Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6180AG25.8% 395.6 / 157.3 263intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (119/48);  new base G (52/6);  total (175/88)
Fisher's exact test for biased strand distribution p-value = 4.15e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

Only 100 of 285 total aligned reads displayed.

AAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACTAG  >  DH1/1065477‑1065768
                                                                                                                                                |                                                                                                                                                      
         gATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTTGATATGCCTGTAGTAGGGGTATC‑‑AATATGACCAGTAAAATACATTTCGCCATGCATcaga                                                                                                                                       >  7:722127/1‑147 (MQ=17)
         gATGCAGCGGGAGGTCAGCTTGATAGACTACTCGGCGACTACCGGTAGGG‑GCTGAGGGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTa                                                                                                                                      <  7:349697/151‑1 (MQ=14)
          aTGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGAAAGGGTAAG‑‑AAGATCTTGAGTAAAAAAAATGTcgcgctcggtcggta                                                                                                                                      >  6:531546/1‑139 (MQ=17)
                    cgCTCAGCTGCAGAGAGCACTCGGCGACGACCCGCGCGG‑TCGTATTTTTTATTAATTGATCCTGCAACATCTCTTACTTTATCTGGCTATTTTAGTGTTATC‑‑AATATCTTCTGTAATATTATTTTCCATATGCATCCGTAGCTCAGCTGGa                                                                                                                           <  4:1571950/150‑1 (MQ=255)
                               atatAGTACTCTCCTACGATCTGATCCG‑TTGGAGCGTCTAATTCTCCCGGTTGCAACATCTCTTACTTGATATGGCTTTATTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCg                                                                                                                <  8:734726/151‑1 (MQ=14)
                                tttactACTCGGCGACG‑ACCGCGCGGCTCGGCGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATA‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCg                                                                                                                <  1:614969/145‑1 (MQ=14)
                                             gacgagggcgaggg‑TCGGCGAGTCTAATCCTCGCTTATTCACCATCTCTTACTTGATATGGCTTTAGTAGGGGTATC‑‑AATATCAGCAGTAAACTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGc                                                                                                  <  3:1090376/139‑1 (MQ=14)
                                              aCGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACGTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGCTGCATCCGTAGCTCAGCGGGATAGAGTACTCGGCTACGACCCCAGCg                                                                                                 >  3:1911374/1‑151 (MQ=255)
                                                 accggggcgc‑TCGGAGCGTCTAATGCTCCCTGATGCACCATCTCTTACTTGATATGTCTTTAGTAGCGGTATA‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTc                                                                                              <  3:484388/145‑1 (MQ=14)
                                                     gAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATCTTACTTGATATGGCTTTAGTAGCGGTTTC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCCTCCGTAGCTCAGGTGGATACAGTACTCGGCTACGCCCCGAGCCGTCgg                                                                                            >  7:211660/1‑149 (MQ=17)
                                                      aGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTTCCCATGCAACCCTAGCACAGCTGGATACAGTAAAAGGCTAAGCACCGAGCGGTcaccg                                                                                          >  7:17474/1‑146 (MQ=17)
                                                      aGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACTTGATATGGCTGTAGTAGGGGTTGC‑‑AATATCAGGAGTAATTTAAATGTGCCGATACATCGGTAGCAGATGTGGTTAGAGAAGTCGGCTCGGACCCCCGCGGTCCGAgg                                                                                         >  2:1906724/1‑151 (MQ=12)
                                                       gCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTTTTCCTAGATATGCATGTAGTAAGGGTAAG‑‑AAGATCTCCAGTAATATAAATATCGCGCTTCATAAGTAGCTCTGCGGGATACAGTAGTCGGCTACGAGCCCAGCGCTGCGAGGt                                                                                        >  5:1531796/1‑151 (MQ=12)
                                                          gggaggAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATA‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                                      <  7:737935/147‑1 (MQ=255)
                                                           gtcggcgcgtCGAATCCTCGCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCg                                                                                     <  4:1600732/142‑1 (MQ=255)
                                                                 gcgtctaaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcct                                                                               <  3:738338/145‑1 (MQ=255)
                                                                  gTTCGAATCCTCCCGGATGCACCATCTGGTACTTGATATGGCTGTAGTAGCGGTAGC‑‑AATATCAGCAGTAAAAAAAATGTCCCGCTGCATAAGTAGCACAGCGGGATAGAGAACTCGGCTACGAGCCGCGCGGGCGGAGGATCGCAtcctc                                                                              >  2:1561257/1‑151 (MQ=255)
                                                                  gTTCGAATCCTCCCGGATGCACCATATGGTACTTGATATGCATGTATTAGCGGTTGG‑‑AATATCAGCAGTAAAATAAATTTCCCGATTACAACGTAGCACAGCTGGAGAGAGTTCTAGGCAACGAAACCAGCGGTCGGAGGGTCGATtccgc                                                                              >  7:1225337/1‑149 (MQ=255)
                                                                   ttCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcctcc                                                                             >  5:1401904/1‑151 (MQ=255)
                                                                   ttCGAATCCTCCCGGATGCACCATATCTTACTTGATATGGCGTAATTAGCGGTTTC‑‑AATATCAGCATTAAAATAAATTTCCCGATGAATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGCTAGGAGCTTCGAAtcctcc                                                                             >  5:1468621/1‑151 (MQ=14)
                                                                       aaTCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCg                                                                           <  5:292612/149‑1 (MQ=40)
