Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,906,8370CG25.4% 228.8 / 25.1 129intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (66/25);  new base G (30/1);  total (103/26)
Fisher's exact test for biased strand distribution p-value = 4.18e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

CCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906699‑3906987
                                                                                                                                          |                                                                                                                                                       
ccacccccTCGCTCCAACAAAGACTTTGATATTTCTCTCTCTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCaaaaaa                                                                                                                                             <  8:1711426/148‑1 (MQ=18)
   cccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCGATATCAAAAAAAGt                                                                                                                                          >  2:1399605/1‑151 (MQ=255)
   cccccTCGCACCAAAAACCACGTTGATATTGCTAGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTACAACCAAAAAAGGGGatgtactatatggt                                                                                                                                          >  8:1178880/1‑139 (MQ=18)
    ccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGTATAGATCAAAAAAAGtt                                                                                                                                         >  1:149749/1‑150 (MQ=18)
     cccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATCgcggcgctggccacgactgta                                                                                                                                         >  5:1296290/1‑135 (MQ=18)
      ccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCTAGATCAAAAAAAGttat                                                                                                                                       >  7:346347/1‑148 (MQ=255)
      ccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGAACCAAAAGAGTGTAAATCAATTAAAGTat                                                                                                                                        >  4:1524482/1‑149 (MQ=18)
          gCACCAAAACCCACGTTCATATTCCTCGCACCTCTAGAAGTAGCCGCATTTCTTAGTATCTCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGa                                                                                                                                   <  6:228246/151‑1 (MQ=18)
           cACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTACCCCCCCTCGCACCAAAAAACGCGCAATAAAAAAAAGTAAGAtta                                                                                                                                   >  8:37544/1‑148 (MQ=18)
             ggtatcaacACGTTTTTATTGCTCAGTGTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtg                                                                                                                                <  7:1696195/143‑1 (MQ=18)
               aacaaagaCGTTTTTATTGCTCGCACTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgtg                                                                                                                              <  7:1819185/144‑1 (MQ=255)
                 aaaCCACGTTGATATTGGTGCCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCg                                                                                                                            <  7:389542/151‑1 (MQ=255)
                    ccACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAATAACTATAAATCAAAAAAAGTTAGATgcatgatattac                                                                                                                         >  2:1349964/1‑140 (MQ=17)
                       cGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTg                                                                                                                      <  1:1401185/151‑1 (MQ=255)
                        gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCTATATCAAAAAAAGTAAGATGAGTGTGGGAAGGTgg                                                                                                                     >  5:1527787/1‑151 (MQ=255)
                          tttaagttctctCACTTGGCTAAGGGGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGc                                                                                                                    <  5:921435/142‑1 (MQ=21)
                            ttagttctccgaCTCGGCGAAGGTGGCGGAATTGGTAGACGATCTAGCTTCATGTGTTAGTGTTCTTACGGACGTGGGGGTGCAAGTCCCCCCCCTCGCACCAAACGAGCCGATATCAAAAAAAGTAATCTGACTGTGCGAAGGTGGCgg                                                                                                                  <  8:454744/139‑1 (MQ=18)
                            atatTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGGATATATCAAAAAATGTATGATGAAtgatttttgatgccgg                                                                                                                  >  6:1714060/1‑136 (MQ=17)
                             tatatctcgccgtcggCGAAGGGGGAGGAATTGGTAGACGCGCTAGCTTCAGGTTTTAGTGTTCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGa                                                                                                                 <  7:732645/136‑1 (MQ=21)
