BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	1,065,605	0	A	T	28.6%	451.4 / 174.9	276	intergenic (+40/‑23)	ECDH1_RS05245/ECDH1_RS05250	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base A (122/73); new base T (54/24); total (178/98)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

CAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065457‑1065754

  aaaacTCTCCAGCAAAGTAAGTACCCCGTTCAATACGTAGCTCAGCTGTACAGAATAATCGGCTACTA‑ACCCTTGGGTCTTAGGTAGGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATcagcag                                                                                                                                                    <  5:668703/151‑1 (MQ=12)

     aaTCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTat                                                                                                                                                 >  7:1527287/1‑150 (MQ=255)

            agtaaagtaaGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAATACTATATATTGAGCAAGGAG‑GGTATCC‑‑AATATCAACAGTAaaatac‑at                                                                                                                                         >  7:145976/1‑151 (MQ=11)

               aaagtaaGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑Attt                                                                                                                                       <  6:209282/150‑1 (MQ=255)

                   taagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTGGTACTAGATATGGAGTTAGTAGCGGTTG‑C‑‑AATATCAGGAGTAAATTAA‑ATTTcgcg                                                                                                                                   >  8:57150/1‑150 (MQ=17)

                            cccgcccgccGGAGCTCAGCGGGCCAGACTACGCGGCGACGA‑CCCGACCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGt                                                                                                                         <  2:1471751/143‑1 (MQ=18)

                               tGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTTGTACGTGATATGGCTTTATGAGCGGTAT‑G‑‑AATATCTGCAGTAAATTAA‑ATATCCCGCTgggtcggatgc                                                                                                                      >  7:490298/1‑141 (MQ=17)

                               tGCAGCGGTAGGTCAGCTGGCTTGACTAATCGGCTAGGATGGCGAG‑GCTCGGCGCTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAg                                                                                                                       <  7:187822/150‑1 (MQ=14)

                                gccgccGGAGCTCAGCTGGAGAGTGTACTCGGCTCCGA‑TCCGGTGGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCt                                                                                                                     <  3:1884035/147‑1 (MQ=18)

                                     cGTAGCTCAGCTGGATTGAGTACTCGGCTACGA‑ACGGCGCGGTCGGAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCt                                                                                                                <  7:1290866/151‑1 (MQ=255)

                                                 ggATAGAGTACTCGGCTACGA‑ACCGAGCGGTCGGAGTTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGTTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATagag                                                                                                        <  7:2053295/147‑1 (MQ=255)

                                                 ggATAGAGTACTCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCACCAGTAGAATAA‑ATGACCACATCCATCCGTTCACCATCTGGATTGAGTTCt                                                                                                    >  1:778206/1‑151 (MQ=18)

                                                  gttcctatcatCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACt                                                                                                    <  4:872883/140‑1 (MQ=255)

                                                            tCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAc                                                                                         >  7:1675720/1‑151 (MQ=255)

                                                                cTACGA‑ACCGTGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAgaataa                                                                                                                                            <  2:2290463/97‑1 (MQ=255)

                                                                   cGA‑ACGCAGCGCTCGGAGGTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCgg                                                                                  <  3:1140360/151‑1 (MQ=255)

                                                                   cGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTGGTACTTGATATGCATGTAGGAGGGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCCTCCGAAGATCAGCTGTTATCAGTACTCGGCTACGAACCCAGccg                                                                                  >  1:592509/1‑149 (MQ=18)

                                                                      atcgcgccgctCGGAGAGTCTAATCCTCCCTTATTCACCATCTCTTACTTTATATGTCTTTACTAGCGCTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTc                                                                                <  3:1192647/141‑1 (MQ=14)

                                                                        ccGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGAGGTAA‑C‑‑AATATCAGAAGTAAAAAAA‑AGATGCCGATGCATACGTAGCTCAGCTGTAAAGAGTAATCGGATACGAAACGAgcgggcgg                                                                              >  2:383516/1‑151 (MQ=17)

