Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA30.4% 346.3 / 33.5 239intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (123/19);  new base A (61/1);  total (219/20)
Fisher's exact test for biased strand distribution p-value = 8.86e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

CAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613629‑613919
                                                                                                                                             |                                                                                                                                                       
     gaaagCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTACTATAGCAGAAGCACAAAGATTAATGAATGACAACGAAACTATAACAAAAAGAAAGAGGATTAACGATCATTATAAAAAGTTTTACGt                                                                                                                                            >  6:2093829/1‑150 (MQ=0)
       aagCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCGACGACACTATACCCAAAATACGCCGGCGTATCGGTACGTGTCACGTCTGTTACTTaa                                                                                                                                          >  1:151220/1‑150 (MQ=11)
          cGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAATATGAAGGACTGTCCACGCCACTAGACCCAAAAGAAAGCGGCTTATCGCTGAGTTTCACCTGTTTTACGTaaaaa                                                                                                                                       >  7:2152391/1‑150 (MQ=11)
            gCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGGTGCGGCGGGTTTGTGCTTTTTAGGGGGGAGGAACAGGAGTGACTGGGCAAGACACTATTGCCAAAAGACAGAGGCGTATCGGTCAGTTTAAAATGATTTACGTAATAAccg                                                                                                                                    >  5:2070437/1‑150 (MQ=2)
            gCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGATTAGCCTGGTTTTTGCTTTTGGAGGGTAAGAAAGATTAATTAATGTACAAGACACTATAAAAAAAAAAAATCGGATTATCGGTCAGTTTGACCTGTTGTACGTaaataggg                                                                                                                                    >  2:213140/1‑146 (MQ=2)
             cTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTTGAGTGGCAGATAGATGAATGCCTCTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGtaataaaca                                                                                                                                    >  7:1644347/1‑147 (MQ=11)
             cTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTTGAGGGGCAGAAAGATGAAAGACAGTCCACGACACTATACAAAAAAGAAAACCACTTAACGGTAAGTTAAAACTTATATACGTaaaaaaacc                                                                                                                                   >  6:993891/1‑147 (MQ=11)
                    gTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATTTACGTAAGAAcggcagaggcc                                                                                                                            >  3:1734121/1‑141 (MQ=11)
                      cAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACC‑Cgagatggg                                                                                                                           >  1:2155171/1‑142 (MQ=255)
                       aGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGACACTCTACCCAAAAGAAAGCGGCTTATCGCTCAGTTTCACCTGATGTACGTAAAAAAC‑CGCAA‑cggcgcg                                                                                                                       >  6:2278682/1‑149 (MQ=11)
                       aGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGTTC‑CGGCggg                                                                                                                       >  7:1375773/1‑151 (MQ=255)
                       aGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGATA‑CGGCggg                                                                                                                       >  2:2236488/1‑151 (MQ=255)
                        gTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGCTA‑CGGCGGGt                                                                                                                      >  3:1455684/1‑151 (MQ=25)
                          ttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGGTT‑CGGCGGGttt                                                                                                                    >  1:1917955/1‑151 (MQ=25)
                            cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGTTT‑CGGCGGGtttgt                                                                                                                  >  5:1434142/1‑149 (MQ=25)
                            cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CGGTA‑CGGCGGGtttgt                                                                                                                  >  6:1536913/1‑149 (MQ=255)
                            cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACC‑CGATA‑CGGCGGGtttat                                                                                                                  >  2:757571/1‑149 (MQ=255)
                            cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTGTCCCCGGGTGTACGTAAATCCC‑CGCTT‑CGGGGCGttttt                                                                                                                  >  3:747453/1‑151 (MQ=11)
                              ccTGGTTTACTTAAAACCCCGCTTCTGTTTTTTTTTGGTTTTGTAGGGGAAGAAATATGAATGAGTGTCCACGACACTATACCCAAAAGAAAGCTGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTAc                                                                                                                <  2:583651/151‑1 (MQ=11)
                               cTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTGTACGTAATAACC‑CGGTG‑CGGCGGGtttgtgct                                                                                                               >  5:652447/1‑146 (MQ=11)
                                tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTGTACGTAAAAACC‑CGGTG‑CGGCGGGTTTGTCCtt                                                                                                              >  1:424079/1‑151 (MQ=255)
                                  gTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTGTACGTAATAACC‑CGGTG‑CGGCGGGTTTGTCCtttt                                                                                                            >  1:2112429/1‑151 (MQ=11)
                                  gTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTG‑CGGCGGGTTTGTCCttgt                                                                                                            >  7:1294641/1‑149 (MQ=255)
