Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.38.3% 260.8 / 192.5 131intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (64/15);  new base . (48/1);  total (114/17)
Fisher's exact test for biased strand distribution p-value = 4.79e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-04

Only 100 of 135 total aligned reads displayed.

TCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGATT  >  DH1/936146‑936443
                                                                                                                                                    |                                                                                                                                                       
ggcACCCCGCTTCCCCGTACTGCGGGTACTGCGTGATATTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTGACCGTACTGC‑TTTACTTCATCAAAtttt                                                                                                                                                      <  3:1917883/149‑1 (MQ=14)
             aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTAGTCC‑TATACTTCAGCAAAGTATAATTGCTTGAggg                                                                                                                                         >  1:329831/1‑151 (MQ=17)
                   cTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCAACCCAACCTCAACGTAGTGC‑TATACATAATCATATTATAATTAGTTCCggggggagc                                                                                                                                   >  8:110079/1‑147 (MQ=17)
                                                                     cacgacCTTCGGGTTATGAGCCCTAGGAGCTTGCAGTCCTGACCTCGGCTCTTCAGCTTACTGC‑TTTAATTCATCAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                                                 <  7:110237/150‑1 (MQ=11)
                                                                           cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGTAGTCG‑GTTACTTCATCAAATTTTAATTGATTGCGGGTGGGTGATTTGAACCTCAGACCTTAGGGTTATGATACAGACGAGCTAACACGCTGCTCCAc                                                                            >  2:1300518/1‑150 (MQ=11)
                                                                                      gAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTg                                                                >  7:436046/1‑151 (MQ=255)
                                                                                             acgaGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATgcgc                                                         >  3:640703/1‑151 (MQ=255)
                                                                                             acgaGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATgcgc                                                         >  3:635865/1‑151 (MQ=255)
                                                                                               gaGCTACCAGGCTGCTCCACCCCGCGTCCCCGTAGTGC‑GTTAATTCATCAAAGTTTATTTGGTTCCGGGGTCAGGATTTGACCCGCCCCCCTTCGGGTTTAGAGGCCGACTAGCTACAACGCTACTCCCGACCGCGTCCGTCGATgatccg                                                       >  7:720225/1‑146 (MQ=9)
                                                                                                       aGGCTGCTCCACCCCGCGTCCCGGTAGGGC‑GTTTCTTCATCAAATTTTAATTGGTTCCGCGGGCCGGATTTGCACCGAACCCCTTCGGGTTATGAGACCGACGAGCGACCAGGCTGCTGCCCACAGAGTCCGTGGATgatccgtaaagtcc                                               >  2:167278/1‑138 (MQ=9)
                                                                                                        ggCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGCCCCGCCGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCg                                              >  1:1213655/1‑151 (MQ=255)
                                                                                                                cACCCCGCGTCACCGTACTGCTTTTAATTAAGCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGGAGTATACTCCGCTGGGGtt                                      >  2:641264/1‑151 (MQ=11)
                                                                                                                cACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                      >  5:327120/1‑151 (MQ=255)
                                                                                                                 aCCCCGCGTCACCGTACTGCTTTTACTTAATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGACCGACGAGCTACCAGGCTGCTCCAACCCGCGTCCCTGGATGGGCACTATACTACTCACGAGttt                                     >  3:1097339/1‑151 (MQ=11)
                                                                                                                 aCCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTGGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                     >  7:655306/1‑151 (MQ=255)
                                                                                                                  cctcgcgTCACCGTACTGC‑TTTAACTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                                    <  3:64745/148‑1 (MQ=255)
                                                                                                                  ccccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                                    >  3:1623406/1‑151 (MQ=255)
                                                                                                                  ccccGCGTCACCGTACTGC‑TTTACCTCCACAAATTTTCATTGGTTGCGGCGGCCGGATTTGAACCGACGACCTTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                                    <  1:641889/151‑1 (MQ=18)
                                                                                                                   cccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAGTATACTACGCTCGCGTTTTc                                   >  3:1207895/1‑151 (MQ=11)
                                                                                                                     cgcgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTACGCTAGAGttttagt                                 >  4:64711/1‑148 (MQ=11)
                                                                                                                     cgcgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACTCCGCTCGATATTTCAt                                 >  1:107853/1‑151 (MQ=11)
                                                                                                                      gcgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGGGGAGTATACTGCGCTGGAGTTTTGATg                                >  7:1460414/1‑151 (MQ=11)
                                                                                                                      gcgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                                >  7:1110541/1‑151 (MQ=255)
                                                                                                                       cgTCACCGTACTGG‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGGTTTCATGc                               >  4:1332665/1‑151 (MQ=255)
                                                                                                                       cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                               >  7:1165375/1‑151 (MQ=255)
                                                                                                                        gTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAGTATACTCCGCTAGGGTTTTCATGc                               >  1:1868508/1‑150 (MQ=11)
                                                                                                                        gTCACCGTACTGC‑TTTTAGTCATGAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                              <  7:397279/151‑1 (MQ=255)
                                                                                                                         tCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGGGGAGAATACTCCGCAAGGGTTTTCGTGccc                             >  4:1739396/1‑149 (MQ=11)
                                                                                                                         tCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAATCGCAAAATACTCCGCTAGAGTTTTCATGCac                             >  2:285611/1‑150 (MQ=1)
                                                                                                                         tCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAGTCGCAAAATACTCCGAAAGTGTATTCATGCac                             >  2:796769/1‑150 (MQ=11)
                                                                                                                         tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                              >  2:1694021/1‑150 (MQ=255)
