Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,448,5230AC24.8% 888.7 / 81.4 414intergenic (‑83/+73)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand):  ref base A (223/83);  new base C (0/101);  total (223/191)
Fisher's exact test for biased strand distribution p-value = 1.26e-44
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 445 total aligned reads displayed.

ATGAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAA  >  DH1/3448373‑3448673
                                                                                                                                                      |                                                                                                                                                      
aTGAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggagga                                                                                                                                                        <  3:1767699/151‑1 (MQ=255)
  gAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggagg                                                                                                                                                      >  5:246301/1‑151 (MQ=255)
  gAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggag                                                                                                                                                       <  1:62129/150‑1 (MQ=255)
   aGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggagg                                                                                                                                                      >  1:1655709/1‑150 (MQ=255)
      aGTTGATCTTTGCTTTTTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgg                                                                                                                                                  <  3:1424298/151‑1 (MQ=255)
      aGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGTGGAGGAGGACgg                                                                                                                                                  >  8:573376/1‑151 (MQ=255)
        ttGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgggg                                                                                                                                                >  5:649917/1‑151 (MQ=255)
         tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgggg                                                                                                                                                <  2:310096/150‑1 (MQ=255)
         tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTCATAACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggggc                                                                                                                                               >  1:556233/1‑150 (MQ=255)
           aTCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCg                                                                                                                                             >  2:548725/1‑151 (MQ=255)
           aTCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGAAGGACGGGGGCg                                                                                                                                             >  3:151295/1‑151 (MQ=255)
            tCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCACCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATATGGCGGAGGAGGACGGGGGCgg                                                                                                                                            >  1:1718536/1‑151 (MQ=255)
              tttgctttgTTTAACAAATGGGGGCCCCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTCAAGGATTTTTGGGGGCGGAGGCCGGGGGCGGa                                                                                                                                           <  2:600875/150‑1 (MQ=255)
               ttgtttttTTTAACAATTGGGGGCACGGGTAGGTGCCATTAATTTAAAATAATAATTCCCCTTTTATTAAAATCTGATCACCTTGCCATAGCATAATGCAGTATCACTCATCATCTTAATTATTTTTGGGGGCGGAGGCCGGGGGCGGAt                                                                                                                                          <  8:1462248/150‑1 (MQ=255)
               ttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCAATGCATTTAACATAATAATTCCCCTGTTATGAAAATCTTATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGAGGACGGGGGCgg                                                                                                                                            <  4:1044735/148‑1 (MQ=255)
                 gctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCAATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGGCGGGGGGGGATaa                                                                                                                                        >  2:1149826/1‑150 (MQ=255)
                  ctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGATAATCTGATCACCTTGCCATAGCCTAATGCAGGATCCCTCATCATCTGAAGGAAATTTGGGGGAGGAGGACGGGGGCGGATAACt                                                                                                                                      >  3:780363/1‑151 (MQ=255)
                      tttaaaCAAATGGGGGCACCGGCAGGTGCCATTAATTTAAAATCATAATTCCCCTTTGATTAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTTAAGTATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTc                                                                                                                                   <  6:1321358/146‑1 (MQ=255)
                         tAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGa                                                                                                                                >  7:327313/1‑150 (MQ=255)
                          aaCAAATGGGGGCACCGGCAGGTGCCATGCATGTAACATCATAATTCCACTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCCCTCATCATCTGAAGGATATTTGGGGGAGGAGGAGCGGGGCGGATAACTTTCGGact                                                                                                                              >  6:1259207/1‑149 (MQ=255)
                            cAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                              >  1:1310030/1‑149 (MQ=255)
                            aaatggggggCCCCGGCCGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCTCCTTGCCATAGCCTAATGCCGGATCACTCATCATCTGCAGGATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAg                                                                                                                            <  8:166008/146‑1 (MQ=255)
                             aaaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGc                                                                                                                           >  3:912218/1‑151 (MQ=255)
                             aaaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGc                                                                                                                           >  7:1351717/1‑151 (MQ=255)
                               aggggcgCACCGGCAGGTGCCATGAATAAAACATAATAAATCGCCTTTGATTAAAATCTTATCACCTTGCCATTGCCTAATCCACGATCACCCATCATCTCAAGCATATTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCg                                                                                                                         <  2:1887564/149‑1 (MQ=255)
                               aTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCg                                                                                                                         >  6:1061351/1‑151 (MQ=255)
                               aTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCACTGTGATGAAAATCTGATCACCTTGACATAGAATAATGAAGGATCACTAATAATCTGAATGATATTTTGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCg                                                                                                                         >  6:667251/1‑151 (MQ=255)
                                    ggcccgggCATGTGCCATTCATTTAACATCATACTGCCGCTGTGATCAACATATGATCACCTTGCCATCGCCTATTGCCGGATCCCTCATAATCTCCTGGTTATGTGGGGGTGGCGGACGGCGGCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                    <  1:83929/145‑1 (MQ=255)
                                      cACCGGCTCGTGCCATGCATTAAACATCATAAATCCCCAGTTATCAAAATCTGACCACCTAGCCAAAGCCTACTCCACCATCACTCATCATCTGAAGGATTTTTGGGCGCGGCCGCCGCCGCCGGATAACTTTCGGAATAGCCGGATCAgg                                                                                                                  <  6:952856/151‑1 (MQ=255)
                                            cAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcc                                                                                                            >  8:1859933/1‑151 (MQ=255)
