTTTCATCTGATTTACGTAAAAAc‑cc                                                                                                                                  >  5:829431/1‑151 (MQ=11)
                     cTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGATACGGCGGGTTTTTGATTTTTGAGGGCCAGAAAGAAGAATGACAGTCCACGACACTATACCCAAAAGAAAGCGGCTAATCCATCAGTTTAAACTGATGTACGTAAAAAc‑cac                                                                                                                                 >  8:255145/1‑149 (MQ=11)
                         tCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTaaaaaacagatac                                                                                                                              >  4:883508/1‑143 (MQ=255)
                         tCGGTCAGTTTCACCTGGTTTACGTAAAAACACGATTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTACACGACACTATACCCAAAATAAAGAGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAc                                                                                                                                     >  6:513240/1‑144 (MQ=255)
                          cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTGTACGTAAAAAA‑CCGCTTcg                                                                                                                            >  3:1005160/1‑151 (MQ=255)
                           ggTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTGTACGTAAAAAC‑CCGTTTcgg                                                                                                                           >  5:1060411/1‑151 (MQ=255)
                           ggTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGtaataaacagatact                                                                                                                             >  6:322783/1‑141 (MQ=11)
                               aGTTTCACCTGGTTTAAGTAAAAACCCGGTTCGGCGGGTTTTTGCTTTTCGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCAAAAAGAAAGCGGCTTATCGCTCAGTTTGACCTCTTTAACGTAATAAA‑CCCCTCCGGCggg                                                                                                                       >  3:788825/1‑151 (MQ=11)
                                  ttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAAC‑CCGGTGCGGCGGGttt                                                                                                                    >  3:1546841/1‑151 (MQ=255)
                                  ttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTGTACGTAAAAAC‑CCGGTCCGGCGGGttt                                                                                                                    >  4:1449008/1‑151 (MQ=255)
                                   tCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTGTACGTAAAAAA‑CCGATACGGCGGGtttt                                                                                                                   >  4:249983/1‑151 (MQ=255)
                                   tCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACATGTTTTACGTAAAAAC‑CCGTTACGGCGGGtttt                                                                                                                   >  4:379876/1‑151 (MQ=255)
                                    cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTGCGGCGGGtttc                                                                                                                   >  5:1223909/1‑149 (MQ=255)
                                     aCCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTCCTTTTGGAGGGGCAGAAAGATGAATGACTGTCAACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAC‑CCGATACGGCGGGttaaa                                                                                                                  >  2:790113/1‑147 (MQ=11)
                                      ccTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAA‑CCGATACGGCGGGtttg                                                                                                                   >  2:1276002/1‑147 (MQ=11)
                                       cTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAAA‑CCGGTGCGGCGGGTTTTTGCt                                                                                                               >  3:1978707/1‑151 (MQ=11)
                                       cTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATGTACGTAAAAAC‑CCGCATcggatgcgctgtcgt                                                                                                               >  3:1467391/1‑139 (MQ=11)
                                        tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTGCGGCGGGTTTTTGCtt                                                                                                              >  7:1156797/1‑151 (MQ=18)
                                        tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAA‑CCGCTTCGGCGGGTTTGTGCtt                                                                                                              >  3:242454/1‑151 (MQ=16)
                                        tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAA‑CCGATACGGCGGGttttaaatt                                                                                                              >  6:806284/1‑146 (MQ=11)
                                        cccccccccacaaccccccCTCCGCCGGTTTTTTGC‑TTTGCAGGGCCCGAAACATCAATCACGCGCCACGACACTATACCCAACAGCACCCCGCGTATCCGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACttt                                                                                                             <  5:1324546/136‑1 (MQ=2)
                                         ggTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACATGTTTTACGTAAAAAA‑CCGCTTCGGCGGGtttgtgattt                                                                                                             >  4:1595716/1‑144 (MQ=255)
                                           tttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAA‑CCGATACGGCGGGtttaaaattttt                                                                                                           >  4:1872986/1‑142 (MQ=11)
                                            ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAA‑CCGCTGCGGCGGGtttgagatttttg                                                                                                          >  2:539081/1‑141 (MQ=11)
