Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA25.4% 327.8 / 24.1 211intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (119/16);  new base A (45/1);  total (194/17)
Fisher's exact test for biased strand distribution p-value = 7.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

TACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCA  >  DH1/613625‑613920
                                                                                                                                                  |                                                                                                                                                      
ccccaaaaacaccccGGCTTAGCGGGCAGTTCCCCCGGGTTACCGTAACACCCCGCTGCGGCGGGTTTTTGGTTTTGCGGGGGCCCAAAAATGAATGCCTGGCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTg                                                                                                                                                   <  7:689517/137‑1 (MQ=2)
              cGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGATAGATGAGTGACTTTCGCCGACACTTTAGCCAAAAGAAAGC‑GGCTTATAGGTCAGTTTCACCGGTTTTAAGTAAAAAc                                                                                                                                     >  1:23748/1‑151 (MQ=11)
                  ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTTGAGGGGCAGAAATATGAATGACTGTCCCCGACACTATACACAAAAGAAAGC‑GGCTTATCGGTAAGTTAAACATTATATACGTAAAAAacccg                                                                                                                                 >  1:20875/1‑149 (MQ=11)
                    aTCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGTGCTGTTAGACCTCTTGTAAATaaaaatcagatt                                                                                                                               >  7:787217/1‑144 (MQ=11)
                      cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTTTCGACGACACTATACACAAAAGAAAGC‑GGCTTATCGGTCAGTTTTACCTGATTTACGTAAAAAACCgatccg                                                                                                                             >  6:127069/1‑146 (MQ=11)
                      cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTGTACGTAAAAACCCGCTAcg                                                                                                                             >  6:314838/1‑151 (MQ=25)
                      cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTAcg                                                                                                                             >  3:1708508/1‑151 (MQ=25)
                      cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTAAACCCAAAAGAAACC‑GGCTAATCGGTAAGATTAACATCATTTAAGTaaaaaacatgtgcg                                                                                                                             >  2:1422309/1‑142 (MQ=11)
                       ggTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTGcgg                                                                                                                            >  7:1703235/1‑151 (MQ=25)
                        gTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGGATCGGATGATTTAAGTAAATAccaccatcccc                                                                                                                           >  3:928439/1‑142 (MQ=11)
                           aGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCggg                                                                                                                        >  2:1081740/1‑151 (MQ=255)
                             tttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGGGtt                                                                                                                      >  2:630055/1‑151 (MQ=255)
                              ttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGATACGGCGGGttt                                                                                                                     >  8:1439687/1‑151 (MQ=255)
                              ttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGTTACGGCGGGttt                                                                                                                     >  6:289170/1‑151 (MQ=255)
                               tCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGGTGCGGCGGGtttt                                                                                                                    >  7:823/1‑151 (MQ=255)
                                cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGGTTCGGCGGGttta                                                                                                                    >  1:1594201/1‑149 (MQ=255)
                                 accggctatccGTAAACCCCCGCTTCGGCGGTTTTTTTTTTTTTGATTGGCAGAAATATGATTTATTCTCAACGAAACTATATCTTAAAGAAATC‑GTCTTATTTGGGATTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTa                                                                                                                  <  4:1098301/141‑1 (MQ=2)
                                 aCCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGC‑GGCGTATGGGGAACGTTCTGCTGTGGTAGGTAATTAACCGCTTCggggggggttct                                                                                                                  >  3:801017/1‑145 (MQ=11)
                                   cTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATGCGGCGGGttttaaat                                                                                                                >  4:776944/1‑147 (MQ=255)
                                    tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTGTACGTAAAAACCCGCTGCGGCGGGTTTGTGCtt                                                                                                               >  7:584557/1‑151 (MQ=255)
                                     ggTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTTTACttt                                                                                                              >  8:436194/1‑151 (MQ=37)
                                        ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTGCTTGTgg                                                                                                           >  1:1907786/1‑151 (MQ=9)
                                        ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTGCGGCGGGTTTTTAAttgttg                                                                                                           >  2:172280/1‑147 (MQ=255)
                                        ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTTATAAtttttg                                                                                                           >  2:441968/1‑149 (MQ=11)
                                         tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTGTACGTAATAACCCGGTGCGGCGGGtttgtgattgtggt                                                                                                          >  1:948430/1‑140 (MQ=11)