                                                                                   tGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATAtt                                                              >  8:1297404/1‑150 (MQ=40)
                                                                                     cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtctc                                                           >  4:526157/1‑151 (MQ=39)
                                                                                         aTCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGa                                                        >  1:1559226/1‑150 (MQ=40)
                                                                                             ctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACt                                                   >  7:752513/1‑151 (MQ=255)
                                                                                               tattttatatGTGTTTAGTAGCTGTCTC‑‑AATATCTGCAGTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGTATAGAGTAATCGGCTACGAACCGTTGGGTCTTTGGTTCTAATCCTCCCGGATGCACCATATTCTCCGAAATc                                                    <  8:1085478/142‑1 (MQ=14)
                                                                                                  ttGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGc                                              >  6:1091111/1‑151 (MQ=255)
                                                                                                  ttGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTc                                                 >  8:648363/1‑148 (MQ=255)
                                                                                                  ttGATACGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCtgcc                                                >  7:1599229/1‑148 (MQ=11)
                                                                                                  ttGATACGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTCCGACAtatgc                                                 >  3:1368/1‑146 (MQ=14)
                                                                                                    gATACGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATCtgccgg                                              >  3:1130814/1‑146 (MQ=14)
                                                                                                     atatGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAAt                                           >  8:1039128/1‑151 (MQ=255)
                                                                                                     atacGGCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGCACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGAAATATTCata                                              >  4:732911/1‑148 (MQ=14)
                                                                                                      tacggCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTACTAAATATTCatga                                             >  8:843415/4‑147 (MQ=14)
                                                                                                       atGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTGAGCTGGATAGCGTAGTCGGCTACGAAACGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCCCCAGATTGTCGGACATCTTCAGCAATgc                                         >  3:292822/1‑150 (MQ=14)
                                                                                                       acggCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCCCCATCTAATACGTAATCTTcctctat                                           >  7:633743/3‑145 (MQ=17)
                                                                                                         ggCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                                         >  4:203101/1‑149 (MQ=255)
                                                                                                          gCTTTAGTAGCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATATTCAGCAATGa                                         >  5:1650884/1‑150 (MQ=11)
                                                                                                          gCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAgg                                      >  8:576647/1‑151 (MQ=255)
                                                                                                            tttAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                                     >  2:421481/1‑150 (MQ=255)
                                                                                                             ttAGTAGCGGTTTCTAAAAATCTGCAGTAAATTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGaa                                        <  2:281201/148‑1 (MQ=11)
                                                                                                              tagtagCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCTGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATCTTAAGAAATGAAgct                                     >  8:288810/1‑148 (MQ=11)
                                                                                                              tagtagCGGTATC‑‑AATATCAGCAGTAAAATAAGATACCAACAGCATCCTTAGCTCAAATAGATAGATTACACGTCTACGAAACGCGGGGACGCAGATAAGAAAAAAAAAGGATGCACCATATTCTCCGAAATCGGGGGCAATGCAGGtggg                                  >  8:1874242/1‑148 (MQ=2)
                                                                                                              tagtagCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATc                                  >  6:541194/1‑151 (MQ=255)
                                                                                                                gtagCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAATATTCAGAAATTAACGTAt                                   >  6:991548/1‑150 (MQ=11)
                                                                                                                 taggtttata‑‑AATATCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                                <  6:34560/144‑1 (MQ=11)
                                                                                                                 tagCGGTATCAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTCCTACATATTCAGCAATGAAGGTAtac                                 >  2:986839/1‑149 (MQ=11)
                                                                                                                   gcgctctaaaaaaatCTGCAGTAACATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                               <  1:95699/138‑1 (MQ=14)
                                                                                                                    tatgttaaaaaTATCTGCAGTAAATTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                               <  4:48458/141‑1 (MQ=11)