                                            ccacctaaacccccGCTGCGGCGGGTTTTTGCTTTTGGAGGTGATTTATTATGATTGTGTGTCCACGACTCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCTCCTTTCTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTgg                                                                                                          <  8:1961077/140‑1 (MQ=2)
                                              aCGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAC‑CCGGTGCGGCGGGTTTGTGCTTTTgg                                                                                                          >  1:506043/1‑149 (MQ=255)
                                               cGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTCATTTACGTAAAAAC‑CCGCTACGCCGGGGTTAAAATTTTGGAgg                                                                                                       >  6:162353/1‑151 (MQ=11)
                                                gTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAAC‑CCGGTTCGGCGGGTTTGTGCTTGTGGAggg                                                                                                      >  1:207917/1‑151 (MQ=11)
                                                gTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAC‑CCGCTGCGGCGGGTTTGTCCTTGTGGAggg                                                                                                      >  7:353582/1‑151 (MQ=255)
                                                gTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAA‑CCGATACGGCGGGTTTATAATTTTTGAgtg                                                                                                      >  8:1323391/1‑149 (MQ=11)
                                                 tAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAC‑CCGGTGCGGCGGGTTTGTCCTTGTGGAgggg                                                                                                     >  5:1506728/1‑151 (MQ=11)
                                                          cctgtttcggTTTATTCCTTTTTGCGGAGCTGAAAGATGAATGACTGTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGat                                                                                            <  6:1084985/144‑1 (MQ=11)
                                                             tCGGCGGGTTTTTGCTTTTGGAGGGGCACAAAGATGAATGCCTGTCCACGACACTATAAACAAAAGAAAGCGGCTGATGGGTCAGATTCACATGTTTAAAGTAAAAAC‑CCGCTTCGGCGGGTTGTTGGTGTTGGAGGGGctgggagggggg                                                                                         >  6:1402694/1‑140 (MQ=11)
                                                              cggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACCCTATACCCAAAAGAAAGCGGCTTATAGCTGAATATCACCCGTGTTAACTAAAAACACCGC‑TCCTTGGGTGTTTTGGGGTGGAGGGGAAGAAAAatgaat                                                                                        >  7:781795/1‑151 (MQ=2)
                                                              cggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAC‑CCGCTGCGGCGGGTTTGTGATTTTGTCGGGGGAGAACGatgatt                                                                                        >  1:902002/1‑149 (MQ=11)
                                                              cggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAC‑CCGCTACGGCGGGTTTGTGATTTTTGAGTGGCAGatataggaa                                                                                         >  8:391500/1‑142 (MQ=11)
                                                                 cggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACCCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCAACTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTAGATCGGCAGagcgtggagtagg                                                                                     >  6:1655302/1‑139 (MQ=11)
                                                                  gggTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAAC‑CCGATGCGGCGGGTTTAAACTTTTTGAGTGCCAGaaaagataataagt                                                                                    >  8:515135/1‑140 (MQ=11)
                                                                   ggTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATGTACGTAATAAC‑CCGATACGGCGGGTTTAAACTTGTGGAGTGCCATAAAgaagaaacacat                                                                                   >  6:604910/1‑145 (MQ=1)
                                                                    ggttctGCTTTTGGCGGGGCCGAAAGTTCATTCCCTGTCCACTACTTTATACGAAAACGATAGCTGCTTTTCGTTCAGTTTCTCCTCTTTTACTTAAATAC‑ACGATTCTGTTGGTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGt                                                                                  <  8:717861/146‑1 (MQ=2)
                                                                           cTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAC‑CCGCTGCGGCGGGTTTGTAATTTTTGAGGGGCAGAAAGAGAAATGACTGTCCACGac                                                                           >  7:150189/1‑151 (MQ=11)
                                                                                       cAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAC‑CCGATACGGCGGGTTTGTACTTTTTGAGTGGCAGAAAGATAAATAACTGTACACGAAAGTATACCCaa                                                                >  8:116567/1‑150 (MQ=11)
                                                                                         gAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAC‑CCGATTCGGCGGGTTTTTACTTTTTGAGTGGCAGTTAGATGTAACCCTTTCCACGAGACtaaaatctaatg                                                             >  8:1942139/1‑141 (MQ=11)
                                                                                                    gaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGCCAGAAATAAGTATCACTTTCCACGACACTAGAGCAAAAAGAAAGcccatt                                                   >  3:538102/1‑145 (MQ=11)
                                                                                                           cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAC‑CCGGTTCGGCGGGTTTATCATTTTTGAGGGGCATAAATATGAAACACTTTCGACGACAAGATAGAAAAAAGAAAGcaaaagaacgcta                                            >  6:1383586/1‑138 (MQ=2)
                                                                                                             cGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTAATTTTGGAGGGCCATAAATAAGATGGTCTTGCCCCGACACTATCAGCTAAAGAATCCCCCTTATCggaaagt                                         >  7:1997396/1‑146 (MQ=2)
