Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.27.2% 388.8 / 153.0 156intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (100/10);  new base . (39/2);  total (143/13)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.75e-02

Only 100 of 162 total aligned reads displayed.

CACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936148‑936443
                                                                                                                                                   |                                                                                                                                                      
cACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTAATGCTATACTTCATCAAAatttt                                                                                                                                                    >  3:330506/1‑149 (MQ=255)
                              atcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGCGGAGGGGGTGAATACATCTCAATTTAATTGTTTGCGGGGGCATGATTAGACCctacg                                                                                                                     >  7:1939235/1‑151 (MQ=11)
                                    tttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTACTGCGTTACTTCATCGAATTTTAATTGGTTGCGGGGGCCGGATTTCCCCCTACGACCttg                                                                                                               >  7:1153432/1‑150 (MQ=14)
                                         ttGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGtt                                                                                                                                             <  3:1435032/116‑1 (MQ=255)
                                                cGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCAACAAAATTTAATTGGtt                                                                                                                                             >  8:531136/1‑109 (MQ=255)
                                                        ggATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCGCGGTAGTGCATCTCTTCAACAAAATTTAATTGGTTGCGGCTGGGTGTTTTGCACAGACCACATTAGGGTTAttatacagaca                                                                                              >  8:1114092/1‑138 (MQ=2)
                                                             tGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGCCGACCTTCGGGTTATGAGCCCGACGAGCTAca                                                                                       >  7:801166/1‑149 (MQ=255)
                                                              gAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttt                                                                                                                                                      >  7:1990192/1‑86 (MQ=35)
                                                                         cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTCAGGGGGAAGTTTATGACCAGACGACCTTGGGCTTATTAGGCAGAACAGCTaaagccttatccacac                                                                          >  7:354976/1‑136 (MQ=9)
                                                                                       cccgtagaGCTCCGAGGCTCCTGCACCCCCCGTCACAGTACTGCTGGCTTTGGTGGTTTATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAt                                                            <  7:1497103/145‑1 (MQ=11)
                                                                                          gacgaGCTACCAGGCTGCTCCACCCCGCGTCACCGTAGTCGTTTACTTCATCAAATTTTTTTTGGTTGCGGGGGCGGGATTTGAACCGCCGACATTCGGGTTATGAGACCGCCGAGCTACCAGCCTCCTCCACACCGCTTCAGTGGATgcg                                                         >  7:1437658/1‑151 (MQ=14)
                                                                                            cgaGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATgcgcc                                                       >  7:812995/1‑150 (MQ=255)
                                                                                                        ctgctCCACCCCGCGTCACCGTAGTGCTTTACTTCATCTAATTTTATTTGGTTGCGGGGGGCGGATTTGCACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCGCCCGCGTCCGTGGATGCGCAGTATACTCCGcg                                           >  4:1308673/1‑150 (MQ=17)
                                                                                                            tCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                       >  5:6420/1‑151 (MQ=255)
                                                                                                             ccACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                      >  2:1268793/1‑151 (MQ=255)
                                                                                                             ccACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGCTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCGCTCGCGt                                      >  6:1604555/1‑151 (MQ=255)
                                                                                                              cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                     >  8:485419/1‑151 (MQ=255)
                                                                                                              cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                     >  6:357338/1‑151 (MQ=255)
                                                                                                              cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCAGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                     >  1:88580/1‑151 (MQ=255)
                                                                                                                ccccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCAGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAAGCGCACAATACTCCCCTCGCGtatt                                   >  1:1766049/1‑151 (MQ=11)
                                                                                                                 cccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATTCGCACTATACTCCGCTAGAGTATTc                                  >  2:1173123/1‑151 (MQ=11)
                                                                                                                 cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  8:2017309/1‑151 (MQ=255)
                                                                                                                 cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  3:516172/1‑151 (MQ=255)
                                                                                                                  ccgtgttacgttattGCTTTTCTTTCTCAAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCa                                 <  5:777279/137‑1 (MQ=17)
                                                                                                                  ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  1:447578/1‑150 (MQ=255)
                                                                                                                  ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  5:105385/1‑150 (MQ=255)
                                                                                                                  ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  7:1328439/1‑150 (MQ=255)
                                                                                                                  ccGCGTCACCGTACTGCTTTAATTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  4:1481750/1‑150 (MQ=255)
                                                                                                                    gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                               >  3:1603990/1‑151 (MQ=255)
                                                                                                                     cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  1:1061018/1‑151 (MQ=255)
                                                                                                                       tCACCGTACTGCTTTTACTTCTTAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgc                                        <  6:592575/139‑1 (MQ=17)
