Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.40.9% 227.1 / 176.8 112intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (7/58);  new base . (2/43);  total (9/103)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.27e-01

Only 100 of 131 total aligned reads displayed.

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGG  >  DH1/936019‑936311
                                                                                                                                                      |                                                                                                                                               
gAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                                 >  8:1607811/1‑151 (MQ=255)
 aaTGGCGCTTTTTTATGGTAGTCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                                <  6:1522770/151‑1 (MQ=255)
 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                                <  3:1866907/151‑1 (MQ=255)
 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                                >  7:527064/1‑151 (MQ=255)
  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                               <  6:1593711/151‑1 (MQ=255)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAAGACCCTTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttac                                                                                                                                             <  1:857039/151‑3 (MQ=255)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGGCGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                             <  7:104390/151‑1 (MQ=255)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                              <  7:43821/150‑1 (MQ=255)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                              <  1:1014682/150‑2 (MQ=255)
     gcgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttact                                                                                                                                            >  5:1207463/1‑148 (MQ=255)
     gcgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAc                                                                                                                                            <  5:913742/151‑1 (MQ=255)
     gcgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAc                                                                                                                                            <  6:158715/151‑1 (MQ=255)
      ggcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                                           <  4:728627/150‑1 (MQ=255)
      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                                           <  3:696014/151‑1 (MQ=255)
      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                                           <  3:1640200/151‑1 (MQ=255)
       tcTTTTTTATGGGAGACCAGAAACAATAAAAGACGGTTTATGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                           <  6:581504/149‑5 (MQ=17)
       gcTTTTTTATGGGAGACCAGAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtc                                                                                                                                         <  5:1261687/151‑1 (MQ=255)
       gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACtt                                                                                                                                          <  4:367070/151‑1 (MQ=255)
       gcTTTTTTATGGGAGACCAGAAAAAAAAAAACACGCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtc                                                                                                                                         <  2:880805/151‑1 (MQ=255)
       gcTTTTTTACGCGACACCTGATACAAATAAACCGGTTTTTGTGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtc                                                                                                                                         <  1:391212/151‑1 (MQ=11)
        gtattttttTGGAGACCAGAAACAAATAATCATCATTTTGGGTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                        <  6:1205931/148‑1 (MQ=14)
        cgtttttATGGGAGACCAGATACAAAAAAAGCCGCGTTAGGGTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                        <  5:933847/149‑1 (MQ=17)
        cgtgtttAGGGGAGACCAGTTACAACAAATGACCCTTTATGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                        <  1:1025187/147‑1 (MQ=14)
        cTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttacgtc                                                                                                                                          >  7:1903961/1‑145 (MQ=255)
         gtttttATGGGAGACCAGAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGCGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcat                                                                                                                                       <  3:1789279/150‑1 (MQ=255)
          tttttATGGGAGACCCGAAACAAAAACACAGGATTTAGGGTGTTCTATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcat                                                                                                                                       <  4:461126/150‑1 (MQ=14)
          tttttATGGGAGACCAGAAACAAAAAAACACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                        <  1:10121/149‑1 (MQ=255)
          tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtca                                                                                                                                        <  1:1165675/150‑1 (MQ=255)
          tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatc                                                                                                                                      >  4:1774083/1‑151 (MQ=255)
            ttttTGGGAGACCAGAAACAAAAAAAGAGCCTTTATGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatca                                                                                                                                     <  4:779282/150‑1 (MQ=17)
            tttATGGGAGACCAGAAACAAAAAATGACCCTTGTGGCTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatca                                                                                                                                     <  7:212408/150‑1 (MQ=14)
             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                     <  1:813142/150‑1 (MQ=255)
             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                     <  3:730913/150‑1 (MQ=255)
             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                     <  4:1751412/150‑1 (MQ=255)
             ttATGGGAGACCACTAAAAAATAAACACCTTTTAGGGTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaa                                                                                                                                    <  2:1120625/150‑1 (MQ=14)
             ttAGGGGAGACCTGTAAAAACTACAGATCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaa                                                                                                                                    <  6:1276560/150‑1 (MQ=11)
