Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6050AT31.1% 308.6 / 148.5 226intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (103/52);  new base T (49/21);  total (152/74)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

ATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065455‑1065754
                                                                                                                                                         |                                                                                                                                                      
       aaTCTGCAGTAAAGTAAGTTGCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑GCGGAGGTTCGAATCCTCCCGGATTCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTaa                                                                                                                                                 <  7:571696/151‑1 (MQ=255)
               gtaaagtaaGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTaaaaatatcg                                                                                                                                         >  3:1770680/1‑145 (MQ=255)
                         tttCGCGATGCATCCGGAGCTCATCTGGAGAGAGGAATCGGCTACGAACCGTTGGG‑TCGGCGCTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGc                                                                                                                                <  5:1528364/150‑1 (MQ=18)
                         tatCCCTATGCATCCGTAGCTCATCTTGATAGAGTACTCGGCTACGAAGGGTTCGG‑TCGTAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGc                                                                                                                                <  8:1080059/148‑1 (MQ=21)
                                    aTCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAGTTTGCAGAtcgctagctaacacg                                                                                                                    >  7:746856/1‑137 (MQ=21)
                                        gTAGCGCAGCTGTATAGAGTACTCGGCTCCGAACGGCGCGG‑TCGGCGGTTCGAATCCTCCCGGCTGCACCATCTCTTACTTGATAGGGCTTTAGTAGCGATCTC‑‑AATATCAGCAGTAAACTAAAATTCCCGATGCATCCGTAGCTCAGCTg                                                                                                                <  5:1861447/151‑1 (MQ=21)
                                          cgCTCAGCTGCAGAGAGCACTCGGCGACGACCCGCGCGG‑TCGTATTTTTTATTAATTGATCCTGCAACATCTCTTACTTTATCTGGCTATTTTAGTGTTATC‑‑AATATCTTCTGTAATATTATTTTCCATATGCATCCGTAGCTCAGCTGGa                                                                                                              <  4:1571950/150‑1 (MQ=255)
                                           gCTCAGCTGGATAGAGTACTCGGCTACTTTTTTATAGG‑TTGGTGGTTCGAATGATAGCTGATGCTCCATATCATATTTTATATTTCTATACTATCTTTATC‑‑AATATCAGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGAt                                                                                                             <  8:1180044/151‑1 (MQ=9)
                                              aaGCTGGATATTCTACTCGGCTACGATCGCTTCGG‑TCGGAGCTTCTAATCCTCCCTGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATaga                                                                                                          <  1:1473566/150‑1 (MQ=17)
                                               aGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATCTTGCTGTAGGAGCGGAAGC‑‑AATATCAGCAGTAAATTAAATATCCCGATTCTTCCGTTCCGCAGCTGtatacat                                                                                                         >  7:710564/1‑148 (MQ=18)
                                                   ggtttttctACTCGGCGACTAG‑CGAGCGGCTCGGCGCGTCTAATCCTCGCGGATTCAACATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACt                                                                                                     <  2:116694/143‑1 (MQ=14)
                                                   ggATAGTTTAATCGGCTACTATCGGTTCGG‑TCGGCGGGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTa                                                                                                       <  5:550331/149‑1 (MQ=14)
                                                     gtatactACTCGGCGACTAGCGGTCCGC‑TCGGCGCTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCg                                                                                                   <  1:825985/145‑1 (MQ=14)
                                                                ggCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACGTTATATGGATATAGGAGCGGTAGC‑‑AATATCATCAGTAAAAAAAAGTTCCCGATGCATAAGAATCACATCGGGATAGAGGTCTCCGCTACgcgcgc                                                                                        >  6:730185/1‑146 (MQ=12)
                                                                   gacgagggcgaggg‑TCGGCGAGTCTAATCCTCGCTTATTCACCATCTCTTACTTGATATGGCTTTAGTAGGGGTATC‑‑AATATCAGCAGTAAACTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGc                                                                                     <  3:1090376/139‑1 (MQ=14)
                                                                    aCGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACGTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGCTGCATCCGTAGCTCAGCGGGATAGAGTACTCGGCTACGACCCCAGCg                                                                                    >  3:1911374/1‑151 (MQ=255)
                                                                    aCGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTAGTACTTAATATGGAGGTATTAGCGTAAAC‑‑AATATCAGCAGTAATATAAATAACGCGATGCAGAAGAAGAACATCTTGATAGAGTACTCGGCTAGGAGCcgcgcg                                                                                    >  8:245903/1‑151 (MQ=12)
                                                                       accggggcgc‑TCGGAGCGTCTAATGCTCCCTGATGCACCATCTCTTACTTGATATGTCTTTAGTAGCGGTATA‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑Tc                                                                                <  3:484388/145‑1 (MQ=14)
                                                                       aaCCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTAGATATGGCGCTAGTAGGGGTTGC‑‑AATATCAAGAGTAAAATAAAGATCCCGATGCGTAGGTAGCGCTTCTGGATGGAGAACTCGGATACGACCCCAGCCG‑Tc                                                                                >  5:1369380/1‑151 (MQ=12)
                                                                         ccgctcGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTagaa                                                                                                                                               <  1:1772870/82‑1 (MQ=14)
                                                                         ccGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTagaa                                                                                                                                               >  2:1771074/1‑87 (MQ=255)
                                                                          cGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATGATACTTGATATTGATCTAGGAGCGGATGG‑‑AATATCTGGAGGAAATTAACTAACCCGCTCCGGCGGTATATCAGCTGGAGAGCGTCCTCGGCTGCGAGACGggcgg‑gcgc                                                                              >  4:1442421/1‑149 (MQ=2)
