Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2970AG40.0% 197.6 / 189.2 167intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (78/15);  new base G (56/6);  total (134/33)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01

Only 100 of 172 total aligned reads displayed.

ACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGATTTGGC  >  DH1/936159‑936447
                                                                                                                                           |                                                                                                                                                       
                                                        cacgacCTTCGGGTTATGAGCCCTAGGAGCTTGCAGTCCTGACCTCGGCTCTTCAGCTTACTGC‑TTTAATTCATCAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                                                     <  7:110237/150‑1 (MQ=11)
                                                              cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGTAGTCG‑GTTACTTCATCAAATTTTAATTGATTGCGGGTGGGTGATTTGAACCTCAGACCTTAGGGTTATGATACAGACGAGCTAACACGCTGCTCCAc                                                                                >  2:1300518/1‑150 (MQ=11)
                                                                                acgaGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATgcgc                                                             >  3:635865/1‑151 (MQ=255)
                                                                                  gaGCTACCAGGCTGCTCCACCCCGCGTCCCCGTAGTGC‑GTTAATTCATCAAAGTTTATTTGGTTCCGGGGTCAGGATTTGACCCGCCCCCCTTCGGGTTTAGAGGCCGACTAGCTACAACGCTACTCCCGACCGCGTCCGTCGATgatccg                                                           >  7:720225/1‑146 (MQ=9)
                                                                                          aGGCTGCTCCACCCCGCGTCCCGGTAGGGC‑GTTTCTTCATCAAATTTTAATTGGTTCCGCGGGCCGGATTTGCACCGAACCCCTTCGGGTTATGAGACCGACGAGCGACCAGGCTGCTGCCCACAGAGTCCGTGGATgatccgtaaagtcc                                                   >  2:167278/1‑138 (MQ=9)
                                                                                             ctgctCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGTGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCt                                                >  6:1669693/1‑151 (MQ=255)
                                                                                                     cctcgcgTCACCGTACTGC‑TTTAACTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                                        <  3:64745/148‑1 (MQ=255)
                                                                                                     ccccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                                        >  3:1623406/1‑151 (MQ=255)
                                                                                                      cccTCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCCCTcgcg                                            >  3:1846999/1‑146 (MQ=11)
                                                                                                      cccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAGTATACTACGCTCGCGTTTTc                                       >  3:1207895/1‑151 (MQ=11)
                                                                                                       ccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCCGGCTGCTCCACCCCGCGTCCGGGGATGCGGACTCTACTCCGCTCTCTttttgc                                      >  1:888478/1‑149 (MQ=11)
                                                                                                        cgcgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTACGCTAGAGttttagt                                     >  4:64711/1‑148 (MQ=11)
                                                                                                        cgcgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACTCCGCTCGATATTTCAt                                     >  1:107853/1‑151 (MQ=11)
                                                                                                        cgcgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGAACTATACTGCGCTAGAGATTTCAt                                     >  8:396932/1‑151 (MQ=11)
                                                                                                         gcgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGGGGAGTATACTGCGCTGGAGTTTTGATg                                    >  7:1460414/1‑151 (MQ=11)
                                                                                                          cgTCACCGTACTGG‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGGTTTCATGc                                   >  4:1332665/1‑151 (MQ=255)
                                                                                                          cgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTATCTATTTCATGc                                   >  2:135943/1‑151 (MQ=11)
                                                                                                          cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                   >  7:1165375/1‑151 (MQ=255)
                                                                                                          cgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                   >  1:1259622/1‑151 (MQ=255)
                                                                                                           gTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAGTATACTCCGCTAGGGTTTTCATGc                                   >  1:1868508/1‑150 (MQ=11)
                                                                                                           gTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAAGCGGACTATACTACGCTAGAGAATTCATGc                                   >  2:678396/1‑150 (MQ=11)
                                                                                                           gTCACCGTACTGC‑TTTTAGTCATGAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                                  <  7:397279/151‑1 (MQ=255)
                                                                                                            tCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGGGGAGAATACTCCGCAAGGGTTTTCGTGccc                                 >  4:1739396/1‑149 (MQ=11)
                                                                                                            tCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAATCGCAAAATACTCCGCTAGAGTTTTCATGCac                                 >  2:285611/1‑150 (MQ=1)
                                                                                                            tCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAGTCGCAAAATACTCCGAAAGTGTATTCATGCac                                 >  2:796769/1‑150 (MQ=11)
                                                                                                             cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGGGGAGTATACTCCGATCGCGTTTTCATggacc                                >  5:1442361/1‑147 (MQ=11)
                                                                                                             cACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCCGTCAATACTCCCCACGAGTATTCATGCCAc                                >  1:463702/1‑151 (MQ=11)
                                                                                                             cACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGCCGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                                >  2:870861/1‑151 (MQ=255)
                                                                                                              aCCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                               >  1:350849/1‑151 (MQ=255)
                                                                                                               ccGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAAGCGCACAATACAACACACGAGAATTCATGCAACCt                              >  4:1449494/1‑151 (MQ=11)
                                                                                                               ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                              >  7:376836/1‑151 (MQ=255)
                                                                                                                cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGGACTATACTACGCTATGGTTTTCATGCAACCtt                             >  4:370923/1‑151 (MQ=11)