                             aatttttgtttgtttgCGAAGGTTGCGGAATTGGTAGACGCGCTAGCTTGAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGTCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGa                                                                                                                 <  2:1845405/135‑1 (MQ=18)
                             aatagctccccgtttgCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGaa                                                                                                                <  7:209495/136‑1 (MQ=255)
                                ttttttttgtttgCGAAGGTGTCGGAATTGGTATACGCGCTAGCTTCTGGTTTTATTGTTCTTTCGGATGTGTTGTTTAAAGTCACTCGAATCGCACCAATCGTTTCGATATCATTAAAAGTATTATTTCTGTGCGAAGGTGGCGGAAtt                                                                                                              <  8:642951/138‑1 (MQ=255)
                                 actctgtgtatgCGAAGGTTGCGGAATTGGTAGAGGTGCTAGCTTGAGGTGTTAGTTTAATTACTGACGTTGCGGTTCAAGTCCCTCCACTCGCACCAAATGATTCGATATCATTTAAAGTAATTTGAGTGTGCGAAGGTGGCGGAATTg                                                                                                             <  4:900018/139‑1 (MQ=255)
                                   tCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCCCCAAAAGAGGCGATATCCAAAAAACTGAGATGCCGGGGCGAACGGGGCgggcctgggg                                                                                                          >  7:185941/1‑143 (MQ=17)
                                    cccACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCAAGCTTCAGGTGTTAGTGGTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTa                                                                                                          <  7:54755/148‑1 (MQ=255)
                                    cccACTCGGGGAAGGGGGCGGTCTTGGTCGACGCGCTTGCTTCAGGTGTTCGGGTCCGTACGGACGTCTCGGTGCAAGCCCCACCACTCGCACGACCCGCTACGATATCTTAATAAGTAATCTGACTCTGCGAAGGTGGCGGAATTGGTAg                                                                                                         <  1:513972/149‑1 (MQ=9)
                                    cGCAGTGCGCGTATGTGTAGGAAGCGGTAGACGGCCTAGCGTCAGGGGGAAGTGGTCTTCCGGTCAGGGGGGTGCTCGTCCCCGCCCTCGCACTCCAAGCTACGATATCTTTAAAAGTAATCTGTCTTTGCGAAGGTGGCGGAATTGGTAg                                                                                                         <  7:1537493/151‑1 (MQ=255)
                                    cGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGACGGTAGATCAATTATAGTTAGATGCCTGTGTGTTGCTGCCGGAATTGATa                                                                                                          >  1:328468/1‑150 (MQ=14)
                                     gCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGATCCGATTAGAAAAACAGTAAGCTGAATTGGCGATCGTGCCGGAAGTTGTAgc                                                                                                        >  7:653794/1‑150 (MQ=14)
                                       tctatgCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtg                                                                                                                                <  5:536415/120‑1 (MQ=255)
                                        cTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGGGAAATCAATAAAAGTAAGATACCTGATATATGCTGGCGGAATTGATataagg                                                                                                     >  2:919448/1‑146 (MQ=14)
                                           ggCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCCCCAAAATTCGGGCTTTCAAAAAAAGTTTGTTGCCTGTGGTTTGGTGGCGGTAttcgattcatgg                                                                                                    >  1:577476/1‑139 (MQ=2)
                                            gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAATAAAAGTTAGATGCCTGTGCGATGGTGGCGGAATTGGTAGActgg                                                                                                    >  3:208916/1‑145 (MQ=18)
                                              gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGTGTATATCAAAAAATGTTAGTTGACTGTTGTTAGGTGGCTGATTTgatttaatagatagc                                                                                               >  7:325441/1‑137 (MQ=14)
                                                  gtgtctgAATTGGTAGACGAGGTTGTTTCTTGTTTATTTGTTCTTATTGACGTTTTGGTTAAATTCCCTAGCCTCGCACTAATCGTTTGGAAATCTTAAAAAGTAAGATGTCTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCa                                                                                           <  4:635286/145‑1 (MQ=9)
                                                        gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACACGCGGGGATCTCAAAAAAAGTATGATGACTGTGCGAAGGTGGCGGAATGGGTAGCCGCGCTAGCTTCATGAGtt                                                                                     >  3:589311/1‑151 (MQ=21)
                                                         aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCACGCCCCAAAAGAGGCGATATAAAAAAAATTAAGATGACTTTCGTAAAGTGCCGGAATTCCTAAACGCTATAGATTCACGTGTTa                                                                                    >  5:486358/1‑150 (MQ=12)
                                                          aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAACAACGGGATATAAAAAAAAGTTTGATGAATGTTGGTTGGTGGCGGAATTGGAATACGCGGTAGCTTCATGTGTTat                                                                                   >  3:1881960/1‑150 (MQ=17)
                                                             ggTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAAAAAAAGTTAGATGACTGTGGGTAGGTGGCGGAATTGGTAGACGAGGGAGCTTCAGGTGTTAgtgt                                                                                >  5:549353/1‑151 (MQ=21)
