Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | DH1 | 1,016,179 | T→A | intergenic (+30/‑33) | nlpD → / → rpoS | murein hydrolase activator NlpD/RNA polymerase sigma factor RpoS |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | DH1 | 1,016,179 | 0 | T | A | 95.3% | 1926.5 / 54.2 | 551 | intergenic (+30/‑33) | nlpD/rpoS | murein hydrolase activator NlpD/RNA polymerase sigma factor RpoS |
Reads supporting (aligned to +/- strand): ref base T (11/15); new base A (255/269); total (267/284) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.53e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01 |
Only 100 of 552 total aligned reads displayed.
TAAGGCGGGGCAAAAAATAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGTTCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACTGGAACCCAGTGATAACG > DH1/1016029‑1016326 | tAAGGCGGGGCAAAAAATAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGt > 3:1527105/1‑151 (MQ=255) aGGCGGGGCAAAAAATAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATc > 8:800074/1‑151 (MQ=255) aGGCGGGGCAAAAAATAGCGACCATGGGTACCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGCGGAAATCCGTAAACCCGCTCCGTTATTTGACGCAGCGATAAATCGGCGGAACCAGGCTTCTGCTTGAATGATc > 2:283367/1‑151 (MQ=255) gggCAAAAAATAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTc > 6:183295/1‑150 (MQ=255) gggCAAAAAATAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAACGATCCGTCa < 8:1467112/151‑1 (MQ=255) ggCAAAAAATAGCGACCATGGGTAGCACCGGAACCAGTTCAACAGGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCaa < 6:81891/151‑1 (MQ=255) cAAAAAATAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAgg > 6:1624635/1‑151 (MQ=255) aaaaaaTAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATACGTAAACCCGCTGCGGTATTTGCCGCAGAGATAAATCGGCGGAACCAGGCTTTTGCTGGAATGATCCGTCAAggg > 5:181769/1‑151 (MQ=255) aaaaaTAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGTTCCGTCAAggg > 3:328680/1‑150 (MQ=255) aaTAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATc > 2:1133647/1‑150 (MQ=255) aaTAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCa < 5:146621/151‑1 (MQ=255) aTAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCAc < 4:1274086/151‑1 (MQ=255) cGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGt < 1:1057346/151‑1 (MQ=255) cGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGt > 7:2059628/1‑151 (MQ=255) cATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGTTCCGTCAAGGGATCACGGGTAggc > 1:1933225/1‑150 (MQ=255) gggTAGGACCGGAAGCAGTTCTAGACGCTTGCATTTTGAAATTGGTTACAAGTGGAATTCCGTAAACGGTCTTCGTTTTTTGCCGCAGCGATAAATGGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGc < 6:1314242/150‑1 (MQ=255) tAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCAcc > 7:122847/1‑151 (MQ=255) aCCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTAt < 2:1028683/151‑1 (MQ=255) cGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATg > 8:445727/1‑150 (MQ=255) cGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGa < 5:591095/151‑1 (MQ=255) ggAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATg > 1:502904/1‑149 (MQ=255) ggAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATg > 4:1549492/1‑149 (MQ=255) aaCCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTc > 6:1209303/1‑151 (MQ=255) aaCCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTc > 4:1088911/1‑151 (MQ=255) aCCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTc > 1:1751182/1‑150 (MQ=255) cAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGa < 3:1953486/151‑1 (MQ=255) gTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAAt < 2:1604716/151‑1 (MQ=255) gTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAAt < 6:1764082/151‑1 (MQ=255) tCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATAc > 3:964330/1‑151 (MQ=255) tCAACACGCTTGAATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATAc > 4:926244/1‑151 (MQ=255) acacGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCt < 1:249204/151‑1 (MQ=255) acGCTTGGATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGa < 8:1859936/151‑1 (MQ=255) cGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGa > 3:133614/1‑150 (MQ=255) tGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGt < 4:196231/150‑1 (MQ=255) aTTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCAt < 1:323984/151‑1 (MQ=255) ttttGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATg > 2:875836/1‑151 (MQ=255) tttGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTTCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATg > 5:484415/1‑150 (MQ=255) ttGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGAt > 7:3998/1‑151 (MQ=255) tGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGAtt > 4:624/1‑151 (MQ=255) tGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGAt < 8:1152715/150‑1 (MQ=255) aaaTTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGAttt > 3:1029611/1‑150 (MQ=255) aaTTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTa > 3:164310/1‑150 (MQ=255) tCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGTTCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATg > 5:706215/1‑151 (MQ=255) tCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAAt < 1:7280/150‑1 (MQ=255) cGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATg < 1:1302944/150‑1 (MQ=255) gTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGa > 3:1173726/1‑150 (MQ=255) tACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGAAGAGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCTTGAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTGCATGATTTAAATGa > 2:605612/1‑148 (MQ=255) cAAGGTGAAATCCGTAAACCGGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCTTCAAGGGATCACGGGTAGGAGGCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATg < 5:1635202/151‑1 (MQ=255) cAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATg > 5:1299493/1‑151 (MQ=255) aaGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGc > 2:1731191/1‑151 (MQ=255) aaGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGc > 6:1504466/1‑151 (MQ=255) gggAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGa < 6:889052/151‑1 (MQ=255) ggAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGaa < 5:902307/151‑1 (MQ=255) ggAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGaa < 3:780770/151‑1 (MQ=255) aaaTCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAAtt < 7:740538/151‑1 (MQ=255) aaTCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAAttt < 7:957823/151‑1 (MQ=255) aTCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCTGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTtg < 5:271904/151‑1 (MQ=255) aTCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTtg > 7:624598/1‑151 (MQ=255) gTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTtgatg > 1:1605091/1‑150 (MQ=255) aaaCCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTtgatg > 3:589941/1‑148 (MQ=255) aaaCCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATgag < 7:447569/150‑1 (MQ=255) cccGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCCCGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATCATTTAAATGAAGCTGCGGAATTTGATGAGAACg > 4:318114/1‑151 (MQ=255) cGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGATCCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACgg > 5:1913279/1‑150 (MQ=255) cGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGTTCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACgg > 8:196695/1‑150 (MQ=255) cGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACgg > 7:18089/1‑150 (MQ=255) cGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGACACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACgg > 7:1349026/1‑150 (MQ=255) gCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACgg > 4:1347312/1‑149 (MQ=255) gCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAg < 2:514361/151‑1 (MQ=255) cTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGt > 2:1296291/1‑151 (MQ=255) tGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGtt > 8:1703810/1‑151 (MQ=255) tGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGtt > 5:1783518/1‑151 (MQ=255) gCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGtt < 8:322488/150‑1 (MQ=255) gCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGtt < 6:2044561/150‑1 (MQ=255) gCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGtt < 8:623722/150‑1 (MQ=255) gCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGtt < 5:780402/150‑1 (MQ=255) gCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGACAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATAAGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTg > 6:476725/1‑151 (MQ=255) ttATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAgg > 2:203467/1‑151 (MQ=255) aTTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGtt > 4:185356/1‑151 (MQ=255) tttGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGttt > 6:1132374/1‑151 (MQ=255) gCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGa > 4:1135366/1‑149 (MQ=255) aGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGaa < 6:1022182/151‑1 (MQ=255) cGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGaaaa < 3:458928/151‑1 (MQ=255) cGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGaaa > 7:1508603/1‑150 (MQ=255) gATAAATCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGaaa > 7:844377/1‑149 (MQ=255) aaaTCGGCGGAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGcc < 2:1477707/151‑1 (MQ=255) aTCGGCGGAACCAGGCTTTTGCTTGAATGTTCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCtt < 7:162445/151‑1 (MQ=255) gcggAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTtagt > 8:1353793/1‑150 (MQ=255) cggAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTtagtag < 7:1706375/151‑1 (MQ=255) ggAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGa < 5:1055348/151‑1 (MQ=255) ggAACCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCAAtagt > 4:742124/1‑148 (MQ=255) gAACCAGGCTTTTGCTTGAATGTTCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGaa < 6:394493/151‑1 (MQ=255) aCCAGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACt > 3:1741298/1‑151 (MQ=255) cggCGTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACTGGa < 5:406688/150‑1 (MQ=255) aGGCTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACTGGa < 3:1851452/151‑1 (MQ=255) cTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACTGGAAcc > 6:659989/1‑151 (MQ=255) cTTTTGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATCCGGAATTTGATGAGAACGGAGTTGATCTATTTGACGAAAAGGCCTTAGTAGAACAGGaaac > 8:846585/1‑149 (MQ=255) tttGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACTGGAAccc > 4:1759378/1‑150 (MQ=255) ttGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACTGGAACCCa < 6:514529/150‑1 (MQ=255) ttGCTTGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACTGGAACCCa < 1:1193202/150‑1 (MQ=255) ttGAATGATCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACTGGAACCCAGTGAt > 7:1982212/1‑151 (MQ=255) | TAAGGCGGGGCAAAAAATAGCGACCATGGGTAGCACCGGAACCAGTTCAACACGCTTGCATTTTGAAATTCGTTACAAGGGGAAATCCGTAAACCCGCTGCGTTATTTGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGAATGTTCCGTCAAGGGATCACGGGTAGGAGCCACCTTATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGAAAAGGCCTTAGTAGAACTGGAACCCAGTGATAACG > DH1/1016029‑1016326 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |