Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,448,5260AC29.5% 732.7 / 119.4 414intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand):  ref base A (222/67);  new base C (0/121);  total (223/191)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 447 total aligned reads displayed.

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676
                                                                                                                                                      |                                                                                                                                                      
   aGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGTGGAGGAGGACgg                                                                                                                                                     >  8:573376/1‑151 (MQ=255)
    gTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggg                                                                                                                                                    <  1:588979/151‑1 (MQ=255)
     ttGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgggg                                                                                                                                                   >  5:1168588/1‑151 (MQ=255)
      tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTTCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgggg                                                                                                                                                   <  8:1702432/150‑1 (MQ=255)
      tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggggg                                                                                                                                                  >  5:438041/1‑151 (MQ=255)
      tGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGCACgcggg                                                                                                                                                  >  3:783421/1‑151 (MQ=255)
         tCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCgg                                                                                                                                               >  4:1513012/1‑151 (MQ=255)
          ctttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCgg                                                                                                                                               >  3:1381386/1‑150 (MQ=255)
              gctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAAc                                                                                                                                          >  1:366648/1‑151 (MQ=255)
                   ttttAACAAATGGGGGCTCCGGCAGGTGCCTTGAAATTAACATCATAATTCCCCTGTTATGAAAATCTCATCACCTTGCCATTGCCTAATGCAGGATCTCTCATCATCTGAAGTATATTTGGGGGCGGAGGACGGGGGCGGATAACTTTc                                                                                                                                      <  4:1794233/149‑1 (MQ=255)
                   tttaaaCAAATGGGGGCACCGGCAGGTGCCATTAATTTAAAATCATAATTCCCCTTTGATTAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTTAAGTATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTc                                                                                                                                      <  6:1321358/146‑1 (MQ=255)
                      tAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTTATCAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATTTTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGa                                                                                                                                   <  1:1849340/150‑1 (MQ=255)
                      tAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGa                                                                                                                                   >  7:327313/1‑150 (MQ=255)
                       aaCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                                 >  4:281892/1‑151 (MQ=255)
                         cAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                                 >  1:1310030/1‑149 (MQ=255)
                          aaaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGc                                                                                                                              >  3:912218/1‑151 (MQ=255)
                           aaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCCTCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGcc                                                                                                                             >  1:713408/1‑151 (MQ=255)
                            aggggcgCACCGGCAGGTGCCATGAATAAAACATAATAAATCGCCTTTGATTAAAATCTTATCACCTTGCCATTGCCTAATCCACGATCACCCATCATCTCAAGCATATTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCg                                                                                                                            <  2:1887564/149‑1 (MQ=255)
                             tGGGGGCACCGGCCGGTTCCATTCATTAAAAATAATAATTCCCCTGTCATGAAAATCTGATCACCTTGCCATACCCTAATGCAGGATCACTCATCATCTGAAGGTTATTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCg                                                                                                                            <  7:829463/150‑1 (MQ=255)
                              gggcgcccgggCAGGTGACATTCATTTAAAATAATAATTCCCCTGTTATCAAAATCTGATCACCTTGCAATAGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGCGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCgg                                                                                                                           <  2:1714799/141‑1 (MQ=255)
                                    cccGGCAGGTGCCATTCATTTAAAATCATAATTCCCCTTTGATCAAAATCTGATCACCTTGCCATACACTAATGCAGCATCACTCATCATGTGAAGGATTTTTGGGGGCGGAGGACGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGa                                                                                                                    <  5:1953483/150‑1 (MQ=255)
                                     ccGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAt                                                                                                                   >  1:637500/1‑151 (MQ=255)
                                           ggTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcctc                                                                                                             >  6:1618924/1‑151 (MQ=255)
                                              gCCATGCATTTAACATAATAATCCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATTTTTGGGGGCGGAGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTcctcct                                                                                                           <  2:150745/150‑1 (MQ=255)
                                                 atccaattAACATCCAAATCCCCCTGTGATGAAACCCTGCTCACCTTGCCCTCCCCTACTGCAGGATCACGCATCATCTGAAGGATATGGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAc                                                                                                       <  6:1180982/145‑1 (MQ=255)
                                                 aTGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAc                                                                                                       >  8:1268679/1‑151 (MQ=255)
                                                     attaatcatcatACTTCCCCTTTGTTGAAACTCTGATCACCTCGCCATACCCTCCTCCCGCCCCCCTCCTCATCTGATGGATATGTGGCGGCGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                                   <  2:1330106/145‑1 (MQ=255)
                                                     aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc                                                                                                   >  5:1808407/1‑151 (MQ=255)
                                                      tttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAGTTCCTCCTGAACCGGCt                                                                                                  >  7:1702874/1‑151 (MQ=255)
                                                       aaaaCATCATAATACCCCTGTCATCAAAATCCGACCACCTCCCCATCGCCTAATGCAGCATCACTCATCATCTGACGTATATTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCtt                                                                                                 <  1:1590303/149‑1 (MQ=255)
                                                        tAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCtt                                                                                                 >  1:851634/1‑150 (MQ=255)