                                          aCGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTGTgg                                                                                                           >  5:1943974/1‑149 (MQ=9)
                                           cgaaactccccGCAGCGGGGGGTTTTTGGTTTTGTACGGGCGCAAACAGCATTGTCTCGCCCCGACTCTATTCGCACCACAAAGC‑GGCTTAGCGGGCATATTCACCTGTGTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAgg                                                                                                        <  7:314455/143‑1 (MQ=2)
                                              aaaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTTCTTGTGGAGGGGc                                                                                                     >  7:1012/1‑151 (MQ=16)
                                                 aaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGAAGAAAGATGAAGGACTGTCAACGAAACTATAGCCAAAAGAAAGG‑GGCTTAACGGTCAGTTTCAAATGTTTTACGTAAAAACCCGCGTCGGCGGGTTTTTGCTTTTGGAGGGGcggg                                                                                                  >  2:783209/1‑148 (MQ=11)
                                                     cGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAAGCTCTGAACAGCACACGACACAAAAAAACACGCATACAAAACGG‑CAGTTTCACCTGTTTTACGTAAAAAAAAGATTCGGCGGGTTGTTGGTTTTGGCgggggtgcgcgg                                                                                              >  1:621763/1‑143 (MQ=2)
                                                         tCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTACGGCGGGTTTTTCATTTTTGAGTGCCAGAAAGattat                                                                                          >  7:286153/1‑148 (MQ=11)
                                                              gggTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTATTCTTTTTGAGTGGGAGAAATatgtgaccc                                                                                      >  5:1263639/1‑144 (MQ=11)
                                                                 tttttGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATGCGGCGGGTTTCTACTTTTGGAGTGCCATTAAgaagaaaccctggc                                                                                  >  3:1296176/1‑143 (MQ=11)
                                                                     tgattttGGAGTGGTAGAAAGTTGAATTAATTTTTACGATACTATACGCTATAGATATC‑TGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCAc                                                                               <  8:227684/147‑1 (MQ=11)
                                                                        ttttGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTATGCTTTTGGAGTGCCAGAAAGATGAATGCCATTCCACg                                                                              >  7:356341/1‑148 (MQ=11)
                                                                        ttttGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTAGTTTTGGAGGGGCAGAATGATGAATGACTGTCCagtcc                                                                            >  7:1055302/1‑146 (MQ=255)
                                                                                    aGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATTCGGCGGGTTTGTACTTTTTGAGTGGCAGAAATAACTAACACTTTCCACGacaagaaagacaaa                                                                >  2:1672326/1‑139 (MQ=11)
                                                                                     gAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGGTTCGGCGGGTTTTTACTTTTTGAGTGGCAGAAAGAGGAAGGACATTCCACGACACtagcgcacaaac                                                              >  7:877413/1‑141 (MQ=11)
                                                                                      aaaGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGATACGGCGGGTTTTTACTTTTTGAGGGCCAGAAATATGAAAGAGTTTCCACGacacagtagaaaaa                                                                >  8:1211987/1‑138 (MQ=11)
                                                                                             aatgaCTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTCCTTTTGGAGGGCCAGAAAGAGGAATCCCTGTCCACGACACGATACCCAAAAGAATGCGGc                                                      >  7:1388207/1‑151 (MQ=11)
                                                                                               tgaCTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTGCGGCGGGTTTGTGCTTTTTGAGGGGCAGAAACATCAATGACTGGCCACGTCAATATACACAAAAGAAAGCCGcat                                                    >  1:1425260/1‑149 (MQ=11)
                                                                                               gccccgccgccgccacTACCCCCAAACCAACGC‑GGCTAAGCGCGCAGTTGCCCCTGTTTTACGTAAAAACCCGCTGCGGCGGTTTTTTTCTTTTGGTGGGGAAGAACCATGAATGACTGACCACGACACTATACCCAAAAGAAAGCGGCtt                                                    <  3:1894739/138‑1 (MQ=2)
                                                                                                gaCTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTTTACTTTTGGAGTGGCATAAATATGAAACACTTTCCACGACAATATACACAaaagaaaccaactt                                                    >  8:1090335/1‑143 (MQ=11)
                                                                                                 aCTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGGTGCGGCGGGTTTGTAATTTTGGAGGGGCAGAAAGATGAATCGCTGTCCCCGACACTAAAAGAAAAAGAAAGCTCCTTa                                                   >  3:478865/1‑150 (MQ=11)
                                                                                                    taactctttacTATACCCAATCTATTGC‑GCTTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCg                                                <  6:279195/141‑1 (MQ=11)
                                                                                                     tCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTTTTACTTTTGGAGGGGGAGAAAGAGTAAAGACTGTCGACGACACTATACGCAAATGAAAGCGGCTTATCgg                                               >  6:1724386/1‑150 (MQ=11)
                                                                                                         cGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGATTTACGTAATAAACCGATACGGCGGGTTTGTACTTGTTGAAGGGCAGAAGGAAGAATGCGTGTCCACGACAGTATACCCAAAAGAAAGAGGCTTATCGGTCAGt                                          >  6:915514/1‑151 (MQ=11)
                                                                                                            cacTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTTGTACTTTTGGAGGGGCAGAAAGAGGAAACACTTTCCACGACACTATACGCAAAAGCAAGCAGCATATCGCACAGtatt                                       >  2:331709/1‑151 (MQ=11)
                                                                                                            cacTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGGAGAAAGATGAATCACTGTCGACGACACTATCCCCAACAGCAAGCGGCTGATCGGTCAGtttt                                       >  3:1123747/1‑151 (MQ=11)