              tATGGGAGACCCGATCAAAAAAAACCGCCGTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaaa                                                                                                                                   <  3:822795/150‑1 (MQ=14)
              tATGGGAGACCAGAAACAAAAACACACCCTTTAGGGTGTTCATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaaa                                                                                                                                   <  2:1554973/150‑1 (MQ=17)
              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                    <  1:45161/150‑1 (MQ=255)
              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                                    <  3:1714252/150‑1 (MQ=255)
               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                                   <  4:826090/150‑1 (MQ=255)
               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                                   <  3:269841/150‑1 (MQ=255)
               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                                 <  2:1709803/151‑1 (MQ=255)
               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                                 <  6:494524/151‑1 (MQ=255)
               aTGGCAGACCAGCAACAACAAAACACCCGTGAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                                <  3:1778107/151‑1 (MQ=17)
                tGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                                 <  4:1088560/150‑1 (MQ=255)
                tGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaa                                                                                                                                    <  5:1952152/147‑1 (MQ=255)
                ggggacaccccataCAAAAACACCCCCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                                <  3:1884055/137‑1 (MQ=14)
                 gggAGACCAGAAACAAAAAACCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                               <  8:1847294/151‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACGCTTTTGGGTGTACAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttt                                                                                                                               <  6:1136507/150‑1 (MQ=17)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                               <  4:1606077/151‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑tt                                                                                                                                <  2:1857063/150‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑tt                                                                                                                                <  7:163654/150‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                              <  8:574969/151‑2 (MQ=255)
                  ggAGACGAGAAACAATAAAACACCGTTTATGGTGTTCAATAATTGGTTGCGGGGTCGGGATTTTAACCGAATACGTTCGGGTTATGAGCCCGACGATCTACCAGGCTGCTCCACCCCTAGTAACCGTACTGCTTTTACTTCATCAAA‑TTTg                                                                                                                              <  6:395272/151‑1 (MQ=17)
                  ggAGACCAGATACAAAAAAAGACGTTTTTTGTTGTTATATAATTGGTTGCGGGTGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                              <  8:1617245/150‑2 (MQ=11)
                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTg                                                                                                                              <  7:158151/151‑1 (MQ=255)
                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTg                                                                                                                              <  3:1249655/151‑1 (MQ=255)
                  ggAGACCAGAAAAAACTAAACCCCTGTTAGGTTGTTAATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttta                                                                                                                             <  2:182192/151‑3 (MQ=11)
                   gagaCCAGAATAAAATATAGACCCTTTAGGGTGTTAAATAATTGGTTGCGGGGGGCGCTTTTGAACCGACGACCTTCTGGTTATGAGCCCGACGAGGTACCAGGCTGCTCCACCCCGCGTCACGGTACTGCTTTTACTTCATCAAA‑TTTGa                                                                                                                             <  2:1173016/151‑1 (MQ=17)
                   gagaCCAGAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaa                                                                                                                            <  7:1108386/151‑4 (MQ=255)
                    agaCCAGAAACAAAAAAACACCCGTTAGTGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAt                                                                                                                            <  1:1808559/151‑1 (MQ=255)
                    agaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGGTGCGGGGGCCGGATTTGACCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAt                                                                                                                            <  8:1477964/151‑1 (MQ=255)
                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAtt                                                                                                                           <  7:1268949/151‑1 (MQ=255)
                     gaCCAGAAAAAAATAAACACCTTTTAGGGTGTTAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                          <  8:1901872/151‑6 (MQ=11)
                     caCCCGAAACCAAAAAACCCGCTTCATCGTGATCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                          <  1:1507754/150‑6 (MQ=1)
                      aCCAGATACAAAAAAAGCCGCTTTAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattg                                                                                                                         <  3:741448/151‑7 (MQ=11)
                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTgg                                                                                                                         <  5:433260/151‑1 (MQ=255)
                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTgg                                                                                                                         <  4:1624635/151‑1 (MQ=255)
                        cacaaaCAAAAACACACCCGTTAGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattggt                                                                                                                       <  1:1484625/148‑9 (MQ=14)
                        cAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGt                                                                                                                        <  3:243814/151‑1 (MQ=255)
                         cgAAACAACAAAAGACCGGTTAGGTTGATCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGtt                                                                                                                       <  5:644248/150‑1 (MQ=11)