                                                                                                                       tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  2:1832348/1‑150 (MQ=255)
                                                                                                                        cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                    <  4:1656924/142‑1 (MQ=255)
                                                                                                                        cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                           >  2:629439/1‑151 (MQ=255)
                                                                                                                         aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCTTTTTCAt                                >  6:1333234/1‑145 (MQ=14)
                                                                                                                         aCCGTACTGCTTTACGTGCAGCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                                <  5:1334412/145‑1 (MQ=17)
                                                                                                                         aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                          >  8:115135/1‑151 (MQ=255)
                                                                                                                         aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                          >  4:969800/1‑151 (MQ=255)
                                                                                                                          ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCGCAATATACTCCGAAGGAGTTttcattcacgct                         >  7:582128/1‑147 (MQ=11)
                                                                                                                           cGTACTGTTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                        >  7:1886291/1‑151 (MQ=255)
                                                                                                                           cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                        >  1:705072/1‑151 (MQ=255)
                                                                                                                           cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                        >  8:340692/1‑151 (MQ=255)
                                                                                                                            gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGAGGAGGCGGTCTATACTACGCTAGAGTTTTCATTCAACCttt                       >  8:1727907/1‑151 (MQ=11)
                                                                                                                            gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                       >  8:210337/1‑151 (MQ=255)
                                                                                                                            gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                       >  4:837279/1‑151 (MQ=255)
                                                                                                                             tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTAGGCCAAATATACTACCCTAGAGATTTCGTGCAACCttat                      >  6:2032153/1‑149 (MQ=11)
                                                                                                                             tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                      >  1:1463256/1‑151 (MQ=255)
                                                                                                                              aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGAGTTTTCATGCAACCttttt                     >  5:1950927/1‑151 (MQ=17)
                                                                                                                              aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                     >  1:27139/1‑151 (MQ=255)
                                                                                                                               cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGCGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    >  8:231156/1‑151 (MQ=255)
                                                                                                                               cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    >  4:1116216/1‑151 (MQ=255)
                                                                                                                                tgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGCTTTTTCATTCAACCttttttt                   >  5:1180283/5‑151 (MQ=17)
                                                                                                                                tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  4:362290/1‑151 (MQ=255)
                                                                                                                                tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAAACGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  3:1824924/1‑151 (MQ=255)
                                                                                                                                 gcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAGTCGCACTATACAGCGATATAGTTTTGATTCACCCttgttttt                  >  7:433931/4‑151 (MQ=11)
                                                                                                                                  cttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTCCGCTCGCGttt                                    >  7:1913272/3‑132 (MQ=255)
                                                                                                                                  cttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGATCCACCCCGCGTCCGTGGATTCGGAGTATACTGCGATCGAGTTTTCATGCaggcgttatttt                  >  6:1275950/3‑139 (MQ=11)
                                                                                                                                  cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                  >  2:1506196/1‑150 (MQ=255)
                                                                                                                                  cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                  >  3:1152236/1‑150 (MQ=255)
                                                                                                                                   ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCGGGTTTTTAc                >  1:1104566/2‑151 (MQ=17)
                                                                                                                                    tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                >  7:616687/1‑151 (MQ=255)
                                                                                                                                    tttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGTAATCGAAGAATACTACTATATATTTTTCATGCAACGTTTATTTTagc               >  4:229894/1‑149 (MQ=11)
                                                                                                                                     ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc               >  8:608873/1‑151 (MQ=255)
                                                                                                                                     ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc               >  3:78052/1‑151 (MQ=255)
                                                                                                                                     ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc               >  7:1513589/1‑151 (MQ=255)
                                                                                                                                     ttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCTGGTTTTCATGCACCCTTGTTTTTAccc              >  5:250545/1‑151 (MQ=17)
                                                                                                                                      tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCAGACGAGCTACCAGGCTGCTCCACCCCGCGTCCTTGGATGCGCACTATACTCAGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              >  2:313264/1‑151 (MQ=255)