                                                                                                                  tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGCTGTATACTACGATCGCGTTTTCATTCAACCttt‑t                          >  3:1731308/1‑151 (MQ=14)
                                                                                                                   attccgttctcTTCATAAAATTTT‑ATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑t                          <  6:819928/140‑1 (MQ=18)
                                                                                                                   aCTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGAAGAGCTACCAGGCTGATCCACCCCGCGTCCGTGTATGCGCACAATACTACGATCTCGTTTTCCTGCAAcgctatt                          >  2:285326/1‑145 (MQ=11)
                                                                                                                    cTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑ttt                        >  1:1212648/1‑151 (MQ=255)
                                                                                                                    cTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtgt‑ttt                        >  2:680597/1‑151 (MQ=255)
                                                                                                                     tgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACAATACTCCCATCGGGATTTCCTGCACCCgtg‑tttt                       >  7:1773540/5‑151 (MQ=11)
                                                                                                                     tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCttt‑tttt                       >  5:701592/1‑151 (MQ=255)
                                                                                                                      gcttttACTTC‑TTAAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                                    <  6:841887/135‑1 (MQ=17)
                                                                                                                      gC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑ttttt                      >  2:1822733/1‑151 (MQ=255)
                                                                                                                       c‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGACCGTGGATGCGCACTATACTCTGCTCGCGTTTTCATGCAACCtgt‑ttttt                      >  8:1257099/1‑150 (MQ=255)
                                                                                                                       c‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCTGCTACACCCCGCGTACGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt‑ttttt                      >  1:1304139/1‑150 (MQ=255)
                                                                                                                        ttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCCACTATACTCCGCTATCGATTTCATGCAACCTTT‑TTTTTa                     >  6:1376989/2‑150 (MQ=17)
                                                                                                                        ttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTATGGGCAGTATACTCCGATCGAGTTTTCCTGGACCGTTG‑TGTTTAc                    >  7:1266031/2‑151 (MQ=11)
                                                                                                                         tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAc                    >  5:1175850/1‑151 (MQ=255)
                                                                                                                         tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAc                    >  6:1777669/1‑151 (MQ=255)
                                                                                                                         tttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACAATACTCCACTCTATATTTCATGCAACCt                              >  8:1857343/1‑141 (MQ=14)
                                                                                                                         tttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGAAATATTCTCCGATAGAGTATTCATGCAACCGTT‑TTTTTaac                   >  6:961335/1‑149 (MQ=14)
                                                                                                                          ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAcc                   >  7:501187/1‑151 (MQ=255)
                                                                                                                          ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGGGGTATACTTCGCTGTGGTTTTCTTTGAGCCttt‑tgtataacc                  >  4:977401/1‑143 (MQ=11)
                                                                                                                           aaCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAccc                  >  5:230856/2‑151 (MQ=255)
                                                                                                                            tactcatacaaTTTTTATTGGTTGCGGGGGCCGGATTTCCACCGACGTCCTTCGGGATATCATCCCGACGAGGTACCAGGCTCCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAccc                  <  1:1857130/141‑1 (MQ=21)
                                                                                                                            aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAcccc                 >  1:1555752/1‑151 (MQ=255)
                                                                                                                            aCTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGCAGTATACTACACTCGCTTTTTCGTTCAACCTGG‑TTTTTaacacc                >  6:1058568/1‑146 (MQ=14)
                                                                                                                            aCTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCACGACGAGCTACCAGGCTGCTCCACCCAGCGTCAGTCGATGCGCAATATACAACACTCGCGATTTCATGCAACCTTTATTTTTAccac                 >  1:706982/1‑149 (MQ=14)
                                                                                                                             cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGAGGCCCTTCGGGTTATCAGCCCGACGCCCTCCCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCTTT‑TTTTTACCCCg                >  4:1081308/1‑151 (MQ=25)
                                                                                                                             cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCg                >  8:19856/1‑151 (MQ=255)
                                                                                                                             cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCTATATACTACGCTAGCTTATTCCTTCAGCCtgt‑tagatgggaacc               >  5:820900/1‑140 (MQ=11)
                                                                                                                             cTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAAGCGCACTATACTCCGCTAGAGTATTCATGCAACCTTA‑TTTTTAacccc                >  2:1712204/1‑149 (MQ=17)
                                                                                                                              ttCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGGACTATACTCCTCTCTCTATTTCATTCAACCGTG‑TTTTTAccacc                >  3:61406/1‑146 (MQ=14)
                                                                                                                              ttCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGGCAAGTATACACCCATAGATTTTTCATGCACCCTTT‑TTTTTAccccc                >  2:1735311/1‑148 (MQ=11)
                                                                                                                              ttCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGAACTATACTCCGCTAGCTTTTTCATTCAACCGTT‑GTTTTACCCCCAt              >  6:1038283/1‑151 (MQ=17)
                                                                                                                              tagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCTTTTTCATGCAACCTTT‑TTTTTag                    >  8:1453773/13‑145 (MQ=18)
                                                                                                                              tagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCg                <  6:448492/138‑1 (MQ=255)
                                                                                                                              tagggggttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTAc                    <  7:1455200/134‑1 (MQ=255)
                                                                                                                               tggttgttaaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGa               <  4:1140842/139‑1 (MQ=255)
