Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,993,5330AG69.1% 747.4 / inf 492L55S (TTG→TCG) rcdADNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (82/70);  new base G (176/164);  total (258/234)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-03

Only 100 of 502 total aligned reads displayed.

AGCTCCATGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGCGGATCG  >  DH1/2993383‑2993682
                                                                                                                                                      |                                                                                                                                                     
 gCTCCATGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTaacaa                                                                                                                                                      <  4:423103/151‑1 (MQ=255)
    ccATGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCAGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACt                                                                                                                                                    <  6:1383625/150‑1 (MQ=255)
       tGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTCATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACtcatc                                                                                                                                                >  3:1393608/1‑151 (MQ=255)
        gTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACtcatc                                                                                                                                                >  6:1633320/1‑150 (MQ=255)
          tATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAAt                                                                                                                                             >  7:1435581/1‑151 (MQ=255)
           aTCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAATATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAAtt                                                                                                                                            <  8:1151335/151‑1 (MQ=255)
           aTCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAAtt                                                                                                                                            <  8:1363621/151‑1 (MQ=255)
             gcGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTc                                                                                                                                           <  2:1348144/149‑1 (MQ=255)
              cGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCt                                                                                                                                         <  2:882898/151‑1 (MQ=255)
               ggCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTg                                                                                                                                        <  6:1765909/151‑1 (MQ=255)
                gCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCT‑AAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGaa                                                                                                                                      <  3:92388/151‑1 (MQ=255)
                 ggTTGCAACCTGTGTGCTGTAGATCATATGGGTGATAGCCTGGCATGCGCCCGGAGGATGACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGaa                                                                                                                                      <  6:1249941/150‑1 (MQ=255)
                 cGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGaa                                                                                                                                      <  7:1554208/151‑1 (MQ=255)
                   ttGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGaaa                                                                                                                                     >  3:603297/1‑150 (MQ=255)
                    tGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGaaaa                                                                                                                                    <  3:351700/150‑1 (MQ=255)
                     gCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGaaaaa                                                                                                                                   <  7:325036/150‑1 (MQ=255)
                     gCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAAt                                                                                                                                  >  3:1700649/1‑151 (MQ=255)
                     gCAACCTGTGAGCTGTACATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATCATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCAGAAAAAt                                                                                                                                  >  8:1665570/1‑151 (MQ=255)
                      cAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAAta                                                                                                                                 <  5:115444/151‑1 (MQ=255)
                         ccTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAAtagta                                                                                                                              <  3:1279246/151‑1 (MQ=255)
                         ccTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAAtagta                                                                                                                              <  3:1048519/151‑1 (MQ=255)
                           tgtgAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAgg                                                                                                                            >  3:1211084/1‑151 (MQ=255)
                            gtgAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAAtagtag                                                                                                                             <  3:1083334/149‑1 (MQ=255)
                            gtgAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGt                                                                                                                           >  1:1720900/1‑151 (MQ=255)
                            gtgAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCAGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAAtagtag                                                                                                                             <  1:1004955/149‑1 (MQ=255)
                              gAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTc                                                                                                                          <  7:722482/150‑1 (MQ=255)
                              gAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCa                                                                                                                         <  7:78362/151‑1 (MQ=255)
                               aGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCAt                                                                                                                        <  5:1020984/151‑1 (MQ=255)
                                  tGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCg                                                                                                                      >  7:1023226/1‑150 (MQ=255)
                                   gTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGAt                                                                                                                    <  2:499912/151‑1 (MQ=255)
                                   gTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGAt                                                                                                                    <  1:70796/151‑1 (MQ=255)
                                   gTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGa                                                                                                                     <  5:1783021/150‑1 (MQ=255)