                                                        aaacaacAGAAGTCCCCTGGGACCAAACTCGCAGCACCTTCCCATCCCCTCAACCAGCCTCACTCATCAGCTGACGGATATTGGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTa                                                                                                <  7:450266/150‑1 (MQ=255)
                                                         aaCATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCACGATTTCCTCCTGAACCGGCTTAc                                                                                               >  7:971015/1‑151 (MQ=255)
                                                               atatagccccAGTGATGAAACTCAGATCTCCATGCCTTACCCTAATGCCCGCGCCCTCATCATCAGAGCGATATTGGGGGGGGGCGCCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACg                                                                                         <  5:1295112/145‑1 (MQ=255)
                                                               atAATGCCCCTTTGAACAAAATCTGATCCCCTTGCCATCGCCTAATGCCCCATCACTCATCATCTGAAGGATTTTTGGGGGCGGCGGCCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACg                                                                                         <  6:541834/151‑1 (MQ=255)
                                                                tAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                        >  1:1430432/1‑151 (MQ=255)
                                                                 aaTTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGCATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                        >  2:672315/1‑150 (MQ=255)
                                                                  aTTCCCCTGTGATTAAAATCTTCTCACCTTGCCATATCCTAATGCAGTATCACTCATAATCTGTTGGATTTTGGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCaa                                                                                      <  2:1654115/151‑1 (MQ=255)
                                                                  aTTCCCCTGTGATCAAAATCTGATCACCTTGCCATCGCCTAATGCAGGATCACTCATCATCTCAAGTTTATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCaa                                                                                      <  4:1132107/151‑1 (MQ=255)
                                                                  aTTCCCCTCTCAAGAAACTCTCCTCACCCCGCCATCCCCTAATGCAGCATCACTCATCATCTGAAGGATATTTGGGGGCGGCGCCCGCGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCaa                                                                                      <  6:608277/151‑1 (MQ=255)
                                                                   ttCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCa                                                                                       >  1:1666165/1‑149 (MQ=255)
                                                                    tCCCCTGTCATTAAAATCTGATCACCTTGCCAAACCCTAATCCAGCATCCCTCATCATCTTAAGGATATGTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAg                                                                                     <  8:1469850/150‑1 (MQ=255)
                                                                     ccccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATGACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg                                                                                    >  1:1064226/1‑150 (MQ=255)
                                                                          gtgATTAAAATCTCATCACCTTCCCAAAGCCTAATCCAGGATCACTCATCATCTGAAGGATATTGGGGGGAGGAGGACGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTa                                                                                <  1:1543262/149‑1 (MQ=255)
                                                                            tataaaaatgttttaaCCTTGCGATAGCCTACTGCAGGATCACTCATCATCTGTAGTATTTTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTg                                                                             <  1:246313/135‑1 (MQ=255)
                                                                             atgaAAATCTCCTCCCCTTGCCAGACCCTAATCCAGCATCACTCCTCATCGGACGGCTATGGGGGGGCGGCGCCCGCGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGt                                                                           <  7:1369632/151‑1 (MQ=255)
                                                                              tgaAAATCTGATCACCTTGCCATAGCCTAATGCAGCATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtt                                                                          >  6:1122327/1‑151 (MQ=255)
                                                                               aaaaaTCTGACCACCTTGCCATCGCCTAATGCAGCATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGttt                                                                         <  5:162953/150‑1 (MQ=255)
                                                                                aaaaTCTGATCCCCTTCCCATACCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtttt                                                                        <  6:853191/151‑1 (MQ=255)
                                                                                aaaaTCTGATCACCTTGCCATAGCCTAATGCAGCATCACTCATCATCTGAAGGTTTTTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtttt                                                                        <  4:1345718/151‑1 (MQ=255)
                                                                                  aaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc                                                                       >  5:566834/1‑150 (MQ=255)
                                                                                    tCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagc                                                                    >  2:436991/1‑151 (MQ=255)
                                                                                     cTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                   >  6:1907840/1‑151 (MQ=255)
                                                                                      tGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                   >  6:1725738/1‑150 (MQ=255)
                                                                                         tCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCt                                                               >  1:1012693/1‑151 (MQ=255)
                                                                                         gccccTTGCCATCGCCTAATGCAGGATCACTCATCATCTGAAGGTTATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCt                                                               <  6:350529/148‑1 (MQ=255)
                                                                                          cACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTg                                                              >  7:1161211/1‑151 (MQ=255)
                                                                                            ccTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGAc                                                            >  2:1078626/1‑151 (MQ=255)
                                                                                             cTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg                                                           >  8:76474/1‑151 (MQ=255)
                                                                                                     agacTAATGCAGGATCACTCATCATCTGAATGATTTTTGGGGGAGGAGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTgg                                                    <  8:454144/147‑1 (MQ=255)
                                                                                                        cTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                                >  2:529450/1‑151 (MQ=255)
                                                                                                         aacagcagcaTCACTCATCATCTGAAGTATATTTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGgcggcg                                               <  7:561834/147‑1 (MQ=255)
                                                                                                          caggCAGGATCACTCATCATCTTCAGTATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                                <  2:594372/146‑1 (MQ=255)
                                                                                                           atccacgagcccccatcatCAGACGCATCTTGGGGCGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGtt                                             <  7:1815206/138‑1 (MQ=255)
                                                                                                            gccAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGttt                                            <  3:1425044/149‑1 (MQ=255)