                                                                 gtcgcgCTAGCTTCTGGTTTTAGTCTTCTTACGGAAGTGGGGGTTCAAGTGCCCCCCCTCGCACGAAACGAGTCTATATCAAAAAAATTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCtt                                                                            <  7:61526/149‑1 (MQ=25)
                                                                     cgcgcgCTTCCGCTGTTAGTGTGCGTCCGGACGGGGGGGGGCCCGTCCCCCCCCGCGCACCAAACGATTCGATATCAAAAAAAGTAATAGGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACgg                                                                        <  1:475900/146‑1 (MQ=18)
                                                                      gcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTATATCAATAAAAGTTAGATGAATGTTAGTTGGTCGCGGAATTAGTATAAGAAATATCATCAGGTGTAAgtgaaattaagcc                                                                       >  6:892562/1‑141 (MQ=9)
                                                                        tAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAAGGAGGGGATATCAAATAAAGTTAGTTGCCTGTTGGTTGGTGGCGGGATTTCTATAAGTGATAGCATCATGTGTTACTGTCATTAAGGCCg                                                                     >  5:686743/1‑151 (MQ=12)
                                                                          gCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATAGGGGATATCAAAAAAAGTAAGATGAGTGTGGTAAGGTGGCGGAATTCGAATACGTGCTATCATCATGAGTTAGTGTAAATAAGGACGTg                                                                   >  1:816923/1‑151 (MQ=18)
                                                                            ttCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGTAGGGGAGATCAAATAAAGTTAGATGCCTGAGGGAAGGTGGCGGAATTGGTAGCATAGGTATCATCAGGTTTTAGGGTAATTAAGGCCGTggg                                                                 >  8:273223/1‑151 (MQ=12)
                                                                            ttCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTAGATCAAAAAAAGTATGATGAATGTGGTTTGGTGGCGGAATTGTTTTAATGGGTATCTTCATGTTTTAGTGAAATTACGGAAGTggg                                                                 >  1:1274390/1‑151 (MQ=12)
                                                                            ttCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAACACGCGAAATAAAAAAAAATAAAAATACAGATAGAAGATGGCGGAATTGGTTTAAAGAATATCAACAGGTTTTAGTGTAATTACGGACGTggg                                                                 >  5:748769/1‑151 (MQ=9)
                                                                              cAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGAGGGGATATCAAAAAAAGTTTGATGCCTGTGGGTTGGTGGCGGAATTGGTTGACGGGGTATCTTCAGGTTTTACTGTCCTTAAGGCCGgggggg                                                               >  7:807740/1‑151 (MQ=21)
                                                                              cAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATGAAATATAGTAAGTTGAATGGGCGCAGGTGCCGGCAGTCGTACACGCGCAAGCTTCAGGTGTTAGTGTCCTTACGGACGTggggt                                                               >  7:131234/1‑150 (MQ=21)
                                                                               aGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCGATATCAAAAAAAGTTAGATGACTGTGGGTACGTGGCGGAATTCGTATACGCGCAACCCTCAGGGGTGACTGTACTAACGCCCGTggggct                                                              >  5:650458/1‑149 (MQ=18)
                                                                                 gtgtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTATCCGCGGGAGCTTCAGGTGTTAGTGTCCTTACGGCCGTGGGGGttg                                                            >  5:576607/1‑150 (MQ=37)
                                                                                    ttAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAACAAACGAAGAGAGTACAAAAAAAAAAAGATGAATTTGCGAAGGTTGATGAAATGGTAGACCCGATAAATTAAAAAGATATTGTGCTTGCGGACGTggggggtgggg                                                         >  2:1804493/1‑145 (MQ=12)
                                                                                     tAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAGATCAAAAAAAGTTAGATGAGTGTGGGTAGGTGGCGGAATTTGTATACGGGGGATCGTCAGGGTTTAGTGTACATAAGGCCGGGGGCGTTcgcct                                                        >  5:357245/1‑147 (MQ=16)
                                                                                      actgtTCTTACGTCCGTGGGGGTTCAACTCCACCCCCTCGCACCAAACGACCCCATATCAAAAAAAGTCCGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑c                                                      <  3:1602578/149‑1 (MQ=21)
                                                                                       gtgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAAAGAGGGTAGTTCAAAAAAAGTTAGATGCCTGTTGGTTGGTGGCGGATTTGGGTTAAGGGGGATCTTCAGGGGTTAGTGTCCATAAGGCcgggcggggggtaggcc                                                      >  5:491970/1‑142 (MQ=9)
                                                                                         gtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGTAAATCAAATAATGTTAGATGACTGTGATATGGTGGCGGATTTTGAAGACGAGGTATCTTAATGTTTTACTCTCCTAACCGCAGTCGGGGTTCAAGc‑ccgc                                                   >  1:1323103/1‑149 (MQ=12)
                                                                                         gtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACCAGGTTAAATCCTAAAGAGTATGATGCATGTTTTATGGTGGCGGAATTCGATGAAGTGATATCTGCAGGTTGGAATGTCCATAAGGCCGTGGGGCTTCAAggcaac                                                    >  5:166644/1‑146 (MQ=9)
                                                                                                  gACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATGGACGCGATATCAAAAAATGTAAGATGAGTGTGGTAAGGTGGCGGAATTGGTATACGGGATAGCTTCAGGTGTTAGTGTACTATGGGACGGGGGGGTTGAAGtaacaaccaaagcc                                           >  3:1444866/1‑138 (MQ=19)
                                                                                                  gACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGCCTGTGCGAAGGTGGCGGAATTGGTAGCCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGG‑CCCCACCCTCGcc                                          >  1:1636514/1‑150 (MQ=255)
                                                                                                  gACGTGGGGGTTCAAGT‑CCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGCCTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGT‑CCCCCCCCACTCAc                                         >  7:1259555/1‑151 (MQ=21)
                                                                                                     gTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGATATCATATAATGTAAGTTGACTGTGAGTAGGTGGCGGAATGGGTAGACGGGCGAGCTTCAGGTTTTAGTGTACTAACGCACGTGGGGCTTGTAGC‑cccctggctggccgc                                        >  3:125763/1‑139 (MQ=16)
                                                                                                            ttCAAGTCCCCCCCCTCGCACCAAAGGAGGGGAGATCAAAAAATGTTTGATGCGTGTGCGTAGGTGGCGGAATTGGTAGACGGGCGAGCTTCAGGTGGTAGTGTCGTTGCGGCCGTGGGGCTTGAAGG‑CCCACCCCTCGCAGCAAGTAtgt                                >  8:1590051/1‑149 (MQ=16)
                                                                                                             tCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGT‑GCCGCCCCAGGCCCCAATTATAtt                               >  7:1403677/1‑151 (MQ=25)
                                                                                                                  tCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTATACGCGCTAGCTTCAGGTGTTAGTGTAGTTACGGACGTGGGGGTTGAAGT‑CACCCCCCAGGCCCCaacttttttttatt                          >  5:1338362/1‑139 (MQ=21)
                                                                                                                   ccccccccTCGCACCAAAAGAGGCGATTTCAAAAAAAGTAAGATGACTGTGCGATGGTGGCGGAATTCGTAGACGCGCTAGCTTCAGGTGTTAGTCTCGTTACGGACGTGCGGGTTGAAGG‑CCCCGGCCAAGCCCCAAATTTGTTTACtac                         >  5:1671449/1‑149 (MQ=18)
                                                                                                                    cccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTcc                        >  4:754672/1‑151 (MQ=255)
                                                                                                                     ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATGTTTACTTCCt                       >  8:1325097/1‑151 (MQ=255)
                                                                                                                      cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCtt                      >  3:1207357/1‑151 (MQ=255)
                                                                                                                       ccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCCAGT‑CCCCCCCCTCGGCCGCATTATCTTTACGTCCttt                     >  7:984690/1‑151 (MQ=21)
                                                                                                                       ccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTGAAGT‑CCCCCCCCTCGCACCAATTATCATTACTTCCtat                     >  1:1797528/1‑149 (MQ=255)
                                                                                                                        cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATGTTTACTTCCTTTc                    >  3:722674/1‑151 (MQ=255)
                                                                                                                        cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCgtt                     >  6:338944/1‑150 (MQ=255)
                                                                                                                        cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTc                    >  1:7782/1‑151 (MQ=255)
                                                                                                                         ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCt                   >  3:734332/1‑151 (MQ=255)
                                                                                                                         ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATGTTTAGTTCCTTTCt                   >  6:361680/1‑151 (MQ=255)
                                                                                                                           tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAAATAACTTTACTTGCTTTCttt                 >  7:1195884/1‑150 (MQ=255)
                                                                                                                           tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTGttt                 >  1:1629121/1‑150 (MQ=255)
                                                                                                                            cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGt                >  6:826488/1‑151 (MQ=255)
                                                                                                                            cGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGt                >  1:1068197/1‑151 (MQ=255)