                                   tttACGATAAAACCCGCCTCGGGTGGTTTTTTTTTTTTGTTGTAAATAAATATTAATGACGGACCACTACTCTATACACAAAAGAAAGCGGCTTATCGTTCAGTTTCAGCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACtttt                                                                                                            <  8:47851/150‑1 (MQ=2)
                                    ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGATT‑CGGCGGGTTTCTACtttttg                                                                                                          >  8:1874645/1‑149 (MQ=255)
                                    ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAACC‑CGGTG‑CGGCGGGTTTGTAAtttttg                                                                                                          >  4:114280/1‑149 (MQ=11)
                                     tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACCCTATACCCAAAAGAAAGCGGCTTAGCGCGGAGGAGCACCGGTGTGAAGTAAAGACC‑CGCTTGcggcggtcgtttggggtggt                                                                                                          >  7:1845263/1‑137 (MQ=2)
                                     tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACC‑CGGTG‑CGGCGGGTTTGTGCtttttg                                                                                                          >  3:1858707/1‑148 (MQ=11)
                                     accGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTTTATTTCCATAACGAGCAATCACTTTACACCACCCTATAACCAAAAGAAATCGGCTTACCCCTCAGTTTCACCTTTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGa                                                                                                         <  2:1552642/149‑1 (MQ=2)
                                      aCGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑CGATA‑CGGCGGGTTTTTTAtttttg                                                                                                          >  8:109554/1‑147 (MQ=11)
                                       cGTAAAAAGCCGGTTCGGGGGGTTTTTGCTTTTGGAGGCGCAGAAAGATGAATGACTGTCGACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAgg                                                                                                       <  5:999391/151‑1 (MQ=255)
                                       cGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTTGAgg                                                                                                       >  7:896237/1‑151 (MQ=25)
                                        gTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGGTT‑CGGCGGGTTTGAGCTTGTGGAggg                                                                                                      >  7:775716/1‑151 (MQ=255)
                                         tAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGTTTTACGTAATAACC‑CGGTT‑CGGCGGGTTTGTGCTTTTTGAgggg                                                                                                     >  3:1029389/1‑151 (MQ=11)
                                          aaaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAACC‑CGGTG‑CGGCGGGTTTGTTCTTGTTGAgtgcg                                                                                                    >  5:881237/1‑147 (MQ=11)
                                           aaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTCATTTTTGAGTGGcc                                                                                                   >  1:800435/1‑150 (MQ=255)
                                             aaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGATT‑CGGCGGGTTTCTGATTTTTGAGTGGCatt                                                                                                 >  6:1262839/1‑149 (MQ=11)
                                             aaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTGTACGTAAAAAAC‑CGGTT‑CGGCGGGTTTTTGCTTTTGGAGTGGc                                                                                                    >  1:624206/1‑148 (MQ=11)
                                             aaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑CGATA‑CGGCGGGTTTAAAATTTTTGAGTGGCAgt                                                                                                 >  4:1280701/1‑150 (MQ=11)
                                                ccGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACC‑CGATT‑CGGCGGGTTTAAAATTTTTGAgtgccatatat                                                                                              >  6:1928387/1‑141 (MQ=11)
                                                  gCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAAC‑CGATA‑CGGCGGGTTTAAAATTTTTGAGTGGCatatatt                                                                                             >  2:549686/1‑144 (MQ=11)
                                                   cTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACATGATTTACGTAAAAAAC‑CGGTG‑CGGCGGGTTTATAATTTTTGAgtgcgatatatac                                                                                            >  2:1985634/1‑138 (MQ=11)
                                                    ttCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAACCAAAAGAAAGCCCCTGATCGGACAGGTTCACCTCTTTTAAGTAAAAACA‑CGCTT‑CGGCGGGTTTTTACTATTGGAGGGGCAGAAAGatg                                                                                           >  7:1131762/1‑150 (MQ=11)
                                                       ggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTGAACTTTTTGAGGGGGAGaaataaaaaac                                                                                       >  8:1601119/1‑143 (MQ=11)
                                                       ggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTCTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑CGATA‑CGGCGGGTTTAAAATTTTGGAGTGCCATaaattaataac                                                                                       >  8:1995474/1‑143 (MQ=11)
                                                          ttgtttttgtttttGGTTGGTCATAAATATTATTGATTGTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGatgaatga                                                                                      <  8:1049145/147‑1 (MQ=11)
                                                              ttttGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACC‑CGGTG‑CGGCGGGTTTGTCATTTTTGAGGGGGATATAGatgagacccattcg                                                                                >  3:889239/1‑141 (MQ=11)
                                                                 tGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGTGGCAGAAATAAGATTGACttgcgacg                                                                             >  1:984330/1‑144 (MQ=11)
                                                                     tttGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAAC‑CGGTG‑CGGCGGGTTTGTGCTTGTGGAGGGGCAGAAAGatgtatctgtgtcggcgagac                                                                         >  7:1789392/1‑136 (MQ=11)