                                                                           gAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATCTTACTTGATATGGCTTTAGTAGCGGTTTC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCCTCCGTAGCTCAGGTGGATACAGTACTCGGCTACGCCCCGAGCCG‑TCgg                                                                              >  7:211660/1‑149 (MQ=17)
                                                                            aGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTTTACTTGATATTGCGCTATGAGGGGTTGC‑‑AATATCTGCAGTAAAATAACTTTCCCGATGCGTAGGTAGAGCAGCTGGTTAGAGAGCTAGGGTACGAGACGAGCGG‑TCGGAgg                                                                           >  1:1905167/1‑151 (MQ=12)
                                                                               ggctcggcgcgtctaaTCCTCGCGGATGCACCATCTTTTAGTTGATATGGCTTTAGTAGCTGTATA‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGtt                                                                         <  8:1500856/137‑1 (MQ=11)
                                                                                gggaggAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATA‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTc                                                                        <  7:737935/147‑1 (MQ=255)
                                                                                 gtcggcgcgtCGAATCCTCGCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCg                                                                       <  4:1600732/142‑1 (MQ=255)
                                                                                   cggcgcgtctaaTCCTCCCGGACTCACCATCTCTTACTTGATATGGCTTTACTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGaa                                                                     <  1:1070629/141‑1 (MQ=14)
                                                                                        gTTCGAATCCTCCCGGATGCACCATATGGTACTTGATATGCATGTATTAGCGGTTGG‑‑AATATCAGCAGTAAAATAAATTTCCCGATTACAACGTAGCACAGCTGGAGAGAGTTCTAGGCAACGAAACCAGCGG‑TCGGAGGGTCGATtccgc                                                                >  7:1225337/1‑149 (MQ=255)
                                                                                          tctaatgctcgggtaTGCAACATCTCTTACTTTATCTGGCTTTAGTAGCGGTATA‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAAtcctcc                                                               <  1:1044277/136‑1 (MQ=14)
                                                                                           cGAATCCTCCCGGATGCACCATCTATTACTGGATATCGCGTAATTAAAGCTAAC‑‑AATATCATCAGTAAAATAAATTTCCCGATGAATCAGTAGCTCAGCAGGATAGAGAACTCGGCTACGCTCCGAGCCG‑TCGGAGGTTACAAtccgcagg                                                             >  4:359265/1‑146 (MQ=9)
                                                                                            gAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTaga                                                                                                                                                >  2:215506/1‑66 (MQ=17)
                                                                                             actccccccctccccACCATCTCTTACTATATCTGGCTATACTAGCTTTATA‑‑AATATCAGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCgg                                                            <  6:535955/136‑1 (MQ=11)
                                                                                             aaTCCTCCCGGACGCACCATCTCTAACTTGATCGGGCTATACTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGa                                                           <  5:1616427/151‑1 (MQ=14)
                                                                                                cctccCGGATGCACCATCTCATACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATCCATCCGTAGCTCAGCTGGATAGAGTACTCCGCTACGAACCGAGCGC‑TCGGAGGTTCGAATCCTCCCGGATGc                                                        >  5:1836948/1‑151 (MQ=255)
                                                                                                  tccCGGATGCACCATCTATTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTACCGATGCATCCGTAGCTCAGCTGGATAAAGTACTCGGCAAACAAACGAGCCG‑TCGGAGGTTCGAATCCGACCGGATGCAc                                                      >  2:576901/1‑151 (MQ=14)
                                                                                                      ggATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCCCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCat                                                   >  3:255757/1‑150 (MQ=255)
                                                                                                       gctccACCATCTCATACTTGACATCGCTATTCTAGCGGTATC‑‑AAAATCAGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCatat                                                 <  1:451408/147‑1 (MQ=11)
                                                                                                         ttctatatctgatACTTGATCTCTCTTTATTATCTCTTTC‑‑AAAATCAGTATTAAATTAAATTTCCCGATTCATATGTAGCTTAGCTTTATTGAGTAATCGGCTAGTATCCGTTGGG‑TCTTTGGTTCTAATCCTCCCGGATGCACCATAtt                                                <  4:951816/138‑1 (MQ=9)
                                                                                                         tGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATtc                                               >  7:103710/1‑151 (MQ=39)
                                                                                                          gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCGGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                              >  1:599518/1‑151 (MQ=255)
                                                                                                          gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATtct                                              >  8:206686/1‑151 (MQ=39)
                                                                                                           cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                             >  4:526157/1‑151 (MQ=39)
                                                                                                           cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                             >  3:1460301/1‑151 (MQ=39)
                                                                                                            aCCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTcc                                            >  3:411488/1‑151 (MQ=39)
                                                                                                              cATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCg                                           >  2:33082/1‑150 (MQ=40)
                                                                                                               aTCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAAATTCCCGATGCATCCGTAGCTCAGCAGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGa                                          >  6:1424202/1‑150 (MQ=255)