                                                                                                                             gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtt               >  1:217684/1‑151 (MQ=255)
                                                                                                                             gCACCAAAAGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTATACGCGATAGCTTCAGGTGTTAGTGTACTTACTGACGTGGGGGTTAAAGT‑CCCCACAAACGCACCAAATATCGTAAATTACTTTATTGtt               >  6:75531/1‑151 (MQ=18)
                                                                                                                              cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑GCCCCCCCGCGCACCAATTATCTTTACTTGCGTTGTTGttt              >  3:599328/1‑151 (MQ=255)
                                                                                                                              cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGttt              >  5:980661/1‑151 (MQ=255)
                                                                                                                               aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCCAGTGCCCCCCCCGCGCCCCAATTATCTTTACTTGCTTTCATGttt              >  5:445792/1‑151 (MQ=21)
                                                                                                                                ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTAATTCCTTTCTTGTttct            >  3:304440/1‑151 (MQ=255)
                                                                                                                                ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAACT‑GCCCCCCCTCGCACCAATTATCGTTACTTCCTTTCTTGTttct            >  3:488270/1‑151 (MQ=255)
                                                                                                                                  aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGATGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCGTTTCTTGTttcttc          >  8:552940/1‑151 (MQ=255)
                                                                                                                                   aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTGTTcc         >  3:248043/1‑151 (MQ=255)
                                                                                                                                   aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTcc         >  3:1431691/1‑151 (MQ=255)
                                                                                                                                   aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTAGCCCCAATTATCTTTAATTCCTTTGTTGTTTCTTcc         >  8:195637/1‑151 (MQ=255)
                                                                                                                                   aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCGCGCCCCAATTATCTTTACTTCCTTTGTTGTTTGTTcc         >  7:1793634/1‑151 (MQ=255)
                                                                                                                                     cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGTTTCTTCCtt       >  1:988256/1‑151 (MQ=255)
                                                                                                                                     cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCtt       >  3:699201/1‑151 (MQ=255)
                                                                                                                                      gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGG‑CCCCCCCCTCGCACCAATTTTCTTTACTTCATTTGTTGTTTCGTCGTTg      >  4:1437450/1‑151 (MQ=21)
                                                                                                                                      gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGTTTCTTCCttt      >  2:1316678/1‑150 (MQ=255)
                                                                                                                                      gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTAAAGG‑CCCCCCCCTGGGACCAATTATATTTAAGTTCTTTGTTGTTTGTTCCttt      >  5:1371145/1‑150 (MQ=18)
                                                                                                                                       aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGCAGT‑CCCCCCCCGCGCCCCCAATTTTTTTTCTTGCTTTGTTGTTTGTTCCTTgt     >  7:372334/1‑150 (MQ=18)
                                                                                                                                       aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCCCCAATTATGTTTACTTCCTTTCTTGTTTcttgcttgt     >  2:543396/1‑150 (MQ=255)
                                                                                                                                       aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTACTTTCTTGTTTCTTCCTTg      >  4:1516934/1‑150 (MQ=255)
                                                                                                                                          cGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGAttt  >  8:1185559/1‑151 (MQ=255)
                                                                                                                                          |                                                                                                                                                       
CCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906699‑3906987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