                                                                                                                    cGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                              >  1:1053372/1‑149 (MQ=255)
                                                                                                                     ggtttctaaaTATCTGCAGTAAACTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGACGTTCGAATCCTCGCGGATGCACCATATTCTCCGAATTCTTCAGCAATGAAGGTATCGAaga                             <  4:1213441/143‑1 (MQ=11)
                                                                                                                     ggtataaaaaaatCTGCAGTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAaga                             <  8:791034/140‑1 (MQ=11)
                                                                                                                      gTATC‑‑AATATCAGCTTTAAAATAAATTTCCCGATGCATCAGTATCTCAGCTGTATAGAGTATTTGGCTACGAAACGATCTTTCGTTGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt                          <  6:1466837/151‑1 (MQ=14)
                                                                                                                        atcaaaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAAGGAGGGTATCCAAGAGGt                          >  1:1171554/9‑151 (MQ=14)
                                                                                                                         tC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCa                                             >  6:703843/1‑129 (MQ=18)
                                                                                                                          c‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg                      >  3:1778664/1‑151 (MQ=255)
                                                                                                                          c‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg                      >  3:1432621/1‑151 (MQ=255)
                                                                                                                           taaaTATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCa                                             <  5:704708/127‑1 (MQ=16)
                                                                                                                            gattttcttcagTAAATTAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGc                       <  4:931334/141‑1 (MQ=255)
                                                                                                                            aaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCCTGAATGAAGGTATCCAATAggaac                        >  8:245720/5‑146 (MQ=11)
                                                                                                                            aaaaatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGAAATTAACGTATAGAATATGTAg                        >  6:1211202/5‑149 (MQ=11)
                                                                                                                              attccgatattAAAGTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                     <  4:1674475/140‑1 (MQ=14)
                                                                                                                              atatCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                                      <  7:1533372/85‑1 (MQ=255)
                                                                                                                              atatCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                                      >  8:1531888/1‑85 (MQ=255)
                                                                                                                              atatCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                     >  6:1754646/1‑150 (MQ=255)
                                                                                                                              atatCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                     >  7:1340397/1‑150 (MQ=255)
                                                                                                                               tatCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGa                                                                                           >  1:1281166/1‑79 (MQ=255)
                                                                                                                               tatCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGa                                                                                           <  2:1279962/79‑1 (MQ=255)
                                                                                                                               tatCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGa                    >  5:1852817/1‑150 (MQ=255)
                                                                                                                               aatCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTAAATCTTCAGAAATGATGGTATACAAGAGCTacagga                    >  6:75842/2‑145 (MQ=11)
                                                                                                                                atCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGCAATGAAGGTAACGAAGAggaaacataac                  >  1:28571/1‑142 (MQ=14)
                                                                                                                                atCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGACATATTCAGGAATGAAGGGTTCGAAGATGTAGCGGAAt                  >  1:728760/1‑151 (MQ=14)
                                                                                                                                 tcttcagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGAAATATTCAGCAATGAAGGTATCGAAGAGCTAgacgatcc                 >  3:932619/5‑144 (MQ=11)
                                                                                                                                 tCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCAGAAATCTTCAGGAATGAACGTATCCAAGATCTAGCGGAttt                 >  2:933670/1‑151 (MQ=14)
                                                                                                                                  ctgcagTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTACTAAATATTCAGGAATTACGGTTTACAAGAGCTGGAGGaaat                 >  1:1036439/3‑148 (MQ=11)
                                                                                                                                  cagcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt                 >  2:1109574/1‑150 (MQ=255)