                                                                         cgtgCGGTCGGAGTGTCGAATCCTCCCTGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGa                                                                             <  8:123971/148‑1 (MQ=255)

                                                                          gtccGGTCGGAGGGTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAg                                                                            <  7:1275462/148‑1 (MQ=255)

                                                                            gCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTACTTGATATGGATGTAGTAGGGGTTG‑C‑‑AATATCAGCAGTAAAATAA‑ATATCCCGCTGCGTAAGTAGAACAGCAGGTTAGAGAACTCGGCTACGAGCAGAGCGTTCGGAGGt                                                                          >  2:1956139/1‑151 (MQ=19)

                                                                              ggTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATcagc                                                                                                                                                      >  6:1784092/1‑74 (MQ=255)

                                                                              gctcggcgcgtctaaTCCTCGCGGATGCACGATCTCTTACTTTATATGTCTTTAGTAGCTGTAT‑A‑‑AATATCAGCAGTAAATTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTc                                                                        <  1:267756/138‑1 (MQ=11)

                                                                                tCGGAGGTTCGAATCCTCCCGGATGCACCATCTGGTACTTGATATTGCTGTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCTTAGGTAGCTCTTCTGGATAGAGTGCTCGGCTACGATACGCGCGGTCGGAGGTTCg                                                                       >  5:1997056/1‑150 (MQ=17)

                                                                                tCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAGAATAAGTTTTCCCGATGCAt                                                                                                                             >  8:1389799/1‑97 (MQ=11)

                                                                                 atgagagtttaaTCCTCGCGGATTCAATATCTCTTACTTGATATGTCTTTATTAGCTTTAT‑A‑‑AATATCAGCAGTAAAATAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGaa                                                                     <  4:985344/141‑1 (MQ=11)

                                                                                  ggAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTaga                                                                                                                                                >  3:1792676/1‑74 (MQ=255)

                                                                                  ggAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATcagca                                                                                                                                                     <  6:1599611/71‑1 (MQ=255)

                                                                                      gTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGt                                                                                                                                                   >  6:2134450/1‑69 (MQ=255)

                                                                                      gTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGt                                                                                                                                                   <  5:2135375/69‑1 (MQ=17)

                                                                                           tagcgacccgcgtGCACCAGCTCTGTCTTGAGATCTCTTTACGAGCGCTAT‑A‑‑AAAATCAGCAGTAAACTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                            <  7:410006/138‑1 (MQ=11)

                                                                                              cctccCGGCTTCACCATCTCTTAGTTGATAGGGGTTTTCTCGCGTTAT‑C‑‑AATATCAGCAGTAAACTAA‑ATTTCCCTATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGc                                                        <  7:342747/151‑1 (MQ=11)

                                                                                                  ccgcaggCACCCGCTCTTACTTGATATGTCTTTAGTAGCCGGAT‑C‑‑AGTCTCACCCCTAAACTAA‑TTTTGCAGTTGCTTGCCTAGGTCAGCTGTTGAGAGCTCTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCa                                                    <  7:1782594/145‑1 (MQ=2)

                                                                                                     gATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTAGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCatat                                                 >  8:1770686/1‑151 (MQ=255)

                                                                                                       tGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtc                                               >  5:137396/1‑151 (MQ=39)

                                                                                                        gCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTACCCAAGCATCCGTAGCTAAGCTGGATAGAGTACTAGGCAAAGAACCGAGCGGTAGGAGGTTAGAATACAACCGGATTCAACATAttat                                              >  2:1147147/1‑149 (MQ=11)

                                                                                                         cACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATtcat                                             >  1:2234502/1‑149 (MQ=39)

                                                                                                             aTCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGa                                          >  1:440431/1‑150 (MQ=40)

                                                                                                              tctcTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGt                                                                                                                                                   >  4:594064/1‑45 (MQ=35)

                                                                                                               ctctTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAt                                       >  1:1412430/1‑151 (MQ=255)

                                                                                                                tctTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATc                                      >  6:991907/1‑151 (MQ=255)