                                            cAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcc                                                                                                            >  1:340963/1‑151 (MQ=255)
                                             aGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcct                                                                                                           >  7:1691550/1‑151 (MQ=255)
                                             aGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcct                                                                                                           >  4:451233/1‑151 (MQ=255)
                                                 gCCATGCATTTAACATAATAATCCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATTTTTGGGGGCGGAGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTcctcct                                                                                                        <  2:150745/150‑1 (MQ=255)
                                                   cATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGa                                                                                                      >  6:1750536/1‑150 (MQ=255)
                                                      gCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCg                                                                                                  >  3:1172574/1‑151 (MQ=255)
                                                      gCATTTAACATCAAACTTCCCCTGTGATGAAAATCTCATCACCTTCCCATACCCTAATCCAGGATCACTCATCATCTGAAGTATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCg                                                                                                  <  2:862855/151‑1 (MQ=255)
                                                      ccATTTAACATCATAATTCCACTCTCATCAAAATCTCATCACCTTCCCATATCCTAATCCACCATCACTCATCTTCTGTATCATACTGGGGGCCCGCCCACGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAcc                                                                                                   <  4:288888/149‑1 (MQ=255)
                                                       cATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCgg                                                                                                 >  4:1890011/1‑151 (MQ=255)
                                                          aaaaCATCATAATACCCCTGTCATCAAAATCCGACCACCTCCCCATCGCCTAATGCAGCATCACTCATCATCTGACGTATATTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCtt                                                                                              <  1:1590303/149‑1 (MQ=255)
                                                           tAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTACCTCCTGAACCGGCta                                                                                              >  6:1636806/1‑149 (MQ=255)
                                                           tAACATCATAATTCCCCTCTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTCAAGCATATTTGGGGGACGAGCACGGGGGCGGATAACTTTCGGAATACCCCGATCAGGAATTCCTCATGAACCGGCtt                                                                                              >  6:587605/1‑150 (MQ=255)
                                                            aacaacATAATCCCCCTGTGAACAAACGCTGACCACCTCCCCAAACCCTAATCCAGCAGCACTCATCATCTGAAGGATATGGGGGCGAGGAGGCCGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                            <  5:798493/151‑1 (MQ=255)
                                                            aaCATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                            >  7:1457240/1‑151 (MQ=255)
                                                            aaCATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                            >  5:1232770/1‑151 (MQ=255)
                                                            aaCATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCACGATTTCCTCCTGAACCGGCTTAc                                                                                            >  7:971015/1‑151 (MQ=255)
                                                                   tAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                     >  1:1430432/1‑151 (MQ=255)
                                                                    aaTTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                     >  2:1042993/1‑150 (MQ=255)
                                                                     aTTCCCCTGTGATTAAAATCTTCTCACCTTGCCATATCCTAATGCAGTATCACTCATAATCTGTTGGATTTTGGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCaa                                                                                   <  2:1654115/151‑1 (MQ=255)
                                                                        ccccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATGACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg                                                                                 >  1:1064226/1‑150 (MQ=255)
                                                                        ccccTGAGATTAAAATCGGATCACCTTTCCATAGCCTAATGCAGCATCACTCATCATCTGAAGGATATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg                                                                                 <  7:1577431/150‑1 (MQ=255)
                                                                             gtgATTAAAATCTCATCACCTTCCCAAAGCCTAATCCAGGATCACTCATCATCTGAAGGATATTGGGGGGAGGAGGACGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTa                                                                             <  1:1543262/149‑1 (MQ=255)
                                                                                atgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGt                                                                        >  7:119772/1‑151 (MQ=255)
                                                                                   aaaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtttt                                                                     >  5:1381736/1‑151 (MQ=255)
                                                                                    aaaTCTGACCACCTTGCCATACCCTAATGCAGCATCACTCATCATCTGACGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                    <  3:663248/151‑1 (MQ=255)
                                                                                      aTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                    >  5:1729360/1‑149 (MQ=255)
                                                                                       tCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagc                                                                 >  2:436991/1‑151 (MQ=255)
                                                                                        cTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                >  6:1907840/1‑151 (MQ=255)
                                                                                         tGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                >  3:1378227/1‑150 (MQ=255)
                                                                                         tGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                >  2:1709524/1‑150 (MQ=255)
                                                                                          gaccacctccccaacgCCTAAGCCAGGAGCACTCAACATCTGAAGGATTTGGGGGGGCGCCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccag                                                              <  5:1670217/136‑1 (MQ=255)
                                                                                          gATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                >  1:1848041/1‑149 (MQ=255)
                                                                                            tCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCt                                                            >  1:1012693/1‑151 (MQ=255)
                                                                                            gccccTTGCCATCGCCTAATGCAGGATCACTCATCATCTGAAGGTTATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCt                                                            <  6:350529/148‑1 (MQ=255)
                                                                                                cTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg                                                        >  8:76474/1‑151 (MQ=255)
                                                                                                cTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg                                                        >  7:447256/1‑151 (MQ=255)