                                                                                                                          cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGGGGAGTATACTCCGATCGCGTTTTCATggacc                            >  5:1442361/1‑147 (MQ=11)
                                                                                                                          cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCCGTCAATACTCCCCACGAGTATTCATGCCAc                            >  1:463702/1‑151 (MQ=11)
                                                                                                                          cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAAGCGCTATATACTACGAAAGAGTATTCATGCacc                            >  6:636404/1‑149 (MQ=11)
                                                                                                                           aCCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                           >  1:350849/1‑151 (MQ=255)
                                                                                                                           aCCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                           >  6:38268/1‑151 (MQ=255)
                                                                                                                            ccGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCCCAATATACTCCAATATATTTTTCATTCAACCt                          >  2:1061854/1‑151 (MQ=11)
                                                                                                                            ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                          >  7:376836/1‑151 (MQ=255)
                                                                                                                             cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGGACTATACTACGCTATGGTTTTCATGCAACCtt                         >  4:370923/1‑151 (MQ=11)
                                                                                                                              gTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                        >  8:1209447/1‑151 (MQ=255)
                                                                                                                               tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGCTGTATACTACGATCGCGTTTTCATTCAACCttt‑t                      >  3:1731308/1‑151 (MQ=14)
                                                                                                                               tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGACCCCCACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAAGGGCAAAATACAACGCTAGTGTTTTCATTCCAcccgat                       >  2:312474/1‑147 (MQ=11)
                                                                                                                               tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑t                      >  3:265847/1‑151 (MQ=255)
                                                                                                                               tACTGC‑TTTACTTCATCAAATTTTAATTGGGTGCGGGGGCCGGATTTGCACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCCTGCCCCCttt‑t                      >  4:145970/1‑151 (MQ=21)
                                                                                                                                attccgttctcTTCATAAAATTTT‑ATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑t                      <  6:819928/140‑1 (MQ=18)
                                                                                                                                aCTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑tt                     >  3:1615957/1‑151 (MQ=255)
                                                                                                                                 cTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑ttt                    >  1:1212648/1‑151 (MQ=255)
                                                                                                                                 cTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtgt‑ttt                    >  2:680597/1‑151 (MQ=255)
                                                                                                                                  tgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACAATACTCCCATCGGGATTTCCTGCACCCgtg‑tttt                   >  7:1773540/5‑151 (MQ=11)
                                                                                                                                  tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCttt‑tttt                   >  5:701592/1‑151 (MQ=255)
                                                                                                                                  tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑tttt                   >  3:1843957/1‑151 (MQ=255)
                                                                                                                                   gcttttACTTC‑TTAAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                                <  6:841887/135‑1 (MQ=17)
                                                                                                                                   gC‑TTTACTTGAACAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑tttt                   <  5:543289/150‑1 (MQ=255)
                                                                                                                                   gC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑ttttt                  >  4:144988/1‑151 (MQ=255)
                                                                                                                                   gC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑ttttt                  >  2:1822733/1‑151 (MQ=255)
                                                                                                                                    c‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGACCGTGGATGCGCACTATACTCTGCTCGCGTTTTCATGCAACCtgt‑ttttt                  >  8:1257099/1‑150 (MQ=255)
                                                                                                                                    c‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCTGCTACACCCCGCGTACGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑ttttt                  >  1:1304139/1‑150 (MQ=255)
                                                                                                                                     ttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCCACTATACTCCGCTATCGATTTCATGCAACCTTT‑TTTTTa                 >  6:1376989/2‑150 (MQ=17)
                                                                                                                                     ttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTATGGGCAGTATACTCCGATCGAGTTTTCCTGGACCGTTG‑TGTTTAc                >  7:1266031/2‑151 (MQ=11)
                                                                                                                                      tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAc                >  5:1175850/1‑151 (MQ=255)
                                                                                                                                      tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAc                >  6:1777669/1‑151 (MQ=255)
                                                                                                                                      tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAc                >  4:818454/1‑151 (MQ=255)
                                                                                                                                      tttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACAATACTCCACTCTATATTTCATGCAACCt                          >  8:1857343/1‑141 (MQ=14)
                                                                                                                                       ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAcc               >  1:799255/1‑151 (MQ=255)
                                                                                                                                       ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGGGGTATACTTCGCTGTGGTTTTCTTTGAGCCttt‑tgtataacc              >  4:977401/1‑143 (MQ=11)
                                                                                                                                        aaCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAccc              >  5:230856/2‑151 (MQ=255)