                                                                               cAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGtttt                                                                                                                                                >  3:648189/1‑72 (MQ=38)
                                                                                aGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGATA‑CGGCGGGTTTTTACTTTTTGAGTGGCAGAAAGATGAAAGCCTGTCCGCGagaagtgacgaaaa                                                               >  8:2153601/1‑137 (MQ=11)
                                                                                  aaaGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGGTT‑CGGCGGGTTTGTGATTTTGGAGTGGCAGATAGATGAGAGCCTGTCGCCGACACGATActctaa                                                               >  3:1635156/1‑143 (MQ=11)
                                                                                  aaaGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGACACTATACCCaaa                                                               >  7:2067225/1‑148 (MQ=25)
                                                                                     tatcaatgaCGGCCCAATACACTATACCAAAACGAAAGGGGCTTACCGCCGTGTTCCCCCCGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAaaagaaa                                                          <  3:2153713/146‑1 (MQ=11)
                                                                                      atgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGGTT‑CGGCGGGTTTTTACTTTTGGAGTGCGAGATATATTAAAGACTGTCCACGACACTATACAAAAAAGAAAGc                                                        >  1:396935/1‑151 (MQ=11)
                                                                                            gaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGCTG‑CGGCGGGTTTTTACTTTTTGAGTGGCAGATATATAAATCACTTTCGACGACAATATAAACAAAAGAAAGCGTCtt                                                   >  4:1773214/1‑150 (MQ=11)
                                                                                                  ccACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGCTG‑CGGCGGGTTTTTCCTTTTGGAGTGGGAGATAGATGACTCACTGTCGACGACACTAGCCCAAAATGAAAGCCCCTGATCggac                                            >  5:1362615/1‑149 (MQ=11)
                                                                                                   cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAAAC‑CGATT‑CGGCGGGTTTATACTATTAGAAGCGTAGAAAGATGAATGACTGTCAACGACACTATACCAAAAAGAAGTCGGCTTATcatta                                            >  8:1273604/1‑146 (MQ=11)
                                                                                                    aCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGGTT‑CGGCGGGTTTTTAATTTTGGAGTGGCAGAAAGATCAAACACTGTCCACGTCACAATACAATAAAGAAAGCGGCTTATCGCTCat                                          >  8:916809/1‑150 (MQ=11)
                                                                                                       acacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑CGATA‑CGGCGGGTTTAAAATTTTTGAGTGCCATAAATAAGAAAGAAAGACCAACAAAATATAAAAAAAAGAAATCGGCTTAtatgaaatatt                                       >  2:1073037/1‑141 (MQ=2)
                                                                                                          cTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAGACAAAAGAAAGCGGCTTATCGCTCAGTTTTa                                     >  6:1309998/1‑150 (MQ=255)
                                                                                                          cTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGATT‑CGGCGGGTTTTTGCTTTTGGAGTGGCAGAAAGATGAGTGACTGTCGACGAGACTATACCCAAAAGAAAGCCGCTGATCGCTCAGttttt                                     >  5:299011/1‑149 (MQ=11)
                                                                                                           tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGATGAATGACTGTCCACGAGACGAGAGGCAAATGACAGCGGCTTATCGCTCAGTTTTAct                                   >  3:678602/1‑150 (MQ=11)
                                                                                                           tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTGTACTTTTGGAGGGGCAGAAAGAGGAATGACTGTCGACGACACTATACGCAACAGAAAGCGGCTTATCGGTCAGTTTTAcc                                   >  3:409817/1‑151 (MQ=255)
                                                                                                            ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAAAC‑CGATG‑CGGCGGGTTTAAACTTTTTGAGTGGCATAAAGATAAAACACATTCGACGACACTAAAAACAAATGAAAGCAGAATATCGGAAAGTATTaaa                                   >  6:68311/1‑148 (MQ=2)
                                                                                                                ccAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGGAGAAAGATGATTGACTGTCCACGACACTATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATg                              >  5:1828733/1‑151 (MQ=255)
                                                                                                                 cAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTGTACTTGTGGAGGGGCAGATAGATGAAAGCGTGTCCACGACACTATACCCAAAAGAAAGCGCCTTATCGGTCAGTTATACCTGAtct                             >  5:1097559/1‑149 (MQ=11)
                                                                                                                 cAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAAAGACTGTCCACGACACGATACACAAAAGAAAGCGGCTTATCGGTAAGTGTTACGTGATGt                             >  2:2123935/1‑151 (MQ=14)
                                                                                                                  aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGt                             >  8:589634/1‑150 (MQ=39)
                                                                                                                  aaaaGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATCACTGTCCACGACACTATACCCAAAAGAAAGCGGATTATCGCTCAGTGTTAACTGATGt                             >  3:2249364/1‑150 (MQ=14)
                                                                                                                   aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAACC‑CGCTA‑CGGCGGGTTTTTAATTTTGGAGTGGCAGAAATAAGAAAGACTGTCGACGACACTATAAACAAAAGAAAGCTGATTATCGGTAAGtaattaatgatcta                            >  4:1503015/1‑137 (MQ=11)
                                                                                                                   aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGAGGAATGCGTGTCCGCGACACTATCCCCAAAAGAAAGCTGCTTATCGGTCAGTTTTACGTGATGTAc                           >  1:951522/1‑151 (MQ=11)