                                                                                                                                      tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGAGTTTTCATGCAACGtttt                      >  5:288433/1‑142 (MQ=255)
                                                                                                                                      tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGCACTATACTcccatggcgta                                     >  3:1150120/1‑118 (MQ=14)
                                                                                                                                      tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCTCTATACTCCGATCGCGTTTTCATGCCCCCGTGTTTTTAcgcc             >  3:1441885/1‑151 (MQ=14)
                                                                                                                                      tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGATGCGCACTATACTCCGCTCGAGTTTTCATTCAACCTTAATTTTAaccc             >  6:1016305/1‑151 (MQ=17)
                                                                                                                                       aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACAGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc             >  1:1429620/1‑151 (MQ=255)
                                                                                                                                       aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTAAGCGATAAATACTACACTCTATATTTCATGCAGCCTTATTTTTAacccc            >  2:1328466/1‑150 (MQ=11)
                                                                                                                                       aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCAGAATACTACTCTCGAGTTTTCATGCCACCTTGTTTTTaacacc            >  6:536743/1‑146 (MQ=14)
                                                                                                                                        cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCCCTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccccc            >  1:1875721/1‑150 (MQ=255)
                                                                                                                                        cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTAAACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTaacacc            >  8:53546/1‑146 (MQ=255)
                                                                                                                                        cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGAGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              >  7:281249/1‑149 (MQ=255)
                                                                                                                                          ttCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCTTGTTTTTACCCCGAt          >  6:527168/1‑151 (MQ=255)
                                                                                                                                          ttCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGGTCAATACTCCTCTCTCTTTTTGCTTCAGGCGTTATTTTTCCCCGAt          >  5:1366425/1‑151 (MQ=11)
                                                                                                                                           tcattaAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGa           <  6:132708/150‑1 (MQ=255)
                                                                                                                                           tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  1:996521/1‑151 (MQ=255)
                                                                                                                                           tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  4:195521/1‑151 (MQ=255)
                                                                                                                                           tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  6:764957/1‑151 (MQ=255)
                                                                                                                                           tcatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCAATATAGATTTCATGCAACCTTTATTTTAACCCGAt          >  4:1492796/1‑150 (MQ=17)
                                                                                                                                            catcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGAAGAATACTACACTCTAGTATTCATTCAACCTTATTTTTagcaccatc         >  6:1851632/1‑142 (MQ=14)
                                                                                                                                             atcaAATTTTAATTTGTTGCGGGCGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTACGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCTATc         >  7:718779/1‑150 (MQ=255)
                                                                                                                                              tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc       >  4:1415683/1‑151 (MQ=255)
                                                                                                                                              tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc       >  6:1500395/1‑151 (MQ=255)
                                                                                                                                              ataaaTTTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc       <  5:1307112/149‑1 (MQ=255)
                                                                                                                                               caAATTTTAATTGGTTGCGGGGGCCGGATTTGTACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg      >  1:825435/1‑151 (MQ=255)
                                                                                                                                               caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg      >  7:1281742/1‑151 (MQ=255)
                                                                                                                                               caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg      >  5:1666989/1‑151 (MQ=255)
                                                                                                                                                 aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  7:1166011/1‑150 (MQ=255)
                                                                                                                                                 aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  7:424105/1‑150 (MQ=255)
                                                                                                                                                 aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     >  7:527046/1‑150 (MQ=255)
                                                                                                                                                  aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt   >  7:1057169/1‑151 (MQ=255)
                                                                                                                                                  aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt   >  1:1694418/1‑151 (MQ=255)
                                                                                                                                                   ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt  >  5:90630/1‑151 (MQ=255)
                                                                                                                                                   ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt  >  6:2024798/1‑151 (MQ=255)
                                                                                                                                                   ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt  >  1:1325352/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936148‑936443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: