Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,906,8320CA31.7% 180.4 / 18.8 117intergenic (+3/‑32)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (49/22);  new base A (31/2);  total (92/25)
Fisher's exact test for biased strand distribution p-value = 5.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

GGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTT  >  DH1/3906690‑3906982
                                                                                                                                              |                                                                                                                                                       
ccgcactagcccgccccTCGGACCTATAACCTCTTTTATATAGCTCGCCGTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGat                                                                                                                                                 <  5:498065/142‑1 (MQ=14)
  ggcaacgacccccccTCGCTCGTAAAACGACGTTTATAGTCCTGTCTCTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGatat                                                                                                                                               <  7:934872/143‑1 (MQ=17)
   gcaacgccgcccccTCGCACCAACAACCACTTTTATATTCCTGGTTGTCTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATc                                                                                                                                              <  7:1057425/142‑1 (MQ=17)
         ccacccccTCGCTCCAACAAAGACTTTGATATTTCTCTCTCTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCaaaaaa                                                                                                                                        <  8:1711426/148‑1 (MQ=18)
            cccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCGATATCAAAAAAAGt                                                                                                                                     >  2:1399605/1‑151 (MQ=255)
            cccccTCGCACCAAAAACCACGTTGATATTGCTAGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTACAACCAAAAAAGGGGatgtactatatggt                                                                                                                                     >  8:1178880/1‑139 (MQ=18)
             ccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGTATAGATCAAAAAAAGtt                                                                                                                                    >  1:149749/1‑150 (MQ=18)
             ccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCACCCCCCTCGCACCAATGGAGGGGagttcctatattgtt                                                                                                                                    >  3:129769/1‑137 (MQ=18)
             ccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGAAGTGTATATAAAAAAAAGtt                                                                                                                                    >  1:140935/1‑150 (MQ=18)
              cccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATCgcggcgctggccacgactgta                                                                                                                                    >  5:1296290/1‑135 (MQ=18)
               ccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCTAGATCAAAAAAAGttat                                                                                                                                  >  7:346347/1‑148 (MQ=255)
               ccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGAACCAAAAGAGTGTAAATCAATTAAAGTat                                                                                                                                   >  4:1524482/1‑149 (MQ=18)
                    cACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTACCCCCCCTCGCACCAAAAAACGCGCAATAAAAAAAAGTAAGAtta                                                                                                                              >  8:37544/1‑148 (MQ=18)
                      ggtatcaacACGTTTTTATTGCTCAGTGTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtg                                                                                                                           <  7:1696195/143‑1 (MQ=18)
                      ccAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAATATACGCTATATCCATAAATGTAACAtccgtt                                                                                                                           >  7:1754366/1‑146 (MQ=17)
                        aacaaagaCGTTTTTATTGCTCGCACTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgtg                                                                                                                         <  7:1819185/144‑1 (MQ=255)
                          aaaCCACGTTGATATTGGTGCCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCg                                                                                                                       <  7:389542/151‑1 (MQ=255)
                           aaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGAGTTCTTACGGACGTGGGGGTTAAAGTCCCCCCCCGCGCACCAAAGAAGGTTATATCATTAAAAGtttgattaatggg                                                                                                                         >  5:1032867/1‑136 (MQ=17)
                             ccACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAATAACTATAAATCAAAAAAAGTTAGATgcatgatattac                                                                                                                    >  2:1349964/1‑140 (MQ=17)
                               aCGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATAGGAGAAATCAAAAAAAGTATGATGAAtgtgtta                                                                                                                      >  2:1298177/1‑144 (MQ=18)
                                cGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTg                                                                                                                 <  1:1401185/151‑1 (MQ=255)
                                 gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCTATATCAAAAAAAGTAAGATGAGTGTGGGAAGGTgg                                                                                                                >  5:1527787/1‑151 (MQ=255)
                                   tttaagttctctCACTTGGCTAAGGGGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGc                                                                                                               <  5:921435/142‑1 (MQ=21)
                                     ttagttctccgaCTCGGCGAAGGTGGCGGAATTGGTAGACGATCTAGCTTCATGTGTTAGTGTTCTTACGGACGTGGGGGTGCAAGTCCCCCCCCTCGCACCAAACGAGCCGATATCAAAAAAAGTAATCTGACTGTGCGAAGGTGGCgg                                                                                                             <  8:454744/139‑1 (MQ=18)
                                     atatTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGGACCAAAGAAGGGGAGATCAATTATAGTATGGTGAGTGTGTTAAGGTGGCgg                                                                                                             >  1:390654/1‑150 (MQ=17)
                                     atatTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGGATATATCAAAAAATGTATGATGAAtgatttttgatgccgg                                                                                                             >  6:1714060/1‑136 (MQ=17)
                                      tatatctcgccgtcggCGAAGGGGGAGGAATTGGTAGACGCGCTAGCTTCAGGTTTTAGTGTTCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGa                                                                                                            <  7:732645/136‑1 (MQ=21)
                                      aatttttgtttgtttgCGAAGGTTGCGGAATTGGTAGACGCGCTAGCTTGAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGTCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGa                                                                                                            <  2:1845405/135‑1 (MQ=18)
                                      aatagctccccgtttgCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGaa                                                                                                           <  7:209495/136‑1 (MQ=255)
                                       atTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTCAAGTCCCCCCCCGCGCCCCAAAAGAGGGGATATCAATAAAAGTTTGGTGCGTGTTGGTATGTGGCGGAAt                                                                                                          >  1:590506/1‑151 (MQ=17)
                                         ttttttttgtttgCGAAGGTGTCGGAATTGGTATACGCGCTAGCTTCTGGTTTTATTGTTCTTTCGGATGTGTTGTTTAAAGTCACTCGAATCGCACCAATCGTTTCGATATCATTAAAAGTATTATTTCTGTGCGAAGGTGGCGGAAtt                                                                                                         <  8:642951/138‑1 (MQ=255)
                                             cccACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCAAGCTTCAGGTGTTAGTGGTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTa                                                                                                     <  7:54755/148‑1 (MQ=255)
                                             cccACTCGGGGAAGGGGGCGGTCTTGGTCGACGCGCTTGCTTCAGGTGTTCGGGTCCGTACGGACGTCTCGGTGCAAGCCCCACCACTCGCACGACCCGCTACGATATCTTAATAAGTAATCTGACTCTGCGAAGGTGGCGGAATTGGTAg                                                                                                    <  1:513972/149‑1 (MQ=9)
                                             cGCAGTGCGCGTATGTGTAGGAAGCGGTAGACGGCCTAGCGTCAGGGGGAAGTGGTCTTCCGGTCAGGGGGGTGCTCGTCCCCGCCCTCGCACTCCAAGCTACGATATCTTTAAAAGTAATCTGTCTTTGCGAAGGTGGCGGAATTGGTAg                                                                                                    <  7:1537493/151‑1 (MQ=255)
                                             cGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGACGGTAGATCAATTATAGTTAGATGCCTGTGTGTTGCTGCCGGAATTGATa                                                                                                     >  1:328468/1‑150 (MQ=14)
                                              gCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAATTAGTCGACTTCATAAAATGTTAGATGAGTGTTCGATGGTGGCGGATTAGGt                                                                                                      >  3:631637/1‑148 (MQ=14)
                                              gCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGATCCGATTAGAAAAACAGTAAGCTGAATTGGCGATCGTGCCGGAAGTTGTAgc                                                                                                   >  7:653794/1‑150 (MQ=14)
                                                tctatgCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtg                                                                                                                           <  5:536415/120‑1 (MQ=255)
                                                 cTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGGGAAATCAATAAAAGTAAGATACCTGATATATGCTGGCGGAATTGATataagg                                                                                                >  2:919448/1‑146 (MQ=14)
                                                    ggCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCCCCAAAATTCGGGCTTTCAAAAAAAGTTTGTTGCCTGTGGTTTGGTGGCGGTAttcgattcatgg                                                                                               >  1:577476/1‑139 (MQ=2)
                                                     gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAATAAAAGTTAGATGCCTGTGCGATGGTGGCGGAATTGGTAGActgg                                                                                               >  3:208916/1‑145 (MQ=18)
                                                       gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGTGTATATCAAAAAATGTTAGTTGACTGTTGTTAGGTGGCTGATTTgatttaatagatagc                                                                                          >  7:325441/1‑137 (MQ=14)
                                                           gtgtctgAATTGGTAGACGAGGTTGTTTCTTGTTTATTTGTTCTTATTGACGTTTTGGTTAAATTCCCTAGCCTCGCACTAATCGTTTGGAAATCTTAAAAAGTAAGATGTCTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCa                                                                                      <  4:635286/145‑1 (MQ=9)
                                                                 gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACACGCGGGGATCTCAAAAAAAGTATGATGACTGTGCGAAGGTGGCGGAATGGGTAGCCGCGCTAGCTTCATGAGtt                                                                                >  3:589311/1‑151 (MQ=21)
                                                                  aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCACGCCCCAAAAGAGGCGATATAAAAAAAATTAAGATGACTTTCGTAAAGTGCCGGAATTCCTAAACGCTATAGATTCACGTGTTa                                                                               >  5:486358/1‑150 (MQ=12)
                                                                   aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAACAACGGGATATAAAAAAAAGTTTGATGAATGTTGGTTGGTGGCGGAATTGGAATACGCGGTAGCTTCATGTGTTat                                                                              >  3:1881960/1‑150 (MQ=17)
                                                                      ggTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAAAAAAAGTTAGATGACTGTGGGTAGGTGGCGGAATTGGTAGACGAGGGAGCTTCAGGTGTTAgtgt                                                                           >  5:549353/1‑151 (MQ=21)
                                                                                   gCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATAGGGGATATCAAAAAAAGTAAGATGAGTGTGGTAAGGTGGCGGAATTCGAATACGTGCTATCATCATGAGTTAGTGTAAATAAGGACGTg                                                              >  1:816923/1‑151 (MQ=18)
                                                                                   gCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAAAAAAAGTTTGATGACTGTTGTAAGGTGGCGGAATTGGTATAAGAGTTATCATCAGGTTTTAGTGTAATTACGGCCGTg                                                              >  5:1858135/1‑151 (MQ=18)
                                                                                     ttCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGTAGGGGAGATCAAATAAAGTTAGATGCCTGAGGGAAGGTGGCGGAATTGGTAGCATAGGTATCATCAGGTTTTAGGGTAATTAAGGCCGTggg                                                            >  8:273223/1‑151 (MQ=12)
                                                                                     ttCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTAGATCAAAAAAAGTATGATGAATGTGGTTTGGTGGCGGAATTGTTTTAATGGGTATCTTCATGTTTTAGTGAAATTACGGAAGTggg                                                            >  1:1274390/1‑151 (MQ=12)
                                                                                     ttCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAACACGCGAAATAAAAAAAAATAAAAATACAGATAGAAGATGGCGGAATTGGTTTAAAGAATATCAACAGGTTTTAGTGTAATTACGGACGTggg                                                            >  5:748769/1‑151 (MQ=9)
                                                                                       cAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGGGATATCAAAAAAAGTTTGATGCCTGTGGGTTGGTGGCGGAATTGGTTGACGGGGTATCTTCAGGTTTTACTGTCCTTAAGGCCGgggggg                                                          >  7:807740/1‑151 (MQ=21)
                                                                                       cAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATGAAATATAGTAAGTTGAATGGGCGCAGGTGCCGGCAGTCGTACACGCGCAAGCTTCAGGTGTTAGTGTCCTTACGGACGTggggt                                                          >  7:131234/1‑150 (MQ=21)
                                                                                        aGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCGATATCAAAAAAAGTTAGATGACTGTGGGTACGTGGCGGAATTCGTATACGCGCAACCCTCAGGGGTGACTGTACTAACGCCCGTggggct                                                         >  5:650458/1‑149 (MQ=18)
                                                                                         ggTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAAAGACGGGATTTGAATAAAAGGTATTTGACTTTGGTTAGGTGGCGGAATTTGTAGAACCGCAACCGACACGTGTTAGTGTCCGTCACGAAGTggggggg                                                        >  5:1503678/1‑149 (MQ=9)
                                                                                          gtgtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTATCCGCGGGAGCTTCAGGTGTTAGTGTCCTTACGGCCGTGGGGGttg                                                       >  5:576607/1‑150 (MQ=37)
                                                                                             ttAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAACAAACGAAGAGAGTACAAAAAAAAAAAGATGAATTTGCGAAGGTTGATGAAATGGTAGACCCGATAAATTAAAAAGATATTGTGCTTGCGGACGTggggggtgggg                                                    >  2:1804493/1‑145 (MQ=12)
                                                                                              tAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAGATCAAAAAAAGTTAGATGAGTGTGGGTAGGTGGCGGAATTTGTATACGGGGGATCGTCAGGGTTTAGTGTACATAAGGCCGGGGGCGTTcgcct                                                   >  5:357245/1‑147 (MQ=16)
                                                                                               actgtTCTTACGTCCGTGGGGGTTCAACTCCACCCCCTCGCACCAAACGACCCCATATCAAAAAAAGTCCGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑c                                                 <  3:1602578/149‑1 (MQ=21)
                                                                                               aGTATTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATACGGGATATCAATAAAAGTAAGATGACTGTGGGAAGGTGCCGGAATTTCTATAAGAGATATCTTCAGGTTTTACTGTAATTAAGGACGTGGCGGTTGAAga                                                   >  7:1328507/1‑149 (MQ=16)
                                                                                                  gtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGTAAATCAAATAATGTTAGATGACTGTGATATGGTGGCGGATTTTGAAGACGAGGTATCTTAATGTTTTACTCTCCTAACCGCAGTCGGGGTTCAAGc‑ccgc                                              >  1:1323103/1‑149 (MQ=12)
                                                                                                           gACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGCCTGTGCGAAGGTGGCGGAATTGGTAGCCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGG‑CCCCACCCTCGcc                                     >  1:1636514/1‑150 (MQ=255)
                                                                                                           gACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGCCTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGT‑CCCCCCCCACTCAc                                    >  7:1259555/1‑151 (MQ=21)
                                                                                                              gTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGATATCATATAATGTAAGTTGACTGTGAGTAGGTGGCGGAATGGGTAGACGGGCGAGCTTCAGGTTTTAGTGTACTAACGCACGTGGGGCTTGTAGC‑cccctggctggccgc                                   >  3:125763/1‑139 (MQ=16)
                                                                                                              gTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCATTACGGAAGTGGGGGTTGAAGG‑aaccccccatgcccc                                   >  3:1897641/1‑143 (MQ=25)
                                                                                                                 ggggTTCACCGCCCCCCCCTCCCACCAACAGCTCCGACATCAAAAATAGTACGAGGACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAAtt                               <  7:37589/151‑1 (MQ=14)
                                                                                                                     ttCAAGTCCCCCCCCTCGCACCAAAGGAGGGGAGATCAAAAAATGTTTGATGCGTGTGCGTAGGTGGCGGAATTGGTAGACGGGCGAGCTTCAGGTGGTAGTGTCGTTGCGGCCGTGGGGCTTGAAGG‑CCCACCCCTCGCAGCAAGTAtgt                           >  8:1590051/1‑149 (MQ=16)
                                                                                                                           tCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTATACGCGCTAGCTTCAGGTGTTAGTGTAGTTACGGACGTGGGGGTTGAAGT‑CACCCCCCAGGCCCCaacttttttttatt                     >  5:1338362/1‑139 (MQ=21)
                                                                                                                           tCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTAGAAGAGCTAGCTTCAGGTGTTAGTGTCATTACGGACGTGGGGGTTGAAGT‑GCCCCCACTCGCACCCAGTAtggtt                         >  8:1838408/1‑143 (MQ=255)
                                                                                                                           tCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGGGGTAGCTTCCGGTGTTCGTGTCGTTCCGGCCGTGGGGGTTCGAGG‑CCCCCCCCGCGCACCAAtattgtgtgctt                     >  4:1794229/1‑140 (MQ=21)
                                                                                                                            ccccccccTCGCACCAAAAGAGGCGATTTCAAAAAAAGTAAGATGACTGTGCGATGGTGGCGGAATTCGTAGACGCGCTAGCTTCAGGTGTTAGTCTCGTTACGGACGTGCGGGTTGAAGG‑CCCCGGCCAAGCCCCAAATTTGTTTACtac                    >  5:1671449/1‑149 (MQ=18)
                                                                                                                             cccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTcc                   >  4:754672/1‑151 (MQ=255)
                                                                                                                              ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATGTTTACTTCCt                  >  8:1325097/1‑151 (MQ=255)
                                                                                                                               cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCtt                 >  3:1207357/1‑151 (MQ=255)
                                                                                                                                ccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTGAAGT‑CCCCCCCCTCGCACCAATTATCATTACTTCCtat                >  1:1797528/1‑149 (MQ=255)
                                                                                                                                 cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCgtt                >  6:338944/1‑150 (MQ=255)
                                                                                                                                 cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTc               >  1:7782/1‑151 (MQ=255)
                                                                                                                                  ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCt              >  3:734332/1‑151 (MQ=255)
                                                                                                                                  ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATGTTTAGTTCCTTTCt              >  6:361680/1‑151 (MQ=255)
                                                                                                                                  ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTACTTTCt              >  5:872062/1‑151 (MQ=255)
                                                                                                                                   cTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTAACTTCCTATCtt             >  2:1436209/1‑151 (MQ=255)
                                                                                                                                    tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAAATAACTTTACTTGCTTTCttt            >  7:1195884/1‑150 (MQ=255)
                                                                                                                                    tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCGCGCCCCAATTATCTTTACTTGCTTTCttt            >  7:62435/1‑150 (MQ=255)
                                                                                                                                     cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGt           >  6:826488/1‑151 (MQ=255)
                                                                                                                                     cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGt           >  1:1068197/1‑151 (MQ=255)
                                                                                                                                     cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGt           >  3:74936/1‑151 (MQ=255)
                                                                                                                                     cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTGtgttgt           >  6:538586/1‑151 (MQ=255)
                                                                                                                                      gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtt          >  1:217684/1‑151 (MQ=255)
                                                                                                                                      gCACCAAAAGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTATACGCGATAGCTTCAGGTGTTAGTGTACTTACTGACGTGGGGGTTAAAGT‑CCCCACAAACGCACCAAATATCGTAAATTACTTTATTGtt          >  6:75531/1‑151 (MQ=18)
                                                                                                                                       cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑GCCCCCCCGCGCACCAATTATCTTTACTTGCGTTGTTGttt         >  3:599328/1‑151 (MQ=255)
                                                                                                                                       cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGttt         >  5:980661/1‑151 (MQ=255)
                                                                                                                                        aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCCAGTGCCCCCCCCGCGCCCCAATTATCTTTACTTGCTTTCATGttt         >  5:445792/1‑151 (MQ=21)
                                                                                                                                         ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAACT‑GCCCCCCCTCGCACCAATTATCGTTACTTCCTTTCTTGTttct       >  3:488270/1‑151 (MQ=255)
                                                                                                                                           aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGATGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCGTTTCTTGTttcttc     >  8:552940/1‑151 (MQ=255)
                                                                                                                                            aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTGTTcc    >  3:248043/1‑151 (MQ=255)
                                                                                                                                            aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTcc    >  3:1431691/1‑151 (MQ=255)
                                                                                                                                            aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCGCGCCCCAATTATCTTTACTTCCTTTGTTGTTTGTTcc    >  7:1793634/1‑151 (MQ=255)
                                                                                                                                             aCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCGTTCTTGTTTCTTCCt   >  6:1809800/1‑151 (MQ=255)
                                                                                                                                              cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCtt  >  3:699201/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                       
GGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTT  >  DH1/3906690‑3906982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