                                                                                                                cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGGTTCGGCGGGTTTTTACTTTTGGAGTGGCAGATAGATCAATCACTGTCCCCGACACTATAGACAAAAGAAACCGGCTTATCGGTAAGtttt                                      >  1:361257/1‑151 (MQ=11)
                                                                                                                cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGATGAATGACTTTCGACGACACTATACCAAAAAGAAAGCGGCTTATCGGTCAGtttt                                      >  1:2011862/1‑151 (MQ=255)
                                                                                                                 acTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGATTCGGCGGGTTTGTACTTTTGGAGTGCGAGATAGAAGAAACACTTTCCCCGACACTTAACCAAAAAGAAAGCGGCTTATCGCTCAGtttt                                      >  1:1355992/1‑150 (MQ=11)
                                                                                                                 acTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAA‑CCGGTTCGGCGGGTTTGTAATTTTTGAGTGGCAGAAAGATGAAAGACTGTCCACGACACTATCAGCTAATGAAAGCGGCTTATCgctaattgtt                                      >  6:1150631/1‑141 (MQ=11)
                                                                                                                      aCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAAGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGCTCAGTTTTACCTg                                 >  2:1575486/1‑150 (MQ=25)
                                                                                                                       cccAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATGAt                               >  8:1732744/1‑151 (MQ=37)
                                                                                                                        cctaaagaaagCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAC‑CCGCTCCGGCGGGTTTAAACTTTTGGAGTGGCATAAAGTTGAATTACTGTCAACCCCACTATGCCCAAAAGAAACCGGGTTATCGGTCAGtttaccacgagt                              >  1:1007998/4‑142 (MQ=11)
                                                                                                                          aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGCCAGATATAAGAATGACTGTCCACGACACTATACACAAAAGAAACCCCCTTATCGGTCAGTTATACCTGATgac                            >  7:1549734/1‑149 (MQ=14)
                                                                                                                            cagaaacCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACt                                                                                                               <  4:1036413/65‑1 (MQ=255)
                                                                                                                             agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTGCACGACACTATACACAAAAGAAAGCGGCTGATCGGTCAGTTTTACGAGATGTACGt                         >  8:425738/1‑151 (MQ=255)
                                                                                                                             agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATCAATCACTGTCCACGACACGATAACAAAAAGACAACGGCTTATCGGTCAGTTTTACCTAATCTActt                         >  8:1878548/1‑149 (MQ=11)
                                                                                                                              gaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGt                         >  1:129114/1‑150 (MQ=40)
                                                                                                                               aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCATAAAGATGAAAGACTGTCCACGAGACTATAGGCAAAAGAAAGAGGCGTAGCGGTAAGGTTTACCTGATGTACGtat                       >  2:1765629/1‑150 (MQ=14)
                                                                                                                               aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCAAAAAGAAAGCGGCTTATCGGTCAGTTATACCTGATGTACGta                        >  1:868343/1‑150 (MQ=255)
                                                                                                                                aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAATATGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTGATCGGTCAGTTTTAAGTGATGTACGtaat                      >  2:123296/1‑151 (MQ=14)
                                                                                                                                aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGACGGGCAGAAAGATGAATGACTGTCCACGACACTATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATGATGTACGtaat                      >  7:1431498/1‑151 (MQ=255)
                                                                                                                                 agCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAAAGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                      >  4:533003/1‑150 (MQ=255)
                                                                                                                                   cgccTTATCGGTCAGTTTCACCTGATTTACGTAAAAAC‑CCCCTTCGGCGGGTTTAAACTTTTGGAGGGCCATAAAGATAAATCACAGTCCACGACACAATAACCAAAAGAAAACGGCTTAACGCTCAGTTTTAACAGATGTaataaataa                    >  4:1687165/4‑142 (MQ=2)
                                                                                                                                   cGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaataa                    >  2:1639689/1‑150 (MQ=255)
                                                                                                                                   cGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAGGAATGCCTGTCCACGACACGAGCCCCAAAAGACACCGGCTTATCGGTCAGTGTTACCAGATGAACGaaataa                    >  3:16879/1‑150 (MQ=14)
                                                                                                                                   cGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAC‑CCGGTGCGGCGGGTTTTTACTTTTTGAGGGCCAGATATAAGAAAGACTTTCCACGACACTATCAGAAAAAGCAAGCGACTTATCGGTCAGTAATAAATTATGAACGtaataa                    >  6:2069446/1‑150 (MQ=255)
                                                                                                                                    ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGGGGTTTTTTAGTTTTGGCGGGGAACAAATATCAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  <  7:913659/151‑1 (MQ=14)
                                                                                                                                    ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  >  3:178993/1‑151 (MQ=255)
                                                                                                                                    ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  >  1:1603220/1‑151 (MQ=255)
                                                                                                                                    ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAACTGATGTACGTAATAAAc                  >  4:958318/1‑151 (MQ=255)
                                                                                                                                     gCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTTTCCACGACACTATAACCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATGTACGTAATAAAcc                 >  2:592631/1‑151 (MQ=11)
                                                                                                                                      cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  5:679824/1‑151 (MQ=255)
                                                                                                                                      cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  7:991711/1‑151 (MQ=255)
                                                                                                                                       ttATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                 >  3:1732872/1‑149 (MQ=255)
                                                                                                                                        tATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt              >  7:1900958/1‑151 (MQ=255)
                                                                                                                                        tATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt              >  5:1867242/1‑151 (MQ=255)
                                                                                                                                          tCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc            >  1:796802/1‑151 (MQ=255)
                                                                                                                                          tCGGTCAGTTTCACCTGGTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGTTGTACGTAATAAACCgctgc            >  7:1533298/1‑147 (MQ=2)
                                                                                                                                            ggTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg          >  2:1966595/1‑151 (MQ=255)
                                                                                                                                            ggTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg          >  3:324333/1‑151 (MQ=255)
                                                                                                                                            ggTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg          >  3:1049148/1‑151 (MQ=255)
                                                                                                                                             ntCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTNGGAGGGGCAGAAAGATGNATGACTGTNCNCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc         >  8:1032019/2‑151 (MQ=255)
                                                                                                                                              tCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGCCAGAAAGATGAATGACTCTCCACGACACAATACCCAAAAGAAAGCGGCTTATCGGTCAGATATACCAGATGTACGTAATAAACAGTTCcggcg        >  4:1675238/1‑151 (MQ=17)
                                                                                                                                               cAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       >  5:203679/1‑151 (MQ=255)
                                                                                                                                               cAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       >  2:321010/1‑151 (MQ=255)
                                                                                                                                                aGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg      >  2:1506048/1‑151 (MQ=255)
                                                                                                                                                aGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg      >  3:548752/1‑151 (MQ=255)
                                                                                                                                                aGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATAGGTCAGTTTTACCTGATTTACGTAATAAACCGTTCcggcggt      >  8:2051498/1‑150 (MQ=255)
                                                                                                                                                  tttCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  8:278172/1‑151 (MQ=255)
                                                                                                                                                  tttCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  7:1319561/1‑151 (MQ=255)
                                                                                                                                                  tttCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  6:833744/1‑151 (MQ=255)
                                                                                                                                                    tCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  3:44727/1‑151 (MQ=255)
                                                                                                                                                     cACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  3:1281767/1‑150 (MQ=255)
                                                                                                                                                     cACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  7:571602/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAC‑CCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613621‑613919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