                                                                                                             acTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGACACTATAAACAAAAGAAAGCGGCTTATCGGTCAGtttt                                       >  3:862567/1‑150 (MQ=255)
                                                                                                              cTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACAGTCGACGACACTATAAAAAAAAGAAAGCGCCTTAACGGTCAGTATTa                                      >  6:966531/1‑150 (MQ=11)
                                                                                                               tataCCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAATATGAATGCCTTTCCACGACACTATCCGCAAAAGAAAGCGGCTTATCGGTAAGTTTTAcc                                    >  1:1366838/1‑151 (MQ=11)
                                                                                                                ataCCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTACTTTTTGAGGGGGAGATAGATCAAACACTGTCCACGACACTATACGCAAAAGAAAGCCGCTTATCGGACAGttaaaact                                   >  3:1385814/1‑145 (MQ=11)
                                                                                                                ataCCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCt                                   >  5:753761/1‑151 (MQ=35)
                                                                                                                 taCCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTTTACTTTTTGAGGGGCAGAAAGATGAAAGACTGGCCACGTCACTATCGACAAAAGAAAGCTGCAGATCGGTCAGTGTTAACTg                                  >  4:1070610/1‑151 (MQ=11)
                                                                                                                 taCCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGAGGAATGACTGTCCACGACACGATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTTAcatt                                  >  3:1586647/1‑148 (MQ=255)
                                                                                                                 taCCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAAAGCCTGTCCGCGACACTAGCCACAAAAGAAAGCGCCTGAACGGACAGTGTTACCTg                                  >  4:189995/1‑151 (MQ=11)
                                                                                                                  aCCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCATATAGATGAATCACTGTCGACGACACTATAGCCAAAAGAAAGCGGCTTATCGGTCAGTATTACCTgt                                 >  4:1559643/1‑150 (MQ=11)
                                                                                                                  aCCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTg                                  >  5:47704/1‑150 (MQ=35)
                                                                                                                    ccAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGagg                               >  1:1100896/1‑149 (MQ=37)
                                                                                                                    ccAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGAAGAATGACTGTCCACGACACTATAAACTAAAGAAAGCGGCTTATCGCTCAGtgtaaaaagatg                               >  2:15115/1‑140 (MQ=11)
                                                                                                                     cAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCCCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGt                              >  5:677609/1‑151 (MQ=39)
                                                                                                                     cAAAAGAAAGC‑GGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGATAGATGAATGACTGTCAACGACACTATACACAAAAGAACGCGGATTATCTGTCAGTTTTACCTGATGt                              >  7:967526/1‑151 (MQ=14)
                                                                                                                      aaaaGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTATTACATGATGt                              >  6:1025946/1‑150 (MQ=255)
                                                                                                                         agaaagC‑GGCTTATCGGTCTGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTGTACTTTTGGAGGGGCAGAAATATGAATGCCTGGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGt                          >  5:1639329/1‑151 (MQ=255)
                                                                                                                           aaagC‑GGCTTATCGGTCAGTTTCATCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGta                         >  7:507471/1‑150 (MQ=255)
                                                                                                                           aaagC‑GGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTTTTACTTTTGGAGGGCCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCATTTTTAAATGATGTACGta                         >  6:1008495/1‑150 (MQ=14)
                                                                                                                             agC‑GGCTTATCGGTCAGTTTGACCTGGTTTACGTAAAAACCCGGTTCGGCGGGATTTTAATATTGGACGTGGGGAATGAAGAATGACTCTCAAAGAGACTATGCCCAAAAGACAGAGGAATATAGGTCAGTTTGCCCTGTTGTACGtaat                       >  1:1150310/1‑150 (MQ=2)
                                                                                                                               c‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTTGAGGGGCAGATATATGAATCACAGTCCACGACACTATACAAAAAAGAAAGCCGCTTATCGCACAGTTTTACATGATGTACGtaataa                     >  2:724998/1‑150 (MQ=14)
                                                                                                                               c‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaataa                     >  1:970773/1‑150 (MQ=255)
                                                                                                                               c‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaataa                     >  6:1303110/1‑150 (MQ=255)
                                                                                                                               c‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAAGGACTGTCCACGACACTAACCCCAAAAGAAAGCGGCTGATCGGTCAGTTTTACCTGAGGTACGtaataa                     >  1:1459063/1‑150 (MQ=14)
                                                                                                                                ttttttttttttCATTTTCACATGTTTTATTTAAAAACCGGCTTCGTCTGTTTTTTACTTTTGGAGGGTCTTAATTTTTAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaataa                     <  8:532743/139‑1 (MQ=14)
                                                                                                                                 ggttccgatgTCAGTTTCACCTTTTTTACGTAAAAACCCGCTTCGGTTTTTTTTTTTTTTTGGTGGGGCAGAAATATTAATGACTGTCCACGAAACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                   <  8:1468078/142‑1 (MQ=14)