                                                                                                                    aCTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTc                                   >  3:697947/1‑150 (MQ=255)

                                                                                                                       tGATACGGCTTTAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACTAAAtataca                                  >  1:2279163/1‑145 (MQ=14)

                                                                                                                        gATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAACAAATTTTCCGTAAAGATCAGCa                               >  4:134283/1‑150 (MQ=11)

                                                                                                                         atatGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAAt                             >  1:1792073/1‑151 (MQ=255)

                                                                                                                         atacGGCTTTAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCTATATCTTCatc                                >  1:1308472/1‑148 (MQ=14)

                                                                                                                          tacggCTTTAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAGATCTGGAGCa                               >  2:1299050/4‑150 (MQ=14)

                                                                                                                           atcggtagaccagCGATAT‑C‑‑AAAATCTGCACTAAAATAA‑TTTTCCCGATGCAGCCGTAGCTCAGCTTGATATACTAATCGGCTACCATCCCTAGGCGCCTCGCGTGGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGa                           <  8:1993872/140‑1 (MQ=2)

                                                                                                                           acggCTTTAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCTACATATTCCTCAAt                             >  1:929524/3‑151 (MQ=14)

                                                                                                                             ggCTTTAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGCTCCGAGATATTccgatatt                            >  8:1932837/1‑143 (MQ=14)

                                                                                                                               cTTTAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCTACATATTCAGATATGaac                         >  5:984236/1‑150 (MQ=14)

                                                                                                                                tttAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGTAATCTTGAGGAATGAggg                        >  3:2139369/1‑151 (MQ=11)

                                                                                                                                tttAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGACATATTCAGCTATGACgg                        >  5:1773232/1‑151 (MQ=11)

                                                                                                                                tttAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACGAAATATTCATAAATGaac                         >  6:49547/1‑149 (MQ=14)

                                                                                                                                tttAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGt                       >  8:525933/1‑150 (MQ=255)

                                                                                                                                 ttAGTAGCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATATTCCGAAATGaccct                       >  6:1289744/1‑147 (MQ=11)

                                                                                                                                 attatagggatat‑a‑‑AAAATCAGCAGTAAACTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTa                      <  7:986231/140‑1 (MQ=14)

                                                                                                                                  tagtagCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATc                    >  4:1738534/1‑151 (MQ=255)

                                                                                                                                  tagtagCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATc                    >  4:345093/1‑151 (MQ=255)

                                                                                                                                   agtagCGGTAT‑CAAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTAAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCATATATATTCATTAATGAAGGTa                      >  3:1070209/1‑150 (MQ=14)

                                                                                                                                      gagcgctcac‑‑AATATCTTCATTAAAATAA‑GTTTACCTATGCATCCTTATCTCAGCTGGATAGAGTACTCGTCTACGAACCGATTGGTATGAGGGTGGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGaa                 <  8:2202068/142‑1 (MQ=11)

                                                                                                                                      agctttat‑a‑‑AATATCAGCAGTAAATTAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                <  4:101279/146‑1 (MQ=255)

                                                                                                                                      agCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGa                  >  3:253324/1‑149 (MQ=255)

                                                                                                                                      agCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAAACTTCAGCAATGAAGGTATCGa                  >  7:1327746/1‑149 (MQ=255)

                                                                                                                                       gtcgggctctaaaTATCAGCAGTAAAGTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCg                   <  1:2077504/139‑1 (MQ=14)

                                                                                                                                        cGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                >  1:1647774/1‑149 (MQ=255)

                                                                                                                                        cGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAACCTTCAGCAATGAAGGTATCGAag                >  2:839542/1‑149 (MQ=38)

                                                                                                                                         ggaaT‑C‑‑AAAATCCGCCGTAAAATAC‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcctc                                                                <  1:346987/97‑1 (MQ=11)

                                                                                                                                         cggtatcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGAAATCTTCAGCAAtta                           >  1:187735/13‑138 (MQ=11)

                                                                                                                                         cggtatcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATCCGAAATCTTCAGCAATGAACGTATAGa                  >  4:2163044/13‑149 (MQ=1)

                                                                                                                                         aggtttaaaaaaatCTGCAGTAAATTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                <  2:628434/139‑1 (MQ=14)

                                                                                                                                          tctttataattatCTGCAGTAAAGTAA‑CTTTCCCGATGGATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAag                <  4:1903647/140‑1 (MQ=14)

                                                                                                                                          gTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt            >  8:1509610/1‑151 (MQ=255)

                                                                                                                                          gTAG‑C‑‑AATATAAGCATAAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGTAACCTTCAGCAATGAAGGTATCGAagagat            <  3:1619115/151‑3 (MQ=11)

                                                                                                                                           tAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt            >  8:432595/1‑150 (MQ=255)

                                                                                                                                           tAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGt            >  4:1593150/1‑150 (MQ=255)

                                                                                                                                            tatcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAACGTATCGAAGAgg             >  2:2227441/10‑151 (MQ=14)

                                                                                                                                            tatcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTACGAAATATTCAGCTATGACGGTaaaga                  >  5:1483409/10‑142 (MQ=11)

                                                                                                                                             t‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGc         >  5:55375/1‑151 (MQ=255)

                                                                                                                                              tcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTAGTACGAGATCTTCAGGAAGGAACGGATGCAAGAGGt            >  6:1084627/8‑150 (MQ=14)

                                                                                                                                              tcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTGCCGCAATGAAGGTATCGAatagct            >  1:1973306/8‑148 (MQ=11)

                                                                                                                                               cccaaacgcagcagTAAATTTA‑TTTTCCCGATGCATCCGTAGCTCAGCTGCATACTTCCCTAGTGTACTTAAGTAGTGGGCTGATGCTATAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAg          <  1:1511112/143‑1 (MQ=9)

                                                                                                                                               caaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTCCGACATCTTGAGGTAGTATGGGTTCGAAGAGGt            >  3:1384279/7‑149 (MQ=11)

                                                                                                                                               caaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACGTTATCTTGAGCAATGAGGGGTTCGAatagct            >  5:34459/7‑147 (MQ=11)

                                                                                                                                               c‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg        >  5:307961/1‑151 (MQ=255)

                                                                                                                                               c‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCg        >  4:1171293/1‑151 (MQ=255)

                                                                                                                                               ataaaaatCTGCAGTAAAATAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATACTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTa           <  3:846828/144‑1 (MQ=255)

                                                                                                                                                  aaTATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg       >  8:1642317/1‑151 (MQ=255)

                                                                                                                                                  aaTATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg       >  5:1143224/1‑151 (MQ=255)

                                                                                                                                                  aaTATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg       >  3:414424/1‑151 (MQ=255)

                                                                                                                                                   atatCAGCAGTAAAATAC‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGa                                                           <  3:475122/98‑1 (MQ=255)

                                                                                                                                                   atatCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGa                                                           >  4:475186/1‑98 (MQ=255)

                                                                                                                                                   atatCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg       >  7:2136215/1‑150 (MQ=255)

                                                                                                                                                    tatCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGa      >  2:1314831/1‑150 (MQ=255)

                                                                                                                                                    tatCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGAAATCTTCAGCAATGAAGGTATCGAAGAggaacagga      >  6:687963/1‑143 (MQ=255)

                                                                                                                                                    aatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGGATGGAAGAGGTacagga      >  1:1098478/2‑145 (MQ=14)

                                                                                                                                                     atCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATATTCAGAAATGAACGGTTAGAAGATGTACAGGaa     >  6:832646/1‑150 (MQ=11)

                                                                                                                                                       cagcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAAcc                                                                                        >  8:2128376/1‑65 (MQ=255)

                                                                                                                                                       cagcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt   >  5:1598583/1‑150 (MQ=255)

CAAAAAATCTGCAGTAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGA‑ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTAT‑C‑‑AATATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGAAATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTA  >  DH1/1065457‑1065754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`