                                                                                                            gccAGCATCACTCAGCCGCTGAAGGATATTTGGGGGAGCCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGttt                                            <  3:335483/149‑1 (MQ=255)
                                                                                                             ggaGGACCACTCATCATCTGAACGATTTTTGGGGGCGCTGCCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACTCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTa                                           <  8:855334/149‑1 (MQ=255)
                                                                                                             gCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCTGCCAGCTGACGATCGTGGCGGCGttt                                            >  3:172469/1‑150 (MQ=255)
                                                                                                               cggATCACTCATCATCTCAAGGATTTTTGGGGGCCGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaaa                                         <  4:504155/150‑1 (MQ=255)
                                                                                                               cccaTCACTCATAATCTGACTGATATTTGGGGGAGCCGCCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaaa                                         <  5:788755/148‑1 (MQ=255)
                                                                                                                ggATCACTCATCATCTGTAGGATCGTTGCGGGCGGCGGCCGCGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAAc                                        <  6:1539261/151‑1 (MQ=255)
                                                                                                                cgATCACTCATCATCTGAAGGATATTTGGGGGCCGCGGCCGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAAc                                        <  5:884830/150‑1 (MQ=255)
                                                                                                                   tCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGTTCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCATGAACCGGCTTACAGAACGAAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGTAGGCGTTTAAACATc                                     >  6:80378/1‑151 (MQ=255)
                                                                                                                     aCTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc                                    >  7:1605160/1‑150 (MQ=255)
                                                                                                                     aCTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc                                    >  7:123119/1‑150 (MQ=255)
                                                                                                                       tcatcaTCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGc                                 >  4:1075906/1‑151 (MQ=255)
                                                                                                                       aaatcataTGAAGGATATTTGGGGCAGCACCCCGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGc                                 <  3:555559/149‑1 (MQ=255)
                                                                                                                        catcatCTGCAGGATATTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAg                                  <  8:1410833/149‑1 (MQ=255)
                                                                                                                         atcatcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc                                >  3:673747/1‑150 (MQ=255)
                                                                                                                           catcAGAAGGATATTGGGGGGCGGCCGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAAt                             <  1:121342/151‑1 (MQ=255)
                                                                                                                            atcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                            >  2:295094/1‑151 (MQ=255)
                                                                                                                             tcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                            >  1:49900/1‑150 (MQ=255)
                                                                                                                              cTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCa                           >  3:1146776/1‑150 (MQ=255)
                                                                                                                                gatggATATTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACa                        <  6:1414258/148‑1 (MQ=255)
                                                                                                                                gacggATTTTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCa                           <  4:1679033/145‑1 (MQ=255)
                                                                                                                                gAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc                         >  8:1864554/1‑150 (MQ=255)
                                                                                                                                   ggATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGca                     <  2:174442/151‑1 (MQ=255)
                                                                                                                                    gATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                    <  5:1728630/151‑1 (MQ=255)
                                                                                                                                    gATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcac                    >  4:910064/1‑151 (MQ=255)
                                                                                                                                        tttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaac                 >  4:381295/1‑150 (MQ=255)
                                                                                                                                         ttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGTATTACTGGTTTTCAGCCAGCTGAGGATAGTGGCGGCGTTTAACCTTCCAGACAATCAACAGAacaaca                >  4:703335/1‑150 (MQ=255)
                                                                                                                                         ttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGAGGATCGTGGCGGCGTTTAAACATCCAGCGAATCAACAGCacaaca                >  2:939963/1‑150 (MQ=255)
                                                                                                                                                 ggaggcCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcgcg       <  1:173244/151‑1 (MQ=255)
                                                                                                                                                 ggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcgcg       <  2:116511/151‑1 (MQ=255)
                                                                                                                                                  gaggcCGGGGGCGGATAACTTTCAGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGa      <  6:969321/151‑1 (MQ=255)
                                                                                                                                                    ggaCGGGGGCGGATAACTTTCGGAATAGACGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGaa     >  3:147469/1‑150 (MQ=255)
                                                                                                                                                     gaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCCACAGCACAACAATCCACGCGAAAc   >  7:1516602/1‑151 (MQ=255)
                                                                                                                                                     gaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAAc   >  8:296440/1‑151 (MQ=255)
                                                                                                                                                      aCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAAcc  >  3:1318851/1‑151 (MQ=255)
                                                                                                                                                      aCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAAAGCACAACAATCCACGAGAAAcc  >  3:1270072/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAACC  >  DH1/3448376‑3448676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