                                                                                                                                 ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAACTGATCTACGTAATaaa                    >  1:1311983/1‑150 (MQ=255)
                                                                                                                                 ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGATTCGGCGGGTTTATGCTTTTGAAATCGCAAAAACATCAATGACTGTCAGAGACACTAAACCCAAAAGAATGAGGCTTATCGCTCACTTTTAAATGATATACGtaataacc                   >  4:1598628/1‑149 (MQ=2)
                                                                                                                                  gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                  >  5:708357/1‑151 (MQ=255)
                                                                                                                                   cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGAGACTATACCCAAAAGAAAGCCGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                 >  5:885336/1‑151 (MQ=255)
                                                                                                                                   cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                 >  7:1913896/1‑151 (MQ=255)
                                                                                                                                   cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATCACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAACTGATGTACGTAATAAACCg                 >  5:375191/1‑151 (MQ=255)
                                                                                                                                       tCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACAGTTcc             >  5:1069285/1‑151 (MQ=255)
                                                                                                                                        cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg            >  2:1161686/1‑151 (MQ=255)
                                                                                                                                        cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg            >  2:505811/1‑151 (MQ=255)
                                                                                                                                        cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg            >  3:563969/1‑151 (MQ=255)
                                                                                                                                        cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAACCCGGTCcg            >  3:1482136/1‑151 (MQ=255)
                                                                                                                                          gtacgTTTCTCCTGTTTTACGTAAAACCCGGTTTCGGGGGTTTTTTTATTTTGGCGGGGCACAAATTTGATTGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg           <  2:1918179/146‑1 (MQ=17)
                                                                                                                                          gTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCGACGACACTATACCCATAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTAGGTAATTAACCGTTCcggc          >  6:1877604/1‑151 (MQ=255)
                                                                                                                                          gTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc          >  5:424711/1‑151 (MQ=255)
                                                                                                                                           tCAGTTTCACCTGATTTACGTAAAAACAAGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAAACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGtgataggaagagggtggg         >  4:1179103/1‑138 (MQ=11)
                                                                                                                                            cAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg        >  5:1881559/1‑151 (MQ=255)
                                                                                                                                             aGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg       >  5:1314143/1‑151 (MQ=255)
                                                                                                                                              gTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGt      >  4:137498/1‑151 (MQ=255)
                                                                                                                                              gTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGt      >  2:898919/1‑151 (MQ=255)
                                                                                                                                               tttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTAGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCCGCTTATCGCTCAGTTTAACCTGATTTACGTAATAAAACGATACGCCGGCtt     >  8:1854248/1‑151 (MQ=11)
                                                                                                                                                ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt    >  4:27149/1‑151 (MQ=255)
                                                                                                                                                ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGgtt    >  1:719783/1‑151 (MQ=255)
                                                                                                                                                 tCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc   >  3:107112/1‑151 (MQ=255)
                                                                                                                                                  cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACGTGATGTACGTAATAAACCGTTACGGCGGGTTTc   >  8:1602134/1‑150 (MQ=255)
                                                                                                                                                  cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc   >  8:613286/1‑150 (MQ=255)
                                                                                                                                                  cACCTGTTTTACGTAAAAACCCGCTTCGGCGCGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTCTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGAATTACCTGATGTACGTAATAAACCGTTCCGGCGGGTATc   >  6:560014/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
TACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGC‑GGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCA  >  DH1/613625‑613920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