                                                                                                  tcccATCGCCTAACGCCGGATCACTCATCCTCTCACGGATTTTTGGGGGCGGAGCCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg                                                        <  4:1200060/147‑1 (MQ=255)
                                                                                                  tcccATAGCCTAAGCCAGGACCACTCATCATCTGAAGGATATTTGGGGGCGGAGCCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGa                                                       <  4:771598/148‑1 (MQ=255)
                                                                                                  tGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATATGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAATTCCTCCTGAACCCGCTTACAGAACGCAAGGAATACTGGTTTTCAGCCAGCTGACGa                                                       >  8:1256715/1‑150 (MQ=255)
                                                                                                  acccATACCCTAATGCCGCATCACTCAACATCTGAATGATATTTGGGGGAGCCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGAt                                                      <  5:46436/149‑1 (MQ=255)
                                                                                                   gCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATc                                                     >  3:1306420/1‑151 (MQ=255)
                                                                                                    ccATAGCCTAATCCCGGATCACTCATCATCTGAAGGATTTTGGGGGGAGGAGGCGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCg                                                    <  4:1125196/151‑1 (MQ=255)
                                                                                                      aTAGCCAAATGCAGGACCACTCATCATCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTg                                                  <  7:776156/151‑1 (MQ=255)
                                                                                                       tAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTgg                                                 >  4:638763/1‑151 (MQ=255)
                                                                                                       accccTAATGCCGGATCACTCATGATCTTAAGGATATGTGGGGGACGCGGCCGCGCGCGGATAACTTTCGGAATAGCCGGAGCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTgg                                                 <  6:1153649/148‑1 (MQ=255)
                                                                                                        agacTAATGCAGGATCACTCATCATCTGAATGATTTTTGGGGGAGGAGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTgg                                                 <  8:454144/147‑1 (MQ=255)
                                                                                                        aGCCTAATGCAGGATCACTCATCATCTGACGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggc                                                <  1:220729/151‑1 (MQ=255)
                                                                                                         gCCTAATGCAGGATCACTCATCATCTGATGTTTATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcg                                               <  8:266061/151‑1 (MQ=255)
                                                                                                         cccTAATCCACCACCACTCATCAGCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcg                                               <  6:437358/150‑1 (MQ=255)
                                                                                                            aacagcagcaTCACTCATCATCTGAAGTATATTTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGgcggcg                                            <  7:561834/147‑1 (MQ=255)
                                                                                                              aTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGtt                                          >  4:1572736/1‑151 (MQ=255)
                                                                                                                ggaGGACCACTCATCATCTGAACGATTTTTGGGGGCGCTGCCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACTCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTa                                        <  8:855334/149‑1 (MQ=255)
                                                                                                                   gcagcccTCATCATGTGATGGATATGTGGGGGAGGAGGCCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAAc                                     <  3:485737/145‑1 (MQ=255)
                                                                                                                        aCTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc                                 >  5:186799/1‑150 (MQ=255)
                                                                                                                           catcatCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                             >  1:657589/1‑151 (MQ=255)
                                                                                                                           catcatCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                             >  1:484617/1‑151 (MQ=255)
                                                                                                                             tcatcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCa                            >  4:1472606/1‑150 (MQ=255)
                                                                                                                              catcAGAAGGATATTGGGGGGCGGCCGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAAt                          <  1:121342/151‑1 (MQ=255)
                                                                                                                              caccTGACGGATAGTTGGCGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAAt                          <  5:1062717/148‑1 (MQ=255)
                                                                                                                                  tGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCTCCGTTTAAACATCCAGCCAATCAAc                      >  6:1556374/1‑151 (MQ=255)
                                                                                                                                  tGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                      >  7:1084360/1‑151 (MQ=255)
                                                                                                                                   gatggATATTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                     <  6:1414258/148‑1 (MQ=255)
                                                                                                                                   gacgtatagTTGGGGGCGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                     <  2:1293263/146‑1 (MQ=255)
                                                                                                                                      ggATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGca                  <  2:174442/151‑1 (MQ=255)
                                                                                                                                            ttGGGGGAGTTGGCGGTGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca             <  6:846465/150‑1 (MQ=255)
                                                                                                                                            ttGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca             <  7:1765175/150‑1 (MQ=255)
                                                                                                                                            ttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca             >  3:1044493/1‑150 (MQ=255)
                                                                                                                                                  gcggaggaCGGGGGCGGATAACTTTAGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCGAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcg      <  8:1358265/149‑1 (MQ=255)
                                                                                                                                                  gaggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcg      >  4:1771052/1‑151 (MQ=255)
                                                                                                                                                  gagcaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcg      <  3:207638/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
ATGAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAA  >  DH1/3448373‑3448673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