                                       aTCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATccc                                                                                                                 >  6:433953/1‑150 (MQ=255)
                                        tCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCa                                                                                                                >  7:1742421/1‑150 (MQ=255)
                                         cATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAACCTGCTGAACGCCTCCAGTACCAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAAc                                                                                                              >  2:780439/1‑151 (MQ=255)
                                         cATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAAc                                                                                                              <  2:236348/151‑1 (MQ=255)
                                         cATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAAc                                                                                                              >  1:133709/1‑151 (MQ=255)
                                            atCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGt                                                                                                           >  7:554401/1‑151 (MQ=255)
                                             tCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGt                                                                                                           >  5:1105452/1‑150 (MQ=255)
                                                   aTAGCCTGGCATGCGCCCGGAGCATCACTAAGATCGCTAAATAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAATAATAGTAGGTCATCGATCCCAACGGTACCCCgg                                                                                                    <  6:956963/151‑1 (MQ=255)
                                                         tGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCCATCCCAACGGTACCCCGGCAAggg                                                                                              >  8:1934551/1‑151 (MQ=255)
                                                         gggCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAggg                                                                                              <  4:1547333/150‑1 (MQ=255)
                                                          ggCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGt                                                                                             <  8:549846/151‑1 (MQ=255)
                                                             aTGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGc                                                                                          >  4:1910289/1‑151 (MQ=255)
                                                               gcgcCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCa                                                                                         <  4:1641490/150‑1 (MQ=255)
                                                                 gcCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAAtt                                                                                      <  1:212747/151‑1 (MQ=255)
                                                                 gcCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAAtt                                                                                      <  1:1593793/151‑1 (MQ=255)
                                                                   ccGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCATtttt                                                                                    <  8:749035/151‑1 (MQ=255)
                                                                   ccGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAAtttt                                                                                    <  8:106139/151‑1 (MQ=255)
                                                                      gAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCg                                                                                 >  6:86801/1‑151 (MQ=255)
                                                                       aGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCgg                                                                                <  7:1193935/151‑1 (MQ=255)
                                                                        gCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGt                                                                               >  5:1674755/1‑151 (MQ=255)
                                                                          aTCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGc                                                                             <  5:578569/151‑1 (MQ=255)
                                                                           tCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCg                                                                            >  7:1477677/1‑151 (MQ=255)
                                                                            cACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGt                                                                           >  5:933078/1‑151 (MQ=255)
                                                                             aCTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGt                                                                           >  1:935046/1‑150 (MQ=255)
                                                                             aCTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGt                                                                           >  3:1894659/1‑150 (MQ=255)
                                                                             aCTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTa                                                                          <  5:1929065/151‑1 (MQ=255)
                                                                              cTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGT‑AAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAAc                                                                        >  7:405224/1‑151 (MQ=255)
                                                                                aaCATCGCTAAAAAATGCCTGATATTGCCTGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACa                                                                       <  6:542884/151‑1 (MQ=255)
                                                                                  cATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGc                                                                     <  6:754545/151‑1 (MQ=255)
                                                                                     cGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCAt                                                                   <  3:1759703/150‑1 (MQ=255)
                                                                                      gCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGt                                                                 >  7:1304983/1‑151 (MQ=255)
                                                                                       cTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGt                                                                 >  7:1893034/1‑150 (MQ=255)
                                                                                       cTAAAAAATGCCTGATATCGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCTCAACGGTACCCCGGCAACCGTAGCAATTTTGCGGTGCGTAACAGCAAGt                                                                 >  1:1409035/1‑150 (MQ=255)
                                                                                           aaaaTGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTcc                                                            <  5:1433529/151‑1 (MQ=255)
                                                                                            aaaTGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCg                                                           <  2:1593923/151‑1 (MQ=255)