                                                                                                                tctcTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATcagca                                                                                                                                                     >  3:1314195/1‑43 (MQ=35)
                                                                                                                 ctctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAt                                       >  8:683452/1‑151 (MQ=255)
                                                                                                                 ctctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAt                                       >  6:655837/1‑151 (MQ=255)
                                                                                                                 ctctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAt                                       >  2:1286496/1‑151 (MQ=255)
                                                                                                                   ctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTACCGATGCATACCTAGCTCAGCTGGATAGAGTACTAGGCTACGAACCGAGCCG‑TCGGAGGTTCGAATCCTCCCCGATGCACCataatatacgtaaaa                                      >  4:610014/1‑138 (MQ=14)
                                                                                                                    ttACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCtt                                    >  5:1407009/1‑151 (MQ=255)
                                                                                                                      aCTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGCTGCACCATATTCTCCGAAATCTTc                                   >  8:332146/1‑150 (MQ=255)
                                                                                                                        ttGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTc                                   >  8:648363/1‑148 (MQ=255)
                                                                                                                         tGATACGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATCTTcct                                 >  2:994989/1‑149 (MQ=14)
                                                                                                                           atatGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAAt                             >  8:1039128/1‑151 (MQ=255)
                                                                                                                           atatGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAAt                             >  7:1318764/1‑151 (MQ=255)
                                                                                                                           atacGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAGGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAGATCTTccga                                >  2:95453/1‑147 (MQ=14)
                                                                                                                           atacGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATACTcctatatatggata                                >  5:371655/1‑138 (MQ=14)
                                                                                                                            tacggCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATCTTCataa                               >  7:172097/4‑147 (MQ=14)
                                                                                                                            tacggCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATACTACTAAATATTCatga                               >  8:843415/4‑147 (MQ=14)
                                                                                                                             acggCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCCCCATCTAATACGTAATCTTcctctat                             >  7:633743/3‑145 (MQ=17)
                                                                                                                               ggCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                           >  4:203101/1‑149 (MQ=255)
                                                                                                                                gtttttgttgggttata‑‑AATATCAGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAgg                        <  6:918512/138‑1 (MQ=11)
                                                                                                                                gCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCTAGATATTCCTCAATGa                           >  6:1158883/1‑150 (MQ=14)
                                                                                                                                gCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATATTCAGCAATGa                           >  5:1650884/1‑150 (MQ=11)
                                                                                                                                gCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAgg                        >  8:576647/1‑151 (MQ=255)
                                                                                                                                 cTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGAAGGt                       >  3:939009/1‑151 (MQ=25)
                                                                                                                                  tttAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCCGCAATGAAgg                        >  3:1800762/1‑151 (MQ=11)
                                                                                                                                  tttAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACAGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGAAATATTCAGAAAttagcg                        >  8:1532995/1‑146 (MQ=14)
                                                                                                                                   ttAGTAGCGGTTTCTAAAAATCTGCAGTAAATTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGaa                          <  2:281201/148‑1 (MQ=11)
                                                                                                                                   ttAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCTAAATAttgctgtattatggt                       >  3:1728838/1‑138 (MQ=14)
                                                                                                                                     attataggtttaaaaaTATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTa                      <  8:172008/136‑1 (MQ=11)
                                                                                                                                      ttataggtttctaaaTATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTAt                     <  8:1864655/137‑1 (MQ=11)