                                                                                                                                   agcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                                      <  4:993306/80‑1 (MQ=39)
                                                                                                                                   agcagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                                          >  1:905539/1‑92 (MQ=38)
                                                                                                                                    gcagTAAACTAACTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt                 <  5:1212747/148‑1 (MQ=255)
                                                                                                                                     cagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAAACTTCAGCAATGAAGGTATCGAAGAGATAgagaactga                >  8:1660196/1‑140 (MQ=11)
                                                                                                                                     cagTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTCGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATAATCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAAcc             >  1:476709/1‑151 (MQ=255)
                                                                                                                                      agtaaagtaaGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGAAATCTTCAGCAATGAGGGTATGGAAGAGGTAGCGGAATTaaa              >  4:1431578/1‑148 (MQ=14)
                                                                                                                                      agTAAAATAATTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCg            <  1:165370/151‑1 (MQ=255)
                                                                                                                                      agTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGACGGAGCTTCGAATCCTCCCGCATGAACCATATAATAAGAAATATTCACCAATGAAACTAAAGAAGAAGTAGCGGAATTAAca             >  8:354052/1‑149 (MQ=17)
                                                                                                                                       gTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTCTACTAAATATTCAGGTATGACGGTAAGCAAGAGCTCCAGGAATTAAcctg           >  8:174169/1‑149 (MQ=11)
                                                                                                                                        tAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAAATATTCAGCAATGAAGGTATGGAagaggaagaggatatcccaat           >  3:1676444/1‑141 (MQ=11)
                                                                                                                                        tAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATa                                                                                                                         >  2:807450/1‑40 (MQ=255)
                                                                                                                                        tAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc           >  6:1027387/1‑150 (MQ=255)
                                                                                                                                        tAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAGCTTCAGCAATGAAGGTATCGAAGAGATAGCGGTATTAAcc             >  1:1753583/1‑148 (MQ=16)
                                                                                                                                         taaataaacTACGCGATTCATATCTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCa          <  2:5424/149‑1 (MQ=14)
                                                                                                                                         aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCg                                                                                                     <  7:795342/59‑1 (MQ=40)
                                                                                                                                         aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc           >  6:189516/1‑149 (MQ=255)
                                                                                                                                         aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCa          <  5:39992/150‑1 (MQ=255)
                                                                                                                                         aaaaTAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGCAATGAAGGTATCGAAGAGATAGctccgaatagcgtcg         >  2:649567/1‑137 (MQ=11)
                                                                                                                                          aaataaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAgg        >  5:1689883/1‑151 (MQ=255)
                                                                                                                                            ataattTTCGCTATGAATCCGGAGCTCAGCTGGATAGAGTAGGCGTCTAGCATGCCCTCGGGCGGTGTTGCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCa      <  1:818666/151‑1 (MQ=14)
                                                                                                                                              aaatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTccc                                                                            <  3:325387/79‑1 (MQ=33)
                                                                                                                                               aatTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                                          <  7:1272778/80‑1 (MQ=32)
                                                                                                                                               aatTTCCCGAAGCATCTGTAGCTCTGCTTGTTATAGTTATCGGATAAGTACGGTGTTGGGTTATGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACTa   <  8:1224138/151‑1 (MQ=9)
                                                                                                                                                atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACTAg  <  1:1869119/151‑1 (MQ=40)
                                                                                                                                                |                                                                                                                                                      
AAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGG‑TCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AATATCAGCAGTAAAATAAATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACTAG  >  DH1/1065477‑1065768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