                                                                                                                                catcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGCGGATTCGGAGTCTACAACACTCTATTTTTGATGCAACCTGA‑TTTATAACCCGagcc            >  7:771749/1‑148 (MQ=0)
                                                                                                                                attcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCCTT‑TTTTTACCCCGATc             <  1:285406/148‑1 (MQ=255)
                                                                                                                                acataaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc             <  3:1451035/147‑1 (MQ=255)
                                                                                                                                 atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTGGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc             >  1:792392/1‑150 (MQ=255)
                                                                                                                                 atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc             >  1:689528/1‑150 (MQ=255)
                                                                                                                                 atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATc             >  3:43052/1‑150 (MQ=255)
                                                                                                                                 atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCAc           >  5:346617/1‑151 (MQ=255)
                                                                                                                                  tcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACTGGAAGCGAACTATACTACACTAGAGTATTCATTCAACCTTA‑ATTTTAACACCATCAcc          >  6:237139/1‑150 (MQ=11)
                                                                                                                                    caaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCTTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg         >  4:1778074/5‑151 (MQ=255)
                                                                                                                                    caaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAAGCGAACTATACTCCTAAGGATAATTCATTCAACCTTT‑TTTTTAcaaccaacacgc         >  8:883119/5‑140 (MQ=11)
                                                                                                                                    aaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg         <  4:764175/151‑1 (MQ=255)
                                                                                                                                    aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg         >  3:1178960/1‑151 (MQ=255)
                                                                                                                                    aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCAACCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg         >  2:1933019/1‑151 (MQ=255)
                                                                                                                                     ttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATAc                                                      <  1:1793030/101‑1 (MQ=255)
                                                                                                                                     ttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCa            <  3:19248/142‑1 (MQ=255)
                                                                                                                                     ttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCGCTAGAGTTTTCATGCAACCTTT‑ATTTTAACCCGATCaaga         >  4:19203/6‑147 (MQ=18)
                                                                                                                                     aaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGGTCTATACTCCGCTCTCTTTTTCCTGCACCCTTT‑TTTTTACCCCGATCACgg         >  1:1074884/4‑150 (MQ=18)
                                                                                                                                     aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg         >  7:653811/1‑150 (MQ=255)
                                                                                                                                     aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACgg         >  6:83145/1‑150 (MQ=255)
                                                                                                                                      aatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGAt       <  5:1039602/149‑1 (MQ=255)
                                                                                                                                      aatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACACCGCACGCGTTTTCATGCAACCTTT‑TTTTTAaccccagcaag          >  2:1112333/3‑141 (MQ=18)
                                                                                                                                      aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGAt       >  4:884042/1‑151 (MQ=255)
                                                                                                                                       ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGAtt      >  1:630948/1‑151 (MQ=255)
                                                                                                                                       caatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGCACTATACTCCGCTCGCGTTTTCATTCAACCTTT‑TTTTTACCCCGATCACGCAtt      >  5:621484/4‑151 (MQ=255)
                                                                                                                                       atttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGGGGAGTATACTCCACTCGCTTTTTCATGCACCCTTA‑GTTTTAaccacagacgccatt      >  2:455660/2‑139 (MQ=14)
                                                                                                                                         atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCaa                                 <  8:1297922/122‑1 (MQ=255)
                                                                                                                                          tAATTGGTTGCGGGGGGGGGATTTGAACCGAGGACCTTCGGGTTATGAGCCCGACGAGCTTCGAGGCTTCTCCTCCGCTCGTCCTTGGATGGGCACTTTTCTGCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACGCCTATAACGGTTTTg    <  8:1106106/150‑1 (MQ=18)
                                                                                                                                          tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAt                                                                 >  4:1452855/1‑90 (MQ=38)
                                                                                                                                          tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAt                                                                 <  3:1454387/90‑1 (MQ=38)
                                                                                                                                          tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTAT‑TTTTTACCCCGATCACGGATTTgg   >  3:1355497/1‑151 (MQ=255)
                                                                                                                                           aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCg                                                  >  8:636346/1‑104 (MQ=255)
                                                                                                                                           aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcg                                              >  5:573575/1‑108 (MQ=255)
                                                                                                                                           aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcg                                              <  6:572933/108‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                       
ACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTT‑TTTTTACCCCGATCACGGATTTGGC  >  DH1/936159‑936447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