                         ccaaaCAAAAACACCGCCGTTAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGtt                                                                                                                       <  1:547167/149‑1 (MQ=11)
                         agtaaaaaaaaaaCACCTGTTATGTTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGtt                                                                                                                       <  8:526636/145‑1 (MQ=11)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTTCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                       <  2:277657/151‑1 (MQ=255)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                       <  8:173463/151‑1 (MQ=255)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                       <  5:513835/151‑1 (MQ=255)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACGTCATCAAATTTTAATTGGtt                                                                                                                       >  2:546719/1‑151 (MQ=14)
                         aGAAAAAAAAACACACCCGTAAGGGTGTAAAATAATTGGTTCCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGtt                                                                                                                       <  5:1150069/151‑1 (MQ=11)
                          gtacccaatacacacCTGTCTGGGTGTTAATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTg                                                                                                                      <  2:843092/140‑1 (MQ=11)
                          cgtgaaaaaacaCCCCCGTCAGCGTCATAATTAATTGGTTGCGGGGGCCGGATGTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTg                                                                                                                      <  7:1655674/146‑1 (MQ=11)
                           accacataaaaCCCCCGTCAGCTTCTTTAAATATTGGTTGCGGTGGCCGGATTTGACCCGACCCCCTTCGGGTTATCAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGc                                                                                                                     <  6:822963/144‑1 (MQ=14)
                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGc                                                                                                                     <  5:1073606/151‑1 (MQ=255)
                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTCGTTGCGGGTGCCGGATTTGACCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGc                                                                                                                     <  4:1208963/151‑1 (MQ=255)
                           aaaCAAAAAAACACCCGGTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGc                                                                                                                     <  7:926700/151‑1 (MQ=255)
                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCg                                                                                                                    <  6:567088/151‑1 (MQ=255)
                              cAAAAATACTCCCGTTTGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAAGCGAGGTGCTTCGGGTTATGAGCCCGACGAGCTAGCAGGCTGCTGCACCCCGCGTGACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTgggg                                                                                                                   <  4:1573460/150‑1 (MQ=17)
                               aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCgggg                                                                                                                 <  6:619137/151‑1 (MQ=255)
                               aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCgggg                                                                                                                 <  5:1377880/151‑1 (MQ=255)
                                aaaataCTCCCGTTAGGGTGTTCAATATTTGGTTGCGGGGGCCGGATTTGAACCGACGTGCTTCGGGTTATGAGCGCGACGAGCTAGCAGGCTGATCCACCCCGCGTCACCGTACTGATTTTACTTCATCAAA‑TTTGATTGGTTGCggggg                                                                                                                <  8:1752475/151‑1 (MQ=17)
                                aaaaaaGACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                               <  7:1835698/121‑1 (MQ=255)
                                 aaaaaCTCCAGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGGGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGc                                                                                                               <  6:723995/151‑1 (MQ=255)
                                 aaaaaCACCTTTTAGGTTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattgg                                                                                                                        <  6:508069/141‑8 (MQ=11)
                                 aaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACGTTCTCAAAA‑TTTTATTggagcgcgga                                                                                                                 >  5:508709/1‑141 (MQ=14)
                                  aaaaCACCCTTTTGGGTGTTCAATTATTGGTTGCGTTGGCGGGATTTGTACCCTCGACCTTCGGGTTATCAGCCCGACTAGCTTCCAGTCTTAGCCACCCCTCTTAACATTAATGCTTTTAATTGATCATA‑TTTGATTGGTTGCGGGGGcc                                                                                                              <  4:1018341/151‑1 (MQ=255)
                                  aaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGcc                                                                                                              <  7:894751/151‑1 (MQ=255)
                                          ggTTAGGGTGTTCAGTAATTGTTTGCGGGGGGCCGAGGTGCCCCCCCGCGCTTCGGGTAAACGGCCCGCCCAGCCACCAGGCTCCTGGAGACCCCGTCACCGTACTCCCGTTTCATGCTCTTA‑ATTCATTGGTTGCGGGGGCCGGATTTGa                                                                                                      <  5:1804596/150‑1 (MQ=255)
                                                                                     cgacgaCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAATTAAACAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACcgacga                                                                                              >  7:1514855/1‑116 (MQ=255)
                                                                                                                                           cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCg                                                                                        >  7:941461/1‑68 (MQ=255)
                                                                                                                                            aCCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTAGTCCTATACTTCAGCAAAGTATAATTGCTTGAggg    >  1:329831/1‑151 (MQ=17)
                                                                                                                                                      |                                                                                                                                               
GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGG  >  DH1/936019‑936311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