                                                                                                                    aagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTAACTTTTGGAGGGGCAGAAAGATGAAAGACTGTCCACGACACTATAAACAAAAGAAAGCGACTTATCGCTCAGTATTaaattatctacg                          >  6:110771/1‑140 (MQ=11)
                                                                                                                       aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTAATTTTTGAGGGGCAGATAGATGAATCGCTGTCGGAGACGCTAGAGGCAAAAGAAAGCTGGGTTTCGGTCAGTTTTACGTGATGTACgga                        >  8:1631897/1‑148 (MQ=14)
                                                                                                                        aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTAATTTTGGAGTGGCAGAAAGATGAATGACTCTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGTTCAGTTTTACCTGATGTACGtaat                      >  4:446082/1‑151 (MQ=255)
                                                                                                                         agCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                      >  7:307492/1‑150 (MQ=255)
                                                                                                                          gCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACAGTCCACGACACTATACCAAAAAGAAAGCGGCTTATCGGAAAGTTTTACCTGATGTAAGtaata                     >  5:203379/1‑150 (MQ=255)
                                                                                                                            ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATaaa                   >  7:1737683/1‑150 (MQ=255)
                                                                                                                            ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  >  7:1223406/1‑151 (MQ=255)
                                                                                                                            ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  >  4:1915199/1‑151 (MQ=255)
                                                                                                                             gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                 >  5:1642920/1‑151 (MQ=255)
                                                                                                                             gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                 >  5:749351/1‑151 (MQ=255)
                                                                                                                              cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGATGAATGACTGTCCACGACACTATAGAAAAAAGAAAGCGGCTTATCGGTAAGTTTTACCTGATGTAAGTAATAAAc                  >  4:309890/1‑149 (MQ=14)
                                                                                                                              cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  5:821816/1‑151 (MQ=255)
                                                                                                                              cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  5:120365/1‑151 (MQ=255)
                                                                                                                               ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  1:1116997/1‑151 (MQ=255)
                                                                                                                               ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  5:593093/1‑151 (MQ=255)
                                                                                                                                  tCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc            >  7:2211932/1‑151 (MQ=255)
                                                                                                                                   cGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           >  2:823484/1‑151 (MQ=255)
                                                                                                                                   cGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           >  1:1014737/1‑151 (MQ=255)
                                                                                                                                    ggTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg          >  4:791527/1‑151 (MQ=255)
                                                                                                                                     gTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc         >  7:456052/1‑151 (MQ=255)
                                                                                                                                     gTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc         >  6:587968/1‑151 (MQ=255)
                                                                                                                                      tCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg        >  4:2193249/1‑151 (MQ=255)
                                                                                                                                      tCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg        >  7:562490/1‑151 (MQ=255)
                                                                                                                                      tCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGATCcggcg        >  6:1333690/1‑151 (MQ=255)
                                                                                                                                          tttCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  8:1722525/1‑151 (MQ=255)
                                                                                                                                          tttCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  7:653728/1‑151 (MQ=255)
                                                                                                                                           ttCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt   >  7:1042928/1‑151 (MQ=255)
                                                                                                                                             cACCTGTTTT‑CGTTAAACCCGGGCTG‑CGGGGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  <  7:1509271/150‑1 (MQ=17)
                                                                                                                                             |                                                                                                                                                       
CAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑CGCTT‑CGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613629‑613919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