                                                                                            aaaTGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCg                                                           >  5:282646/1‑151 (MQ=255)
                                                                                                  cTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGt                                                     >  1:1183434/1‑151 (MQ=255)
                                                                                                    gATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGttt                                                   >  7:1123638/1‑151 (MQ=255)
                                                                                                    gATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGt                                                     <  1:839915/149‑1 (MQ=255)
                                                                                                           ccGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGcc                                            <  8:903380/151‑1 (MQ=255)
                                                                                                             gggACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTc                                          >  1:530154/1‑151 (MQ=255)
                                                                                                              ggACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTcc                                         >  2:682019/1‑151 (MQ=255)
                                                                                                              cgACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATACTAGGTCATCGATCCCAACGGTACCCCGGGAAGGGTAGCAATTATGAGGTGCGTAACAGCATGTATTCAGTAAAGTTTCACCGCCTcc                                         >  8:1243982/2‑151 (MQ=255)
                                                                                                               gtcATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGATAAATAGTAGGTCTTCGATCCGAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCa                                        <  8:821895/149‑1 (MQ=255)
                                                                                                                aCATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAg                                       <  7:1775280/151‑1 (MQ=255)
                                                                                                                 cATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGc                                      >  6:1125067/1‑151 (MQ=255)
                                                                                                                    gATCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTg                                   >  1:1504283/1‑151 (MQ=255)
                                                                                                                    gATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTg                                   >  7:964398/1‑151 (MQ=255)
                                                                                                                     aTCTCAGTAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTgg                                  >  5:415084/1‑151 (MQ=255)
                                                                                                                     aTCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTgg                                  >  4:1275286/1‑151 (MQ=255)
                                                                                                                         cAGTAAAACTGCTGATCGCCTCCCGTATCGACTCTTCAATTCCTGATAAATAGTAGGTCATCGATCCCAACGGTACCGCGGCAAGGGTAGCAATTTTGCGGTTGGTATCAGCTTGTATTGCGTAATGTTTCACCGCCTCCAGCGTGGCCt                               <  5:798406/150‑1 (MQ=255)
                                                                                                                         cAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTg                                   <  3:771957/146‑1 (MQ=255)
                                                                                                                         cAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTg                                   <  1:380029/146‑1 (MQ=255)
                                                                                                                         cAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTg                              >  4:756053/1‑151 (MQ=255)
                                                                                                                            tAAAACTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGa                            <  2:1804176/150‑1 (MQ=255)
                                                                                                                              aaaCTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGCGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATa                          >  1:1806757/1‑150 (MQ=255)
                                                                                                                                aCTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAAtt                       <  3:434439/151‑1 (MQ=255)
                                                                                                                                aCTGCTGAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAAtt                       >  4:1450595/1‑151 (MQ=255)
                                                                                                                                      gAACGCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAAtttttt                   <  8:355386/149‑1 (MQ=255)
                                                                                                                                          gCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGcc             >  8:1462266/1‑151 (MQ=255)
                                                                                                                                          gCCTCCAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGcc             <  8:44390/151‑1 (MQ=255)
                                                                                                                                          gCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGcc             >  4:1211217/1‑151 (MQ=255)
                                                                                                                                              gcAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGt           <  6:281469/148‑1 (MQ=255)
                                                                                                                                              ccAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTg         <  7:896737/151‑1 (MQ=255)
                                                                                                                                               cAGTAACGACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGc        >  8:1840417/1‑151 (MQ=255)
                                                                                                                                               cAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGc        <  4:1110545/151‑1 (MQ=255)
                                                                                                                                                aGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGCg       >  5:1309020/1‑151 (MQ=255)
                                                                                                                                                   aacgacTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGCGGa     <  4:54135/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AGCTCCATGTTATCCGGCGTTGCAACCTGTGAGCTGTAGATCATATCGGTGATAGCCTGGCATGCGCCCGGAGCATCACTAACATCGCTAAAAAATGCCTGATATTGCCGGGACATGATCTCAGTAAAACTGCTGAACGCCTCCAGTAACAACTCATCAATTCCTGAAAAATAGTAGGTCATCGATCCCAACGGTACCCCGGCAAGGGTAGCAATTTTGCGGTGCGTAACAGCATGTATTCCGTAAAGTTTCACCGCCTCCAGCGTGGCCTGGATAATTTTTTCTCGCCGTTGCGGATCG  >  DH1/2993383‑2993682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