                                                                                                                                      gtagCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATATTCAGAAATTAACGTAt                     >  6:991548/1‑150 (MQ=11)
                                                                                                                                       tttaggtttaaaaaTATCTGCAGTAAAATAATTTTCCCGATGCATCCGTATCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATc                    <  2:82923/138‑1 (MQ=11)
                                                                                                                                       taggtttata‑‑AATATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                  <  6:34560/144‑1 (MQ=11)
                                                                                                                                       tagCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTACTAAATATTGAGGAATGAAGGTaaac                   >  7:1866683/1‑148 (MQ=14)
                                                                                                                                        cgagctttcaaaaaatCTGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCg                   <  1:996332/136‑1 (MQ=14)
                                                                                                                                          cgctctC‑‑AATATCAGCAGTAAAATAAATTTCCCTATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAagag              <  3:1408313/146‑1 (MQ=255)
                                                                                                                                           ggtttctaaaTATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGACGTTCGAATCCTCGCGGATGCACCATATTCTCCGAATTCTTCAGCAATGAAGGTATCGAaga               <  4:1213441/143‑1 (MQ=11)
                                                                                                                                           ggtatcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACTAAATATTCATCAATTAAGGTATCGa                  >  7:791891/12‑148 (MQ=11)
                                                                                                                                           ggtataaaaaaatCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAaga               <  8:791034/140‑1 (MQ=11)
                                                                                                                                             tatcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATTCAGAAATGAAGGTATAGatcgt              >  6:1820352/10‑146 (MQ=11)
                                                                                                                                              atcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAAGGAGGGTATCCAAGAGGt            >  1:1171554/9‑151 (MQ=14)
                                                                                                                                                c‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg        >  1:1574676/1‑151 (MQ=255)
                                                                                                                                                 taaaTATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCa                               <  5:704708/127‑1 (MQ=16)
                                                                                                                                                    atatCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTc                                                                        <  7:1533372/85‑1 (MQ=255)
                                                                                                                                                    aaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTCCGAAATATGCAGATATTAAGGTATCCAATATGTacagg       >  5:1189230/3‑146 (MQ=11)
                                                                                                                                                     tatCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGa                                                                             >  1:1281166/1‑79 (MQ=255)
                                                                                                                                                     aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATATTCATCTATGAAGGTATCGAAGATGTCGAGGa      >  7:1564192/2‑150 (MQ=11)
                                                                                                                                                     aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCGAAATCTTCATAAATGAAGGTTTCGaatatgatgagta      >  2:706151/2‑139 (MQ=11)
                                                                                                                                                     aatCAGCAGTAAACTCTCTTTCCCTATGTATCCGTAGCGCAGCTGGTTCGCGTCCTCGGCTA‑GTACGGCGAGGCGCTCTGGGAGTAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGaa     <  6:1082478/150‑1 (MQ=9)
                                                                                                                                                      atCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGCAATGAAGGTAACGAAGAggaaacataac    >  1:28571/1‑142 (MQ=14)
                                                                                                                                                      atCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGACATATTCAGGAATGAAGGGTTCGAAGATGTAGCGGAAt    >  1:728760/1‑151 (MQ=14)
                                                                                                                                                      atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAt    >  2:1610785/1‑151 (MQ=255)
                                                                                                                                                      atCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAt    >  3:741774/1‑151 (MQ=255)
                                                                                                                                                        cagcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt   >  7:348417/1‑150 (MQ=255)
                                                                                                                                                         agcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCgg                                                            >  1:905539/1‑92 (MQ=38)
                                                                                                                                                         |                                                                                                                                                      
ATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065455‑1065754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