                                                                                                                                         tactcatacaaTTTTTATTGGTTGCGGGGGCCGGATTTCCACCGACGTCCTTCGGGATATCATCCCGACGAGGTACCAGGCTCCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAccc              <  1:1857130/141‑1 (MQ=21)
                                                                                                                                         aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAcccc             >  1:1555752/1‑151 (MQ=255)
                                                                                                                                         aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAcccc             >  4:33840/1‑151 (MQ=255)
                                                                                                                                         aCTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCACGACGAGCTACCAGGCTGCTCCACCCAGCGTCAGTCGATGCGCAATATACAACACTCGCGATTTCATGCAACCTTTATTTTTAccac             >  1:706982/1‑149 (MQ=14)
                                                                                                                                          cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGAGGCCCTTCGGGTTATCAGCCCGACGCCCTCCCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCTTT‑TTTTTACCCCg            >  4:1081308/1‑151 (MQ=25)
                                                                                                                                          cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCg            >  8:19856/1‑151 (MQ=255)
                                                                                                                                          cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCTATATACTACGCTAGCTTATTCCTTCAGCCtgt‑tagatgggaacc           >  5:820900/1‑140 (MQ=11)
                                                                                                                                          cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAAGCGCACTATACTCCGCTAGAGTATTCATGCAACCTTA‑TTTTTAacccc            >  2:1712204/1‑149 (MQ=17)
                                                                                                                                          aattcataaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCg            <  2:1866801/144‑1 (MQ=255)
                                                                                                                                           ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCg            >  1:1372638/1‑150 (MQ=255)
                                                                                                                                           ttCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGAACTATACTCCGCTAGCTTTTTCATTCAACCGTT‑GTTTTACCCCCAt          >  6:1038283/1‑151 (MQ=17)
                                                                                                                                             catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACACCGATc         >  8:432095/1‑151 (MQ=255)
                                                                                                                                             acataaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc         <  3:1451035/147‑1 (MQ=255)
                                                                                                                                              atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTGGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc         >  1:792392/1‑150 (MQ=255)
                                                                                                                                              atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc         >  2:704666/1‑150 (MQ=255)
                                                                                                                                              atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc         >  1:689528/1‑150 (MQ=255)
                                                                                                                                              atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc         >  3:43052/1‑150 (MQ=255)
                                                                                                                                              atcaAATTTT‑ATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCAc       <  8:1458982/151‑1 (MQ=255)
                                                                                                                                               tcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACTGGAAGCGAACTATACTACACTAGAGTATTCATTCAACCTTA‑ATTTTAACACCATCAcc      >  6:237139/1‑150 (MQ=11)
                                                                                                                                                taaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACTACCTTCGGGTTATGAGCCCGACGAGCTACGAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc         <  4:1095923/147‑1 (MQ=255)
                                                                                                                                                caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACg      >  1:1878538/1‑151 (MQ=255)
                                                                                                                                                caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACg      >  8:774420/1‑151 (MQ=255)
                                                                                                                                                caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAACCAGATCACg      >  6:1064468/1‑151 (MQ=255)
                                                                                                                                                caAATTTTAATTGGTTGCGGGGGCCGGATTTCAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATCCAACCTAT‑TTTTTACCCCGATCACg      >  5:987703/1‑151 (MQ=255)
                                                                                                                                                 aaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg     <  4:764175/151‑1 (MQ=255)
                                                                                                                                                 aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg     >  3:1178960/1‑151 (MQ=255)
                                                                                                                                                 aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAACCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg     >  2:1933019/1‑151 (MQ=255)
                                                                                                                                                  aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg     >  6:83145/1‑150 (MQ=255)
                                                                                                                                                  aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg     >  7:653811/1‑150 (MQ=255)
                                                                                                                                                  aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑ATTTTACCCCGATCACgg     >  6:926647/1‑150 (MQ=255)
                                                                                                                                                   aTTTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGAt   <  6:1440514/151‑1 (MQ=255)
                                                                                                                                                   aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGAt   >  5:587291/1‑151 (MQ=255)
                                                                                                                                                   aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGAt   >  4:884042/1‑151 (MQ=255)
                                                                                                                                                    ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGAtt  >  1:630948/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGATT  >  DH1/936146‑936443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